Li Guo

Jilin Agricultural Science and Technology University

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Shipeng Cheng

University of Chinese Academy of Sciences

Yongjun Wen

Inner Mongolia Agricultural University

Feng-Xue Wang

Inner Mongolia Agricultural University

Shuqin Zhang

Fudan University

Jianwei Wang

Chinese Academy of Medical Sciences & Peking Union Medical College

Hongwei Zhu

Ludong University

Na Sun

Dalian Polytechnic University

Guy Vernet

Institut Pasteur de Bangui

Gláucia Paranhos‐Baccalà

bioMérieux (United States)

Yanling Yang

Washington University in St. Louis

Cooperative Institutions

Chinese Academy of Agricultural Sciences

Institute of Special Animal and Plant Sciences

Chinese Academy of Medical Sciences & Peking Union Medical College

Chinese Academy of Sciences

Sichuan University

Ministry of Agriculture and Rural Affairs

Jilin University

Huazhong University of Science and Technology

Capital Medical University

Chongqing Medical University

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Performance of adenosine deaminase in detecting paediatric pleural tuberculosis: a systematic review and meta-analysis

Annals of Medicine (2022)

Feiyang Na Yannan Wang Hui Yang Li Guo Xuan Liang

Background Paediatric pleural tuberculosis (TB) is a paucibacillary disease, which increases the difficulty of examination. We aimed to assess the performance of pleural fluid adenosine deaminase (ADA) in the detection of paediatric pleural TB.Methods PubMed, Web of Science Core Collection, Embase and Cochrane Library databases were searched up to 20 December 2021. We used the bivariate and hierarchical summary receiver operating characteristic models to compute pooled estimates for the overall diagnostic accuracy parameters of ADA for diagnosing paediatric pleural TB.Results Eight studies, including 290 pleural fluid samples, met the inclusion criteria. The pooled sensitivity of ADA was 0.85 (95% CI: 0.78–0.90, I2: 55.63% < 75%) for detecting patients with paediatric pleural TB. A total of 262 pleural fluid samples from four studies were included to differentiate patients with paediatric pleural TB from controls. At a unified cut-off value of 40 U/L, the pooled sensitivity, specificity, positive likelihood ratio, negative likelihood ratio, diagnostic odds ratio and area under the summary receiver operating characteristic curve of ADA were 0.89, 0.58, 2.09, 0.20, 10.48 and 0.89, respectively.Conclusions At a cut-off value of 40 U/L, the overall performance of ADA was good for detecting paediatric pleural TB, with relatively high sensitivity and low specificity. Key messageAccurate identification of paediatric pleural TB will help eliminate TB in children. At a cut-off value of 40 U/L, the overall performance of ADA was good for detecting paediatric pleural TB, with relatively high sensitivity and low specificity.

Diagnostic odds ratio

10.1080/07853890.2022.2140452

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Citations (7)

Genetic diversity of bovine viral diarrhea viruses in commercial bovine serum batches of Chinese origin

Infection Genetics and Evolution (2014)

Shuqin Zhang Bin Tan Li Guo Feng-Xue Wang Hongwei Zhu

10.1016/j.meegid.2014.07.021

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Citations (15)

Research advances on major pattern-recognition receptors and their innate immune responses against virus invasion in animals

Shengming kexue (2011)

Li Guo

The innate immune system is responsible for early detection of potentially pathogenic microorganisms and plays an important role in the process of host defence against virus invasion.Pattern-recognition receptors(PRRs) are important components of the innate immune system,enabling early detection of pathogenic infection.In recent years,the various members of the different PRR families are recognized and become currently a hot subject of research in veterinary medicine,which provide important information about the role of PRRs in the complex mechanism of the innate immune against viruses in animals.In the present article,the molecular traits of Toll-like receptors(TLRs) and RIG-I like receptors(RLRs) and their innate immune responses against virus infection in animals are briefly introduced.

Immune receptor

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Cloning and Expression Levels of Genes Relating to the Ovulation Rate of the Yunling Black Goat1

Biology of Reproduction (2008)

Huijie Cui Sumei Zhao M.L. Cheng Li Guo R.Q. Ye

This study investigated the nucleotide sequences and tissue expression levels of genes relating to the ovulation rate in Yunling black goats, a famous Yunnan province, China, local breed with low fecundity. Five genes, FSHB, FSHR, BMP15, BMPR1B, and ESR2, were investigated; the complete cDNA sequences of these genes were 390, 2088, 1185, 1509, and 1585 bp, respectively, and compared with Boer goats (a more fecund breed), the sequence identities were 99%, 99%, 99%, 100%, and 99%, respectively. There were two base differences in FSHB and BMP15, four in FSHR, and three in ESR2. There were fewer follicles and oocytes in Yunling black goats than in Boer goats. Expression levels of FSHB, FSHR, and BMP15 genes in Yunling black goats were lower, and expression levels of BMPR1B and ESR2 genes were higher. Serum FSH content was lower in Yunling black goats, but serum estrogen content was higher. Protein expression levels of FSHR, BMP15, BMPR1B, and ESR2 in ovary and pituitary correlated positively with gene mRNA expression levels. In Yunling black goats, the mRNA expression levels of FSHB, FSHR, and BMP15 positively correlated with litter size, but those of BMPR1B and ESR2 correlated negatively. Together, base changes and variations in mRNA and protein expression levels of genes relating to the ovulation rate result in low fecundity in the Yunling black goat. Reduced BMP15 and FSHR levels may be related to the observed fewer oocytes and, consequently, fewer follicles.

Cloning (programming)

10.1095/biolreprod.108.069021

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Gradual replacement of all previously circulating respiratory syncytial virus A strain with the novel ON1 genotype in Lanzhou from 2010 to 2017

Medicine (2019)

Xuan Liang Donghai Liu De Chen Li Guo Hui Yang

Abstract ON1 is a novel genotype of human respiratory syncytial virus (HRSV) subtype A, in children with acute respiratory tract infections (ARTIs). However, there is not much data on the prevalence and clinical and molecular characterization in China. Our study is based on the children who had respiratory infections positive for RSV-A admitted by Gansu Provincial Maternity and Child-care Hospital in Lanzhou (northwestern China) during the last 7 epidemic seasons from 2010 to 2017. In our study, different strains of the novel RSV-A genotype ON1, first identified in Canada in December 2010, were first detected in Gansu Provincial Maternity and Child-care Hospital in August 2012 and then followed by an abrupt expansion in the number of ON1 variants in the beginning of 2014 and eventually replaced all other RSV-A strains from 2015 to 2017. ON1 is characterized by a 72-nt duplication in the C-terminal region of the highly variable attachment glycoprotein (G), predicted to lengthen the polypeptide with 24 amino acids, including a 23-aa duplication, which likely changes antigenicity. New N-glycosylation sites occurred within the 23-aa duplication and 24-aa insertion of the ON1 viruses in our study. Notably, RSV infections occurred later, but peaked sooner from the 2014/2015 to 2016/2017 epidemic seasons, compared with the previous 4 seasons. Our study concluded that genotype ON1 has caused larger outbreaks and became the predominate genotype for HRSV subgroup A in Lanzhou from 2013 to 2017, and became the sole genotype of RSV-A in 2015/2016 and 2016/2017. Our data indicate that northwest of China and the world will eventually be dominated by the ON1 RSV-A genotype, including the possibility for vaccine development. Based on trends seen in RSV-B BA genotype, which predominated for decades, there is a possibility to develop a vaccine for children in the next 10 years.

10.1097/md.0000000000015542

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Calcitonin Gene-Related Peptide and its Receptor Plays Important Role in Nociceptive Regulation in the Arcuate Nucleus of Hypothalamus of Rats with Inflammatory Pain

SSRN Electronic Journal (2022)

Laixi Luo Wentao Qi Yuyan Zhang Li Guo Jinyi Wang

The present study has explored the role of calcitonin gene-related peptide (CGRP) and its receptor in inflammatory pain modulation in arcuate nucleus of hypothalamus (ARC). Our study demonstrated that intra-ARC injection of CGRP induced antinociceptive effects to naïve rats and rats with inflammatory pain, the effect could be inhibited by the selective CGRP receptor antagonist CGRP8-37. Interestingly, the CGRP-induced antinociception effect was decreased in rats with inflammatory pain compared to naïve rats. Similarly, we found that calcitonin receptor like receptor (CLR), a main component of CGRP receptor, had a low decreased expression levels in the ARC regions of rats with inflammatory pain. The CGRP-induced antinociceptive effect was significantly impaired after reducing CLR expression by intra-ARC administration of CLR targeted siRNA. These findings demonstrated that CGRP might play a crucial role in nociceptive modulation in the ARC during inflammatory pain, which was mediated by CGRP receptor in the ARC. This study shed light upon CGRP and its receptor interaction might be valuable strategies for the alleviation of inflammatory pain.

Calcitonin receptor

10.2139/ssrn.4233850

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Comment on: Rabies‐infected dogs at slaughterhouses: A potential risk of rabies transmission via dog trading and butchering activities in Vietnam

Zoonoses and Public Health (2022)

Yanlong Cong Junfeng Li Yixue Sun Fengjie Wang Lifeng Zhao

10.1111/zph.12917

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Survey of human and bovine tuberculosis infection on dairy farms in southwestern China

Research Square (Research Square) (2019)

Shu Zhang Chuang Chen Zhengyuan Rao Aiguo Yang Li Guo

Abstract Background Tuberculosis is a zoonotic chronic infectious disease that can be transmitted through respiratory secretions, causing simultaneous infection in both people and cattle. The aim of our study was to identify the co-infections of Mycobacterium tuberculosis between human and cattle in dairy farms in Sichuan Province, southwest China.Results We selected 6 large-scale dairy farms and 5 small-scale dairy farms in Sichuan Province, southwest China, as research sites. A total of 378 dairy farm workers (except one pregnant woman)were screened for tuberculosis symptoms and examined by X-ray radiographs. One worker was diagnosed with tuberculosis, but the sputum culture result was negative, and no strain was obtained. The purified protein derivative (PPD) skin tests of 10224 dairy cows showed that 99 cows were positive, with a positive rate of 9.7‰. The Oesophageal-Pharyngeal (OP) secretions of PPD-positive cows were cultured, and 21 strains were obtained. After amplifying and sequencing the 16SrDNA, hsp65 and rpoB genes and the ITS region, sequence alignment in BLAST showed that these strains were nontuberculous mycobacteria (NTM)(18 Mycobacterium nonchromogenicum , 1 Mycobacterium hiberniae , 1 Mycobacterium arupense and 1 Mycobacterium chitae ).Conclusion This study indicated that PPD-positive cows on dairy farms were infected with NTM rather than Mycobacterium bovis . Cross-infection of tuberculosis between humans and cows on dairy farms has been controlled.

Nontuberculous Mycobacteria

rpoB

Bovine tuberculosis

GeneXpert MTB/RIF

10.21203/rs.2.16233/v1

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Volatile Cmpounds and Aroma Characteristics of Jingui Green Tea and Jingui Flowers

DOAJ (DOAJ: Directory of Open Access Journals) (2022)

Li Guo Xiaojun Hu Wenjiao HU Yue Zhang Yin ZHU

10.13386/j.issn1002-0306.2021080138

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