Srinivasan Sivasubramanian

King Institute of Preventive Medicine and Research

Author Statistics

Papers

Citation

H-Index

i-10 index

Research Trends

Author Order

Document Type

Co-Authors

Ramar Thangam

Korea University

Palani Gunasekaran

King Institute of Preventive Medicine and Research

Thirumurugan Ramasamy

Bharathidasan University

Soundarapandian Kannan

Periyar University

Raju Vivek

Bharathiar University

Suresh Veeraperumal

Shantou University

M Deepika

Periyar University

Savariar Vincent

Uttarakhand State Council for Science and Technology

Kunka Mohanram Ramkumar

SRM Institute of Science and Technology

Arun Sridhar

Gandhi Medical College

Cooperative Institutions

King Institute of Preventive Medicine and Research

University of Madras

Central Leather Research Institute

Bharathidasan University

Bharathiar University

Council of Scientific and Industrial Research

Anna University, Chennai

Creative Commons

SRM Institute of Science and Technology

Periyar University

Author Statistics

Papers

Citation

H-Index

i-10 index

Research Field

International Journal of Pharmaceutical Sciences and Research (2016)

Poongodi Arasu Nalini Ganesan Srinivasan Sivasubramanian Palani Gunasekaran

Antimicrobial peptides (AMP) showed a broad spectrum of cytotoxic activity against cancer cells, which could provide a new class of anticancer drugs.In this study, we have evaluated the cytotoxic and apoptotic activity of magainin II, an AMP compared with 5-Fluorouracil (5-FU) in colo320 DM cancer cells, and magainin II cytotoxicity was assessed on vero cell line by MTT.The cells cultured in the presence or absence of magainin II and 5-FU.The magainin II inhibited cell viability in dose dependent manner was quantified by MTT assay.The IC 50 of magainin II was found to be 89.3nM and at this concentration the cytotoxicity was not observed in vero cell line.The cytotoxicity of the magainin II was further confirmed by lactate dehydrogenase (LDH) assay and neutral red uptake assay (NRU).Apoptosis induction was verified by DNA fragmentation analysis.The morphological changes in colo 320 DM cells was measured by DAPI and acridine orange/ethidium bromide staining, showed nuclear condensation, and this substantiates the apoptotic action of magainin II.The cell cycle analysis by FACS revealed that there was a accumulation of cells at G0/G1 phase in magainin II treated cells.Overall, these results revealed that magainin II has selective cytotoxicity in cancer cells and has the potential to induce apoptosis.Magainin II may offer a novel and effective therapeutic candidate in the treatment of colon cancer with potentially low cytotoxic effects on normal cells.These findings highlight the importance of magainin II as a drug capable of exerting an in vitro antitumoral activity by triggering apoptosis. INTRODUCTION:Antimicrobial peptides (AMP) gain increasing attention as potential candidates in cancer therapy and have various advantages such as selective cytotoxicity against cancer cells, active against multidrug-resistance mechanism as well as effects in combination therapy 1 .

10.13040/ijpsr.0975-8232.7(7).2951-58

Cite

Citations (0)

Feeding Solutions

Medical radiology (2021)

Srinivasan Sivasubramanian Karthiraj Natarajan Krishna Mohan Gummala

Jejunostomy

Enteral administration

Percutaneous endoscopic gastrostomy

Interventional radiology

Feeding tube

10.1007/978-3-030-65463-4_6

Cite

Citations (0)

Correction to HER2 Targeted Breast Cancer Therapy with Switchable “Off/On” Multifunctional “Smart” Magnetic Polymer Core–Shell Nanocomposites

ACS Applied Materials & Interfaces (2016)

Raju Vivek Ramar Thangam Selvaraj Rajesh Kumar Chandrababu Rejeeth Gopal Kumar

ADVERTISEMENT RETURN TO ISSUEPREVAddition/CorrectionNEXTORIGINAL ARTICLEThis notice is a correctionCorrection to HER2 Targeted Breast Cancer Therapy with Switchable "Off/On" Multifunctional "Smart" Magnetic Polymer Core–Shell NanocompositesRaju Vivek, Ramar Thangam, Selvaraj Rajesh Kumar, Chandrababa Rejeeth, Gopal Senthil Kumar, Srinivasan Sivasubramanian, Savariar Vincent, Dhanaraj Gopi, and Soundarapandian KannanCite this: ACS Appl. Mater. Interfaces 2016, 8, 15, 10048Publication Date (Web):April 6, 2016Publication History Published online6 April 2016Published inissue 20 April 2016https://pubs.acs.org/doi/10.1021/acsami.6b01968https://doi.org/10.1021/acsami.6b01968correctionACS PublicationsCopyright © 2016 American Chemical Society. This publication is available under these Terms of Use. Request reuse permissions This publication is free to access through this site. Learn MoreArticle Views746Altmetric-Citations2LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail PDF (103 KB) Get e-Alertsclose Get e-Alerts

10.1021/acsami.6b01968

Cite

Citations (3)

Combined effect of a natural flavonoid rutin from Citrus sinensis and conventional antibiotic gentamicin on Pseudomonas aeruginosa biofilm formation

Food Control (2018)

M Deepika Ramar Thangam Periasamy Sakthidhasan Arun Sridhar Srinivasan Sivasubramanian

Phytochemical

10.1016/j.foodcont.2018.02.044

Cite

Citations (79)

Serum levels of matrix metalloproteinases as prognostic markers for severe dengue with plasma leakage

Experimental and Molecular Pathology (2022)

Srinivasan Sivasubramanian Sundhar Mohandas Vidya Gopalan Karthikeyan Govindan Poovazhagi Varadarajan

10.1016/j.yexmp.2022.104821

Cite

Citations (2)

Hepato‐protective effects of R‐phycoerythrin‐rich protein extract ofPortieria hornemannii(Lyngbye) Silva against DEN‐induced hepatocellular carcinoma

Journal of Food Biochemistry (2018)

N Senthilkumar Ramar Thangam Pitchai Murugan Suresh Veeraperumal Chidambaram Kurinjimalar

The present investigation evaluates the hepato-protective potential of R-phycoerythrin (R-PE)-rich protein extract obtained from Portieria hornemannii (Lyngbye) Silva against H2O2-induced hepatotoxicity using HepG2 cells (in vitro) and male Wistar albino Hepatocellular carcinoma (HCC) rats (in vivo). H2O2 treatment led to dose-dependent decreases in cell viability of HepG2, which was ameliorated by the treatment with R-PE-rich extract. In vivo studies showed that all the vital parameters including enzymatic and nonenzymatic antioxidants as well as liver marker enzymes were affected in the experimentally induced HCC male Wistar rats upon exposure to the N-diethylnitrosamine (DEN), a carcinogen. However, there was moderate to better restoration of such parameters in the carcinoma rats treated with R-PE-rich protein extract. Practical applications Therapeutic alternatives originating from food or food supplements are gaining popularity as "nutritional therapy" and they are well studied for their chemo-preventive effects. Investigations of several food-derived bioactive compounds revealed their ability to antagonize dysregulated targets in cellular signaling pathways to exhibit antineoplastic activities. Isolation of bioactive molecules present in marine food products and determination of their broad range pharmaceutical activity by means of deducing specific molecular targets as well as establishing minimal toxicity to normal tissues could aid in treatment of cancer. Phycobiliproteins are an important group of pigment molecules extracted from the red and blue green algae. A study was envisaged to evaluate the anticancer potential of RPERPE (R-PE-rich protein extract) obtained from P. hornemannii (Lyngbye) Silva against HCC using male Wistar albino rats as models. This study offers the scope of using the R-PE-rich protein extract of P.hornemannii for developing therapeutic anticancer formulation.

Phycobiliprotein

Phycoerythrin

Viability assay

Functional food

10.1111/jfbc.12695

Cite

Citations (16)

Phenotypic Screening Identifies Synergistically Acting Natural Product Enhancing the Performance of Biomaterial Based Wound Healing

Frontiers in Pharmacology (2017)

Srinivasan Sivasubramanian Gayathri Chandrasekar Sara Svensson Akusjärvi Ramar Thangam Malairaj Sathuvan

The potential of multifunctional wound heal biomaterial relies on the optimal content of therapeutic constituents as well as the desirable physical, chemical and biological properties to accelerate the healing process. Formulating biomaterials such as Amnion or collagen based scaffolds with natural products offer an affordable strategy to develop dressing material with high efficiency in healing wounds. Using image based phenotyping and quantification, we screened natural product derived bioactive compounds for modulators of type I and III collagen production from human foreskin derived fibroblast cells. The identified hit was then formulated with amnion to develop a biomaterial, and its biophysical properties, in vitro and in vivo effects were characterized. In addition, we performed functional profiling analyses by PCR array to understand the effect of individual components of these materials on various genes such as inflammatory mediators including chemokines and cytokines, growth factors, fibroblast stimulating markers for collagen secretion, matrix metalloproteinases (MMPs) etc associated with wound healing. FACS based cell cycle analyses were carried out to evaluate the potential of biomaterials for induction of proliferation of fibroblasts. Western blot analyses was done to examine the effect of biomaterial on collagen synthesis by cells and compared to cells grown in the presence of growth factors. This work demonstrated an uncomplicated way of identifying components that synergistically promote healing. Besides, we demonstrated that modulating local wound environment using biomaterials with bioactive compounds could enhance healing. This study finds that the developed biomaterials offer immense scope for healing wounds by means of their skin regenerative features such as anti-inflammatory, fibroblast stimulation for collagen secretion as well as inhibition of enzymes and markers impeding the healing, hydrodynamic properties complemented with other features including non toxicity, biocompatibility and safety.

Natural product

Biomaterial

10.3389/fphar.2017.00433

Cite

Citations (3)

Role of lung ultrasound in diagnosing and differentiating transient tachypnea of the newborn and respiratory distress syndrome in preterm neonates

Journal of Ultrasonography (2022)

Srinivasan Sivasubramanian Neeti Aggarwal Sushma Makhaik Anupam Jhobta Sumala Kapila

Aim: To evaluate the accuracy of lung ultrasound in diagnosing and differentiating transient tachypnea of the newborn and respiratory distress syndrome in preterm neonates. Material and methods: This was a single-center study. From January 2020 to June 2021. A total of 100 preterm neonates, admitted to the neonatal intensive care unit with symptoms of respiratory distress within six hours of birth, including 50 diagnosed with transient tachypnea of the newborn and 50 with respiratory distress syndrome on the basis of clinical examination, laboratory testing, chest X-rays, were recruited in the study. Lung ultrasound was performed in each neonate by a senior radiologist who was blinded to the clinical diagnosis. Lung ultrasound findings in both conditions were analyzed and compared. Results: Pulmonary edema manifesting as alveolar-interstitial syndrome, double lung point sign and less commonly as white out lungs in the absence of consolidation has 100% sensitivity and specificity in diagnosing transient tachypnea of the newborn. A combination of three signs of consolidation with air or fluid bronchograms, white out lungs and absent spared areas has 100% sensitivity and specificity for diagnosing respiratory distress syndrome. Double lung point sign was seen only in infants suffering from transient tachypnea of the newborn and consolidation with air or fluid bronchograms only in cases of respiratory distress syndrome. Conclusion: Lung ultrasound can accurately diagnose and reliably differentiate transient tachypnea of the newborn and respiratory distress syndrome in preterm neonates. It has advantages that cannot be replicated by chest radiography. Lung ultrasound may be used as an initial screening tool.

Tachypnea

10.15557/jou.2022.0001

Cite

Citations (13)

HER2 Targeted Breast Cancer Therapy with Switchable “Off/On” Multifunctional “Smart” Magnetic Polymer Core–Shell Nanocomposites

ACS Applied Materials & Interfaces (2016)

Raju Vivek Ramar Thangam Selvaraj Rajesh Kumar Chandrababu Rejeeth Srinivasan Sivasubramanian

Multifunctional magnetic polymer nanocombinations are gaining importance in cancer nanotheranostics due to their safety and their potential in delivering targeted functions. Herein, we report a novel multifunctional core-shell magnetic polymer therapeutic nanocomposites (NCs) exhibiting pH dependent "Off-On" release of drug against breast cancer cells. The NCs are intact in blood circulation ("Off" state), i.e., at physiological pH, whereas activated ("On" state) at intracellular acidic pH environment of the targeted breast cancer cells. The NCs are prepared by coating the cannonball (iron nanocore) with hydrophobic nanopockets of pH-responsive poly(d,l-lactic-co-glycolic acid) (PLGA) polymer nanoshell that allows efficient loading of therapeutics. Further, the nanocore-polymer shell is stabilized by poly(vinylpyrrolidone) (PVP) and functionalized with a targeting HER2 ligand. The prepared Her-Fe3O4@PLGA-PVP nanocomposites facilitate packing of anticancer drug (Tamoxifen) without premature release in the bloodstream, recognizing the target cells through binding of Herceptin antibody to HER2, a cell surface receptor expressed by breast cancer cells to promote HER2 receptor mediated endocytosis and finally releasing the drug at the intracellular site of tumor cells ("On" state) to induce apoptosis. The therapeutic efficiency of hemo/cytocompatible NCs drug delivery system (DDS) in terms of targeted delivery and sustained release of therapeutic agent against breast cancer cells was substantiated by in vitro and in vivo studies. The multifunctional properties of Her-Tam-Fe3O4@PLGA-PVP NCs may open up new avenues in cancer therapy through overcoming the limitations of conventional cancer therapy.

PLGA

Targeted drug delivery

10.1021/acsami.5b11103

Cite

Citations (51)

Combinatorial nanocarrier based drug delivery approach for amalgamation of anti-tumor agents in breast cancer cells: an improved nanomedicine strategy

Scientific Reports (2016)

Chandran Murugan Kathirvel Rayappan Ramar Thangam Ramasamy Bhanumathi Krishnamurthy Shanthi

Abstract Combination therapy of multiple drugs through a single system is exhibiting high therapeutic effects. We investigate nanocarrier mediated inhibitory effects of topotecan (TPT) and quercetin (QT) on triple negative breast cancer (TNBC) (MDA-MB-231) and multi drug resistant (MDR) type breast cancer cells (MCF-7) with respect to cellular uptake efficiency and therapeutic mechanisms as in vitro and in vivo . The synthesized mesoporous silica nanoparticle (MSN) pores used for loading TPT; the outer of the nanoparticles was decorated with poly (acrylic acid) (PAA)-Chitosan (CS) as anionic inner-cationic outer layer respectively and conjugated with QT. Subsequently, grafting of arginine-glycine-aspartic acid (cRGD) peptide on the surface of nanocarrier (CPMSN) thwarted the uptake by normal cells, but facilitated their uptake in cancer cells through integrin receptor mediated endocytosis and the dissociation of nanocarriers due to the ability to degrade CS and PAA in acidic pH, which enhance the intracellular release of drugs. Subsequently, the released drugs induce remarkable molecular activation as well as structural changes in tumor cell endoplasmic reticulum, nucleus and mitochondria that can trigger cell death. The valuable CPMSNs may open up new avenues in developing targeted therapeutic strategies to treat cancer through serving as an effective drug delivery podium.

Nanocarriers

Triple-negative breast cancer

10.1038/srep34053

Cite

Citations (126)