Zi‐Sheng Zhang

Hefei National Center for Physical Sciences at Nanoscale

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Cheng‐Bin Xiang

Hefei National Center for Physical Sciences at Nanoscale

Jin‐Qiu Xia

Hefei National Center for Physical Sciences at Nanoscale

Jie Wu

University of Chicago

Liangqi Sun

University of Science and Technology of China

Ying Song

Fujian Institute of Research on the Structure of Matter

Lin‐Hui Yu

Brookhaven National Laboratory

Alamin Alfatih

Hefei National Center for Physical Sciences at Nanoscale

Ping‐Xia Zhao

Hefei National Center for Physical Sciences at Nanoscale

Zheng‐Yi Zhang

Hefei National Center for Physical Sciences at Nanoscale

Guang-Yu Wan

University of Science and Technology of China

Cooperative Institutions

Chinese Academy of Sciences

University of Science and Technology of China

Hefei National Center for Physical Sciences at Nanoscale

National Science Center

Rice Research Institute

Sichuan Agricultural University

Lanzhou University

University of Chinese Academy of Sciences

Central South University

Ministry of Agriculture and Rural Affairs

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Loss of ricePARAQUAT TOLERANCE 3confers enhanced resistance to abiotic stresses and increases grain yield in field

bioRxiv (Cold Spring Harbor Laboratory) (2020)

Alamin Alfatih Jiu Hui Wu Sami Ullah Jan Zi‐Sheng Zhang Jing‐Qiu Xia

ABSTRACT Plants frequently suffer from environmental stresses in nature and have evolved sophisticated and efficient mechanisms to cope with the stresses. To balance between growth and stress response, plants are equipped with efficient means to switch off the activated stress responses when stresses diminish. We previously revealed such an off-switch mechanism conferred by Arabidopsis PQT3 , knockout of which significantly enhances resistance to abiotic stresses. To explore whether the rice homolog OsPQT3 is functionally conserved, we generated three knockout mutants with CRISPR-Cas9 technology. The OsPQT3 knockout mutants ( ospqt3 ) display enhanced resistance to oxidative and salt stress with elevated expression of OsGPX1 , OsAPX1 , and OsSOD1 . More importantly, the ospqt3 mutants show significantly enhanced agronomic performance with higher yield compared with the wild type under salt stress in greenhouse as well as in field conditions. We further showed that OsPQT3 was rapidly down regulated in response to oxidative and other abiotic stresses as AtPQT3 . Taken together, these results support our previous findings that AtPQT3 acts as an off-switch in stress response, which is well conserved in rice. Therefore, PQT3 locus provides a promising candidate for crop improvement with enhanced stress resistance by gene editing technology.

10.1101/2020.02.22.961151

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Citations (3)

Loss ofOsARF18confers glufosinate ammonium herbicide resistance in rice

bioRxiv (Cold Spring Harbor Laboratory) (2023)

Jin‐Qiu Xia Da‐Yu He Qin‐Yu Liang Zheng‐Yi Zhang Jie Wu

ABSTRACT Weed is one of the major biotic stresses that causes severe loss of crop yield. Herbicide is one of the most cost-effective ways to control weeds. Thus, the development of herbicide-resistant crops is critical for the application of herbicides. To isolate new glufosinate ammonium resistance loci, we screened a rice ethyl methyl sulfonate-mutagenized library and obtained the g lufosinate a mmonium- r esistant mutant gar1-1 . GAR1 encodes auxin response factor 18 ( OsARF18 ). A G-to-A substitution in the coding region of OsARF18 results in loss of function of OsARF18 and thereby enhances glufosinate ammonium resistance of gar1-1 , which was confirmed by three additional CRISPR/Cas9-edited gar1 alleles. GLUTAMINE SYNTHETASE 1;1 ( OsGS1;1 ) and GLUTAMINE SYNTHETASE 1;2 ( OsGS1;2 ) were upregulated in gar1-1 upon glufosinate ammonium treatment, directly contributing to increased GS activity that enhances glufosinate ammonium herbicide resistance. We further show that OsARF18 suppresses OsGS1;1 and OsGS1;2 expression. Comparative transcriptomic analyses reveal a huge shift in the gene expression profile involved in stress tolerance and growth. A large number of detoxification-related genes are enriched in gar1-1 , which may also contribute to enhanced herbicide resistance. Moreover, stress tolerance-related genes are upregulated and growth-related genes are downregulated in gar1-1 , consistent with the improved tolerance to salt and osmotic stress of gar1 mutants. Taken together, our study demonstrates that OsARF18 is a negative regulator of glufosinate ammonium resistance as well as salt and osmotic stress tolerance, suggesting a role in balancing the stress response and growth.

Glufosinate

Wild type

10.1101/2023.06.05.543806

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Rice NIN‐LIKE PROTEIN 4 plays a pivotal role in nitrogen use efficiency

Plant Biotechnology Journal (2020)

Jie Wu Zi‐Sheng Zhang Jin‐Qiu Xia Alamin Alfatih Ying Song

Summary Nitrogen (N) is one of the key essential macronutrients that affects rice growth and yield. Inorganic N fertilizers are excessively used to boost yield and generate serious collateral environmental pollution. Therefore, improving crop N use efficiency (NUE) is highly desirable and has been a major endeavour in crop improvement. However, only a few regulators have been identified that can be used to improve NUE in rice to date. Here we show that the rice NIN‐like protein 4 (OsNLP4) significantly improves the rice NUE and yield. Field trials consistently showed that loss‐of‐ OsNLP4 dramatically reduced yield and NUE compared with wild type under different N regimes. In contrast, the OsNLP4 overexpression lines remarkably increased yield by 30% and NUE by 47% under moderate N level compared with wild type. Transcriptomic analyses revealed that OsNLP4 orchestrates the expression of a majority of known N uptake, assimilation and signalling genes by directly binding to the nitrate‐responsive cis ‐element in their promoters to regulate their expression. Moreover, overexpression of OsNLP4 can recover the phenotype of Arabidopsis nlp7 mutant and enhance its biomass. Our results demonstrate that OsNLP4 plays a pivotal role in rice NUE and sheds light on crop NUE improvement.

10.1111/pbi.13475

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Citations (95)

Rice NIN‐LIKE PROTEIN 3 modulates nitrogen use efficiency and grain yield under nitrate‐sufficient conditions

Plant Cell & Environment (2022)

Zi‐Sheng Zhang Jin‐Qiu Xia Alamin Alfatih Ying Song Yi‐Jie Huang

Abstract Nitrogen (N) is an essential macronutrient for crop growth and yield. Improving the N use efficiency (NUE) of crops is important to agriculture. However, the molecular mechanisms underlying NUE regulation remain largely elusive. Here we report that the OsNLP3 (NIN‐like protein 3) regulates NUE and grain yield in rice under N sufficient conditions. OsNLP3 transcript level is significantly induced by N starvation and its protein nucleocytosolic shuttling is specifically regulated by nitrate. Loss‐of‐function of OsNLP3 reduces plant growth, grain yield, and NUE under sufficient nitrate conditions, whereas under low nitrate or different ammonium conditions, osnlp3 mutants show no clear difference from the wild type. Importantly, under sufficient N conditions in the field, OsNLP3 overexpression lines display improved grain yield and NUE compared with the wild type. OsNLP3 orchestrates the expression of multiple N uptake and assimilation genes by directly binding to the nitrate‐responsive cis ‐elements in their promoters. Overall, our study demonstrates that OsNLP3, together with OsNLP1 and OsNLP4, plays overlapping and differential roles in N acquisition and NUE, and modulates NUE and the grain yield increase promoted by N fertilizer. Therefore, OsNLP3 is a promising candidate gene for the genetic improvement of grain yield and NUE in rice.

10.1111/pce.14294

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Citations (41)

Ternary liquid-liquid equilibria of water, ethanol, and oleic acid. [Erratum to document cited in CA115(16):167680b]

Journal of Chemical & Engineering Data (1992)

Zi‐Sheng Zhang Gordon A. Hill

ADVERTISEMENT RETURN TO ISSUEPREVArticleTernary liquid-liquid equilibria of water, ethanol, and oleic acid. [Erratum to document cited in CA115(16):167680b]Zisheng Zhang and Gordon A. HillCite this: J. Chem. Eng. Data 1992, 37, 2, 284Publication Date (Print):April 1, 1992Publication History Published online1 May 2002Published inissue 1 April 1992https://pubs.acs.org/doi/10.1021/je00006a040https://doi.org/10.1021/je00006a040research-articleACS PublicationsRequest reuse permissionsArticle Views45Altmetric-Citations-LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts

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10.1021/je00006a040

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ERF1 inhibits lateral root emergence by promoting local auxin accumulation with altered distribution and repressingARF7expression

bioRxiv (Cold Spring Harbor Laboratory) (2023)

Ping‐Xia Zhao Jing Zhang Siyan Chen Zi‐Sheng Zhang Guang-Yu Wan

Summary Lateral roots (LRs) are crucial for plants to sense environmental signals in addition to water and nutrient absorption. Auxin is key for LR formation, but the underlying mechanisms are not fully understood. Here we report that Arabidopsis ERF1 inhibits LR emergence by promoting local auxin accumulation with altered distribution and regulating auxin signaling. Loss of ERF1 increases LR density compared with the wild type, whereas ERF1 overexpression causes the opposite phenotype. ERF1 enhances auxin transport by upregulating PIN1 and AUX1 , resulting in excessive auxin accumulation in the endodermal, cortical, and epidermal cells surrounding LR primordia. Furthermore, ERF1 represses ARF7 transcription, consequently affecting the expression of cell wall remodeling genes that facilitate LR emergence. Together, our study reveals that ERF1 integrates environmental signals to promote local auxin accumulation with altered distribution and repress ARF7 , consequently inhibiting LR emergence in adaptation to fluctuating environments. Highlights ERF1 functions as a negative regulator of lateral root emergence ERF1 enhances rootward and shootward auxin transport by directly upregulating the expression of PIN1 and AUX1 , resulting in high local auxin accumulation and abnormal auxin distribution in the endodermal, cortical, and epidermal cells overlying lateral root primordia ERF1 represses the transcription of ARF7 and cell wall remodeling genes in lateral root emergence

Primordium

Polar auxin transport

Lateral root

10.1101/2023.03.02.530895

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Citations (2)

Arabidopsis MADS-Box Transcription Factor AGL21 Acts as Environmental Surveillance of Seed Germination by Regulating ABI5 Expression

Molecular Plant (2017)

Lin‐Hui Yu Jie Wu Zi‐Sheng Zhang Zi-Qing Miao Ping‐Xia Zhao

MADS-box

Agamous

Chromatin immunoprecipitation