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A simple and rapid method was proposed for the detection of amylaseproducing microorganisms on solid media using soluble starch as substrate. The soluble starch was incorporated into nutrient agar and amylase production could be detected when the color of the white media became grey around the colonies. The method was nondestructive, and allowed direct visualization and isolation of amylolytic microorganisms from the environment without prior replication of colonies. From the relationship of d with lnA,the amylase activity at pH 4.2 and pH 6.0 was determined. The acidtolerance ability could be easily testified by comparing amylase activity at pH 4.2 and pH 6.0,at starch agar plate.
Abstract Objectives To study the characteristics and prognosis of dermatomyositis (DM) with different initial symptoms. Patients and methods: A retrospective analysis was performed on the patients who were first diagnosed with DM from 1 Jan. 2019 to 1 Jan. 2021. According to different initial symptoms, patients were divided into five groups, including rash, myasthenia, arthritis, respiratory symptom and atypical symptom group. Clinical and laboratory data were recorded. All patients were followed up until 31st May 2021. Results In total 136 patients, rash (40%) was the most common initial symptoms of DM, followed by respiratory symptoms (22%), arthritis (20%), muscle weakness (10%) and atypical symptoms (8%). Rash groups and atypical groups had a higher positive rate of anti-TIF1γ antibodies than arthritis groups and respiratory symptom groups ( P < 0.05). Respiratory symptom and arthritis groups had a higher positive rate of anti-Ro52 antibodies than rash and myasthenia groups ( P < 0.05). Respiratory groups had a higher incidence of ILD than rash and atypical groups. The FVC and DLCO in respiratory group were significantly lower than rash, arthritis and atypical groups ( P < 0.05). The 3-year survival rate of rash groups was significantly higher than myasthenia groups and arthritis groups ( P < 0.05). Conclusions DM patients with different initial manifestations had different pulmonary function tests, myositis antibodies and prognosis.
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