Meifeng Li

β-Lactoglobulin (βLg) conjugated with epigallocatechin-3-gallate (EGCG) to form βLg-EGCG conjugates by covalent bonding under alkaline conditions. This conjugation resulted in structural and activity changes, providing a possible approach for allergen desensitization, but with low desensitization efficiency. This study aimed to use ultrasound to improve the conjugation rate of βLg and EGCG and investigate changes in the binding mechanism and functional properties. Scanning electron microscopy revealed βLg-EGCG conjugates to be in the ceoss-linked state, however, it also highlighted fragments of various forms with ultrasonic treatment. Ultrasonic treatment increased the binding affinities (Ka) from 2.5×10 5 M -1 to 6.3×10 5 M -1 and 1.9×10 5 M -1 to 3.0×10 5 M -1 at 4℃ and 44 ℃, respectively. Moreover, ultrasound treatment increased secondary structural changes in βLg in the conjugated state, accompanied by an enhanced inhibition of immunoglobulin E (IgE) attachment and acquisition of antioxidant properties. Ultrasound induced an increase in hydrodynamic diameter and lowered the absolute zeta potential of βLg-EGCG conjugates, resulting in a decrease in thermal stability and an increase in digestibility, as well as foaming and emulsification abilities, improving the application of EGCG conjugation in dairy products.

Gallate

10.2139/ssrn.4126897

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One Body with Two Sides:The Relationship between Education and Technology from a Technical View of Humanism

Meifeng Li

Currently,there is an absence of technical view in the theory of education under the multi-views,Which induced to a certain extent the hard condition of the dialog between pedagogy and educational technology.Actually,education has a same quality with technology in the technical view of humanism.Furthermore,education and technology influence each other and turn into a dialectic unity,so these two become two sides of one body.We think this relationship may be the base of the dialog between pedagogy and educational technology.

Dialectic

Technology education

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Molecular characterization of the β-lactoglobulin conjugated with fluorescein isothiocyanate: Binding sites and structure changes as function of pH

International Journal of Biological Macromolecules (2019)

Xiaoning Zhang Yacine Hémar Lei Lv Tao Zhao Yuhong Yang

Fluorescein isothiocyanate

Conjugate

Zeta potential

10.1016/j.ijbiomac.2019.08.181

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