Xingwang Yang

Anhui University

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Xiao Sun

Hebei North University

Hai Jin

General Hospital of Shenyang Military Region

Yingqun Tao

General Hospital of Shenyang Military Region

Mengting Xu

Chongqing University

Shimiao Wang

General Hospital of Shenyang Military Region

Bao‐Guo Xiao

Huashan Hospital

Shun Gong

General Hospital of Shenyang Military Region

Jie‐Zhong Yu

Shanxi Datong University

Xinhong Wang

Guangzhou Medical University

Chunyun Liu

Yunnan Provincial Infectious Disease Hospital

Cooperative Institutions

Chinese Academy of Sciences

Shanxi University

General Hospital of Shenyang Military Region

State Administration of Traditional Chinese Medicine of the People's Republic of China

Chinese Academy of Medical Sciences & Peking Union Medical College

Anhui University

Huazhong University of Science and Technology

University of Chinese Academy of Sciences

Shanxi Medical University

Shanghai Jiao Tong University

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Effect of combined application of zinc, boron and molybdenum on yield and saikosaponin a, saikosaponin d contents of Bupleurum chinense

China Journal of Chinese Materia Medica (2014)

Jie Meng Xine-fu Chen Wenyu Yang Zhifei Li Yu Zhang

This research use "3414" fertilizer effect experiments to handle zinc, boron and molybdenum trace element fertilizer, determined the dry matter accumulation and content of saikosaponion a and d, to investigate the different ratio of zinc, boron and molybdenum on yield and saikosaponin a, saikosaponin d contents of Bupleurum chinense. Found The suitable ratio of zinc, boron and molybdenum play an active role on dry matter accumulation and distribution, the treatment Zn2B2Mo3 is the best one to promote the dry matter accumulation and transfer to the underground part; in a certain range, only use zinc or molybdenum can promote the yield of B. chinense, the yield of treatment Zn2B2Mo1 is the highest one. According to the results of regression analysis: in accordance with Zn 48.45 g x hm(-2), B 355.05 g x hm(-2), Mo 86.40 g x hm(-2), can obtain the yield with 3313.05 kg x hm(-2); the treatment Zn2BMo2 is most effective to promote the total saikosaponin a and d accumulated, according to the results of regression analysis: in accordance with Zn 36.15 g x hm(-2), B 343.05 g x hm(-2), Mo 106.35 g x hm(-2), the content of total saikosaponin a and d can reach 1.23%. This research first discovered the suitable ratio of zinc, boron and molybdenum can promote the yield and saikosaponin a, saikosaponin d contents on B. chinense.

BORO

Bupleurum

10.4268/cjcmm20142205

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Citations (1)

Application of modified registration for osseous fiducial marker in operation of deep brain stimulation assisted by ROSA robot

Zhonghua shenjing waike zazhi (2019)

Feng Xu Xingwang Yang Xiao Sun Xiaoqiu Li Xinhong Wang

Objective To further reduce the registration error of ROSA by the application of corrective registration for fiducial osseous marker. Methods Thirty-four patients, including 33 Parkinson’s disease patients and 1 essential tremor patient, were admitted to Department of Neurosurgery, General Hospital of Shenyang Military Area Command from November 2016 to January 2017 and underwent DBS (deep brain stimulation) operation assisted by ROSA. Each of them was registered by contact with 5-point titanium alloy markers. Each patient received 4 registration plans which were respectively named group A, group B, group C and group D. The virtual probe circles in group A were tangent to the end of the marker image on CT. The virtual probe circles in group B, group C and group D were individually moved by 0.25 mm, 0.50 mm and 0.75 mm to the marker tip along the long axis based on group A. The registration errors in each group were documented and 4 sets of error data were analyzed by variance analysis. Results The registration errors in group A ranged from 0.47 mm to 0.77mm, with an average of 0.60±0.08 mm. The registration errors in group B ranged from 0.15 mm to 0.68 mm, with an average of 0.39±0.12 mm. The registration errors in group C ranged from 0.11 mm to 0.39 mm, with an average of 0.27±0.08 mm. The registration errors in group D ranged from 0.22 mm to 0.66 mm, with an average of 0.43±0.10 mm. The population means in the 4 groups were not completely equal by variance analysis (F=68.024, P<0.001). Significant difference was identified in registration errors between any 2 groups except for that between the group B and D. The registration error in group C was the smallest and that in group A was the largest. The registration errors in group B and D were between them. Conclusions Registration error decreases when the virtual probe circle is moved by 0.50 mm to the tip of markers along the long axis of markers in the DBS operation assisted by ROSA. The modified registration plan could improve the accuracy of DBS operation. Key words: Deep brain stimulation; Robotics; Registration error

Fiducial marker

Image registration

10.3760/cma.j.issn.1001-2346.2019.01.014

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A distinct three-helix centipede toxin SSD609 inhibits Iks channels by interacting with the KCNE1 auxiliary subunit

Scientific Reports (2015)

Peibei Sun Fangming Wu Ming Wen Xingwang Yang Chenyang Wang

Abstract KCNE1 is a single-span transmembrane auxiliary protein that modulates the voltage-gated potassium channel KCNQ1. The KCNQ1/KCNE1 complex in cardiomyocytes exhibited slow activated potassium ( I ks ) currents. Recently, a novel 47-residue polypeptide toxin SSD609 was purified from Scolopendra subspinipes dehaani venom and showed I ks current inhibition. Here, chemically synthesized SSD609 was shown to exert I ks inhibition in extracted guinea pig cardiomyocytes and KCNQ1/KCNE1 current attenuation in CHO cells. The K + current attenuation of SSD609 showed decent selectivity among different auxiliary subunits. Solution nuclear magnetic resonance analysis of SSD609 revealed a distinctive three-helix conformation that was stabilized by a new disulfide bonding pattern as well as segregated surface charge distribution. Structure-activity studies demonstrated that negatively charged Glu19 in the amphipathic extracellular helix of KCNE1 was the key residue that interacted with SSD609. The distinctive three-helix centipede toxin SSD609 is known to be the first polypeptide toxin acting on channel auxiliary subunit KCNE1, which suggests a new type of pharmacological regulation for ion channels in cardiomyocytes.

Helix (gastropod)

Scorpion toxin

10.1038/srep13399

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Citations (11)

MiR-509-5p improves the proliferative and invasive abilities of papillary thyroid carcinoma cells by inhibiting SFRP1

Archives of Medical Science (2019)

Chunxiao Yang Yingluan Wang Wen‐Yi Yang Yujun Gao Bo Zhao

Our study was conducted to prove that miR-509-5p improved the proliferative and invasive abilities of papillary thyroid carcinoma (PTC) cells through inhibiting SFRP1 expression.QRT-PCR was conducted in order to detect the miR-509-5p expression levels in PTC and normal tissues. The miR-509-5p and SFRP1 mRNA expression levels in PTC cell lines K1, TPC-1, BCPAP and the human normal thyroid cell line HT-ori3 were also detected by qRT-PCR. The transfection was performed using Lipofectamine and lentiviral vectors. Pgcsil-008 was used as the SFRP1 gene vector. Western blot and dual luciferase reporter gene assay were conducted to investigate miR-509-5p's direct regulation on SFRP1. MTT, clone formation, and Transwell assays were adopted to investigate the biological behaviors of PTC cells. TCF/LEF luciferase assays were used to prove that miR-509-5p influenced the Wnt/β-catenin signaling pathway by regulating SFRP1.MiR-509-5p was overexpressed in PTC cells and tissues in which SFRP1 was down-regulated. MiR-509-5p bound to the 3'-UTR of SFRP1 and therefore partially weakened the proliferative, migrating and invasive activities of PTC cells. MiR-509-5p promoted activation of the Wnt/β-catenin signaling pathway through down-regulating SFRP1.MiR-509-5p improved the proliferative, migrating and invasive abilities of PTC cells through inhibiting SFRP1 expression.

Lipofectamine

MTT assay

10.5114/aoms.2019.85904

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Citations (4)

IL-17a accelerates inflammatory response after hepatic ischemia-reperfusion injury via mitochondrial-driven hepatocyte apoptosis

Xingwang Yang C Li J Liu H Liu YY Ma

Inflammatory response

Source

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The Therapeutic Potential of Rho Kinase Inhibitor Fasudil Derivative FaD-1 in Experimental Autoimmune Encephalomyelitis

Journal of Molecular Neuroscience (2014)

Yongfei Zhao Xiang Zhang Zhibin Ding Xingwang Yang Hui Zhang

Myelin oligodendrocyte glycoprotein

Fasudil

Encephalomyelitis

10.1007/s12031-014-0411-7

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Citations (14)

Significance of threshold change of abnormal muscle response in microvascular decompression for hemifacial spasm

Zhonghua shenjing waike zazhi (2017)

Jun Lin Feng Xu Guanqian Yuan Junhe Wang Xingwang Yang

Objective To investigate the intraoperative threshold change of abnormal muscle response (AMR) and its relationship to clinical outcome in microvascular decompression (MVD) for hemifacial spasm (HFS). Methods A retrospective analysis was conducted on 67 HFS patients who underwent AMR monitoring during MVD at Neurosurgery Department, General Hospital of Shenyang Military Command, from June 2015 to March 2016. The clinical efficacy was evaluated at two time points: immediately following operation and 1 year post surgery. Threshold change of AMR from pre-decompression to decompression was documented and its relationship to clinical outcomes was analyzed. Results The AMR threshold in 67 cases before skin incision was 0.1-19.0 mA (median: 3.6 mA). The AMR disappeared completely in 43 cases after decompression and remained in 24 cases. The threshold of remaining AMR after decompression was 2.2-84.0 mA (median: 18.7 mA). The threshold increased less than 1 time in 9 cases and at least 1 time in 15 cases. All patients were followed up for 1 year and 60 cases (89.6%) were cured and 7 cases (10.4%) were not. Out of 43 cases with disappearance of AMR, 42 (97.7%) were cured which included 40 (93.0%) with immediate remission post surgery. Among the 24 cases whose AMR did not disappear after decompression, 18 (75.0%) were cured including 10 with immediate remission and 8 with delayed effect from 10 day to 11 months (median: 2.5 months) post surgery. Immediate and 1-year postoperative clinical outcomes were significantly different between the group with AMR disappearance and that without AMR disappearance (both P<0.05). In the group with remaining AMR, the curing rate was higher in cases with threshold increase of AMR of at least 1 time compared with that in those with threshold elevation of less than 1 time, which were 14/15 and 4/9, respectively. The difference was statistically significant (P=0.015). Conclusions During MVD for HFS, the disappearance of AMR seems to be associated with better outcome and delayed remission is more likely to occur in those without AMR disappearance. The threshold increase of at least 1 time post decompression compared with preoperative (prior to skin incision) level might be related to better clinical outcome. Key words: Hemifacial spasm; Microvascular decompression; Abnormal muscle response; Threshold; Prognosis

Hemifacial spasm

Microvascular Decompression

10.3760/cma.j.issn.1001-2346.2017.09.009

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Short-term effect of deep brain stimulation on pain associated with Parkinson′s disease

Mengting Xu Yingqun Tao Hai Jin Shun Gong Xingwang Yang

Objective To investigate the short-term outcome of deep brain stimulation (DBS) for the treatment of pain associated with PD (Parkinson′s disease). Methods The clinical data of 47 cases of PD patients with pain symptoms admitted to Neurosurgery Department of Northern Theater Command General Hospital from January 2017 to June 2018 were retrospectively analyzed. A total of 35 patients underwent STN (subthalamic nucleus)-DBS and 12 patients underwent GPi (globus pallidus internus)-DBS. The stimulation contact selection and parameter adjustment were carried out at 3 weeks post operation. Visual analogue scale (VAS) was used to evaluate the degree of preoperative and postoperative pain severity. Results The VAS [median (upper and lower quartiles)] were 1.0 (0.5-1.4) and 1.0(0.7-1.6) respectively at 3 months and 6 months post operation in 47 patients, which was statistically different from that of pre-operation 4.0(4.0-8.0) (both P<0.001). At 3 months post operation, the improvement rate of 35 patients undergoing STN-DBS was (79.5±26.3)%, which was not statistically different from that of 12 patients undergoing GPi-DBS [(76.9±28.4)%] (P=0.78). The improvement rate of 35 patients receiving STN-DBS was (75.1±27.4)% 6 months after operation, which was not statistically different from that of 12 patients receiving GPi-DBS [(82.8±28.9)%] (P=0.86). Conclusion Deep brain stimulation could effectively relieve PD-related pain. There seems no significant difference between GPi-DBS and STN-DBS in the treatment outcome of PD-related pain. Key words: Parkinson disease; Pain; Deep brain stimulation; Treatment outcome

Subthalamic Nucleus

10.3760/cma.j.issn.1001-2346.2019.12.006

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An exploratory investigation of optimal cortical puncture site in ROSA-assisted operation of STN-DBS

Zhonghua shenjing waike zazhi (2018)

Xingwang Yang Yingqun Tao Hai Jin Feng Xu Xiaoqiu Li

Objective To explore the optimal cortical puncture site in deep brain stimulation of subthalamic nucleus (STN-DBS). Methods Fifty patients of Parkinson’s disease (PD) were enrolled into this study who were consecutively admitted to Department of Neurosurgery, General Hospital of Shenyang Military Command from September 2016 to April 2017 and underwent bilateral STN-DBS (100 electrodes implanted) assisted by the ROSA (robotized stereotactic assistance) system. CT angiography and MRI fused images were used to avoid the blood vessels in plan of surgical trajectory. Data of microelectrode recording (MER) in 100 trajectories were documented, out of which 80 trajectories with MER≥5 mm were selected. Cartesian coordinate system was established in the 3D image of ROSA. The distances were measured which were from the cortical entry point to the median sagittal line and from the cortical entry point to projected line of coronal suture of the skull onto the cortex. Moreover, the reference range was calculated and the optimal cortical puncture site was decided. Results There was no case of cerebral hemorrhage in this series. For those electrode implantation with MER ≥5 mm, distances from the entry point to the median sagittal line were decided to be 50.8 mm (maximal), 29.6 mm (minimal) and 37.9±5.0 mm (average). The distances between the cortical entry point to projection of coronal suture were 30.3 mm (maximal), -8.5 mm (minimal) and 9.0±6.5 mm (average). Conclusion In STN-DBS, choosing cortical puncture site within the area (32.9-42.8 mm lateral to the midline and 2.5-15.5 mm anterior to the coronal suture) might have greater chance of achieving satisfactory electrophysiological recordings, which could also be useful in operations assisted by the traditional stereotactic frame. Key words: Deep brain stimulation; Subthalamic nucleus; Robotized stereotactic assistant; Cortical puncture point; Microelectrode recording

Subthalamic Nucleus

Coronal suture

Stereotaxy

10.3760/cma.j.issn.1001-2346.2018.03.014

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Assessment of Deep Brain Stimulation Implantation Surgery: A Practical Scale

World Neurosurgery (2019)

Shun Gong Yingqun Tao Hai Jin Xiao Sun Yang Liu

Brain stimulation

10.1016/j.wneu.2019.11.117

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Citations (6)