Background Co-circulation of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and other respiratory viruses, such as influenza and respiratory syncytial virus (RSV), can be a severe threat to public health. The accurate detection and differentiation of these viruses are essential for clinical laboratories. Herein, we comparatively evaluated the performance of the Kaira COVID-19/Flu/RSV Detection Kit (Kaira; Optolane, Seongnam, Korea) for detection of SARS-CoV-2, influenza A and B, and RSV in nasopharyngeal swab (NPS) specimens with that of the PowerChek SARS-CoV-2, Influenza A&B, RSV Multiplex Real-time PCR Kit (PowerChek; Kogene Biotech, Seoul, Korea). Methods A total of 250 archived NPS specimens collected for routine clinical testing were tested in parallel by the Kaira and PowerChek assays. RNA standards were serially diluted and tested by the Kaira assay to calculate the limit of detection (LOD). Results The positive and negative percent agreements between the Kaira and PowerChek assays were as follows: 100% (49/49) and 100% (201/201) for SARS-CoV-2; 100% (50/50) and 99.0% (198/200) for influenza A; 100% (50/50) and 100% (200/200) for influenza B; and 100% (51/51) and 100% (199/199) for RSV, respectively. The LODs of the Kaira assay for SARS-CoV-2, influenza A and B, and RSV were 106.1, 717.1, 287.3, and 442.9 copies/mL, respectively. Conclusions The Kaira assay showed comparable performance to the PowerChek assay for detection of SARS-CoV-2, influenza A and B, and RSV in NPS specimens, indicating that the Kaira assay could be a useful diagnostic tool when these viruses are co-circulating.
Yoo Na Chung, M.D., In Young Yoo, M.D., Ph.D., Sun Ae Yun, M.T., Ji-Youn Kim, M.T., Nam Yong Lee, M.D., Ph.D., and Hee Jae Huh, M.D., Ph.D.. Ann Lab Med 2021;41:506-9. https://doi.org/10.3343/alm.2021.41.5.506
Abstract It has been suggested that periodontitis is associated with metabolic abnormalities including non-alcoholic fatty liver disease (NAFLD). The fatty liver index (FLI) is a non-invasive surrogate marker and predictor of NAFLD. We aimed to determine whether FLI itself would be associated with periodontitis through a secondary analysis of previously reported nationally representative probability sample data of the Korean population. FLI was calculated from a previously developed algorithm which combines measures of body mass index (BMI), waist circumference, triglyceride, and gamma-glutamyl transferase (GGT). Periodontitis was diagnosed based on the Community Periodontal Index (CPI) developed by the World Health Organization. Of 4,272 participants, 26.1% were diagnosed with periodontitis. Higher FLI was associated with a higher prevalence of periodontitis (Odds ratio (OR) highest vs. lowest quartile of FLI ,1.63; 95% confidence interval (CI), 1.23–2.16; P = 0.001 for trend) adjusting for confounding factors. In the highest FLI quartile, prevalence of periodontitis was higher in individuals with diabetes (OR highest vs. lowest quartile of FLI , 2.89; 95% CI, 1.01–8.27 for diabetic subgroup; OR highest vs. lowest quartile of FLI , 1.45; 95% CI, 1.07–1.96 for non-diabetic subgroup). In summary, FLI was associated with prevalent periodontitis.
In dentistry, tissue expanders have been used to obtain sufficient soft tissue for alveolar bone augmentation in the severely atrophic ridge. Herein, we review two cases of soft tissue augmentation using a self-inflating tissue expander in patients in the Department of Oral and Maxillofacial Surgery at Ewha Womans University Mokdong Hospital for bone graft and implant operations. The results of each patient were presented using pre-operative and post-operative radiographs and clinical exams. The results of our study indicate successful bone graft and implant surgery using a self-inflating tissue expander.
본 연구는 대표적인 식량 작물인 밀과 보리의 새싹을 쌍기어를 장착한 가정용 저속 녹즙기로 착즙하여 제조한 각각의 새싹 주스에 대해 주스의 품질 특성을 조사하고 주스 섭취 후 소화 과정에 따른 폴리페놀 함량 변화와 그에 따른 항산화 활성 및 ACE 저해능 분석을 통해 밀싹과 새싹보리의 대사활성 변화를 조사하기 위해 수행되었다. 밀싹과 새싹보리의 착즙을 통해 분쇄되는 입자의 평균 크기는 117.7 μm 및 203.1 μm로 미세한 입자들이 주스에 분포되어 있음을 확인 하였다. 그뿐만 아니라 밀싹과 새싹보리 주스에는 chlorophyll과 protease 효소를 포함한 다양한 영양성분이 풍부하게 함유되어 있으며, 특히 비타민 C와 폴리페놀 및 플라보노이드를 풍부하게 함유하고 있어 SOD 유사 활성 및 지질과산화 억제능 시험에서 높은 항산화 활성을 보였다. 이들 주스의 섭취에 따른 대사활성 변화를 조사하기 위해 위장 및 소장 소화를 in vitro digestion model을 통해 재현하였다. 가장 먼저 소화 과정에 따른 폴리페놀 및 플라보노이드 함량을 분석한 결과 위장 소화 단계를 거치면서 함량이 크게 증가하는 것을 확인하였으며, 이에 따라 밀싹과 새싹보리 주스의 환원력과 ABTS 라디칼 소거능 또한 크게 증가하는 것을 확인하였다. 폴리페놀 및 플라보노이드 함량 증가와 FRAP 및 ABTS 소거능 간의 상관계수는 모두 0.78 이상으로 높은 양의 상관관계를 보였다. 또한 소화 과정에 따른 ACE 저해 활성을 조사한 결과 위장 소화 단계에서 저해능이 매우 증가하는 것을 확인하였으나 소장 소화를 거치면 오히려 감소하는 것을 확인하였다. 따라서 본 연구에서는 밀싹과 새싹보리의 주스 품질 특성을 확인하였으며 주스 섭취에 따라 위장 및 소장 소화와 같은 대사 변화를 통해 생리활성이 크게 증가할 수 있음을 확인하였다.
We evaluated the performance of two different array-based techniques, a bead-based multiplex genotyping method (LQ; digene HPV Genotyping LQ Test, QIAGEN, Germany) and a DNA chip-based method using peptide nucleic acid probes (PANArray; PANArray HPV Genotyping Chip, Panagene, Korea), for detection of human papillomavirus (HPV) and genotyping of high-risk (HR) or probable high-risk (PHR) HPVs in healthy patients who visited a health-promotion center.We obtained 508 unselected, consecutive cervicovaginal swab specimens. All specimens were examined by using the PANArray and LQ tests. All HPV-positive samples were then analyzed by multiplex PCR and direct sequencing.The LQ test detected 47 HPV-positive cases (9.3%) with HR or PHR genotypes and the PANArray test identified 36 cases (7.1%). When the results of LQ and PANArray were compared by using comprehensive genotyping (integrated interpretation of the results of LQ, PANArray, multiplex PCR, and direct sequencing) for the detection of HR or PHR genotypes, the kappa values were 0.44 and 0.30 for LQ and PANArray, respectively. In comparison to comprehensive genotyping, the LQ test yielded 53 (60.0%) concordant and 12 (13.5%) compatible results, and the PANArray yielded 36 (40.4%) concordant and three (3.4%) compatible results.The results of the LQ test had higher concordance and/or greater compatibility with those of comprehensive genotyping for the detection of HR or PHR genotypes than those of the PANArray test.
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