Taking land consolidation project in Binchuan County of Yunnan Province as a case,according to relevant statistical data and results of questionnaire survey,before-after comparison method as well as dynamic and static economic benefit index was adopted to study the economic benefit of land consolidation in project area,and analyze the causes of the difference between real economic benefit and predicted economic benefit of land consolidation.
Related studies have provided significant insights into polyploid breeding in recent years, but limited research was focused on trees. The genomic information for the growth and response to abiotic stress in polyploidy trees is still largely unknown. Populus alba ’ Berolinensis ’, also named “Yinzhong poplar”, is a triploid poplar in the northeast of China. This hybrid triploid poplar is widely used as landscape ornamentals in urban areas because of its fast growth and high tolerance to abiotic stress. As an artificially synthesized male allotriploid hybrid, the three monoploid genomes of P. alba ’ Berolinensis ’ originated from different poplar species, so it is the desired material for studying polyploidy genomic collaboration mechanisms. Therefore, we intensively studied the allelic genomic collaboration mechanism in P. alba ’ Berolinensis ’. This study generated a high-quality chromosome-level genome assembly for the P. alba ’ Berolinensis ’ consisting of 19 allelic chromosomes. Its three monoploid chromosomes are polymorphic with an average of 42.22 variant sites per allelic gene locus. Meanwhile, we found that stress related genes such as RD22 and LEA7 exhibited structure variations. The above information has all been deployed to our polyploid genome online analysis website TreeGenomes (https://www.treegenomes.com). These polyploid genomic related resources will provide critical foundations for the molecular breeding of P. alba ’ Berolinensis ’ and help us uncover the allopolyploidization effects on the resistance and traits of polyploidy species deeper in the future.
Dongfudou 3 is a highly sought-after soybean variety due to its lack of beany flavor. To support molecular breeding efforts, we conducted a genomic survey using next-generation sequencing. We determined the genome size, complexity, and characteristics of Dongfudou 3. Furthermore, we constructed a chromosome-level draft genome and speculated on the molecular basis of protein deficiency in GmLOX1, GmLOX2, and GmLOX3. These findings set the stage for high-quality genome analysis using third-generation sequencing. The estimated genome size is approximately 1.07 Gb, with repetitive sequences accounting for 72.50%. The genome is homozygous and devoid of microbial contamination. The draft genome consists of 916.00 Mb anchored onto 20 chromosomes, with annotations of 46,446 genes and 77,391 transcripts, achieving Benchmarking Single-Copy Orthologue (BUSCO) completeness of 99.5% for genome completeness and 99.1% for annotation. Deletions and substitutions were identified in the three GmLox genes, and they also lack corresponding active proteins. Our proposed approach, involving k-mer analysis after filtering out organellar DNA sequences, is applicable to genome surveys of all plant species, allowing for accurate assessments of size and complexity. Moreover, the process of constructing chromosome-level draft genomes using closely related reference genomes offers cost-effective access to valuable information, maximizing data utilization.
Betula platyphylla is a common tree species in northern China that has high economic and medicinal value. Our laboratory has been devoted to genome research on B. platyphylla for approximately 10 years. As primary organelle genomes, the complete genome sequences of chloroplasts are important to study the divergence of species, RNA editing and phylogeny. In this study, we sequenced and analyzed the complete chloroplast (cp) genome sequence of B. platyphylla. The complete cp genome of B. platyphylla was 160,518 bp in length, which included a pair of inverted repeats (IRs) of 26,056 bp that separated a large single copy (LSC) region of 89,397 bp and a small single copy (SSC) region of 19,009 bp. The annotation contained a total of 129 genes, including 84 protein-coding genes, 37 tRNA genes and 8 rRNA genes. There were 3 genes using alternative initiation codons. Comparative genomics showed that the sequence of the Fagales species cp genome was relatively conserved, but there were still some high variation regions that could be used as molecular markers. The IR expansion event of B. platyphylla resulted in larger cp genomes and rps19 pseudogene formation. The simple sequence repeat (SSR) analysis showed that there were 105 SSRs in the cp genome of B. platyphylla. RNA editing sites recognition indicated that at least 80 RNA editing events occurred in the cp genome. Most of the substitutions were C to U, while a small proportion of them were not. In particular, three editing loci on the rRNA were converted to more than two other bases that had never been reported. For synonymous conversion, most of them increased the relative synonymous codon usage (RSCU) value of the codons. The phylogenetic analysis suggested that B. platyphylla had a closer evolutionary relationship with B. pendula than B. nana. In this study, we not only obtained and annotated the complete cp genome sequence of B. platyphylla, but we also identified new RNA editing sites and predicted the phylogenetic relationships among Fagales species. These findings will facilitate genomic, genetic engineering and phylogenetic studies of this important species.
The deposition and distribution pattern of pesticide droplets on crop leaves has a close link with the efficiency of pesticide application.The research on the deposition and distribution pattern of pesticide droplets has contributed to decreasing the pesticide pollution and the pesticide residual in crops.The significance for the research on the deposition and distribution pattern of pesticide droplets,the current situation of the research both domestic and foreign,and the general research methods are introduced in this paper,which clarify the orientation of the research in this area.
Abstract Populus spp. have long been used as model woody plant species for molecular biology research. However, tissues of poplar are often recalcitrant to experimental procedures for molecular studies. We generated a hormone autotrophic poplar suspension cell line from a hybrid of Populus alba × P. berolinensis ‘Yinzhong’, named Qu-2. Qu-2 cells are suitable as a model biological system for studying woody plants. Qu-2 cells have many advantages over suspension cell lines derived so far from any other woody plants. Qu-2 cells are very easy to cultivate and can grow on several common plant culture media without the addition of any plant hormone. They show exceptionally high growth rates, reaching an approximately 150-fold increase in biomass after one week of culturing. Another important unique characteristic of Qu-2 cells is that they can be cryopreserved and readily reactivated. Qu-2 cells are suitable for molecular manipulations such as protoplast production, transient transformation, and RNA-seq analysis. Therefore, Qu-2 cells have the great potential to be an excellent model cell line in tree molecular biological research, ranging from physiology to gene function. The Qu-2 cells will be made available to the plant community for research.
The selenium rich and selenium tolerant ability of active dry yeast(ADY) and its conditions of bioconversion of inorganic selenium to the organic seleniun were studied. The results showed that ADY has strong selenium rich and selenium tolerant ability and the culture conditions have a great influence on selenium rich ability.The concentraction of inorganic selenium in medium and culture time,etc., were main factors affecting bioconversion of inorganic selenium. Under the initial optimum conditions(the mash liquor of 12 °Be, the inorganic selenium concentraction of 15 mg/L,medium of 60 mL in a 250 mL flask, initial pH=6.0, and culture time 30 h at 30 ℃), the dry weight of selenium yeast and the content of organic selenium in the cells as well as the rate of se bioconversion were 15.8 g/L,790.0 μg/g and 83.2% in proper order.
The social and economical condition,cultivated land resources and general situation of grain production of research area are introduced.According to relevant data of cultivated land of Kunming City in 2006,taking the minimum per capita cultivated land and index model of cultivated land pressure,and combining with two conditions of per capita grain demand which are 300 kg and 400 kg,the comparative research method was used to calculate and analyze the minimum per capita cultivated land and index model of cultivated land pressure.The results show that when per capita grain demand-Gr is 300 kg,the per capita cultivated land in Kunming City has regional differences,reflects different regular patterns of spatial distribution,and presents obvious and typical radial distribution.Urban area of Kunming has the lowest point and be taken as the center,per capita cultivated land decreases progressively from suburban to the periphery;when per capita grain demand-Gr is 400 kg,under the same consumption level,cultivated land pressures of each county in Kunming City are different,and so are the driven factors;when per capita grain demand-Gr is 300 kg or 400 kg,the indexes of cultivated land pressure in Kunming are all greater than 1,cultivated land pressure is enormous,the indexes of cultivated land pressure of each county(district) have two conditions-greater than 1 and less than 2,and the reasons differ from one another.Combining with the spatial distribution characteristics of the cultivated land and food and the situation of industrial development,the countermeasures and suggestions are put forwarded to make full use of back-up cultivated land,to plan the distribution of industrial structure in each county(district) of Kunming City as a whole,to dispose cultivated land resources reasonably and so on.
Abstract Background Soybean ( Glycine max ), a vital grain and oilseed crop, serves as a primary source of plant protein and oil. Soil salinization poses a significant threat to soybean planting, highlighting the urgency to improve soybean resilience and adaptability to saline stress. Melatonin, recently identified as a key plant growth regulator, plays crucial roles in plant growth, development, and responses to environmental stress. However, the potential of melatonin to mitigate alkali stress in soybeans and the underlying mechanisms remain unclear. Results This study investigated the effects of exogenous melatonin on the soybean cultivar Zhonghuang 13 under alkaline stress. We employed physiological, biochemical, transcriptomic, and metabolomic analyses throughout both vegetative and pod-filling growth stages. Our findings demonstrate that melatonin significantly counteracts the detrimental effects of alkaline stress on soybean plants, promoting plant growth, photosynthesis, and antioxidant capacity. Transcriptomic analysis during both growth stages under alkaline stress, with and without melatonin treatment, identified 2,834 and 549 differentially expressed genes, respectively. These genes may play a vital role in regulating plant adaptation to abiotic stress. Notably, analysis of phytohormone biosynthesis pathways revealed altered expression of key genes, particularly in the ARF (auxin response factor), AUX/IAA (auxin/indole-3-acetic acid), and GH3 (Gretchen Hagen 3) families, during the early stress response. Metabolomic analysis during the pod-filling stage identified highly expressed metabolites responding to melatonin application, such as uteolin-7-O-(2''-O-rhamnosyl)rutinoside and Hederagenin-3-O-glucuronide-28-O-glucosyl(1,2)glucoside, which helped alleviate the damage caused by alkali stress. Furthermore, we identified 183 differentially expressed transcription factors, potentially playing a critical role in regulating plant adaptation to abiotic stress. Among these, the gene SoyZH13_04G073701 is particularly noteworthy as it regulates the key differentially expressed metabolite, the terpene metabolite Hederagenin-3-O-glucuronide-28-O-glucosyl(1,2)glucoside. WGCNA analysis identified this gene ( SoyZH13_04G073701 ) as a hub gene, positively regulating the crucial differentially expressed metabolite of terpenoids, Hederagenin-3-O-glucuronide-28-O-glucosyl(1,2)glucoside. Our findings provide novel insights into how exogenous melatonin alleviates alkali stress in soybeans at different reproductive stages. Conclusions Integrating transcriptomic and metabolomic approaches, our study elucidates the mechanisms by which exogenous melatonin ameliorates the inhibitory effects of alkaline stress on soybean growth and development. This occurs through modulation of biosynthesis pathways for key compounds, including terpenes, flavonoids, and phenolics. Our findings provide initial mechanistic insights into how melatonin mitigates alkaline stress in soybeans, offering a foundation for molecular breeding strategies to enhance salt-alkali tolerance in this crop.
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