Background: Observational studies on the association between subclinical hypothyroidism (SCH) and metabolic syndrome (MetS) have produced inconsistent results. Therefore, we performed a meta-analysis to evaluate the effect of SCH on the risk of MetS. Methods: Multiple databases were searched to identify studies on the association between SCH and the risk of MetS, up to February 2015. Relevant information for analysis was extracted. A random-effects model was used to calculate the pooled risk estimates. Results: 9 studies (7 cross-sectional and 2 case-control studies) were included. The pooled odds ratio (OR) for MetS comparing SCH with euthyroid subjects was 1.31 (95%CI: 1.08 to 1.60, p = 0.006, I2 = 50%). Subgroup analyses by countries revealed a significant association for the studies from Asian (OR = 1.244, 95% CI: 1.030–1.503, I2 = 25%) other than non-Asian (OR = 1.548, 95% CI: 0.925–2.591, I2 = 73.5%) countries. A positive association was identified in the IDF subgroup (OR = 1.288, 95% CI: 1.055–1.572, I2 = 0%), but not in the NCEP-ATP III (OR = 1.351, 95% CI: 0.950–1.923, I2 = 66.4%), Chinese (OR = 1.430, 95% CI: 0.891–2.294) and Japanese (OR = 1.542, 95% CI: 0.594–4.005, I2 = 78.3%) subgroup. A certain degree of heterogeneity was observed among studies which cannot be explained by study design, diagnostic criteria and location. Conclusion: Our results demonstrated that SCH was significantly associated with a higher risk of MetS. Well-designed cohort studies were warranted to confirm our findings.
Objective Observe the injectable salvia miltiorrhiza polyphenols acid salt Tongxinluo capsule treatment of unstable angina pectoris curative effect.Methods 110 cases of UAP patients were randomly divided into the treatment group and control group each 55 cases.The control group gives aspirin,simvastatin and nitroglycerin conventional treatment.In treatment group based on conventional treatment combined with the injectable salvia miltiorrhiza polyphenols acid salt intravenous drip and with Tongxinluo capsule.Two groups of course are 14 days.To compare two treatments for clinical curative effect and Anginal attacks,and record the adverse reaction.Results Treatment group total clinical curative effect for 87.3%,the control group was 70.9%,it is statistically significant(P 0.05).Conclusion Based on the routine therapy combined with the injectable salvia miltiorrhiza polyphenols acid salt and Tongxinluo capsule treatment of unstable angina pectoris symptoms,safety,better than conventional fight angina treatment.
Objective To discuss the clinical safety,effect and the prevention of complications of stent-assisted angioplasty in the treatment of symptomatic intracranial vertebrobasilar artery stenosis.Methods Totally 104 symptomatic intracranial vertebrobasilar artery stenosis patients diagnosed with ultrasonography and cerebral angiography underwent stent angioplasty.The incidences of perioperative and postoperative complications were analyzed.Results Intraoperative severe vasospasm induced the failure of operation in 1 patient.The technical success rate of treatment was 99.04%(103/104).The average preoperative stenosis rate was 82.23% and postoperative stenosis rate was less than 20.00%.The symptoms and neurological signs improved at different degree within 1 week after operation.Descent of heart rate happened in 24 patients 3 days after operation,and blood pressure decreased in 20 of them.One patient died of cerebral hemorrhage on 9th day after operation.Acute thrombosis occurred in 1 patient on 10th day after operation.At a follow-up of 2—36 months,2 patients died.The others remained symptom-free,among them TCD showed normal in 82,and DSA found no abnormality of stents in 12 patients.Conclusion Carotid artery stenting is a safe and effective method for symptomatic carotid and intracranial arterial stenosis.Risks during and after operations can be reduced with strict indications,skillful operation and regularly medicine taking.
The present study aimed to investigate the role of D4F, an apolipoprotein A-I mimetic peptide, in macrophage apoptosis induced by the glycated high-density lipoprotein (gly-HDL)-induced endoplasmic reticulum (ER) stress C/EBP homologous protein (CHOP) pathway, and unravel the regulatory role of autophagy in this process. Our results revealed that except for suppressing the accumulation of lipids within RAW264.7 macrophages caused by gly-HDL, D4F inhibited gly-HDL-induced decrease in the cell viability and increase in lactate dehydrogenase leakage and cell apoptosis, which were similar to 4-phenylbutyric acid (PBA, an ER stress inhibitor). Besides, similar to PBA, D4F inhibited gly-HDL-induced ER stress response activation evaluated through the decreased PERK and eIF2α phosphorylation, together with reduced ATF6 nuclear translocation as well as the downregulation of GRP78 and CHOP. Interestingly, D4F facilitated gly-HDL-triggered activation of autophagy, measured as elevated levels of beclin-1, LC3-II, and ATG5 expressions in macrophages. Furthermore, the inhibition effect of D4F on gly-HDL-induced ER stress-CHOP-induced apoptosis of macrophages was restrained after beclin-1 siRNA and 3-methyladenine (3-MA, an inhibitor of autophagy) treatments, while this effect was further reinforced after rapamycin (Rapa, an inducer of autophagy) treatment. Furthermore, administering D4F or Rapa to T2DM mice upregulated LC3-II and attenuated CHOP expression, cell apoptosis, and atherosclerotic lesions. However, the opposite results were obtained when 3-MA was administered to these mice. These results support that D4F effectively protects macrophages against gly-HDL-induced ER stress-CHOP-mediated apoptosis by promoting autophagy.
An entry from the Cambridge Structural Database, the world’s repository for small molecule crystal structures. The entry contains experimental data from a crystal diffraction study. The deposited dataset for this entry is freely available from the CCDC and typically includes 3D coordinates, cell parameters, space group, experimental conditions and quality measures.
Polymorphisms of the vitamin D receptor (VDR) gene may be a risk factor for pancreatic cancer (PC). We investigated the association of two single-nucleotide polymorphisms (SNPs) of the VDR gene with PC in age- and gender-matched patients and controls. PC (n=91) and healthy control (n=80) samples were genotyped for the FokI (rs2228570) and BsmI (rs1544410) polymorphisms using the PCR and restriction fragment length polymorphism (PCR-RFLP) method. Chi-square analysis was used to test for the overall association of VDR genotype with disease. There was a significant difference in the frequency of genotype FF between the PC patients and controls (Ptrend=0.009); however, the difference in frequency of genotype BB between the two groups was not significant (Ptrend=0.082). The difference between FF and Ff/ff frequency was significant (P=0.002). The two high-risk genotypes were ffbb and Ffbb, with an 11.66- and 6.42-fold increased risk of PC, respectively. VDR gene polymorphisms were important for the development of PC in this study population; however, further exploration of these findings and their implications are required.
Irisin is a newly discovered myokine which can relieve metabolic disorders and resist atherosclerosis. The effects of irisin on ox-LDL-induced macrophage apoptosis and endoplasmic reticulum stress-related pathways were observed in vitro. RAW264.7 macrophages were cultured in vitro and pretreated with irisin at 20, 40 and 80 ng/ml for 30 min, followed by culture with 100 mg/L ox-LDL and 5 mg/L tunicamycin (TM) for 12 h. The cell viability and apoptosis were detected by MTT assay and annexin V-FITC double staining. The nuclear translocation of activating transcription factor 6 (ATF6) was detected by immunofluorescence assay. Western blot was used to detect the expressions of p-PERK, p-eIF2α, C/EBP homologous protein (CHOP) and Bcl-2. Irisin reduced lipid accumulation in macrophages in a concentration-dependent pattern and significantly inhibited apoptosis induced by ox-LDL and TM. Compared with ox-LDL and TM groups, the expressions of CHOP, p-PERK and p-eIF2α in the irisin group significantly decreased, the translocation of ATF6 from cytoplasm to nucleus was significantly weakened, and Bcl-2 expression significantly increased. Irisin can alleviate the apoptosis of macrophages induced by ox-LDL, which may be achieved by inhibiting the PERK/eIF2α/CHOP and ATF6/CHOP endoplasmic reticulum stress signaling pathways.
Reaction of Fe(CO)2(NO)2 and [(ON)Fe(S,S-C6H3R)2]- (R = H (1), CH3 (1-Me))/[(ON)Fe(SO2,S-C6H4)(S,S-C6H4)]- (4) in THF afforded the diiron thiolate/sulfinate nitrosyl complexes [(ON)Fe(S,S-C6H3R)2 Fe(NO)2]- (R = H (2), CH3 (2-Me)) and [(ON)Fe(S,SO2-C6H4)(S,S-C6H4)Fe(NO)2]- (3), respectively. The average N-O bond lengths ([Fe(NO)2] unit) of 1.167(3) and 1.162(4) A in complexes 2 and 3 are consistent with the average N-O bond length of 1.165 A observed in the other structurally characterized dinitrosyl iron complexes with an {Fe(NO)2}9 core. The lower nu(15NO) value (1682 cm(-1) (KBr)) of the [(15NO)FeS4] fragment of [(15NO)Fe(S,S-C6H3CH3)2 Fe(NO)2]- (2-Me-15N), compared to that of [(15NO)Fe(S,S-C6H3CH3)2]- (1-Me-15N) (1727 cm(-1) (KBr)), implicates the electron transfer from {Fe(NO)2}10 Fe(CO)2(NO)2 to complex 1-Me/1 may occur in the process of formation of complex 2-Me/2. Then, the electronic structures of the [(NO)FeS4] and [S2Fe(NO)2] cores of complexes 2, 2-Me, and 3 were best assigned according to the Feltham-Enemark notation as the {Fe(NO)}7-{Fe(NO)2}9 coupling (antiferromagnetic interaction with a J value of -182 cm(-1) for complex 2) to account for the absence of paramagnetism (SQUID) and the EPR signal. On the basis of Fe-N(O) and N-O bond distances, the dinitrosyliron {L2Fe(NO)2} derivatives having an Fe-N(O) distance of approximately 1.670 A and a N-O distance of approximately 1.165 A are best assigned as {Fe(NO)2}9 electronic structures, whereas the Fe-N(O) distance of approximately 1.650 A and N-O distance of approximately 1.190 A probably imply an {Fe(NO)2}10 electronic structure.
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