A green, simple and low-cost approach was present to obtain N-doped C-dots using vegetables as the sole carbon source through facile one-pot hydrothermal treatment. The C-dots can serve as a suitable sensing platform for detection of Cu2+.
Background: Benign prostatic hyperplasia (BPH) is a common disease in elderly males, but its pathogenesis remains unclear. The aim of this study was to profile the proteome of the BPH rat prostates, human prostate tissues, and human cell lines for signaling changes and biomarker signatures.Methods: We used data independent acquisition mass spectrometry to perform a quantitative proteomics analysis in 7 Rats samples, 4 with normal prostate and 3 with BPH. The proteins including Glutaminyl-peptide cyclotransferase (QPCT), Rho guanine nucleotide exchange factor 37 (ARHGEF37), Filamin-C (FLNC), Galectin-7 (LGALS7) are significantly upregulated and top 10 upregulated proteins. The differentially expressed proteins (DEPs) and protein-coding genes which could be the potential therapeutic target were investigated. qPCR was used to validate the expression of DEPs in rat prostate tissues and human cell lines including BPH-1 and WPMY-1. Additionally, the gene expression profiling datasets (GSE119195) of human tissues were obtained from Gene Expression Omnibus (GEO) (https://www.ncbi.nlm.nih.gov/geo/) of the National Center for Biotechnology Information and the significant signaling pathway were found.Findings: We identified 196 DEPs in BPH and sham rats and revealed DEGs found in human tissues were mainly clustered in MAPK signaling pathways. In addition, QPCT, ARHGEF37, FLNC, LGALS7 were up-regulated in BPH samples in vivo and in vitro.Interpretation: This work finds that MAPK signaling pathways plays an important role in the development of BPH. QPCT, ARHGEF37, FLNC, LGALS7 could be significant biomarkers in BPH.Funding Information: The study was supported by the Fundamental Research Funds for the Central Universities of Wuhan University (2042021kf1041). The funders did not act as a role in manuscript design, data collection, data analysis, interpretation nor writing of the manuscript.Declaration of Interests: The authors have declared that no conflict of interest exists.Ethics Approval Statement: All animal protocols were approved by the Institutional Animal Care and Use Committee (IACUC) of Wuhan University (IACUC animal approval protocol #2018119).
The effects of ionic liquid antioxidant [UIM0Y2][C6H2(OH)3COO] on the oxidation resistance, corrosion inhibition, and lubrication of biodiesel were studied by using Rancimat method, static weight loss method, ion chromatography method, and high-frequency reciprocating friction and wear testing. The inhibition mechanism of [UIM0Y2][C6H2(OH)3COO] was explored by molecular dynamics .The results show that when the addition amount of [UIM0Y2][C6H2(OH)3COO] is 0.1%, the induction period of biodiesel is 8.39 h, which exceeded the EU standard (6 h). When the addition amount of [UIM0Y2][C6H2(OH)3COO] is 0.5%, the copper strip corrosion grade of biodiesel decreases from 3a to 1a, and the corrosion inhibition efficiency increases to 93.3%. In the lubrication performance test, the wear scar diameter of biodiesel with [UIM0Y2][C6H2(OH)3COO] addition is found to be 93.47 μm, which is only 43.5% of the wear spot diameter of the blank group, and the oil film thickness remains stable at 94.47%. After molecular dynamics calculation, [UIM0Y2][C6H2(OH)3COO] has the largest average adsorption energy for Cu and Fe.
CdSe-reduced graphene oxide (CdSe/RGO) composites were synthesized by a hydrothermal method. CdSe/RGO composites with different mass ratios were prepared. The structure and morphology of CdSe/RGO composites were analyzed by X-ray diffraction (XRD), scanning electron microscopy (SEM) and transmission electron microscopy (TEM). The synthesis of CdSe/RGO complexes was successfully demonstrated by Fourier infrared (FT-IR) and Raman spectra. CdSe nanoparticles in the CdSe/RGO composite were uniformly dispersed on the graphene surface. The study found that oxygen-containing functional groups such as hydroxyl (-OH) and carboxyl (-COOH) groups in graphene played a decisive role in the dispersion of CdSe. The third-order nonlinear optical properties of CdSe/RGO composites were measured by a single beam Z-scan technique. The experimental results showed that composites exhibited two-photon absorption and self-focusing nonlinear refraction properties. Additionally, the third-order nonlinear susceptibility of the composite material was obviously enhanced, which was mainly due to the good dispersion of CdSe nanoparticles on graphene.
The burden of kidney, bladder, and prostate cancers has changed in recent decades. This study aims to investigate the global and regional burden of, and attributable risk factors for genitourinary cancers during the past 30 years.
In this study, a Mg-Gd-Y-Zn-Zr alloy was processed by forward extrusion combined with dual-directional angular extrusion (FE-DDAE), and the corresponding microstructure evolution, grain refinement mechanism, and texture were investigated systematically. The deformed materials were subjected to a combination of three-way compressive stress and shear stress, and five different areas were selected to study the typical deformation behaviors. The results show that microstructures are heterogeneous in different areas, and the grain size is refined from 138.70 μm to 37.18 μm through continuous dynamic recrystallization (CDRX) and discontinuous dynamic recrystallization (DDRX). The block-shaped and lamellar long period stacking ordered (LPSO) phases can coordinate severe deformation through dissolution, tearing, breaking for the former, and bending, severe kinking, refining into small pieces for the latter. Moreover, the broken block-shaped LPSO can promote DRX process through particle-stimulated nucleation (PSN) mechanism, and the lamellar LPSO phases can hinder the dislocation motion to restrict DRX. A strong basal texture is observed during the initial stage of FE-DDAE process, and the basal texture intensity gradually weakens to 4.9 MRD due to the random orientations of high volume fraction of DRX grains. A mixed texture component is formed with basal planes inclined 50° from ED to TD, showing a trend of spreading.
Aberrant autophagy could promote cancer cells to survive and proliferate in prostate cancer (PCa).LncRNAs play key roles in autophagy regulatory network.We established a prognostic model, which autophagyrelated lncRNAs (au-lncRNAs) were used as biomarkers to predict prognosis of individuals with PCa.Depending on au-lncRNAs from the Cancer Genome Atlas and the Human Autophagy Database, a risk score model was created.To evaluate the prediction accuracy, the calibration, Kaplan-Meier, and receiver operating characteristic curves were used.To clarify the biological function, gene set enrichment analyses (GSEA) were performed.Quantitative real-time PCR (qRT-PCR) was employed to determine the au-lncRNAs expression in PCa cell lines and healthy prostate cells for further confirmation.We identified five au-lncRNAs with prognostic significance (AC068580.6,AF131215.2,LINC00996, LINC01125 and LINC01547).The development of a risk scoring model required the utilization of multivariate Cox analysis.According to the model, we categorized PCa individuals into low-and high-risk cohorts.PCa subjects in the high-risk group had a worse disease-free survival rate than those in the low-risk group.The 1-, 3-, and 5-year periods had corresponding areas under curves (AUC) of 0.788, 0.794, and 0.818.The prognosis of individuals with PCa could be predicted by the model with accuracy.Further analysis with GSEA showed that the prognostic model was associated with the tumor microenvironment, including immunotherapy, cancer-related inflammation, and metabolic reprogramming.Four lncRNAs expression in PCa cell lines was greater than that in healthy prostate cells.The au-lncRNA prognostic model has significant clinical implications in prognosis of PCa patient.
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