To reduce pain after total hip replacement (THR), researchers are interested in drug-free interventions. However, there is still a lack of consensus on their prevention efficacy. We performed a meta-analysis to evaluate the use of nonpharmaceutical interventions for postoperative pain management after THR. We searched the Cochrane Library, MEDLINE, EMBASE, Web of Science, PEDRO, and ClinicalTrials.gov databases for articles published between and 1991 and 2020. The main outcome measures were postoperative pain, opioid consumption, and quality of life (QoL). In total, 1,942 patients were studied. We found moderate evidence indicating postoperative pain relief measured by the Western Ontario and McMaster Universities Arthritis Index Scale, with mean differences (MDs) of -0.28 (95% confidence interval [CI], -0.49 to -0.07; P=0.01; I2 =0%) within three months, -0.19 (95% CI, -0.40 to 0.02; P=0.07; I2 =0%) between 3-6 months, and -0.13 (95% CI, -0.35 to 0.08; P=0.21; I2 =0%) between 6-12 months. Additionally, we found that acupuncture therapy could reduce opioid analgesic consumption (MD, -0.98; 95% CI, -1.18 to -0.79; fentanyl [mg/h]; P<0.01; I2 =72.2%) and significantly improve pain relief with an MD of 0.90 (95% CI, 0.47 to 1.33; P<0.01; I2 =0%) measured using the visual analog scale. Electrotherapy slightly improved perceived pain with an MD of 0.22 (95% CI, -0.27 to 0.70; P=0.37; I2 =0%). Moreover, moderate evidence has shown that preoperative exercises improve QoL. This meta-analysis suggested that continuous passive motion did not improve pain or QoL. Postoperative exercise was associated with pain relief and improved QoL. Acupuncture therapy after THR has been shown to reduce opioid analgesic consumption.
Abstract Objectives Our present study has shown a potential role for VEGF‐A‐mediated autocrine signalling to promote survival and proliferation of SU‐DHL‐6 cells, but the cells could not undergo apoptosis but rather decrease proliferation after bevacizumab treatment. Therefore, we would like to further study the antitumour efficacy of venetoclax (BCL2 inhibitor) in combination with bevacizumab in B‐cell NHL. Methods The human cytokine antibody array, RT‐qPCR, Western blot, ELISA, apoptosis assay and xenografted mouse model et al were used. Results We described a unique phenomenon that SU‐DHL‐6 cells showed cell density‐dependent survival and growth. Then, we suggested the expression of VEGF‐A was positively correlated with the cell density using a human cytokine antibody array and indicated an important role of VEGF‐A in the survival and proliferation of SU‐DHL‐6 cells. Additionally, xenografted SU‐DHL‐6 cells formed tumours in mice that grew in response to VEGF stimulation. GEO data set also suggested that high VEGF‐A expression reflected poor prognosis. The combination therapy with bevacizumab and navitoclax could significantly induce of cell death in vitro and reduce the tumour size and weight with well tolerated in vivo. Conclusions Our findings propose a novel combined strategy in which bevacizumab synergises with the BCL2 inhibitor venetoclax that is effective against B‐cell NHL.
Abstract Background: A large number of randomized controlled trials (RCTs) have shown that acupuncture (ACU) has certain advantages over pharmacotherapies in the treatment of primary osteoporosis (POP). However, due to the diversity of ACU treatments, its relative effectiveness have not yet been studied and explained. Therefore, based on the network meta-analysis (NMA), this study will compare the differences in the efficacy of multiacupuncture in the treatment of POP, to provide a reference for clinical treatment. Methods: We will search PubMed, MEDLINE, Embase, the Cochrane Library, China National Knowledge Infrastructure (CHKD-CNKI), WANFANG database (Chinese Medicine Premier), Chinese Biomedical Literature database (CBM), and VIP for relevant RCTs of ACU treatments for POP, from their inceptions to January 2019. STATA 15.0 and GEMTC software will be used to perform a NMA. The evidence will be evaluated by the Grading of Recommendations Assessment, Development, and Evaluation (GRADE) approach and the type 1 error rate will be assessed by trial sequential analysis (TSA). Results: The results of this review will be submitted to a recognized journal for publication. Conclusion: This proposed systematic review will evaluate the different advantages of various types of ACU in the treatment of POP. Registration: PROSPERO (registration number CRD42019122724).
Background and Aims: Aberrant transcriptional programs are highly regulated processes that play important roles in the development and progression of hepatocellular carcinoma (HCC). Emerging evidence suggests that super-enhancers (SEs) often drive critical oncogene expression. However, SE-associated genes in HCC pathogenesis are still poorly understood. Methods: We performed integrative ChIP-seq and Hi-C analyses of HCC cells and identified ajuba LIM protein (AJUBA) as a SE-associated gene. We evaluated AJUBA expression in HCC using immunohistochemistry, immunoblotting, and qRT-PCR. ChIP and luciferase reporter assays were performed to demonstrate that transcription factor 4 (TCF4) bound to AJUBA-associated SEs. We then assessed the role of AJUBA in HCC using both in vitro and in vivo assays. Epithelial-mesenchymal transition (EMT) was examined using immunofluorescence and immunoblotting assays. Furthermore, we used immunoprecipitation and BiFC assays to explore the underlying mechanisms. Results: We identified AJUBA as a SE-associated oncogene in HCC regulated by TCF4. High AJUBA expression was related to an aggressive phenotype and unfavorable outcome in HCC patients. AJUBA knockdown significantly reduced cell migration and invasion capacities both in vitro and in vivo. Furthermore, AJUBA overexpression in HCC recruited tumor necrosis factor associated factor 6 (TRAF6), enhancing the phosphorylation of Akt and increasing Akt activity toward GSK-3β, thus promoting EMT. Conclusions: Our results provide functional and mechanistic links between the SE-associated gene AJUBA and tumor EMT in aggressive HCC.
Abstract The unbalanced mass of the rotor-bearing system may cause abnormal vibration of the rotor-bearing system, which may affect the safe operation of the system in severe cases. In this paper, the rotor-bearing system fault simulation test bench and MADYN 2000 were used to test and simulate the single-disk shafting rotor mass unbalance fault. The research analyzes the influence of speed, unbalanced mass, support stiffness, and phase of unbalanced mass on the vibration response of the rotor-bearing system. The results show that when the speed is higher than the first-order critical speed, after the rotor starts, the amplitude gradually increases with the speed, reaches the peak at the first-order critical speed, and then gradually decreases to a stable value. Due to the anisotropy of the bearing support, the rotor has different amplitudes in each direction, but the change trend is the same, the support stiffness is small, and the stable value is reached faster. When the rotation speed and the unbalanced mass phase are the same, the rotor amplitude and the rotor unbalanced mass have a linear relationship. When the speed and unbalanced mass are the same, the rotor amplitude fluctuates with the change of the loading phase, and the fluctuation period is π. The research results have certain reference significance for the fault diagnosis and dynamic characteristics analysis of the unbalanced response of the rotor-bearing system.
At present, reservoir reconstruction technology is a possible and practical way to solve the commercial exploitation problem of hydrates, such as low production, limited production range, and high production cost. The research mechanism of hydraulic fracturing in NGH (natural gas hydrate) reservoirs has not formed a theoretical system for the most common reservoir reconstruction technology. In this work, according to the measured geological conditions of site GMGS3-W19, a multi-cluster hydraulic fracturing model for horizontal wells in NGH reservoirs is established with the extended finite element method. The effects of fracture spacing, hydrate saturation, and horizontal in situ geo-stress difference on the final propagation path of multi-cluster fractures are analyzed. When the alternating fracturing and sequential fracturing are adopted, the subsequent fracture will have a certain extrusion force on the formed fractures, which makes the existing fractures continue to propagate and produce a second expansion. The total lengths of fractures generated by alternating and sequential fracturing modes for values of fracture spacing from 8 to 20 m are longer than that of simultaneous fracturing mode. The hydrate saturation increases from 20 to 60%, and the fracture propagation path hardly changes. However, the fracture propagation of HBS (hydrate-bearing-sediments) with high hydrate saturation is hindered, which leads to a small fracture length in alternating fracturing and simultaneous fracturing modes. When sequential fracturing is adopted, the interference between fractures of HBS with low hydrate saturation is apparent, and the bending degrees of hydraulic fractures are large. When the hydrate saturation reaches 60%, the last fracture will hardly bend and expand along the perforation direction. The difference of horizontal geo-stress increases, and the interference between fractures is more substantial, specifically reflected in the increase of the second fracture expansion lengths and further expansion along the direction of the maximum horizontal geo-stress.
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