Pseudobagrus fui Miao is a valid bagrid species that can be separated from all its congeners in having 27–33 anal-fin rays. Among the species of Pseudobagrus, it forms part of a group of approximately 20 species putatively characterized by having a smooth anterior margin of the pectoral-fin spine and short maxillary barbels not extending to the base of the pectoral-fin spine. Pseudobagrus fui, together with P. nitidus and P. vachelli, can be differentiated from all other forktailed species of this group by having no fewer than 20 branched anal-fin rays, the posterior end of the anal-fin base posterior to the vertical through the posterior end of the adipose-fin base, and anal-fin base longer than the adipose-fin base. It further differs from P. nitidus in having a gas bladder without beaded lateral and posterior margins, and from P. vachelli in having short maxillary barbels not extending to the base of the pectoral-fin spine. Pseudobagrus fui is currently known from the main stream of the upper Yangtze River and its tributaries, the Min River, Jialing River, Tuo River, Wu River and Chishui River in Sichuan Province, Guizhou Province, and the Chongqing City. The identity and nomenclature of Pseudobagrus nitidus is also discussed.
Abstract A new method to label fucoidan sulfate was established with tyramine and fluorescein isothiocyanate isomer I (FITC). Fluorescence spectrophotometry and high performance liquid chromatography verified the successful labelling of fucoidan by FITC. The results of the single‐pass intestinal perfusion indicated that the jejunum and ileum are the main absorption sites, and there was carrier saturation. In addition, fucoidan sulfate at 1 mg/ml had no inhibitory effect on Caco‐2 cell proliferation. Studies on the transmembrane transport mechanism showed that fucoidan can be absorbed because the apparent permeability coefficient of the drugs (P app ) A → B was 3.78 + 0.03 ×10 −6 and that of B → A was 1.42 + 0.19 ×10 −6 . The peak absorption of fucoidan occurred at 120 min after administration; moreover, the higher the concentration used, the worse the absorption was, suggesting the saturation of transport carriers. The absorption was temperature dependent: the absorption at 37°C was much better than that at 4°C. Further, the absorption of fucoidan sulfate might rely on clathrin endocytosis as chlorpromazine (10 μg/ml) significantly inhibited it.
Triplophysa lixianensis, a new nemacheiline loach species, is described from the Min Jiang of the upper Yangtze River drainage in Sichuan Province, South China. It can be separated from all other species of Triplophysa by having a unique combination of the following characters: posterior chamber of gas bladder greatly reduced or absent; caudal peduncle columnar with a roughly round cross-section at its beginning; anterior edge of lower jaw completely exposed or uncovered by lower lip; intestine short, forming a zigzag loop below stomach; dorsal-fin origin closer to caudal-fin base than to snout tip; pelvic fin inserted anterior to dorsal-fin origin; snout length 50.6–57.5 % of head length; eye diameter 12.3– 15.4 % of head length; caudal peduncle length 25.1–27.1 % of standard length; anal fin with five branched rays; lower lip greatly furrowed with two thick lateral lobes; and body smooth or scaleless.
In the rapid development of molecular biology, nucleic acid amplification detection technology has received more and more attention. The traditional polymerase chain reaction (PCR) instrument has poor refrigeration performance during its transition from a high temperature to a low temperature in the temperature cycle, resulting in a longer PCR amplification cycle. Peltier element equipped with both heating and cooling functions was used, while the robust adaptive fuzzy proportional integral derivative (PID) algorithm was also utilized as the fundamental temperature control mechanism. The heating and cooling functions were switched through the state machine mode, and the PCR temperature control module was designed to achieve rapid temperature change. Cycle temperature test results showed that the fuzzy PID control algorithm was used to accurately control the temperature and achieve rapid temperature rise and fall (average rising speed = 11 °C/s, average falling speed = 8 °C/s) while preventing temperature overcharging, maintaining temperature stability, and achieving ultra-fast PCR amplification processes (45 temperature cycle time < 19 min). The quantitative results show that different amounts of fluorescence signals can be observed according to the different concentrations of added viral particles, and an analytical detection limit (LoD) as low as 10 copies per μL can be achieved with no false positive in the negative control. The results show that the TEC amplification of nucleic acid has a high detection rate, sensitivity, and stability. This study intended to solve the problem where the existing thermal cycle temperature control technology finds it difficult to meet various new development requirements, such as the rapid, efficient, and miniaturization of PCR.
This dataset contains the digitized treatments in Plazi based on the original journal article Zamani-Faradonbe, Mazaher, Zhang, E., Keivany, Yazdan (2021): Garra hormuzensis, a new species from the upper Kol River drainage in the Persian Gulf basin (Teleostei: Cyprinidae). Zootaxa 5052 (3): 380-394, DOI: https://doi.org/10.11646/zootaxa.5052.3.4
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Red-necked pond turtle ( Mauremys nigricans )
PHOTO: FLPA/ALAMY STOCK PHOTO
A recent Report by Z. Ouyang et al. (“Improvements in ecosystem services from investments in natural capital,” 17 June, p. [1455][2]) found that investment in the restoration and preservation of
Onychostoma brevibarba, a new cyprinid species, is described from two tributaries flowing into the Xiang Jiang (= River) of the middle Chang Jiang basin in Hunan Province, South China. The new species is morphologically similar to two Chinese congeners, O. minnanense and O. barbatulum, but differs from them in the anteromedian extension of the postlabial groove. It further differs from O. minnanense in the maxillary-barbel length, shape and body coloration, and from O. barbatulum in the number of lateral-line perforated scales and the width of the mouth opening. The validity of the new species and its close relationship with these two species were affirmed by a molecular phylogenetic analysis based on the mitochondrial cyt b and CO1 genes.
A new cyprinid species from the He Jiang of the Zhu Jiang River(or Pearl River)basin in Guangxi,China is described here.Parasinilabeo longibarbus sp.nov.Holotype:IHB 2002051387,49.6 mm SL,from the He Jiang flowing to the Zhu Jiang River basin in Fuchuan County,Guangxi,China;collected by Lan Jia-Hu in April,2002.Paratypes:IHB 2002051405—7,2002051413,2002051410,2002051424—5,2001060469,8 specimens,43.9—53.3 mm,same data as holotype.Count and morphometric data for the type material:D,iv,8;A,iv.5;P,i,12—13;V,i,8.Pharyngeal teeth in three rows,3.4.5—5.4.3;Perforated lateral line scales 37—40;scales above lateral line 5—6 and below 4.5—5;predorsal scales 14—17;circumpeduncular scales 16.Body depth 24.1—30.6(average 26.8)% SL,head length 22.0—24.8(23.7),length of caudal peduncle14.1—17.6(15.6),depth of caudal peduncle 9.5—12.7(11.2),dorsal-fin length 17.8—23.2(19.9),pectoral-fin length 14.2—18.7(16.5),pelvic-fin length 13.6—18.2(15.4),anal-fin length 15.0—19.1(16.7),predorsal length 50.6—53.3(52.4),prepectoral length 21.8—24.8(23.4),prepectoral length 52.8—57.7(55.7),and preanal length 74.1—79.9(77.7).Snout length 37.9—44.3(average 41.1)%HL,eye diameter 19.8—31.0(23.2),and interorbital space 46.5—53.1(49.2).Diagnosis:P.longibarbus were distinguished from all other congeners by possessing a pair of well-developed maxillary barbels,scales on the breast and belly embedded beneath the skin,and a postlabial groove extended lateromedially to divide the lower lip into two lateral lobes and one central part.The type specimens are deposited in the Museum of Aquatic Organisms at the Institute of Hydrobiology,Chinese Academy of Sciences,Wuhan 430072,Hubei Province,China.
Garra hormuzensis, new species, is described from the Kol River drainage. It is distinguished from its congeners in the Iranian part of the Persian Gulf basin by having 7 branched dorsal-fin rays, usually 9+8 branched caudal-fin rays, the breast, belly and back in front of the dorsal-fin origin covered by scales, and a free lateral and posterior margins of the gular disc. It is further characterised by having a minimum K2P distance of 1.16% to G. mondica in the mtDNA COI barcode region.
Most morphological characters diagnostic of the 13 Chinese species of the cyprinid genus Sinilabeo Rendahl, 1932, are identical to those of the genus Bangana Hamilton, 1822. Consequently, these 13 species are transferred to Bangana. A revised diagnosis is provided for the now-expanded genus Bangana, and a dichotomous key and taxonomic and nomenclatural notes are included for the following valid Chinese species: B. decora, B. dero, B. devdevi, B. discognathoides, B. lemassoni, B. lippa, B. rendahli, B. tonkinensis, B. tungting, B. wui, B. xanthogenys, B. yunnanensis, and B. zhui. Literature reports, by Chinese authors, of Sinilabeo dero from the upper Irrawady River basin, in Yunnan, are based on misidentifcations of B. devdevi. Sinilabeo cirrhinoides Wu and Lin in Wu, Lin, Chen, Chen and He, 1977, and S. laticeps Wu and Lin in Wu, Lin, Chen, Chen and He, 1977, are junior subjective synonyms of B. devdevi and B. lippa, respectively. Sinilabeo yunnanensis Wu, Lin, Chen, Chen and He, 1977, is an available name, and a lectotype is designated for the species. Bangana zhui (Zheng and Chen, 1983) is a valid species distinct from B. yunnanensis.