Qingyu Chen

The Wallace H. Coulter Department of Biomedical Engineering

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Shu Jia

The Wallace H. Coulter Department of Biomedical Engineering

Jeonghwan Son

Georgia Institute of Technology

Wenhao Liu

Chinese Academy of Medical Sciences & Peking Union Medical College

Parvin Forghani

Children's Healthcare of Atlanta

Lawrence C. Armand

Children's Healthcare of Atlanta

Jia Shu

Affiliated Hospital of Jining Medical University

Chunhui Xu

The Wallace H. Coulter Department of Biomedical Engineering

Cooperative Institutions

Georgia Institute of Technology

Emory University

The Wallace H. Coulter Department of Biomedical Engineering

Children's Healthcare of Atlanta

Cancer Institute (WIA)

AID Atlanta

Chinese Academy of Medical Sciences & Peking Union Medical College

Sun Yat-sen University

Central Hospital of Zibo

Binzhou Medical University

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Implementation of miniaturized modular-array fluorescence microscopy for long-term live-cell imaging

Jeonghwan Son Qingyu Chen Jia Shu

Fluorescence microscopy imaging of live cells has provided consistent monitoring of dynamic cellular activities and interactions. However, due to the limited adaptability of current live-cell imaging systems, portable cell imaging system has been adapted by broad strategies, including miniaturized fluorescence microscopy. Here, we provide a protocol for the construction and operational process of miniaturized modular-array fluorescence microscopy (MAM). The MAM system is built in a portable size (15 cm × 15 cm × 3 cm) and provides in situ cell imaging inside an incubator with a subcellular lateral resolution (~3 μm). We demonstrated the improved stability of the MAM system with fluorescent targets and live HeLa cells, enabling long-term imaging for 12 hours without the need for external support or post-processing. We believe the protocol could guide scientists to construct a compact portable fluorescence imaging system and perform time-lapse in situ single-cell imaging and analysis.

Fluorescence-lifetime imaging microscopy

Live cell imaging

Single-Cell Analysis

10.1364/opticaopen.22080692.v1

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3D imaging of hiPSC-derived cardiomyocytes with light-field microscopy

Wenhao Liu Parvin Forghani Qingyu Chen Lawrence C. Armand Chunhui Xu

We introduce light-field microscopy for volumetric imaging of 3D Human induced pluripotent stem cell-derived cardiomyocytes with high spatiotemporal resolution, realizing simultaneous recording of calcium oscillations and cellular movements in cardiac spheroids and demonstrating their synchronization.

Calcium imaging

10.1364/3d.2023.dtu5a.4

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3D imaging of hiPSC-derived cardiomyocytes with light-field microscopy

Frontiers in Optics + Laser Science 2022 (FIO, LS) (2023)

Wenhao Liu Parvin Forghani Qingyu Chen Lawrence C. Armand Chunhui Xu

We introduce light-field microscopy for volumetric imaging of 3D Human induced pluripotent stem cell-derived cardiomyocytes with high spatiotemporal resolution, realizing simultaneous calcium tracing and cell tracking of cardiomyocytes in 3D space and demonstrating their synchronization.

Tracing

Calcium imaging

10.1364/fio.2023.fm1e.3

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Implementation of miniaturized modular-array fluorescence microscopy for long-term live-cell imaging

Applied Optics (2023)

Qingyu Chen Jeonghwan Son Shu Jia

Fluorescence microscopy imaging of live cells has provided consistent monitoring of dynamic cellular activities and interactions. However, because current live-cell imaging systems are limited in their adaptability, portable cell imaging systems have been adapted by a variety of strategies, including miniaturized fluorescence microscopy. Here, we provide a protocol for the construction and operational process of miniaturized modular-array fluorescence microscopy (MAM). The MAM system is built in a portable size (15

Live cell imaging

Fluorescence-lifetime imaging microscopy

Photoactivated localization microscopy

10.1364/ao.483279

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Implementation of miniaturized modular-array fluorescence microscopy for long-term live-cell imaging

Jeonghwan Son Qingyu Chen Jia Shu

Fluorescence-lifetime imaging microscopy

Live cell imaging

Single-Cell Analysis

10.1364/opticaopen.22080692

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