Novel magnetic imprinted polymers are designed by molecular simulation from a comprehensive perspective and prepared rationally by combining the modified surface imprinting technique with the improved atom transfer radical polymerization. The simulation results show that interaction intensity between indole and monomers are simultaneously affected by complex conformation, charge transfer and binding energy. The optimal monomer for the removal of indole is AM and the best ratio is 1 : 4 in the studied three monomers. To verify the reliability and accuracy of the simulation results, three kinds of novel magnetic imprinted polymers are prepared with different monomers. The experimental results show that molecular simulation is reliable in processing the pre-assembled complexes of molecularly imprinted polymers (MIPs). The MIPs using AM as monomer display the highest selectivity (2.561) and bonding percentage (31.062%). Structural superiority of the optimal adsorbent is reflected by using several characterization methods. A series of static adsorption tests such as kinetic, isotherm and selectivity are used to analyse the adsorption performance. The test results show that the novel adsorbents conform to the Elovich kinetic equation and follow the Langmuir isotherm model. Meanwhile, they display higher selectivity towards indole than towards other analogues. The novel adsorbents have potential application value in the denitrogenation field.
In this study, a novel and quick method for selective adsorption of dibenzothiophene (DBT) from gasoline using magnetic molecularly imprinted polymers (MMIPs) as the adsorbent has been employed. The MMIPs were prepared by a surface molecular imprinting technique, using Fe 3 O 4 magnetite nanoparticles as a magnetically susceptible component, DBT as template molecule, 4-vinylpyridine (4-VP) as functional monomer and ethylene glycol dimethacrylate (EGDMA) as cross-linker. The synthetic MMIPs were characterized by Fourier transform infrared (FT-IR) spectroscopy, scanning electron microscopy (SEM) and vibrating sample magnetometer (VSM). The adsorption performances of MMIPs were investigated by batch adsorption experiments in terms of adsorption kinetics, isotherms and selective recognition. The results of the study indicated that MMIPs were able to adsorb DBT well, the adsorption equilibrium time was about 5 h and the equilibrium adsorption amount was 22.23 mg/g at 318 K. In the corresponding selectivity test, MMIPs exhibited a greater adsorption capacity towards DBT than the other three analogues.
The data file name corresponds to the graph or image given in the paper. The file "Fig2d_1_FROG_RawData" contains a data cube of FROG measurements. In this cube, each column represents a delay step with an interval of 6.6 fs. Each row corresponds to a specific wavelength, and the wavelength data is provided in the file "Fig2d_2_lamb.txt". The files "Fig2f.sis, Fig3.sis, Fig5.sis, and Fig6.sis" with the .sis extension are hyperspectral image data cubes. Each data cube consists of 640 × 480 pixels and 1069 wavelength points. It is important to mention that all the .sis files provided are after retrieval data. Please note that the recorded wavelength in these files represents the wavelength of the sum frequency signal, and it is recorded as a value with a unit of 10-7 m. The file "Fig4.zip" contains individual images extracted from the high-speed imaging video. Each image corresponds to a frame, and the time interval between consecutive frames is 0.2 ms.
The laboratory experiment of photocatalysis coupled with Fenton reagent for degradation of p-nitrophenol(PNP) is intended to evaluate the influence of parameters including pH value,concentrations of initial PNP,Fe2+ and H2O2,as well as the amount of catalyst TiO2 added. It is reported that 100% degradation of p-nitrophenol is realized within 40 min,with UV illumination of 250W,additions of Fe2(+20mg/L) ,H2O2(5mmol/L) and TiO2(0.6g/L) . It is also found that coupled photocatalysis and Fenton reagent has led to a synergetic action;and GC/MS can help understand the intermediates produced during the degradation of PNP.
Abstract With the increasing use of plastic product, there is a large amount of plastic waste containing plasticizer, especially dibutyl phthalate (DBP), which would pollute the water and soil, and even cause diseases to humans and wildlife. Normally, the DBP in soil or water is trace, making it hard to be detected exactly. To solve this case, core‐shell structured molecularly imprinting polymers (P‐MIPs) were synthesized. Combing with photoresponsive monomer 4‐[(4‐methacryloyloxy) phenylazo] benzenesulfonic acid (MAPASA), the prepared P‐MIPs can be controlled by light to specifically absorb (440 nm or darkness condition) and release (365 nm) DBP, with the absorption capacity of 4.45 mg g −1 and imprinting factor of 3.56. More important, the P‐MIPs exhibit excellent reusability and practicability by applied to enrich and extract DBP in river water samples. In conclusion, the proposed P‐MIPs offer an idea for the detection of trace pollutants.
Introduction Senecavirus A (SVA), belonging to the genus Senecavirus in the family Picornaviridae , is an emerging pathogen causing vesicular disease in pigs. The main clinical manifestations of SVA infection include high mortality in neonatal piglets, skin ulceration, and vesicular lesions. So far, there is no commercially available vaccines or drugs against SVA. Construction of SVA infectious clones carrying reporter genes will help understand the characteristics of SVA and promote vaccine development. Methods In this study, we established a reverse genetics system for a local SVA isolate and used it to rescue a recombinant SVA, rSVA-eGFP, expressing the enhanced green fluorescent protein (eGFP) by inserting eGFP, GSG linker and the P2A sequence between 2A and 2B genes. Results We found that rSVA-eGFP exhibited a high replication efficiency comparable to the parental virus, was able to express the eGFP reporter efficiently and stable in maintaining the reporter gene up to six rounds of serial passages in BHK-21 cells. In mice, rSVA-eGFP also showed similar replication kinetics and pathogenicity to the parental virus, both causing mild lung lesions. In addition, a high-throughput viral neutralization assay was developed using eGFP as a surrogate readout in a fluorescence-based direct titration (FBT) assay based on rSVA-eGFP, facilitating rapid and accurate determination of the neutralizing antibody (nAb) titers. Discussion The successful establishment of an SVA reverse genetics system and the rescue of rSVA-eGFP would create a powerful tool for future studies of SVA replication mechanisms and pathogenicity as well as for antiviral development.
'/%3e%3cuse xlink:href='%23a' transform='rotate(23.036 .093 25.536)'/%3e%3cuse xlink:href='%23a' transform='rotate(45.87 1.273 16.18)'/%3e%3cuse xlink:href='%23a' transform='rotate(69.945 .996 12.078)'/%3e%3cuse xlink:href='%23a' transform='rotate(20.66 -19.689 31.932)'/%3e%3c/svg%3e)