Elisabeth Jiménez

Estación Experimental del Zaidín

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David R. Yáñez-Ruíz

Estación Experimental del Zaidín

Mercedes Montiel

Light Prescriptions Innovators (Spain)

Alejandro Belanche

Universidad de Zaragoza

A. I. Martín-García

Estación Experimental del Zaidín

Juan Manuel Palma-Hidalgo

Scotland's Rural College

M Morell

Hospital Clínico Universitario Virgen de la Victoria

Alfonso Clemente

Estación Experimental del Zaidín

M. Carmen Marín‐Manzano

Estación Experimental del Zaidín

JÁ

José Ángel Narváez

Universidad de Málaga

Santo Marsigliante

University of Salento

Cooperative Institutions

Hospital Universitario 12 De Octubre

Research Institute Hospital 12 de Octubre

Estación Experimental del Zaidín

Cambridge University Press

New York University Press

Consejo Superior de Investigaciones Científicas

Instituto de Salud Carlos III

Universidad Complutense de Madrid

Universidad de Málaga

Spanish National Cancer Research Centre

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Effect of thyroid hormones on angiotensinogen production in the rat in vivo and in vitro

Journal of Endocrinology (1987)

M. Ruiz Mercedes Montiel Elisabeth Jiménez M Morell

The influence of thyroid hormones on angiotensinogen production was studied in vitro and in vivo. In the in-vitro system, angiotensinogen production rate (APR) of monolayer cultures of rat hepatocytes in response to tri-iodothyronine (T3) and thyroxine (T4) was assayed. In the in-vivo system, plasma angiotensinogen concentration (PAC) and liver angiotensinogen content (LAC) were measured in hyper- and hypothyroid rats. In both thyroid dysfunctions, a significant decrease of PAC was found compared with that in control animals; however, LAC showed a significant increase in hyperthyroidism and a marked decrease in hypothyroidism. As PAC is dependent upon both angiotensinogen production by the liver and angiotensinogen degradation by renin, the decrease in PAC observed in hyperthyroidism could be due to an increase in plasma renin concentration, which would overcome the increased synthesis of liver angiotensinogen observed in these animals. In fact, addition of various concentrations of T4 or T3 to monolayer cultures of adult rat hepatocytes significantly enhanced APR. This increase was greater and started earlier with T3 (1196.1 +/- 143.7 (S.D.) pg/mg protein per 6-h incubation; significant differences at the third hour of incubation) than with T4 (858.3 +/- 88.2 pg/mg protein per 6-h incubation; significant differences at the sixth hour of incubation). In addition, a close dose-response relationship was found in the cultures supplemented with T3. The different time-course in the response elicited by T3 and T4 on APR could be a consequence of the necessary transformation of T4 into T3 to acquire biological activity.

Plasma renin activity

10.1677/joe.0.1150311

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Citations (22)

Hay diferencias en el uso de reglas ortográficas entre niños con y sin riesgo de presentar dificultades de aprendizaje en escritura

Grupo Editorial Universitario (GEU Editorial) eBooks (2013)

Elisabeth Jiménez Juan E. Jiménez Eduardo García Celia Morales Rando Christian Peake

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BJN volume 101 issue 7 Cover and Back matter

British Journal Of Nutrition (2009)

Michaël Gilsenan E. D. Bruin Louise Dye Carmina Wanden-Berghe Javier Sanz‐Valero

An abstract is not available for this content so a preview has been provided. As you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

Content (measure theory)

10.1017/s0007114509319352

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Angiotensin II (AII) and adrenal gland AII receptors in rats with congenital hypothyroidism

Journal of Endocrinology (1993)

M. J. Matilla Mercedes Montiel Elisabeth Jiménez

ABSTRACT Plasma angiotensin II (AII) concentration, plasma renin concentration (PRC), lung angiotensin-converting enzyme (ACE) activity and adrenal gland AII receptor isoforms have been evaluated in the postnatal development of the rat, in order to determine the alterations of the renin-angiotensin system (RAS) which occur in congenital hypothyroidism. The developmental AII profiles observed in control and hypothyroid rats were quite similar. Thus, AII was elevated at birth and declined at week 5 of life to reach adult values. However, AII levels were lower in hypothyroid than in euthyroid animals before their stabilization. On the other hand, PRC in newborn hypothyroid and euthyroid animals was higher than in adulthood, being significantly increased immediately after birth in hypothyroid rats. Pulmonary ACE activity in both experimental groups was low at birth and increased with age. This increase was greater in euthyroid rats than in congenitally hypothyroid animals from day 28 of life. These results confirm that plasma AII levels in rats are predominantly controlled by plasma renin activity, although other factors, such as renin substrate availability, may be responsible for the reduced plasma AII concentration in congenital hypothyroidism during the first weeks of life. The developmental profile of the adrenal gland AII receptor showed four isoforms, with pI values of 6·8, 6·7, 6·5 and 6·3. AII receptor-complex expression increased with age but, in congenitally hypothyroid rats, a higher induction of AII receptor isoforms migrating to pI 6·8, 6·5 and 6·3 was observed. These findings show that thyroid hormone deficiency in early life can have an important role in the postnatal induction of the RAS components, which may be essential for the regulation of blood pressure, and for fluid and electrolyte balance in developing rats. Journal of Endocrinology (1993) 137, 231–238

Plasma renin activity

10.1677/joe.0.1370231

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Comparison of sampling by rumen cannula or stomach tube in sheep and goats: fermentation parameters and rumen microorganisms.

XV Jornadas sobre Producción Animal, Zaragoza 14 y 15 de mayo de 2013. (2013)

E. Ramos‐Morales A. I. Martín-García Elisabeth Jiménez A. Arco-Pérez David R. Yáñez-Ruíz

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The cytotoxic effects of Bowman-Birk proteins from legumes on colon cancer cells are related to their ability to inhibit serine proteases

Alfonso Clemente M. Carmen Marín‐Manzano Elisabeth Jiménez Gabriella Sonnante F. Javier Moreno

Resumen del trabajo presentado al 5th International Food Legumes Research Conference (IFLRC V) y al 7th European Conference on Grain Legumes celebrados en Antalya (Turquia) del 26 al 30 de abril de 2010.

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Renin-angiotensin system in hypothyroid rats: effects of potassium iodide and triiodo-L-thyronine

European Journal of Endocrinology (1984)

Elisabeth Jiménez Mercedes Montiel José Ángel Narváez M Morell

Abstract. Kinetic studies of the renin-angiotensin system (RAS) were carried out by measuring plasma renin activity (PRA), plasma renin concentration (PRC) and plasma renin substrate (PRS). Changes in this system were studied during hypothyroidism, after administration of propylthiouracil (PTU), and in thyroidectomized rats. A significant decrease in PRA and PRC was observed in those animals previously treated with PTU. However, a significant increase in PRC, and a decrease in PRS, were found in T animals, but no changes in PRA were observed. In these animals, after daily administration of potassium iodide for I week (T+KI), no changes in RAS were observed in comparison with T rats. Nevertheless, administration of daily doses of triiodo- i thyronine (T+T 3 ) induced a significant increase in PRA, leaving PRC unaltered. In this case the changes in PRA were related to the increase in PRS after T 3 treatment. These results suggest that two different mechanisms were involved in renin release, one activated in T rats and the other in pharmacological hypothyroidism.

Thyronine

10.1530/acta.0.1050505

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Potassium-induced contraction in the lamb proximal urethra: involvement of norepinephrine and different calcium entry pathways.

Journal of Pharmacology and Experimental Therapeutics (1990)

Ángeles García-Pascual Gonzalo Costa Dolores Isla Elisabeth Jiménez Ana García-Sacristán

The purpose of this work was to investigate the mechanisms involved in the peculiar biphasic response of the lamb urethral smooth muscle to high K+ solutions. The relative amplitude of the phasic and tonic components of the contraction and its reproducibility were dependent on the concentration of K+ used. Only concentrations higher than 80 mM (i.e., 120 mM) showed a tonic component greater in amplitude than the phasic one and manifested a tachyphylactic effect. Phentolamine (10(-6) M), prazosin (10(-6) M) and chemical denervation with 6-hydroxydopamine significantly inhibited the tonic component of the K+ (120 mM)-induced contraction, modifying its morphology. Reproducible contractions to K+ (120 mM) could be obtained in the presence of prazosin (10(-6) M) or cocaine (10(-6) M). The preparations were also shown to accumulate [3H]noradrenaline and release it upon depolarization with K+ (60 and 120 mM). Calcium removal inhibited the K+ (120 mM)-induced contraction. After addition of calcium (0.5-5 mM) the contractile activity was restored. Nifedipine (10(-6) M) and verapamil (10(-6) M) but not sodium nitroprusside (10(-6) M) significantly blocked the contractile response for calcium as well as the phasic component of the K+ contraction in calcium-containing medium. In preparations treated with prazosin (10(-6) M) the tonic component of the K+ (120 mM) contraction was more sensitive to nifedipine and removal of extracellular calcium than the phasic one.(ABSTRACT TRUNCATED AT 250 WORDS)

Tonic (physiology)

Phentolamine

10.1016/s0022-3565(25)22940-8

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The anti-proliferative effect of TI1B, a major Bowman–Birk isoinhibitor from pea (Pisum sativumL.), on HT29 colon cancer cells is mediated through protease inhibition

British Journal Of Nutrition (2012)

Alfonso Clemente M. Carmen Marín‐Manzano Elisabeth Jiménez María del Carmen Arques Claire Domoney

Bowman–Birk inhibitors (BBI) from legumes, such as soyabean, pea, lentil and chickpea, are naturally occurring plant protease inhibitors which have potential health-promoting properties within the mammalian gastrointestinal tract. BBI can survive both acidic conditions and the action of proteolytic enzymes within the stomach and small intestine, permitting significant amounts to reach the large intestine in active form to exert their reported anti-carcinogenic and anti-inflammatory properties. In a previous study, we reported the ability of a recombinant form of TI1B (rTI1B), representing a major BBI isoinhibitor from pea, to influence negatively the growth of human colorectal adenocarcinoma HT29 cells in vitro. In the present study, we investigate if this effect is related directly to the intrinsic ability of BBI to inhibit serine proteases. rTI1B and a novel engineered mutant, having amino acid substitutions at the P 1 positions in the two inhibitory domains, were expressed in the yeast Pichia pastoris . The rTI1B proved to be active against trypsin and chymotrypsin, showing K i values at nanomolar concentrations, whereas the related mutant protein was inactive against both serine proteases. The proliferation of HT29 colon cancer cells was significantly affected by rTI1B in a dose-dependent manner (IC 50 = 31 ( sd 7) μ m ), whereas the inactive mutant did not show any significant effect on colon cancer cell growth. In addition, neither recombinant protein affected the growth of non-malignant colonic fibroblast CCD-18Co cells. These findings suggest that serine proteases should be considered as important targets in investigating the potential chemopreventive role of BBI during the early stages of colorectal carcinogenesis.

10.1017/s000711451200075x

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Citations (67)

Evaluation of the nutritional value and presence of minerals and pesticides residues in agro-industrial by-products to replace conventional ingredients of small ruminant diets

Small Ruminant Research (2023)

Mahmoud Hassan Alejandro Belanche Elisabeth Jiménez Inés Rivelli A. I. Martín-García

Silage

Melon

Pomace

10.1016/j.smallrumres.2023.107117

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Citations (2)