Jie Gao

Soochow University

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Ailin Luo

Huazhong University of Science and Technology

Hui Ruan

Nanjing Forestry University

Fengying Gu

State Key Laboratory of Food Science and Technology

Guoqing He

Zhejiang University

Chaoliang Tang

University of Science and Technology of China

Qihe Chen

Zhejiang University

Jiawu Wang

Chongqing Medical University

Yinhua Gong

Soochow University

Chun Yang

Capital Medical University

Yilin Zhao

Jilin Agricultural University

Cooperative Institutions

Zhejiang University

Huazhong University of Science and Technology

Soochow University

Nanjing Medical University

Chinese Academy of Sciences

Chinese Academy of Medical Sciences & Peking Union Medical College

Shanghai Jiao Tong University

University of Science and Technology of China

First Affiliated Hospital of Soochow University

Anhui Medical University

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Palmatine Protects against Cerebral Ischemia/Reperfusion Injury by Activation of the AMPK/Nrf2 Pathway

Oxidative Medicine and Cellular Longevity (2021)

Chaoliang Tang Junmou Hong Chengyun Hu Chunxia Huang Jie Gao

Palmatine (PAL), a natural isoquinoline alkaloid, possesses extensive biological and pharmaceutical activities, including antioxidative stress, anti‐inflammatory, antitumor, neuroprotective, and gastroprotective activities. However, it is unknown whether PAL has a protective effect against ischemic stroke and cerebral ischemia/reperfusion (I/R) injury. In the present study, a transient middle cerebral artery occlusion (MCAO) mouse model was used to mimic ischemic stroke and cerebral I/R injury in mice. Our study demonstrated that PAL treatment ameliorated cerebral I/R injury by decreasing infarct volume, neurological scores, and brain water content. PAL administration attenuated oxidative stress, the inflammatory response, and neuronal apoptosis in mice after cerebral I/R injury. In addition, PAL treatment also decreases hypoxia and reperfusion‐ (H/R‐) induced neuronal injury by reducing oxidative stress, the inflammatory response, and neuronal apoptosis. Moreover, the neuroprotective effects of PAL were associated with the activation of the AMP‐activated protein kinase (AMPK)/nuclear factor E2‐related factor 2 (Nrf2) pathway, and Nrf2 knockdown offsets PAL‐mediated antioxidative stress and anti‐inflammatory effects. Therefore, our results suggest that PAL may be a novel treatment strategy for ischemic stroke and cerebral I/R injury.

10.1155/2021/6660193

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Genome-Wide Identification, Expression Patterns, and Functional Analysis of UDP Glycosyltransferase Family in Peach (Prunus persica L. Batsch)

Frontiers in Plant Science (2017)

Boping Wu Liuxiao Gao Jie Gao Yaying Xu Hongru Liu

Peach (Prunus persica L. Batsch) is a commercial grown fruit trees, important because of its essential nutrients and flavor promoting secondary metabolites.The glycosylation processes mediated by UDP-glycosyltransferases (UGTs) play an important role in regulating secondary metabolites availability. Identification and characterization of peach UGTs is therefore a research priority. A total of 168 peach UGT genes that distributed unevenly across chromosomes were identified based on their conserved PSPG motifs. Phylogenetic analysis of these genes with plant UGTs clustered them into 16 groups (A-P). Comparison of the patterns of intron-extron and their positions within genes revealed one highly conserved intron insertion event in peach UGTs. Tissue specificity, temporal expression patterns in peach fruit during development and ripening, and in response to abiotic stress UV-B irradiation was investigated using RNA-seq strategy. The relationship between UGTs transcript levels and concentrations of glycosylated volatiles was examined to select candidates for functional analysis. Heterologous expressing these candidate genes in E.coli identified UGTs that were involved in the in vitro volatile glycosylation. Our results provide an important source for the identification of functional UGT genes to potential manipulate secondary biosynthesis in peach.

Heterologous expression

10.3389/fpls.2017.00389

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Metagenome analysis of bacterial diversity in Tibetan kefir grains

European Food Research and Technology (2013)

Jie Gao Fengying Gu Jie He Jianzhong Xiao Qihe Chen

Weissella

Lactococcus

Shewanella

Diversity index

10.1007/s00217-013-1912-2

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Citations (56)

Serum Levels of MMP-8 and MMP-9 as Markers in Chronic Subdural Hematoma

Journal of Clinical Medicine (2022)

Gaojian Su Jie Gao Chu-wei Wu Junfeng Zou Di Zhang

Chronic subdural hematoma (CSDH) is a common neurological disease that involves the collection of blood products in the subdural space. The progression of CSDH is an angiogenic and inflammatory process, but the multifactorial mechanisms underlying CSDH are still not fully understood. We aimed to identify one or more factors that may play an important role in the development of CSDH. We enrolled 83 patients with CSDH, including 17 postoperative patients, and analyzed 20 markers in the hematoma fluid and peripheral blood of each patient. Overall differential gene expression was examined to identify the representative markers. The concentration of MMP-8 was significantly lower in the postoperative group than in the preoperative group. The concentration of MMP-9 was significantly higher in the postoperative group than in the preoperative group. These findings indicate that MMP-8 and MMP-9 may play important roles in the pathophysiology of CSDH. Understanding the pathways associated with CSDH may provide insights for improving disease outcomes.

Chronic Subdural Hematoma

Pathophysiology

10.3390/jcm11040902

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Quantitative assessment methods and qualitative evaluation system for the complementary characteristics of multi-energy

Journal of Environmental Management (2024)

Yichao Xu Zhiqiang Jiang Zenghai Zhao Fangliang Zhu Jie Gao

10.1016/j.jenvman.2024.122142

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The simultaneous quantification of Ganoderma acids and alcohols using ultra high-performance liquid chromatography–mass spectrometry in dynamic selected reaction monitoring mode

Journal of Pharmaceutical and Biomedical Analysis (2012)

Jie Gao Naoto Sato Masao Hattori Chao‐Mei Ma

Terpene

Sample Preparation

10.1016/j.jpba.2012.11.003

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Citations (7)

Two ω-3 FADs Are Associated with Peach Fruit Volatile Formation

International Journal of Molecular Sciences (2016)

Jiaojiao Wang Hong-Ru Liu Jie Gao Yuji Huang Bo Zhang

Aroma-related volatiles, together with sugars and acids, play an important role in determining fruit flavor quality. Characteristic volatiles of peach fruit are mainly derived from fatty acids such as linoleic acid (18:2) and linolenic acid (18:3). In the present study, six genes encoding fatty acid desaturases (FAD) were cloned, including two ω-6 FAD genes (PpFAD2, PpFAD6) and four ω-3 FAD genes (PpFAD3-1, PpFAD3-2, PpFAD7 and PpFAD8). Heterologous expression of peach FADs in tobacco plants showed that PpFAD3-1, and PpFAD3-2 significantly reduced contents of 18:2, and accumulated significant higher levels of 18:3. In the case of volatiles, transgenic plants produced lower concentrations of hexanal and higher levels of (E)-2-hexenal. Consequently, the ratio of the (E)-2-hexenal and hexanal was about 5- and 3-fold higher than that of wild type (WT) in PpFAD3-1 and PpFAD3-2 transformants, respectively. No significant changes in volatile profiles were observed in transgenic plants overexpressing the four other peach FAD genes. Real-time quantitative polymerase chain reaction (qPCR) analysis showed that ripe fruit had high PpFAD3-1 and low PpFAD3-2 transcript levels. In contrast, high PpFAD3-2 and low PpFAD3-1 transcript levels were observed in young fruit. These results indicate a temporal regulation of these two ω-3 FADs during development and ripening, influencing peach fruit volatile formation.

Linolenic acid

10.3390/ijms17040464

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Citations (27)

Lattice-based mesoscale simulations and mean-field theory of cell membrane adhesion

Methods in enzymology on CD-ROM/Methods in enzymology (2024)

Long Li Jie Gao Łukasz Milewski Jinglei Hu Bartosz Różycki

Multiscale Modeling

Lattice (music)

10.1016/bs.mie.2024.03.005

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Citations (1)

Mitochondrial DNA induces nucleus pulposus cell pyroptosis via the TLR9-NF-κB-NLRP3 axis

Journal of Translational Medicine (2023)

Lü Peng Huayong Zheng Hao Meng Chuan Liu Lianhong Duan

Nucleus pulposus cell (NPC) death and progressive reduction play important roles in intervertebral disc degeneration (IVDD). As part of a damage-associated molecular pattern, mitochondrial DNA (mtDNA) can be recognized by TLR9 and triggers the expression of NF-κB and NLRP3 inflammasomes, inducing pyroptosis and inflammatory response. However, whether mtDNA induces NPC pyroptosis via the TLR9-NF-κB-NLRP3 axis and promotes IVDD remains uncertain.We constructed an in vitro NPC oxidative stress injury model to clarify the mechanism of mtDNA release, TLR9-NF-κB signaling pathway activation, and NPC injury. We further verified the mechanism of action underlying the inhibition of mtDNA release or TLR9 activation in NPC injury in vitro. We then constructed a rat punctured IVDD model to understand the mechanism inhibiting mtDNA release and TLR9 activation in IVDD.We used human NP specimen assays to show that the expression levels of TLR9, NF-κB, and NLRP3 inflammasomes correlated with the degree of IVDD. We demonstrated that mtDNA mediated TLR9-NF-κB-NLRP3 axis activation in oxidative stress-induced human NPC pyroptosis in vitro. Oxidative stress can damage the mitochondria of NPCs, causing the opening of the mitochondrial permeability transition pores (mPTP) and leading to the release of mtDNA into the cytosol. Furthermore, inhibition of mPTP opening or TLR9 activation blocked TLR9-NF-κB-NLRP3 axis activation and thereby mediated NPC pyroptosis and IVDD.mtDNA plays a key role in mediating NPC pyroptosis and IVDD via the TLR9-NF-κB-NLRP3 axis. Our findings provide new potential targets for IVDD.

Pyroptosis

TLR9

10.1186/s12967-023-04266-5

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Citations (26)

Clinical Analysis of 40 Cases with Severe Drug Eruption Caused by Carbamazepine

Zhongguo Pifu Xingbing Xue Zazhi (2016)

Shi Jian Jie Gao Bao Jianan Miao Liyan

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