Fei Zheng

Southeast University

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Jingjie Sha

Southeast University

Bin Yao

Chinese Academy of Agricultural Sciences

Xiangming Xie

Beijing Forestry University

Yunfei Chen

Eli Lilly (United States)

Huiying Luo

Chinese Academy of Agricultural Sciences

Huiying Luo

Chinese Academy of Agricultural Sciences

Shaojun Ding

Nanjing Forestry University

Tao Tu

Chinese Academy of Agricultural Sciences

Xiaoyu Wang

Beijing Forestry University

Yuan Wang

East China University of Science and Technology

Cooperative Institutions

Chinese Academy of Sciences

Southeast University

Nanjing Forestry University

Beijing Forestry University

Chinese Academy of Agricultural Sciences

Shanghai Jiao Tong University

Zhejiang University

University of Chinese Academy of Sciences

Chinese Academy of Medical Sciences & Peking Union Medical College

Peking University

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Detection of DNA translocations in a nanopore series circuit using a current clamp

Physical Chemistry Chemical Physics (2023)

Fei Zheng Yi Tao Wei Xu Jingjie Sha

Here we proposed a new strategy combining the nanopore series circuit and the current clamp to get the voltage trace across the nanopore, hence producing a voltage blockade signal when DNA translocate. This signal exhibits an intriguing charging and discharging phenomenon.

Nanopore

SIGNAL (programming language)

Clamp

10.1039/d2cp04530c

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Citations (8)

A nanostructured lateral flow immunoassay strip combined with Au@SiO₂ SERS nanotags for multiplex biomarker detection

Materials Advances (2023)

Geng Zhu Yuanbao Zhan Lu Yu Fei Zheng Yu Wan

A nanostructured lateral flow immunoassay strip with Au@SiO 2 SERS nanotags was constructed. This is the first time nanostructured T line and SERS-LFA has been combined, which has great potential for early non-invasive detection of AD biomarkers in blood.

Multiplex

10.1039/d3ma00595j

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Citations (3)

Engineering the Expression and Characterization of Two Novel Laccase Isoenzymes from Coprinus comatus in Pichia pastoris by Fusing an Additional Ten Amino Acids Tag at N-Terminus

PLoS ONE (2014)

Gu Chunjuan Fei Zheng Liangkun Long Jing Wang Shaojun Ding

The detail understanding of physiological/biochemical characteristics of individual laccase isoenzymes in fungi is necessary for fundamental and application purposes, but our knowledge is still limited for most of fungi due to difficult to express laccases heterologously. In this study, two novel laccase genes, named lac3 and lac4, encoding proteins of 547 and 532-amino acids preceded by 28 and 16-residue signal peptides, respectively, were cloned from the edible basidiomycete Coprinus comatus. They showed 70% identity but much lower homology with other fungal laccases at protein level (less than 58%). Two novel laccase isoenzymes were successfully expressed in Pichia pastoris by fusing an additional 10 amino acids (Thr-Pro-Phe-Pro-Pro-Phe-Asn-Thr-Asn-Ser) tag at N-terminus, and the volumetric activities could be dramatically enhanced from undetectable level to 689 and 1465 IU/l for Lac3 and Lac4, respectively. Both laccases possessed the lowest Km and highest kcat/Km value towards syringaldazine, followed by ABTS, guaiacol and 2,6-dimethylphenol similar as the low redox potential laccases from other microorganisms. Lac3 and Lac4 showed resistant to SDS, and retained 31.86% and 43.08% activity in the presence of 100 mM SDS, respectively. Lac3 exhibited higher decolorization efficiency than Lac4 for eleven out of thirteen different dyes, which may attribute to the relatively higher catalytic efficiency of Lac3 than Lac4 (in terms of kcat/Km) towards syringaldazine and ABTS. The mild synergistic decolorization by two laccases was observed for triphenylmethane dyes but not for anthraquinone and azo dyes.

ABTS

Enzyme Kinetics

Guaiacol

Coprinus

Volvariella volvacea

Pichia

10.1371/journal.pone.0093912

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Citations (52)

Improving the Catalytic Performance of a Talaromyces leycettanus α-Amylase by Changing the Linker Length

Journal of Agricultural and Food Chemistry (2017)

Duoduo Zhang Tao Tu Yuan Wang Yeqing Li Xue‐Gang Luo

A novel α-amylase, Amy13A, that consists of these domains was identified in Talaromyces leycettanus JCM12802: catalytic TIM-barrel fold, domain B, domain C, Thr/Ser-rich linker region, and C-terminal CBM20 domain. The wild type and three mutant enzymes were then expressed in Pichia pastoris GS115 to identify the roles of linker length (Amy13A21 and Amy13A33) and CBM20 (Amy13A-CBM) in catalysis. All enzymes had similar enzymatic properties, exhibiting optimal activities at pH 4.5-5.0 and 55-60 °C, but varied in catalytic performance. When using soluble starch as the substrate, Amy13A21 and Amy13A33 showed specific activities (926.3 and 537.8 units/mg, respectively, vs 252.1 units/mg) and catalytic efficiencies (kcat/Km, 25.7 and 22.0 mL s-1 mg-1, respectively, vs 15.4 mL s-1 mg-1) higher than those of the wild type, while Amy13A-CBM performed worse during catalysis. This study reveals the key roles of the CBM and linker length in the catalysis of GH13 α-amylase.

Linker

Enzyme Kinetics

10.1021/acs.jafc.7b00838

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Citations (16)

Modulation of Ion Transport in Nanopores Using Polyethylene Glycol

Langmuir (2024)

Fei Zheng Haiying Li Jun Yang Haiyan Wang Guangle Qin

Ion transport in nanopores is crucial for various biological and technological processes, exhibiting unique behaviors compared to bulk solutions. In this study, we systematically explore how polyethylene glycol (PEG) modulates ion transport within a conical nanopore. Our experiments reveal that introducing PEG into the ionic solution induces a reversal in ion current rectification (ICR). We further investigate the impact of PEG concentration, molecular weight, nanopore size, and cation type on ion transport. Additionally, we assess three different configurations of PEG introduction, identifying diffusive flow driven by an asymmetric cation distribution within the nanopore as a dominant transport mechanism. Our results confirm that the interactions between PEG and cations significantly affect ion transport properties. These findings advance our understanding of macromolecular crowding effects on ion transport and suggest potential applications in iontronic devices and biomolecule sensing.

Nanopore

Biomolecule

10.1021/acs.langmuir.4c03911

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Citations (1)

Table of Contents

American Journal Of Pathology (2022)

Dan Predescu Babak Mokhlesi Sanda Predescu Elizabeth S. Taglauer Elisha M. Wachman

Table (database)

10.1016/s0002-9440(22)00058-x

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Citations (0)

GLASS TRANSITION OF OXYGEN PLASMA TREATED ENZYMATIC HYDROLYSIS LIGNIN

BioResources (2012)

Xiaoyan Zhou Fei Zheng Xueyuan Liu Li‐Juan Tang Gi Xue

This study investigated the effect of oxygen plasma treatment on the glass transition temperature of enzymatic hydrolysis lignin (EHL) derived from the production of bio-ethanol.Differential scanning calorimetry (DSC) was used to obtain the glass transition temperature (T g ) of EHL.The results showed that the T g value of EHL under different heating rates ranged from 160 to 200 °C, and there was a strong linear correlation between heating rate and T g .The T g value of oxygen plasma treated EHL decreased when compared with the untreated samples.The apparent T g of the untreated sample was 168.2 °C, while the value of the treated sample was 161.5 °C.Distinct chain scission and introduction of oxygen-based functional groups on the surface of EHL were detected by XPS analysis.These changes may occur mainly on the bulky side chain and thus enhance molecular mobility of EHL.This indicates that oxygen plasma treatment can modify the structure and improve the reactivity of EHL efficiently.

Enzymatic Hydrolysis

10.15376/biores.7.4.4776-4785

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Citations (14)

Characterization of two thermophilic cellulases from Talaromyces leycettanus JCM12802 and their synergistic action on cellulose hydrolysis

PLoS ONE (2019)

Yuan Gu Fei Zheng Yuan Wang Xiaoyun Su Yingguo Bai

Talaromyces leycettanus JCM12802 is a great producer of thermophilic glycoside hydrolases (GHs). In this study, two cellulases (TlCel5A and TlCel6A) belonging to GH5 and GH6 respectively were expressed in Pichia pastoris and functionally characterized. The enzymes had acidic and thermophilic properties, showing optimal activities at pH 3.5-4.5 and 75-80°C, and retained stable at temperatures up to 60°C and over a broad pH range of 2.0-8.0. TlCel5A and TlCel6A acted against several cellulose substrates with varied activities (3,101.1 vs. 92.9 U/mg to barley β-glucan, 3,905.6 U/mg vs. 109.0 U/mg to lichenan, and 840.3 and 0.09 U/mg to CMC-Na). When using Avicel, phosphoric acid swollen cellulose (PASC) or steam-exploded corn straw (SECS) as the substrate, combination of TlCel5A and TlCel6A showed significant synergistic action, releasing more reduced sugars (1.08-2.87 mM) than the individual enzymes. These two cellulases may represent potential enzyme additives for the efficient biomass conversion and bioethanol production.

Enzymatic Hydrolysis

10.1371/journal.pone.0224803

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Citations (18)

Position control of ball and plate system based on switching mechanism

Fei Zheng Xiaoli Li Shangjun Wang Da‐Wei Ding

According to the dynamic characteristics of the ball and plate system, the mathematical model and the corresponding simplified model are derived, and the analysis of the applications of different types of PID controllers is given. Based on the result of the analysis about different controllers, the controller by using switching mechanism is proposed for position control of the ball and plate system. Finally, all these algorithms are tested on the GPB2001 ball and plate system experimental platform. The studying by experiment shows that the effect of switching control is better than conventional PID control.

Ball (mathematics)

Position (finance)

10.1109/ical.2011.6024719

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Citations (9)

A novel nitrogen-dependent gene associates with the lesion mimic trait in wheat

Theoretical and Applied Genetics (2016)

Lei Li Xuan Shi Fei Zheng Changcheng Li Di Wu

Common wheat

10.1007/s00122-016-2758-3

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Citations (16)