Jizhong Zhao

Huazhong University of Science and Technology

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Hongmei Hu

Jinggangshan University

Rui Wang

Huazhong University of Science and Technology

Jingwen Zhang

Huazhong University of Science and Technology

Sheng Wang

Huazhong University of Science and Technology

Li Wang

Huazhong University of Science and Technology

Ping Yu

First Hospital of China Medical University

Qianjin Liang

Institute of Genetics and Developmental Biology

Jianli Sang

Beijing Normal University

Yan Xu

First Affiliated Hospital of Zhengzhou University

Yueqing Wang

Beijing Normal University

Cooperative Institutions

Zhejiang Cancer Hospital

Nanjing Medical University

Chinese Academy of Sciences

University of Chinese Academy of Sciences

Sun Yat-sen University

Cancer Hospital of Chinese Academy of Medical Sciences

Huazhong University of Science and Technology

Central South University

Shanghai Jiao Tong University

Fudan University

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Labeling of polyprenylated macromolecules using mild Ene reactions

Tetrahedron (2021)

Jingwen Zhang Jizhong Zhao Li Wang Hongmei Hu Sheng Wang

Proteome

10.1016/j.tet.2020.131917

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Citations (12)

Reactive oxygen signaling molecule inducible regulation of CRISPR-Cas9 gene editing

Cell Biology and Toxicology (2022)

Jizhong Zhao Hongmei Hu Hongling Zhou Jingwen Zhang Li Wang

Guide RNA

Gene knockout

Gene targeting

10.1007/s10565-022-09723-3

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Citations (3)

MAGEA1 inhibits the expression of BORIS via increased promoter methylation

Journal of Cell Science (2018)

Jizhong Zhao Yueqing Wang Qianjin Liang Yan Xu Jianli Sang

Melanoma-associated antigen A1 (MAGEA1) and BORIS are members of the cancer testis antigens (CTA) family. Their functions and expression regulation mechanisms are not fully understood. In this study, we revealed new functions and regulatory mechanisms of MAGEA1 and BORIS in breast cancer cells, which were investigated in parental and genetically manipulated breast cancer cells via gene overexpression or siRNA interference-mediated down-regulation. We identified the interaction between MAGEA1 and CTCF, which was required for the binding of MAGEA1 to BORIS promoter and critical for the recruitment of DNMT3a. A protein complex containing MAGEA1, CTCF and DNMT3a will be formed before or after the conjunction with BORIS promoter. The binding of this complex to the BORIS promoter accounts for the hypermethylation and repression of BORIS expression, which results in cell death in the breast cancer cell lines tested. Multiple approaches are employed, including co-IP, GST-pull down, co-localization, cell death analyses using the Annexin V-FITC/PI double staining and caspase3 activation assays, ChIP and bisulfite sequencing PCR assays for methylation. These results have implications in the development of strategies in CTA-based immune therapeutics.

CTCF

Bisulfite sequencing

10.1242/jcs.218628

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Citations (12)