Fang Chen

Huazhong Agricultural University

Author Statistics

Papers

Citation

H-Index

i-10 index

Research Trends

Author Order

Document Type

Co-Authors

Chang-Chun Song

Huazhong Agricultural University

Ying Xu

Hunan Agricultural University

Jin He

Huazhong Agricultural University

Zhi Luo

Qingdao National Laboratory for Marine Science and Technology

Shan‐Ho Chou

National Chung Hsing University

Shenghua Wang

XinHua Hospital

Lin Tang

Southwest University

Shu-Wei Chen

Sun Yat-sen University

Chong-Chao Zhong

Huazhong Agricultural University

Xinfeng Li

Henan University

Cooperative Institutions

Huazhong Agricultural University

Chinese Academy of Sciences

Sichuan University

University of Chinese Academy of Sciences

Chinese Academy of Agricultural Sciences

Chinese Academy of Medical Sciences & Peking Union Medical College

Peking University

Shanghai Jiao Tong University

Huazhong University of Science and Technology

Fudan University

Author Statistics

Papers

Citation

H-Index

i-10 index

Research Field

Overexpression and Characterization of the Gene Encoding 1,3-Propanediol Oxidoreductase of Citrobacter freundii

Key engineering materials (2014)

Xianghui Qi Jing Zhu Yane Luo Jing Lin Xu Wang

Glycerol can be biologically converted to 1,3-propanediol (1,3-PD), a key raw material required for the synthesis of polytrimethylene terephthalate and other polyester fibers. 1,3-propanediol oxidoreductase (PDOR) is the rate-limiting enzyme in 1,3-PD synthesis biologically pathway. In present study, the dhaT gene encoding PDOR was cloned from Citrobacter freundii and overexpressed in E. coli BL21. The recombinant enzyme was purified by nickel-chelate chromatography combined with gel filtration to study the enzymatic characterization. Specific activity of recombinant PDOR was 55.2 U/mg. K m and V max values were 8.9 mM, 40.2 U/mg respectively. Holoenzyme of PDOR maybe a decamer of which a monomer has a molecular mass of 43 kDa. This research may form a basis for the future work on biological synthesis of 1, 3-PD.

Citrobacter freundii

1,3-Propanediol

Molecular mass

Citrobacter

10.4028/www.scientific.net/kem.636.121

Cite

Citations (0)

High level expression and purification of bioactive human α-defensin 5 mature peptide in Pichia pastoris

Applied Microbiology and Biotechnology (2009)

Aiping Wang Song Wang Mingqiang Shen Fang Chen Zhongmin Zou

Pichia

10.1007/s00253-009-2020-x

Cite

Citations (42)

Impact of high-hydrostatic pressure and thermal processing on the antioxidant profiles and capacity of tomato juice during storage

Food Innovation and Advances (2023)

Xuehua Wang Li Dong Chen Ma Zhe Wang Xiao Hu

While high-hydrostatic pressure (HHP) has successfully been applied to the pasteurization of fruit and vegetable juice beverages, their quality-stable shelf life during storage has not been fully elucidated. Therefore, we investigated the effect of HHP (550 MPa/10 min) treatment on polyphenols, carotenoids, ascorbic acids, and antioxidant capacity in tomato juice and their changes during 4-week refrigerated storage. High-temperature short-time (HTST, 110 °C/8.6 s) treatment was used as a control. The results revealed a significantly greater presence of polyphenols, carotenoids, ascorbic acid content, and antioxidant capacity in tomato juice after HHP processing than after HTST processing. However, the total carotenoids and total phenolic content in HHP-treated tomato juice decreased dramatically and approached that in the HTST-treated tomato juice after 1 week of storage. Therefore, HHP's advantage in maintaining antioxidant compounds and capacity was only evident during the first week of storage in tomato juice. Nevertheless, the post-storage caffeic acid, quercetin, ferulic acid, and p-coumaric acid concentrations were 8.31, 4.77, 1.86, and 6.84 μg/g higher in the HHP-treated than in HTST-treated tomato juice, respectively. This study provides a new perspective for predicting HHP products' quality-stable shelf life.

Hydrostatic pressure

Pasteurization

Pascalization

10.48130/fia-2023-0016

Cite

Citations (14)

Construction and analyses of SSH cDNA libraries of a cucumber mutant of yellow virescent.

Xibei zhiwu xuebao (2010)

Juanjuan Li Chen FuLong Xin Li Lei Wang Jia JunZhong

The genes of differential expression between the mutant of yellow virescent and its wild type were separated used the suppressive subtraction hybridization(SSH) technique.We constructed two differential expressing cDNA libraries with the cDNAs of the mutant of yellow virescent as tester and the cDNAs of its wild type as driver,vice versa.One hundred and thirty three positive clones of forward SSH and seventy three positive clones of reverse SSH were identified and sequenced.We sequenced the clones expressing differentially in two SSH libraries and blasted their sequences.After that we submitted 159 ESTs which were not cucumber and there were no repeats,given the accession No.GH270133~GH270291.93 known function ESTs were classificated,the result showed that some ESTs were homology with the gene which regulate the porphyrin and chlorophyll metabolism,photosynthesis,signal transduction,transcription factors,amino acid metabolism,carbohydrate metabolism and lipid metabolism.

Suppression subtractive hybridization

Wild type

Homology

Source

Cite

Citations (0)

Cloning and transient expression analysis of isopentenyl diphosphate isomerase gene(IPI)promoter from Jatropha curcas

Sichuan Daxue Xuebao. Zirankexueban (2016)

Hu Xiaole Min Zhang Tian Yinshuai Ying Xu Fang Chen

Cloning (programming)

Jatropha curcas

Source

Cite

Citations (1)

Molecular cloning regulation of chilling--repressed gene {\sl atpA} in {\sl Elumus sibiricus}

PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS (2005)

Wenxing He Ying Xu Tang Lin Wei Qin Jing Li

Cloning (programming)

Source

Cite

Citations (0)

Cloning of a new aquaporin gene (JcPIP) from Jatropha curcas and analysis of its function under drought stress.

Redai yaredai zhiwu xuebao (2008)

Wang Yun-xiao Zhang Ying Luding Jiang Niu Bei Fang Chen

cDNA of Jatropha curcas was amplified by PCR with degenerated primers,a new plasma membrane intrinsic protein gene (JcPIP) was cloned. Phylogenic analysis indicated that PIPs of Jatropha curcas was closer relationship to those in Ricinus communis,Vitis vinifera and Spinacia oleracea. The cRNA of JcPIP was hetero expression in xenopus oocytes as a water channel and increased swelling rate by 10 times. A 13 amino acid sequence peptide was selected from the hydrophilic conserved peptides. Then a high titer polyclonal antibody was obtained by immunizing rabbits with the peptide. ELISA analysis showed the titer of rabbit serum against anti-peptide of JcPIP was up to 10-4. The location of JcPIP in Jatropha curcas was analysised with western-blot. JcPIP was major in 29 kDa band and located in all plant tissues. Treated with PEG-6000,the amount of JcPIP incresed,however,decreased under the conditon of rewater. It suggested that JcPIP related with drought tolerance of Jatropha curcas.

Jatropha curcas

Jatropha

Spinacia

Cloning (programming)

Osmotic shock

Source

Cite

Citations (2)

Functional Analysis of M-Locus Protein Kinase Revealed a Novel Regulatory Mechanism of Self-Incompatibility in Brassica napus L.

International Journal of Molecular Sciences (2019)

Fang Chen Yong Yang Bing Li Zhiquan Liu Fawad Khan

Self-incompatibility (SI) is a widespread mechanism in angiosperms that prevents inbreeding by rejecting self-pollen. However, the regulation of the SI response in Brassica napus is not well understood. Here, we report that the M-locus protein kinase (MLPK) BnaMLPKs, the functional homolog of BrMLPKs in Brassica rapa, controls SI in B. napus. We identified four paralogue MLPK genes in B. napus, including BnaA3.MLPK, BnaC3.MLPK, BnaA4.MLPK, and BnaC4.MLPK. Two transcripts of BnaA3.MLPK, BnaA3.MLPKf1 and BnaA3.MLPKf2, were generated by alternative splicing. Tissue expression pattern analysis demonstrated that BnaA3.MLPK, especially BnaA3.MLPKf2, is highly expressed in reproductive organs, particularly in stigmas. We subsequently created RNA-silencing lines and CRISPR/Cas9-induced quadruple mutants of BnaMLPKs in B. napus SI line S-70. Phenotypic analysis revealed that SI response is partially suppressed in RNA-silencing lines and is completely blocked in quadruple mutants. These results indicate the importance of BnaMLPKs in regulating the SI response of B. napus. We found that the expression of SI positive regulators S-locus receptor kinase (SRK) and Arm-Repeat Containing 1 (ARC1) are suppressed in bnmlpk mutant, whereas the self-compatibility (SC) element Glyoxalase I (GLO1) maintained a high expression level. Overall, our findings reveal a new regulatory mechanism of MLPK in the SI of B. napus.

10.3390/ijms20133303

Cite

Citations (39)

Imaging Lignin-Downregulated Alfalfa Using Coherent Anti-Stokes Raman Scattering Microscopy

BioEnergy Research (2010)

Yining Zeng Brian G. Saar Marcel G. Friedrich Fang Chen Yu-San Liu

Lignocellulosic Biomass

10.1007/s12155-010-9079-1

Cite

Citations (54)

Transcriptome Landscape of Mycobacterium smegmatis

Frontiers in Microbiology (2017)

Xin‐Feng Li Han Mei Fang Chen Qing Tang Zhaoqing Yu

The non-pathogenic bacterium Mycobacterium smegmatis mc2155 has been widely used as a model organism in mycobacterial research, yet a detailed study about its transcription landscape remains to be established. Here we report the transcriptome, expression profiles and transcriptional structures through growth-phase-dependent RNA sequencing (RNA-seq) as well as other related experiments. We found: (1) 2,139 transcriptional start sites (TSSs) in the genome-wide scale, of which eight samples were randomly selected and further verified by 5'-RACE; (2) 2,233 independent monocistronic or polycistronic mRNAs in the transcriptome within the operon/sub-operon structures which are classified into five groups; (3) 47.50% (1016/2139) genes were transcribed into leaderless mRNAs, with the TSSs of 41.3% (883/2139) mRNAs overlapping with the first base of the annotated start codon. Initial amino acids of MSMEG_4921 and MSMEG_6422 proteins were identified by Edman degradation, indicating the presence of distinctive widespread leaderless features in M. smegmatis mc2155. (4) 150 genes with potentially wrong structural annotation, of which 124 proposed genes have been corrected; (5) eight highly active promoters, with their activities further determined by β-galactosidase assays. These data integrated the transcriptional landscape to genome information of model organism mc2155 and lay a solid foundation for further works in Mycobacterium.

Mycobacterium smegmatis

Transcription

10.3389/fmicb.2017.02505

Cite

Citations (54)