Chunyan Chen

Qilu Hospital of Shandong University

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Minran Zhou

Qilu Hospital of Shandong University

Zelong Cui

Qilu Hospital of Shandong University

Jihui Jia

Shandong University

Xiaolin Yin

Shandong University

Xinwen Jiang

Qilu Hospital of Shandong University

Qingli Ji

Qilu Hospital of Shandong University

Sai Ma

Qilu Hospital of Shandong University

Lu Zhang

Qilu Hospital of Shandong University

Jiping Zeng

Shandong University

Zhenfang Liu

First Affiliated Hospital of GuangXi Medical University

Cooperative Institutions

Shandong University

Qilu Hospital of Shandong University

Sun Yat-sen University

Huazhong University of Science and Technology

Peking University

Chinese Academy of Medical Sciences & Peking Union Medical College

Shanghai Jiao Tong University

Central South University

Chinese Academy of Sciences

Southern Medical University

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Validation and Use of Predictive Scoring Systems for Molecular Responses in 5,203 Persons with Chronic Myeloid Leukemia

Blood (2022)

Xiaoshuai Zhang Bingcheng Liu Jian Huang Zhang Gong-li Xiaoli Liu

Imatinib Mesylate

10.1182/blood-2022-166431

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Changes of proteome components of Helicobacter pylori biofilms induced by serum starvation

Molecular Medicine Reports (2013)

Chunhong Shao Yundong Sun Na Wang Han Yu Yabin Zhou

Biofilm is the adaptive living mechanism of Helicobacter pylori (H. pylori) during survival and propagation. Nutrient starvation is an environmental pressure for H. pylori in vivo and in vitro. Serum starvation effectively mimics the microenvironment in which H. pylori colonizes healthy humans who carry H. pylori and patients with chronic atrophic gastritis. In addition, it also mimics the in vitro environmental pressures of H. pylori. An H. pylori biofilm was successfully induced with serum starvation. To identify novel proteins associated with biofilm formation at the early stage in H. pylori, high-resolution 2-dementional gel electrophoresis was performed to obtain the proteome profiles of spiral H. pylori and early biofilm. Differential protein spots were identified using tandem matrix assisted laser desorption ionzation time of flight mass spectrometry, which revealed 35 proteins. These proteins are associated with various biological functions, including flagellar movement, bacterial virulence, signal transduction and regulation. To verify the results, the expression of cagA at the mRNA and protein levels was examined by fluorescence quantitative PCR and western blot analysis, respectively. This study indicates that H. pylori form biofilms by initiating multiple mechanisms involving a number of signaling pathways.

Proteome

CagA

10.3892/mmr.2013.1712

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Citations (26)

Comparison of the Efficacy Among Nilotinib, Dasatinib, Flumatinib and Imatinib in Newly Diagnosed Chronic-Phase Chronic Myeloid Leukemia Patients: A Real-World Multi-Center Retrospective Study

Clinical Lymphoma Myeloma & Leukemia (2024)

Xiaoshuai Zhang Na Xu Yunfan Yang Hai Lin Bingcheng Liu

10.1016/j.clml.2024.02.008

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Citations (2)

Comment on “Predicting metastasis at initial diagnosis and radiotherapy effectiveness in patients with metastatic osteosarcoma”

Journal of Cancer Research and Clinical Oncology (2023)

Guoqing Liu Shu Huang Qian Hu Chunyan Chen

Hematology

10.1007/s00432-023-05212-0

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RBP2 Promotes Adult Acute Lymphoblastic Leukemia by Upregulating BCL2

PLoS ONE (2016)

Xiaoming Wang Minran Zhou Yue Fu Ting Sun Jin Chen

Despite recent increases in the cure rate of acute lymphoblastic leukemia (ALL), adult ALL remains a high-risk disease that exhibits a high relapse rate. In this study, we found that the histone demethylase retinoblastoma binding protein-2 (RBP2) was overexpressed in both on-going and relapse cases of adult ALL, which revealed that RBP2 overexpression was not only involved in the pathogenesis of ALL but that its overexpression might also be related to relapse of the disease. RBP2 knockdown induced apoptosis and attenuated leukemic cell viability. Our results demonstrated that BCL2 is a novel target of RBP2 and supported the notion of RBP2 being a regulator of BCL2 expression via directly binding to its promoter. As the role of RBP2 in regulating apoptosis was confirmed, RBP2 overexpression and activation of BCL2 might play important roles in ALL development and progression.

Demethylase

Retinoblastoma

Pathogenesis

10.1371/journal.pone.0152142

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Citations (7)

Arsenic Primes Human Bone Marrow CD34+ Cells for Erythroid Differentiation

Bioinorganic Chemistry and Applications (2015)

Yuanyuan Zhang Shasha Wang Chunyan Chen Xiao Wu Qunye Zhang

Arsenic trioxide exhibits therapeutic effects on certain blood malignancies, at least partly by modulating cell differentiation. Previous in vitro studies in human hematopoietic progenitor cells have suggested that arsenic may inhibit erythroid differentiation. However, these effects were all observed in the presence of arsenic compounds, while the concomitant cytostatic and cytotoxic actions of arsenic might mask a prodifferentiating activity. To eliminate the potential impacts of the cytostatic and cytotoxic actions of arsenic, we adopted a novel protocol by pretreating human bone marrow CD34+ cells with a low, noncytotoxic concentration of arsenic trioxide, followed by assaying the colony forming activities in the absence of the arsenic compound. Bone marrow specimens were obtained from chronic myeloid leukemia patients who achieved complete cytogenetic remission. CD34+ cells were isolated by magnetic-activated cell sorting. We discovered that arsenic trioxide enhanced the erythroid colony forming activity, which was accompanied by a decrease in the granulomonocytic differentiation function. Moreover, in erythroleukemic K562 cells, we showed that arsenic trioxide inhibited erythrocyte maturation, suggesting that arsenic might have biphasic effects on erythropoiesis. In conclusion, our data provided the first evidence showing that arsenic trioxide could prime human hematopoietic progenitor cells for enhanced erythroid differentiation.

Arsenic Trioxide

10.1155/2015/751013

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In-suit UIV/Vis Spectrum of Fe~(3+)-Doped Polymerized Anilin

Yunnan Chemical Technology (2006)

Chunyan Chen

In-suit UIV/Vis spectrum of Fe~3+ doped polymerized aniline in 1-methyl-2-pyrrolidone during 120h was measured.Result showed that the absorption blue-shifted obviously and Fe~3+ had remarkable doped effect on polymerized aniline.

Broad spectrum

Source

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Acupuncture combined with Chaihu Jia Longgu Muli Decoction in the treatment of post-stroke anxiety

Chunyan Chen

Decoction

Stroke

10.3877/cma.j.issn.2095-3240.2019.03.010

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The moral appeal of environmental problems in rural areas

WIT transactions on engineering sciences (2014)

Chunyan Chen Shuang Guo

10.2495/feem20130761

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[Regulatory Effect of Vitamin D on Renin Expression at Maternal-Fetal Interface].

PubMed (2022)

Chunyan Chen Zheng Libin Yanling Wang Xun Li Xueqin Zhang

To investigate the regulatory effect and mechanism of vitamin D on the local renin-angiotensin system at maternal-fetal interface in the pathological process of preeclampsia (PE).The mRNA and protein expression of renin in decidua of normal pregnancy and PE placentas was determined by RT-PCR and Western blot. Normal decidual tissues were treated with active and inactive vitamin D for 48 h in vitro and the expressions of renin and vitamin D deactivating enzyme CYP24A1 were determined by RT-PCR and Western blot. Normal decidual stromal cells and glandular epithelial cells were isolated and purified, and identified by immunocytochemical staining. RT-PCR was used to examine the mRNA of vdr, cyp27 b1, cyp24 a1, and renin in the two types of cells and in decidual tissue, and the mRNA products were subjected to gel electrophoresis. These two cell types were treated with active and inactive vitamin D in vitro and the expressions of renin and vitamin D deactivating enzyme CYP24A1 were determined by RT-PCR and Western blot. Decidual gland epithelial cells were treated with protein kinase A (PKA) activator forskolin or inhibitor H89 to explore the interaction between PKA pathway and vitamin D in the regulation of renin expression.The expression of renin in PE decidua was significantly higher than that of normal control at transcriptional and translational levels ( P<0.05). Vitamin D treatment could significantly down-regulate the expression of renin in normal decidua tissues ( P<0.05), while it significantly up-regulated CYP24A1 expression ( P<0.001). Decidual stromal cells and gland epithelial cells were successfully isolated from decidual tissue. Compared with that in decidual stromal cells, the mRNA level of vitamin D-related molecules in gland epithelial cells was more similar to that in decidual tissue. Active or inactive vitamin D treatment significantly inhibited the expression of renin in glandular epithelial cells ( P<0.05), but the expression of renin in decidual stromal cells was not affected. However, the treatment of active or inactive vitamin D in these two kinds of cells significantly increased the expression of CYP24A1 ( P<0.001). Active vitamin D could significantly inhibit the upregulation of renin by PKA agonist forskolin, and could inhibit the expression of renin through synergy with PKA inhibitor H89.The expression of renin in placental decidua is up-regulated in patients with PE, and the activation of local renin-angiotensin system at the maternal-fetal interface may be involved in the pathogenesis of PE. Vitamin D can specifically down-regulate renin expression in human decidual gland epithelial cells by competing with the PKA pathway. Vitamin D supplementation may have potential value for clinical intervention of PE.

Decidua

10.12182/20220860107

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Citations (2)