Xiaolin Xie

China Pharmaceutical University

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Yinping Li

Wuhan University

Sujuan Liu

Tianjin Medical University

Xiaohua Fu

First Affiliated Hospital of Soochow University

Xianyu Li

Yan'an University

Cong Li

Hunan Normal University

Gaojing Qu

Evidence Based Research (United States)

Lei Tian

Shaanxi University of Science and Technology

Yun Chen

Peking University

Liping Xiang

Second Affiliated Hospital of Hunan University of Traditional Chinese Medicine

Chengyuan Liang

Shaanxi University of Science and Technology

Cooperative Institutions

Chinese Academy of Sciences

Huazhong University of Science and Technology

Sun Yat-sen University

Wuhan University

Central South University

Chongqing Medical University

Hunan Normal University

Shaanxi University of Science and Technology

Capital Medical University

Chinese Academy of Medical Sciences & Peking Union Medical College

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Rat BMSC infusion was unable to ameliorate inflammatory injuries in tissues of mice with LPS-induced endotoxemia

Bio-Medical Materials and Engineering (2017)

Xiaolin Xie Hanhan Liu Jinhua Wu Yun Chen Zhui Yu

Background:Mesenchymal stem cells (MSC) have been shown to have potent immunoregulatory effects. They are able to mitigate inflammation in many contexts of immune disorders, including autoimmune diseases and graft-versus-host disease (GVHD). Endotoxemia can induce systematic inflammation in the bod y. In this study, we try to investigate whether MSC can attenuate inflammation in models of LPS-induced endotoxemia. Methods:Bone marrow MSC (BMSC) were isolated and expanded from rats of 4~6-week age. Adult mice were divided randomly into Control group, Model group and BMSC group. LPS were injected peritoneally into mice of Model group and BMSC group to induce endotoxemia. For BMSC group mice, 1×106 BMSC were injected through tail vein 1 hour after LPS application. Animals were sacrificed after 24 hours. Inflammatory damage in lungs and livers were detected through histochemistry. Wet/dry ratio of lung tissues was calculated, levels of inflammatory factors as IL-1β and TNF-α in lung tissues were measured through ELISA. Results:Inflammatory pathological changes in lung and liver in BMSC group were comparable to those in Model group. Moreover, in some animals, the injuries were exacerbated after BMSC treatment. Accordingly, wet/dry ratio of lung in BMSC group mice was higher than that in Model group mice. IL-1β level in BMSC-treated group mice was significantly augmented, however, no significant changes were detected between these two groups for level of TNF-α. Conclusion:Our results showed that application of BMSC in LPS-induced endotoxemia models couldn't attenuate the inflammatory injuries in tissues. Although BMSC have been shown to be able to induce immune inhibition, however, in some instances, their immuno-inhibitory function might be regulated by the local environment.

10.3233/bme-171634

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Genistein promotes M1 macrophage apoptosis and reduces inflammatory response by disrupting miR-21/TIPE2 pathway

Saudi Pharmaceutical Journal (2022)

Cong Li Xiaolin Xie Sujuan Liu Liping Xiang Xiaohua Fu

Cardiovascular diseases are a major cause of mortality, and vascular injury, a common pathological basis of cardiovascular disease, is deeply correlated with macrophage apoptosis and inflammatory response. Genistein, a type of phytoestrogen, exerts cardiovascular protective activities, but the underlying mechanism has not been fully elucidated. In this study, RAW264.7 cells were treated with genistein, lipopolysaccharide (LPS), nuclear factor-kappa B (NF-κB) inhibitor, and/or protein kinase B (AKT) agonist to determine the role of genistein in apoptosis and inflammation in LPS-stimulated cells. Simultaneously, high fat diet-fed C57BL/6 mice were administered genistein to evaluate the function of genistein on LPS-induced cardiovascular injury mouse model. Here, we demonstrated that LPS obviously increased apoptosis resistance and inflammatory response of macrophages by promoting miR-21 expression, and miR-21 downregulated tumor necrosis factor-α-induced protein 8-like 2 (TIPE2) expression by targeting the coding region. Genistein reduced miR-21 expression by inhibiting NF-κB, then blocked toll-like receptor 4 (TLR4) pathway and AKT phosphorylation dependent on TIPE2, resulting in inhibition of LPS. Our research suggests that miR-21/TIPE2 pathway is involved in M1 macrophage apoptosis and inflammatory response, and genistein inhibits the progression of LPS-induced cardiovascular injury at the epigenetic level via regulating the promoter region of Vmp1 by NF-κB.

10.1016/j.jsps.2022.05.009

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Septic Inflammatory Microenvironment Induces Senescence of Bone Marrow Mesenchymal Stem Cells

Biomedical and health research (2019)

Xiaolin Xie Xianyu Li Bin Luo Zan Tong Qu Gaojing

Senescence

10.3233/bhr190033

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Development of vericiguat: The first soluble guanylate cyclase (sGC) stimulator launched for heart failure with reduced ejection fraction (HFrEF)

Biomedicine & Pharmacotherapy (2022)

Juan Xia Hui Nan Lei Tian Chengyuan Liang Jie Zhang

In recent years, with improvements in treatments for heart failure (HF), the survival period of patients has been extended. However, the emergence of some patients with repeated hospitalizations due to their worsening conditions and low survival rates followed. Currently, few drugs are available for such patients. Vericiguat was first drug approved for the treatment of symptomatic patients with chronic HF with reduced ejection fraction (HFrEF) to reduce the occurrence of worsening HF. This article provides comprehensive information about vericiguat in terms of drug design and development, structure-activity relationship (SAR), synthesis, pharmacological efficacy, and clinical practice. In addition, insights into the current vericiguat trials and treatments of HF are also discussed.

Drug Development

10.1016/j.biopha.2022.112894

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Citations (20)

Effects and mechanism of Qingke Pingchuan granules against influenza virus infection

Archives of Virology (2024)

Linqing He Jiarui Cao Xiaolin Xie Yayun Zhang Xue Zhang

Medical microbiology

10.1007/s00705-024-06053-z

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Citations (2)

Visual Assay for Methicillin-Resistant Staphylococcus aureus Based on Rolling Circular Amplification Triggering G-Quadruplex/Hemin DNAzyme Proximity Assembly

Analytical Chemistry (2023)

Xiaolin Xie Xiaoxue Cheng Juan Dong Jia Li Lanxin Jiang

Nowadays, infections caused by methicillin-resistant Staphylococcus aureus (MRSA) have constituted a new challenge for anti-infective treatment. Precise identification and rapid clinical diagnostics of MRSA from other methicillin-sensitive strains entail assays with robust diagnostic efficiency and simple operation steps. Sensitive detection of MecA gene is promising to indicate MRSA infection, but it is challenged by the lack of isothermal and simple strategies. A visual assay based on isothermal rolling circular amplification and G-quadruplex/hemin (G4/hemin) DNAzyme proximity assembly was proposed for the immediate, efficient, and cost-effective detection of MecA in simple operation steps and in a single tube. The presence of MecA specifically drove the formation of circular templates, which further triggered isothermal amplification. The amplified product offered abundant binding sites for DNA-grafted hemin probes to form a novel proximity-assembled G4/hemin DNAzyme structure for colorimetric changing diagnosis. This tandem-repeated novel DNAzyme possessed higher catalytic activity and a lower background signal than traditional G4/hemin DNAzyme, ensuring sensitive discrimination of MRSA (limit of detection: 9.6 pM). Assay stability and antimatrix interference capability enable clinical application, which shows compared diagnostic ability with classic methods (100% sensitivity and 100% specificity) but possesses more simplified procedures and shorter turnaround time (<6 h). This colorimetric strategy in a nonsite-specific and hypersensitive manner holds foreseeable prospects in clinical diagnostic and research applications.

Hemin

Deoxyribozyme

G-quadruplex

10.1021/acs.analchem.2c05712

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Citations (17)

Effects of inflammatory microenvironment on senescence of bone marrow mesenchymal stem cells and its mechanisms

Journal of Molecular and Cellular Cardiology (2020)

Xiaolin Xie Yinping Li

Senescence

HES1

Proinflammatory cytokine

10.1016/j.yjmcc.2019.11.073

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7-Difluoromethoxy-5,4′-dimethoxy-genistein attenuates macrophages apoptosis to promote plaque stability via TIPE2/TLR4 axis in high fat diet-fed ApoE mice

International Immunopharmacology (2021)

Cong Li Xiaolin Xie Sujuan Liu Liping Xiang Yong Zhang

Lysophosphatidylcholine

Apolipoprotein E

Knockout mouse

10.1016/j.intimp.2021.107477

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Citations (6)

One-time relieving of frozen shoulder motor dysfunction with pure acupotomy: A case report

Medicine (2023)

Kangyan Zhou Xiaolin Xie Jie Liu Jing Tao Qiong Liu

Frozen shoulder (FS) is characterized by shoulder pain and restricted movement of the shoulder joint. While it tends to resolve on its own, it significantly affects an individual quality of daily life. The pure acupotomy technique employs needle-knife manipulation as the sole treatment, without the use of medications, such as steroids or vitamins, and local anesthesia if necessary. It aims to restore soft tissue mechanical balance and circulation through techniques such as cutting and stripping, creating a "gap effect." This technique can rapidly, safely, and effectively relieve functional impairments in patients with FS. This article presents a case study of the successful treatment of FS using a purely needle-knife technique.

Frozen shoulder

10.1097/md.0000000000036783

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Citations (1)

Numerical optimization of flow noises for mufflers based on the improved BP neural network

Journal of Vibroengineering (2016)

Xiaolin Xie Feng Gao Xiaoyun Huang Chuan Huang Jie Li

Aimed at the large noise of tail pipe, the method of fluid dynamics was firstly applied to analyze the inner flow field of the exhaust muffler. According to the result, the large noise of tail pipe was mainly caused by air flow regeneration noise, and the vice muffler was not the major component for generating airflow noise. The largest pressure of the whole muffler system was at the outlet end of main mufflers. The largest flow velocity was in the connection pipe between main mufflers and vice mufflers. Secondly, boundary element model of transmission loss for the muffler was established to compare and analyze it with the experimental. The experimental and computational value of transmission loss for the muffler has a good consistency in both change trend and numerical value, and the computational model was reliable. Finally, GA-BP neural network algorithm was used to optimize the acoustic performance of the muffler. Airflow noises of the tail pipe were effectively reduced through optimizing the inner structure of the muffler.

Muffler

Transmission loss

Expansion chamber

10.21595/jve.2016.16671

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Citations (4)