Wen Wu

Tianjin Medical University

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Wanqi Zhang

Shantou University

Wenxing Guo

Tianjin Medical University

Ziyun Pan

Tianjin Medical University

Zhiyuan Ren

Southern University of Science and Technology

Elizabeth N. Pearce

Boston University

Ying Yang

Tianjin Medical University

Shaohan Li

Southeast University

Naifan Zhang

Xinjiang Medical University

Kexin Zhang

Ocean University of China

Min Gao

Tianjin People's Hospital

Cooperative Institutions

Tianjin Medical University

Capital Medical University

Sun Yat-sen University

Fujian Medical University

Chinese Academy of Medical Sciences & Peking Union Medical College

Shanghai Jiao Tong University

Tianjin Medical University General Hospital

Sichuan University

Second Affiliated Hospital of Fujian Medical University

Chinese Academy of Sciences

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Clinical Observation on the effect of the Sigmoido-rectal bladder operation as a content urine reservoir after cystectomy

Wen Wu

OBJECTIVE: To introduce the Sigmoido-rectal bladder operation as a content urine reservoir after radical cystectomy. METHODS: 5 patients of bladder tumor who have the indication of radial cystectomy underwent sigmoido-rectal.RESULTS: No serious complications occurred during the period of postoperative. Defecation and urination could be automatically controlled by external spkincter muscle of anus. After 1.5～2 months, feces is every two hours or so, and the life become normal.

Urination

Anus

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Assessing the impact of drinking water iodine concentrations on the iodine intake of Chinese pregnant women living in areas with restricted iodized salt supply

European Journal of Nutrition (2020)

Min Gao W Chen Shangwen Dong Yue Chen Qiaozhi Zhang

Iodised salt

10.1007/s00394-020-02308-y

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Citations (8)

[Exogenous hydrogen sulfide inhibits high-glucose-induced injuries via regulating leptin/leptin receptor signaling pathway in human umbilical vein endothelial cells].

PubMed (2016)

Dong-Bo Wu Jingfu Chen Qing Xu Lin Jiaqiong Liao Jingqiu

To investigate whether exogenous hydrogen sulfide (H2S) inhibits the high-glucose (HG)-induced injury by modulating leptin/leptin receptor (LEPR) signal pathway in human umbilical vein endothelial cells (HUVECs).HUVECs were treated with 40 mmol/L glucose for 3-24 h, and the cell viability was examined by CCK-8 assay. The changes of cell morphology and the number of apoptotic cells were assessed by Hoechst 33258 nuclear staining followed by photofluorography. The intracellular levels of reactive oxygen species (ROS) was detected by DCFH-DA staining followed by photofluorography. Mitochondrial membrane potential (MMP) was determined by Rhodamine 123 (Rh123) staining and photofluorography. The expression levels of leptin and LEPR protein were measured by Western blotting.s The expression of leptin and LERP in HUVECs began to significantly increase at 3 h after HG exposure and reached the peak levels at 9 h (P<0.01). Pretreatment of HUVECs with 400 µmol/L sodium hydrosulfide (H2S donor) for 30 min inhibited HG-induced increase in leptin and leptin receptor expressions in HUVECs (P<0.01). Pretreatment of HUVECs with 400 µmol/L NaHS for 30 min or 50 ng/mL leptin antagonists (LA) for 1 h obviously alleviated HG-induced injury by increasing cell viability, decreasing cell apoptosis and lowering accumulation of intracellular ROS and MMP loss (P<0.01).Exogenous H2S protects against HG-induced injury by inhibiting leptin/LEPR pathway in HUVECs.

Sodium hydrosulfide

Viability assay

Leptin receptor

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An iASPP‐derived short peptide restores p53‐mediated cell death in cancers with wild‐type p53

iLABMED (2023)

Shi Qiu Qi Wei Wen Wu Qian Qiu Jiali Ma

Abstract Background Inhibitor of apoptosis‐stimulating protein of p53 (iASPP) is an evolutionarily conserved p53 inhibitor. Mechanistically, iASPP can accelerate tumorigenesis by inhibiting the transactivation function of p53. Targeting the interaction between iASPP and p53 may be a potential therapy for restoring the activity of p53 in tumors. Methods We constructed an iASPP‐derived peptide, called A8, that was derived from the C‐terminus of iASPP. Here, we transfected A8 into two wild‐type (WT) p53 cell lines, U2OS and A549, and then determined the number of apoptotic cells. The mechanism by which A8 affected apoptosis was further examined by immunoprecipitation (IP), Dual‐Luciferase reporter assays, and chromatin IP assays. Real‐time polymerase chain reaction and western blots were also used to examine the expression levels of apoptosis‐related factors. Results Our data demonstrate that A8 can increase apoptosis rates in WT p53 cell lines. Functional analysis suggested that A8 restored the transcriptional function and DNA binding activities of p53 toward the Bax and PUMA gene promoters. Moreover, A8 reduced cell proliferation and inhibited tumor growth in xenograft nude mice. Conclusions These data provide a new approach for restoring the tumor suppressor function of p53 in cancer cells that express WT p53 and therefore may serve as a novel cancer treatment strategy.

Chromatin immunoprecipitation

10.1002/ila2.21

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Citations (1)

Iodine Metabolism in Urine and Breast Milk among Lactating Women with Adequate Iodine

Journal of Nutrition (2024)

Shaohan Li Chun-xi Wang Yuangui Cheng Junjing Li Hexi Zhang

10.1016/j.tjnut.2024.04.041

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Saliva Iodine Concentration in Children and Its Association with Iodine Status and Thyroid Function

The Journal of Clinical Endocrinology & Metabolism (2020)

Wenxing Guo Ziyun Pan Ying Zhang Ya Jin Shuyao Dong

Abstract Context The effectiveness of saliva iodine concentration (SIC) in evaluating iodine status in children is not clear. Objective We aimed to explore associations between SIC and assessed indicators of iodine status and thyroid function. Design Cross-sectional study. Setting Primary schools in Shandong, China. Participants Local children aged 8 to 13 years with no known thyroid disease were recruited to this study. Main outcome measures Blood, saliva, and urine samples were collected to evaluate thyroid function and iodine status. Results SIC positively correlated with spot urinary iodine concentration (r = 0.29, P < 0.0001), 24-hour urinary iodine concentration (r = 0.35, P < 0.0001), and 24-hour urinary iodine excretion (r = 0.40, P < 0.0001). The prevalence of thyroid nodules (TN) and goiter showed an upward trend with SIC quantiles (P for trend < 0.05). Children with SIC <105 μg/L had a higher risk of insufficient iodine status (OR = 4.18; 95% CI, 2.67-6.56) compared with those with higher SIC. Those having SIC >273 μg/L were associated with greater risks of TN (OR = 2.70; 95% CI, 1.38-5.26) and excessive iodine status (OR = 18.56; 95% CI, 5.66-60.91) than those with lower SIC values. Conclusions There is a good correlation between SIC and urinary iodine concentrations. It is of significant reference value for the diagnosis of iodine deficiency with SIC of less than 105 μg/L and for the diagnosis of iodine excess and TN with SIC of more than 273 μg/L. Given the sanitary nature and convenience of saliva iodine collection, SIC is highly recommended as a good biomarker of recent iodine status in school-aged children.

10.1210/clinem/dgaa471

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Citations (18)

[Study on the relationship between Aurora A expression in cancer cell and treatment efficacy of taxol for prostate cancer].

PubMed (2007)

Wen Wu Yi Qu Xiao‐Kang Liu Lin Zhang Dezhi Mu

To explore the relationship between Aurora A expression and cell sensitivity to taxol, and elucidate the effect of Aurora A on the treatment efficacy of taxol for prostate cancer.A cell clone highly expressing Aurora A was constructed through transfected with full length Aurora A gene, and then termed as Du145-Aurora A. Cells were treated with different dose taxols. The growth inhibition rates of cells were detected by MTT assay, and the cell apoptosis rates were detected through flow cytometry. The growth inhibition and apoptosis rates of Du145-Aurora A and Du145 cells were compared for Aurora A up-regulated expression. Simultaneously, cells Du145-Aurora A were treated with DNAzymes for down-regulating the Aurora A expression, and then the variations of cell growth inhibited rate and apoptosis rate were detected.Up-regulation of Aurora A expression reduced the cell inhibition and apoptosis rates when treated by taxol, but the down-regulation of Aurora A expression after DNAzymes applied increased the cell inhibition and apoptosis rates when treated by taxol.There is a positive relationship between Aurora A expression and treatment efficacy of taxol for prostate cancer, and inhibiting Aurora A expression will enhance the treatment efficacy of taxol for prostate cancer.

DU145

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[Acute myeloid leukemia complicated with complex karyotypes and T-lymphoblastic lymphoma: a case report].

PubMed (2016)

Hongjuan Dong Wen Wu Jianhong Wang Hua-Feng Zhu Shan Gao

Lymphoblastic lymphoma

10.3760/cma.j.issn.0253-2727.2016.03.012

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Adverse Effects on the Thyroid of Chinese Pregnant Women Exposed to Long-Term Iodine Excess: Optimal and Safe Tolerable Upper Intake Levels of Iodine

Nutrients (2023)

Wen Wu Wenxing Guo Naifan Zhang Min Gao Kexin Zhang

Ensuring optimal iodine nutrition in pregnant women is a global public health concern. However, there is no direct data on safe tolerable upper intake levels (ULs) for pregnant women. A cross-sectional study was performed to determine the ULs of pregnant women. A total of 744 pregnant women were enrolled in this study. The median (IQR) urinary iodine concentration (UIC) in pregnant women was 150.2 (87.6, 268.0) μg/L, and the urinary iodine excretion (UIE) over 24 h was 204.2 (116.0, 387.0) μg/day. Compared with those with a UIE figure of between 150-250 μg/day, the reference group, the prevalence of thyroid dysfunction was 5.7 times higher (95%CI: 1.7, 19.2) in pregnant women with a UIE figure of between 450-550 μg/day, and 3.9 times higher (95%CI: 1.5, 10.3) in pregnant women with a UIE figure of ≥550 μg/day. Compared with an estimated iodine intake (EII) of between 100-200 μg/day, the reference group, the prevalence of thyroid dysfunction was 4.3 times higher (95%CI: 1.3, 14.4) in pregnant women with a UIE figure of between 500-600 μg/day, and 3.6 times higher (95%CI: 1.5, 8.9) in pregnant women with UIE of ≥600 μg/day. In general, our cross-sectional study found that excessive iodine intake during pregnancy appears to directly increase the risk of thyroid dysfunction. Avoiding chronic iodine intakes of 500 μg/day or higher or having a UIE figure of ≥450 μg/day is recommended for pregnant women in China.

Cross-sectional study

10.3390/nu15071635

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Citations (3)

[Two cases of pulmonary Schizophyllum commune infection and literature review].

PubMed (2021)

Y F Wang Y X Zhang Yiming Zeng Wen Wu Y F Chen

本文分析2例肺部普通裂褶菌感染患者的临床资料，同时进行文献复习，总结肺部普通裂褶菌感染的临床表现、影像学特点和治疗方案等，以提高临床医生对该病的诊治水平。本组2例肺部普通裂褶菌感染者主要表现为咳嗽、咳痰，胸部CT影像学特点为肺部实变影、部分痰栓形成及肺部团块影，其中例1患者经支气管镜镜下清除痰栓，肺部病灶完全吸收。对有腐木等可疑接触史，伴有呼吸道症状，实验室检查示外周血嗜酸性粒细胞计数、总IgE水平升高，胸部影像学提示有痰栓改变，应考虑到该病的可能，积极完善支气管镜检查及病原学送检是关键。.

Schizophyllum commune

Pulmonary infection

10.3760/cma.j.cn112138-20210113-00033

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