Akhilesh Kumar Mishra

Indian Institute of Technology Madras

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Basdeo Kushwaha

National Bureau of Fish Genetic Resources

Sanjeev Kumar

National Bureau of Fish Genetic Resources

Ravindra Kumar

National Bureau of Fish Genetic Resources

Vishwamitra Singh Baisvar

National Bureau of Fish Genetic Resources

Mahender Singh

National Bureau of Fish Genetic Resources

W. S. Lakra

Central Institute of Fisheries Education

Neha Singh

Vardhman Mahavir Medical College & Safdarjung Hospital

Vindhya Mohindra

National Bureau of Fish Genetic Resources

Shreya Srivastava

National Bureau of Fish Genetic Resources

Ravindra Kumar

National Bureau of Fish Genetic Resources

Cooperative Institutions

National Bureau of Fish Genetic Resources

Indian Council of Agricultural Research

Central Marine Fisheries Research Institute

Banaras Hindu University

Czech Academy of Sciences, Biology Centre

Institute of Plant Molecular Biology

University of Lucknow

United Arab Emirates University

Czech Academy of Sciences

Institute of Engineering

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Mitochondrial DNA markers for population studies in Labeo rohita

Indian Journal of Fisheries (2002)

Vindhya Mohindra M Palanichami Akhilesh Kumar Mishra A G Ponnaiah

For the identification of population genetic markers in rohu Labeo rohita, universal mitochondrial DNA primers of ND1/16s rRNA and ND5/6 genes were tested and found to amplify particular fragments. Restriction digestion of the amplified products showed polymorphism suggesting its potential as a genetic marker for population studies.

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Phylogenetic analysis of some Puntius species based on 18S rDNA

European Journal of Biotechnology and Bioscience (2016)

Saroniya Rk Nagpure Ns Saksena Dn Basdeo Kushwaha Ravindra Kumar

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Establishment and Characterization of Eye Muscle Cell Line from Snow Trout, Schizothorax richardsonii (Gray, 1832), a Vulnerable Coldwater Fish, for In Vitro Studies

Turkish Journal of Fisheries and Aquatic Sciences (2020)

Sanjeev Kumar Pankaj Soni Neha Singh Ravindra Kumar Shreya Srivastava

Schizothorax richardsonii, commonly called snow trout is a vulnerable coldwater fish distributed in India and adjoining countries, is an important resource as food, ornamental and game fishery. A cell line, designated as SREM-1, has been established from the eye muscle of S. richardsonii using explant method and cultured in Leibovitz’s L-15 medium. The growth studies at 20-32°C temperatures and 5-20% of fetal bovine serum (FBS) concentrations revealed that SREM-1 cells exhibited optimal growth at 28°C in L-15 medium containing 20% FBS. Cell type was confirmed primarily as epithelial in nature by immuno-phenotyping assay and was sub-cultured for >55 times since its development. The origin of the cell line was confirmed by sequencing of cytochrome oxidase c subunit I and 16S rRNA genes. SREM-1 cell line is free from mycoplasma contamination and has been cryopreserved at different passage levels with revival efficiency of 70-80% after 6 months. The SREM-1 cell line was successfully transfected and also employed for evaluating in vitro cytotoxicity against a heavy metal, mercuric chloride. The newly established cell line of this vulnerable species would be useful as a model for aquatic toxicological and transfection studies.

Fetal bovine serum

10.4194/1303-2712-v21_2_05

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Mitochondrial 16S rRNA gene-based evolutionary divergence and molecular phylogeny ofBariliusspp.

Mitochondrial DNA (2013)

Ajay Singh Ravindra Kumar Mahender Singh Akhilesh Kumar Mishra U.K. Chauhan

Barilius is an important genus of fish family Cyprinidae, which comprises 22 species from Indian region. This study aimed to characterize five commonly occurring Bariline species, for example, Barilius bakeri. Barilius gatensis. Barilius vagra. Barilius bendelisis and Barilius tileo, across the country using partial mitochondrial 16 S rRNA gene, to estimate the order of inter- and intra-species divergence among these species and to establish phylogenetic and evolutionary relationships. The average inter-specific divergence was estimated as 7.10%. Intra-specific divergence of 0.20% and 0.10% was observed in B. gatensis and B. bendelisis that exhibited three and two haplotypes with 0.70 and 0.60 haplotype diversities, respectively. The NJ and MP phylogenetic trees were constructed using 16 S rRNA sequences along with sequences of the other five Indian cyprinid species retrieved from NCBI. Phylogenetic trees revealed five discrete branches each as a distinct species of the genus and exhibited identical phylogenetic relationship with other cyprinids. The study provided adequate information to distinguish the five Barilius species and indicated the suitability of 16S rRNA gene sequences in genetic divergence and phylogenetic studies.

Genetic divergence

Divergence (linguistics)

10.3109/19401736.2013.815168

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Redescription, new distribution record, DNA sequence and length-weight relationship of the Eel-loach Pangio pangia (Cypriniformes: Cobitidae) in the River Ganges Basin, India

UNED Research Journal (2013)

Uttam Kumar Sarkar Anurag Dabas Gulfishan Khan Vineet Kumar Dubey R. Kumar

The Eel-loach Pangio pangia has never been reported from East India in the River Ganges. In August, 2011, we collected a total of 39 individuals of this species during fish diversity explorations in River Ganges at Narora. This is the first record for Uttar Pradesh (North India). We present additional diagnostic features and description for this species previously known only from very old descriptions generally based on preserved specimens. Additionally, a molecular signature of the internal transcribed spacer 2 (ITS2) region between 5,8S and 28S ribosomal RNA genes was sequenced and submitted to NCBI (Accession No. JQ619873). The length-weight relationship showed allometric growth as b value of overall length-weight relation was 2,71 at p<0,00 which, ranged from 2,65 to 2,76. The substantial threats and issues regarding unawareness of this understudied fish species are highlighted to help sustaining fish biodiversity in India. KEY WORDSNew distribution, Pangio pangia, length weight relationship, River Ganges, India.

Cypriniformes

Cobitidae

Allometry

Actinopterygii

10.22458/urj.v5i1.250

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Corrigendum to “An updated comprehensive review of camel milk: Composition, therapeutic properties, and industrial applications” [Food Biosci. 62 (2024) 105531]

Food Bioscience (2024)

Ayman Khaliq Akhilesh Kumar Mishra Anuj Niroula Waqas N. Baba Muhammad Nouman Shaukat

10.1016/j.fbio.2024.105633

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An Updated Comprehensive Review of Camel Milk: Composition, Therapeutic Properties, and Industrial Applications

Food Bioscience (2024)

Ayman Khaliq Akhilesh Kumar Mishra Anuj Niroula Waqas Baba Muhammad Nouman Shaukat

10.1016/j.fbio.2024.105531

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Green Technologies for Pesticide Contaminated Soil and Water

Ahmad Rabbani Akhilesh Kumar Mishra Nishu Goyal Smriti Arora

The use of various agrochemicals or pesticides has undoubtedly increased agricultural crop productivity and protect crops from different types of pests and diseases. It also provides an economic advantage to farmers. At the same time, because of the overuse of pesticides to satisfy the demands of an ever-increasing population, environmental pollution is also increasing which poses a significant threat to environmental and human health. To tackle this problem, there are several eco-friendly, competent, efficient, and inexpensive emerging technologies for pesticide degradation and detoxification, known as bioremediation (including phytoremediation). Bioremediation is a multidisciplinary approach that uses the catabolic potential of bacteria, fungi, algae, and other organisms for biodegradation and detoxification. This chapter will focus on different aspects, limitations, and challenges associated with bioremediation techniques and, finally, assess the possible uses of these remedial methods for pesticide elimination or reduction from the soil and water ecosystem.

Agrochemical

Environmentally Friendly

Environmental Pollution

10.1002/9781119792888.ch5

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Change in the Office Layout and its Repercussions at Zoombrain Technologies

Delhi Business Review (2020)

Ashima Thakur Vikas Tyagi Akhilesh Kumar Mishra Paras Batra

10.51768/dbr.v21i1.211202018

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