Pandemic V. cholerae strains in the O1 serogroup have 2 biotypes: classical and El Tor. The classical biotype strains of the sixth pandemic, which encode the classical type cholera toxin (CT), have been replaced by El Tor biotype strains of the seventh pandemic. The prototype El Tor strains that produce biotype-specific cholera toxin are being replaced by atypical El Tor variants that harbor classical cholera toxin. Atypical El Tor strains are categorized into 2 groups, Wave 2 and Wave 3 strains, based on genomic variations and the CTX phage that they harbor. Whole-genome analysis of V. cholerae strains in the seventh cholera pandemic has demonstrated gradual changes in the genome of prototype and atypical El Tor strains, indicating that atypical strains arose from the prototype strains by replacing the CTX phages. We examined the molecular mechanisms that effected the emergence of El Tor strains with classical cholera toxin-carrying phage. We isolated an intermediary V. cholerae strain that carried two different CTX phages that encode El Tor and classical cholera toxin, respectively. We show here that the intermediary strain can be converted into various Wave 2 strains and can act as the source of the novel mosaic CTX phages. These results imply that the Wave 2 and Wave 3 strains may have been generated from such intermediary strains in nature. Prototype El Tor strains can become Wave 3 strains by excision of CTX-1 and re-equipping with the new CTX phages. Our data suggest that inter-chromosomal recombination between 2 types of CTX phages is possible when a host bacterial cell is infected by multiple CTX phages. Our study also provides molecular insights into population changes in V. cholerae in the absence of significant changes to the genome but by replacement of the CTX prophage that they harbor.
가우스 괄호법을 이용하여 무한 물점을 포함한 모든 유한 물점을 대상으로 하는 모든 복잡한 줌 렌즈에서 사용가능한 일반적인 근축광선 줌 궤적 추적식을 유도하였다. 이를 Visual Basic으로 프로그램화하여 수치해석적으로 줌 궤적을 구하였다. 이 결과 이 식의 해는 물체의 거리에 관계없이 모든 종류의 줌 렌즈에서 줌 궤적에 대한 초기설계에 유연하면서 통합적으로 적용할 수 있다. 이 식의 유용성을 증명하기 위하여 $M_{4a}$와 $M_{4h}$ 형태의 4군 줌 렌즈들과 $M_{5n}$ 형태의 5군 줌 렌즈의 줌 궤적을 유한 물점에 대해서 빠르게 산출할 수 있음을 보였다. We theoretically derive the set of general paraxial zoom locus equations for all zoom lens systems with finite object distance, including the infinite object distance case, by using the Gaussian bracket method and matrix representation of paraxial ray tracing. We make the zoom locus program by means of a numerical calculation method according to these equations in Visual Basic Language. Consequently, the solutions of this method can be consistently and flexibly used in all types of zoom lens in the step of initial design about zoom loci. Finally, in order to verify the justification and usefulness of this method, we show that two examples, such as $M_{4a}$ and $M_{4h}$ types of 4 groups, and one example, $M_{5n}$ type of 5 groups, which are very complicated zoom lens systems, can be rapidly and diversely traced through various interpolations by using this program.
Results from a post-marketing study to generate evidence on 1-year antibody persistence and safety following vaccination of infants from South Korea with the quadrivalent meningococcal conjugate vaccine MenACWY-CRM.In this phase IV, open-label, multi-center study (NCT02446691), 128 infants received MenACWY-CRM at ages 2, 4, 6, and 12 months. One-year antibody persistence following the full vaccination course was evaluated (primary objective) for the four meningococcal serogroups (Men) by serum bactericidal activity assay using human or rabbit complement (hSBA/rSBA). Immune responses at 1-month post-vaccination and safety were also assessed.The percentage of children with hSBA titers ≥8 ranged between 94% (MenA) and 100% (MenY/W) 1-month post-vaccination, and from 39% (MenA) to 89% (MenY) 1-year post-vaccination. At least 99% and 92% of children had rSBA titers ≥8 and ≥128 against each meningococcal serogroup, 1-month post-vaccination. One-year post-vaccination, the percentage of children with rSBA titers ≥8 and ≥128 ranged from 54% (MenC) to 99% (MenA) and from 30% (MenC) to 98% (MenA). Geometric mean titers declined from 1-month to 1-year post-vaccination, when they varied between 6.8 (MenA) and 53.6 (MenW) by hSBA and between 17.2 (MenC) and 2,269.5 (MenA) by rSBA. At least one solicited and unsolicited adverse event was reported for 79% and 66% of children. Of 36 serious adverse events reported, none were vaccination-related.Antibody persistence (hSBA/rSBA titers ≥8) was determined in 39%-99% of children 1 year after a 4-dose MenACWY-CRM series during infancy, with an acceptable clinical safety profile.
M Z O thin films with various composition x of ZnO were fabricated by a RF magnetron sputtering method, which is expected to improve the electro-optical properties of the conventional MgO protective layer for AC-PDP. Test panels with the Z O protective layer have been fabricated in order to investigate the effects of ZnO doping on the electrical characteristics of devices such as the discharge voltages and the memory gain. Experimental results revealed that test panels with the Z O(x=0.5at%) protective layer show lower firing and sustain voltages than those seen in panels with MgO protective layer by 20V. resulting in an increasement of the memory coefficient. In addition, it was found that test panels with the Z O protective layer show higher discharge intensity, i. e., higher plasma density, compared with panels with MgO protective layer.ve layer.layer.
Free and forced vibration analysis of the global ship structure using the 3-dimensional finite element(FE) method requires not only the specialized knowledge such as ship structure interacted with fluid, damping and various excitations due to propulsion system but also time-consuming manual tasks in FE modeling, analysis and response evaluation. As a result, the quality of the vibration analysis highly depends on engineer's expertise and experience. In this study, a framework system to improve the efficiency of global ship vibration analysis is introduced. The system promising the utilization of MSC/Patran and MSC/Nastran consists of various modules to support data management, FE modeling of ship structure and loading, input deck generation for free and forced vibration analysis, data extraction and evaluation of analysis results, and databases for FE models of marine diesel engines and vibration criteria. The system may be useful for pursuing standardization of uncertain analysis factors as well as reducing time, cost and human dependency in ship vibration analysis.
새로운 티타늄기 MMCs(W/ Ti-6Al-4V)에 대한 기계적 특성에 대한 연구를 행하여 평가한 결과를 나타내었다. HIP(hot isostatic pressing) 제조법과 RS(Rotary Swaging) 2 차 가공을 통하여 텅스텐 섬유 함유율이 각각 6, 9, 12 vol%인 W/Ti-6Al-4V MMCs 를 제작하였으며, 경도는 기존의 Ti-6Al-4V 합금과 비교하여 20-30%, 인장강도는 50%(비강도-38%) 높은 값을 얻었다. 섬유 함유율 9vol.% 에서 가장 높은 인장 값을 나타내었으며, MMCs 에서 메트릭스와 계면 사이에 생성된 확산상에 의해서 경도 및 인장강도가 향상되었다. 또한 피로 강도를 향상 시키기 위한 방법으로 메트릭스의 질적 향상을 위한 HIP 제작조건에 대한 추가적인 연구가 필요하다.
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