The elucidation of the relationship between the structure and function of biological macromolecules is greatly aided by the availability of appropriate mutants. The classical genetic approach depends on mutations that arise at random either spontaneously or as a consequence of mutagenic agents. Mutants are usually selected because of some desired phenotype or striking property. Difficulties may arise when the role of noncoding, functionally undefined segments of a genome are to be studied, since one does not know what property to screen or select for. Moreover, in haploid organisms, unconditionally lethal mutations cannot be picked up at all. The problems are compounded in the case of higher organisms, where recessive mutations are frequently only recognizable when homozygous. In addition, the genome of higher eukaryotes is so large that the probability of finding a mutation in a particular DNA region without a potent selection system is quite low.
We present energy spectra of electrons formed in the reaction of He(23 S, 21 S) with NO2 which have significantly improved counting statistics and resolution compared to earlier work. Further, we show spectra of the fluorescence light emitted in these reactions. The data are recorded in the same molecular beam apparatus as the electron spectra. For the metastable singlet state He(21 S) the spectra have not been measured before. We find that in addition to ionization excitation transfer takes place into Rydberg states of NO2**. Subsequently, the highly excited NO2** molecules dissociate into NO and atomic O* Rydberg atoms.
Patienten mit hämolytischen Erkrankungen und bei akuten und chronischen Nierenerkrankungen berichtet.Dabei wird gezeigt, daß die quantitative Haptoglobinbestimmung für die Beurteilung der entzündlichen Aktivität von rheumatischen Erkrankungen, Nierenerkrankungen und Lungenerkrankungen von Bedeutung ist.Auch für Diagnose und Verlaufsbeobachtung von Krankheitsbildern mit gesteigerter Hämolyse und zum Teil in Zusammenhang damit bei der Differentialdiaghose des Ikterus envies sich die quantitative Haptoglobinbestimmung von Wert.A simple, quantitative method for haptoglobin determination, which depends on JAYLES so-called activation method, is described.The method is suitable for both routine and small laboratory analyses.The results of quantitative haptoglobin determinations are given for 63 healthy individuals, 255 with rheumatic diseases, 34 with liver diseases, 83 with M. Boeck, 9 with haemolytic diseases and for acute and chronic kidney diseases.It is thus shown.that quantitative haptoglobin determination is significant for the diagnosis of inflammatory rheumatic, kidney and lung diseases.It is also useful for diagnosis and following the course of diseases involving increased haemolysis, and is thus an aid in the differential diagnosis of jaundice.Die a 2 -Serumhaptoglobine machen beim Gesunden etwa Zur Bestimmung des a 2 -Haptoglobins stehen folgende 20% der a 2 ;Globuline aus.Ausgedehnte
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