Objective To explore minimally invasive decompression mesenchymal stem cells combined with bone graft in the treatment of avascular necrosis of the femoral head in clinical application.Methods Select Jan 2009 to Jan 2011,from adopting minimally invasive decompression mesenchymal stem cells combined with bone graft in the treatment of avascular necrosis of the femoral head in 48 cases of clinical observations.Results 48 cases were followed up for 12~36 months,mean follow-up of 14 months.48 cases were confirmed by imaging examination,30 cases excellent,9 cases of benign,in 6 cases,poor in 3 cases,good in 39 cases,the excellent and good rate was 81.25%.Conclusion Minimally invasive decompression mesenchymal stem cells combined with bone graft in the treatment of avascular necrosis of the femoral head to core decompression after the effective support,promote new bone formation,reduction of the femoral head collapse,reduce the rate of disability.
Abstract Background Triple‐negative breast cancer (TNBC) is a highly heterogeneous and clinically aggressive disease. Accumulating evidence indicates that tertiary lymphoid structures (TLSs) and tumor budding (TB) are significantly correlated with the outcomes of patients who have TNBC, but no integrated TLS‐TB profile has been established to predict their survival. The objective of this study was to investigate the relationship between the TLS/TB ratio and clinical outcomes of patients with TNBC using artificial intelligence (AI)‐based analysis. Methods The infiltration levels of TLSs and TB were evaluated using hematoxylin and eosin staining, immunohistochemistry staining, and AI‐based analysis. Various cellular subtypes within TLS were determined by multiplex immunofluorescence. Subsequently, the authors established a nomogram model, conducted calibration curve analyses, and performed decision curve analyses using R software. Results In both the training and validation cohorts, the antitumor/protumor model established by the authors demonstrated a positive correlation between the TLS/TB index and the overall survival (OS) and relapse‐free survival (RFS) of patients with TNBC. Notably, patients who had a high percentage of CD8‐positive T cells, CD45RO‐positive T cells, or CD20‐positive B cells within the TLSs experienced improved OS and RFS. Furthermore, the authors developed a comprehensive TLS‐TB profile nomogram based on the TLS/TB index. This novel model outperformed the classical tumor‐lymph node‐metastasis staging system in predicting the OS and RFS of patients with TNBC. Conclusions A novel strategy for predicting the prognosis of patients with TNBC was established through integrated AI‐based analysis and a machine‐learning workflow. The TLS/TB index was identified as an independent prognostic factor for TNBC. This nomogram‐based TLS‐TB profile would help improve the accuracy of predicting the prognosis of patients who have TNBC.
Objective
To introduce Abdominal Surgery Impact Scale-Revised (ASIS-R) and to verify its reliability and validity in acute pancreatitis patients.
Methods
ASIS-R were translated into Chinese by 2 nursing postgraduates who passed College English Test-6 (CET-6) in accordance with Brislin's translation principle. The Chinese version of ASIS-R were then translated back into English by two nursing specialists who graduated abroad and were proficient both in English and Chinese. The scale was culturally adjusted through pre-survey. Totally 182 acute pancreatitis patients attending the Pancreas Center of a Class Ⅲ Grade A hospital in Jiangsu Province from October 2017 to April 2018 were selected by convenient sampling, and a total of 173 valid questionnaires were included to analyze the scale's reliability and validity in acute pancreatitis patients.
Results
The Chinese version of ASIS-R had 14 items in total, including limitation of motion, function impairment, visceral function, sleep and psychological function. Its Cronbach's α coefficient was 0.719, and that of different dimensions was 0.966, 0.964, 0.972, 0.976 and 0.968, respectively. The retest reliability was 0.660. Evaluated by the committee of experts, the content validity of various items in the Chinese version of ASIS-R (S-CVI/Ave) averaged 0.95, and their content validity index (I-CVI) ranged 0.80-1.00. Totally 5 common factors were extracted by exploratory factor analysis, which could account for 94.93% of the total variance. The factor loading of various items on their common factors were>0.4.
Conclusions
The Chinese version of ASIS-R has sound reliability and validity, which may serve as a tool for acute pancreatitis patients to evaluate the short-term quality of life in hospital by themselves.
Key words:
Quality of life; Acute pancreatitis; Reliability; Validity; Abdominal Surgery Impact Scale-Revised; Scale localization
Objective To evaluate the cytotoxicity and cell affinity of a porcine collagenic dermal matrix(PCDM) to guide the improvement of the porcine dermal scaffold.Methods The toxic effects of different concentrations of PCDM extract were tested by using MTT to assess the proliferation of mouse fibroblast(FB) cell line(L-929);the cytotoxicity of the target biomaterial PCDM was graded according to the national standards.The adhesion rate of the FB growing on the papillary side of PCDM was measured by MTT;the growth manner of the FB seeded on the papillary dermis for 1,7 and 14 days were studied after HE staining and under electronic microscope(SEM).Results The toxicity of PCDM was graded 0-1,which means the biomaterial has no cytotoxicity.MTT showed the number of cells adhered to the PCDM was only 50% that of the blank control culture plate.Microscopies showed the proliferation of FB on PCDM was quite well,but there were no cells infiltrating into the dermal matrix.Conclusion PCDM is a natural nontoxic xenogenic dermal substitute with good cell affinity,but further endeavors are needed to improve its abilities of adherence and cell compatibility to FB of the recipient.
Objective: Pancreatic ductal adenocarcinoma (PDAC) is an aggressive malignancy. CD8+ T cells, cancer stem cells (CSCs), and tumor budding (TB) have been significantly correlated with the outcome of patients with PDAC, but the correlations have been independently reported. In addition, no integrated immune-CSC-TB profile for predicting survival in patients with PDAC has been established. Methods: Multiplexed immunofluorescence and artificial intelligence (AI)-based comprehensive analyses were used for quantification and spatial distribution analysis of CD8+ T cells, CD133+ CSCs, and TB. In vivo humanized patient-derived xenograft (PDX) models were established. Nomogram analysis, calibration curve, time-dependent receiver operating characteristic curve, and decision curve analyses were performed using R software. Results: The established ‘anti-/pro-tumor’ models showed that the CD8+ T cell/TB, CD8+ T cell/CD133+ CSC, TB-adjacent CD8+ T cell, and CD133+ CSC-adjacent CD8+ T cell indices were positively associated with survival of patients with PDAC. These findings were validated using PDX-transplanted humanized mouse models. An integrated nomogram-based immune-CSC-TB profile that included the CD8+ T cell/TB and CD8+ T cell/CD133+ CSC indices was established and shown to be superior to the tumor-node-metastasis stage model in predicting survival of patients with PDAC. Conclusions: ‘Anti-/pro-tumor’ models and the spatial relationship among CD8+ T cells, CSCs, and TB within the tumor microenvironment were investigated. Novel strategies to predict the prognosis of patients with PDAC were established using AI-based comprehensive analysis and machine learning workflow. The nomogram-based immune-CSC-TB profile can provide accurate prognosis prediction for patients with PDAC.
Abstract Acute pancreatitis (AP) is a common digestive disease characterized by inflammation of the pancreas. MiR-155 plays a role in promoting inflammation and inhibiting the activation of anti-inflammatory pathways. Impaired autophagy could promote zymogen activation, abnormal acinar cell secretion, cell death, and the inflammatory response to aggravate AP. The aim of this study was to ascertain the effect of silencing miR-155 on AP through its effects on inflammation and impaired autophagy in vivo. In this study, AAV(adeno-associated virus)-mediated miR-155 and miR-155 sponge were injected through the tail vein of mice. After 3 weeks, AP was induced by intraperitoneal (IP) injections of cerulein. Pancreatic and pulmonary tissues were analyzed after 24 h. Silencing of miR-155 ameliorated pancreas and lung damage in three AP models of mice by preventing accumulation of autophagosomes that are unable to fuse with lysosomes and decreasing pancreatic inflammation by targeting TAB2. 3-MA could reduce the aberrant accumulation of autophagosomes, which alleviates the pancreas damage that was aggravated by increasing miR-155 levels. These findings demonstrate that the inhibition of miR-155 holds promise for limiting pancreatitis.
Neuroligin1 is an important synaptic cell adhesion molecule that modulates the function of synapses through protein–protein interactions. Yet, it remains unclear whether the regulation of synaptic transmission in the spinal cord by neruoligin1 contributes to the development of postoperative pain. In a rat model of postoperative pain induced by plantar incision, we conducted Western blot study to examine changes in the expression of postsynaptic membrane of neuroligin1, postsynaptic density 95 (PSD-95), and α-amino-3-hydroxy-5-methyl-4-isoxazole propionate (AMPA) receptor GluA1 and GluA2 subunits in the spinal cord dorsal horn after injury. The interaction between neuroligin1 and PSD-95 was further determined by using coimmunoprecipitation. Protein levels of neuroligin1 and GluA1, but not GluA2 and PSD-95, were significantly increased in the postsynaptic membrane of the ipsilateral dorsal horn at 3 h and 1 day after incision, as compared to that in control group (naïve). A greater amount of PSD-95 was coimmunoprecipitated with neuroligin1 at 3 h after incision than that in the control group. Intrathecal administration of small interfering RNAs (siRNAs) targeting neuroligin1 suppressed the expression of neuroligin1 in the spinal cord. Importantly, pretreatment with intrathecal neuroligin1 siRNA 2497 , but not scrambled siRNA or vehicle, prevented the upregulation of GluA1 expression at 3 h after incision, inhibited the enhanced neuroligin1/PSD-95 interaction, and attenuated postoperative pain. Together, current findings suggest that downregulation of spinal neuroligin1 expression may ameliorate postoperative pain through inhibiting neuroligin1/PSD-95 interaction and synaptic targeting of GluA1 subunit. Accordingly, spinal neuroligin1 may be a potential new target for postoperative pain treatment.
The H9N2 avian influenza virus (AIV) remains a serious threat to the global poultry industry and public health. The hemagglutinin (HA) protein is an essential protective antigen of AIVs and a major target of neutralizing antibodies and vaccines. Therefore, in this study, we used rice-derived HA protein as an immunogen to generate monoclonal antibodies (mAbs) and screened them using an immunoperoxidase monolayer assay and indirect enzyme-linked immunosorbent assay. Eight mAbs reacted well with the recombinant H9N2 AIV and HA protein, four of which exhibited potent inhibitory activity against hemagglutination, while three showed remarkable neutralization capacities. Western blotting confirmed that two mAbs bound to the HA protein. Linear epitopes were identified using the mAbs; a novel linear epitope, 480HKCDDQCM487, was identified. Structural analysis revealed that the novel linear epitope is located at the C-terminus of HA2 near the disulfide bond-linked HA1 and HA2. Alignment of the amino acid sequences showed that the epitope was highly conserved among multiple H9N2 AIV strains. The results of this study provide novel insights for refining vaccine and diagnostic strategies and expand our understanding of the immune response against AIV.
Objective To evaluate the use of rhomboid skin flap in expanded skin flap transfer. Methods If the top area of the rotation skin flap, when fully expanded, could not be maximally utilized, a rhomboid skin flap was redesigned to accommodate it. Results In the 9 cases treated with redesigned rhomboid skin flap in the rotation skin flap, all of the redesigned flaps survived, with some blood clot at the tip in one or two cases. Conclution The present design will enable the expanded soft tissue to be fully and rationally utilized to repair the skin defect. This method is safe and worthy of recommendation.
Objective To explore the effects of recombinant plasmids of pGPU6/GFP/NeoshRNA-CTGF (shRNA-CTGF) on the type Ⅰ collagen (COL-Ⅰ) protein expression in keloid,through RNA interference on connective tissue growth factor (CTGF) in vivo and in vitro.Methods Recombinant plasmids were designed and constructed by specific shRNA-CTGF; After transfeced human keloid fibroblast with shRNA-CTGF in vitro,RT-PCR was used to detect the CTGF mRNA level,and Western blot to detect the secretion of COL-Ⅰ.After transfected the keloid of nude mice with shRNA-CTGF in vivo,RT-PCR was used to detect the CTGF and COL-Ⅰ mRNA level,and Western blot was used to detect the protein expression of COL-Ⅰ.Results Recombinant plasmids of CTGF were successfully constructed,and the CTGF gene expression was significantly decreased in vivo and in vitro by 86.8％ and 54.1 ％,respectively; Down-regulation of CTGF in vitro significantly inhibited the mRNA and protein level of COL-Ⅰ by 76.8％ and 65.6％,respectively; Down-regulation of CTGF in vivo significantly reduced the COL-Ⅰ mRNA and protein level by 52.7％ and 48.0％,respectively.Conclusions CTGF gene expression is successfully down-regulated by the recombinant plasmid of shRNA-CTGF in vivo and in vitro.shRNA-CTGF significantly reduces the COL-Ⅰ protein level in keloid.It implies that CTGF gene is a potential target in the therapy of pathological scar.
Key words:
Keloid; Connective tissue growth factor (CTGF) ; Fibroblast; RNA interference (RNAi)
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