The objective of this study was to analyze the efficacy of chewing function nursing techniques in improving swallowing dysfunction among children with cerebral palsy. A total of 60 children with cerebral palsy and swallowing disorders, admitted to our hospital from January 2020 to December 2022, were included in the study. The children were randomly divided into an observation group and a control group using a random number table. The control group received routine rehabilitation nursing, while the observation group received additional nursing measures focusing on chewing function techniques in addition to the routine rehabilitation nursing. Both groups underwent interventions for a period of 3 months. Swallowing dysfunction, eating ability, and oral motor function were evaluated and compared between the two groups before and after treatment. Specifically, the Dysphagia Diet Scale (DDS) was used to assess swallowing dysfunction, the Eating and Drinking Ability Classification System (EDACS) was used to evaluate eating ability, and the Structured Oral Motor Assessment (SOMA) was used to assess oral motor function. The results showed that before rehabilitation nursing, there was no significant difference in DDS scores between the two groups. However, after rehabilitation nursing, both groups exhibited lower DDS scores compared to before, and the observation group had lower scores than the control group, indicating a significant difference (P < 0.05). Similarly, before rehabilitation nursing, there was no significant difference in EDACS ratings between the two groups. After rehabilitation nursing, both groups of children showed improvement in EDACS ratings, with the observation group demonstrating better outcomes than the control group. The difference between the two groups was significant (P < 0.05). Prior to rehabilitation nursing, there was no significant difference in SOMA scores between the two groups (P > 0.05). However, after rehabilitation nursing, both groups exhibited improved SOMA scores, with the observation group scoring higher than the control group. The difference between the two groups was also significant (P < 0.05). In conclusion, compared to routine nursing care, chewing function nursing techniques can alleviate swallowing dysfunction among children with cerebral palsy, enhance their swallowing function, and improve their quality of life. Therefore, this approach is worthy of clinical application and promotion.
In order to adapt to the characteristics of high mobility and high efficiency firing of aviation artillery shells, meet the requirements of convenient transfer and rapid formation of security capabilities, the design of an aerial gun pressure and discharge machine is optimized by using the bullet chain supply structure, and the two working processes of pressure and discharge are realized through the mode of interchangeable components, and the key motion process is simulated and verified by using the dynamics software ADAMS. The power on its power shaft is about 130N,comply with index design requirements. The motion process is reliable. The structure has the characteristics of small size, light weight, simple operation, fast discharge speed, stable and reliable quality, easy to carry and transfer, etc., which can meet the needs of high-intensity modern warfare.
A major and critical virulence determinant of many Gram-negative bacterial pathogens is the Type III Secretion Systems (T3SS). T3SS3 in Burkholderia pseudomallei is critical for bacterial virulence in mammalian infection models but its regulation is unknown. B. pseudomallei is the causative agent of melioidosis, a potentially fatal disease endemic in Southeast Asia and northern Australia. While screening for bacterial transposon mutants with a defective T3SS function, we discovered a TetR family regulator (bspR) responsible for the control of T3SS3 gene expression. The bspR mutant exhibited significant virulence attenuation in mice. BspR acts through BprP, a novel transmembrane regulator located adjacent to the currently delineated T3SS3 region. BprP in turn regulates the expression of structural and secretion components of T3SS3 and the AraC family regulator bsaN. BsaN and BicA likely form a complex to regulate the expression of T3SS3 effectors and other regulators which in turn affect the expression of Type VI Secretion Systems (T6SS). The complete delineation of the bspR initiated T3SS regulatory cascade not only contributes to the understanding of B. pseudomallei pathogenesis but also provides an important example of how bacterial pathogens could co-opt and integrate various regulatory motifs to form a new regulatory network adapted for its own purposes.
Modified radical mastectomy (MRM) is the most common surgical treatment for breast cancer. General anesthesia poses a challenge in fragile MRM patients, including cardiovascular instability, insufficient postoperative pain control, nausea and vomiting. Thoracic paravertebral block (TPVB) is adequate for simple mastectomy, but its combination with interscalene brachial plexus block (IBPB) has not yet been proved to be an effective anesthesia method for MRM.We describe our experience of anesthesia and pain management in 10 patients with multiple comorbidities. An ultrasound-guided TPVB was placed at T2-T3 and T5-T6, and combined with IBPB, with administration of 10, 15 and 5 mL of 0.5% ropivacaine, respectively. A satisfactory anesthetic effect was proved by the absence of ipsilateral tactile sensation within 30 min. Propofol 3 mg/kg/h and oxygen supplementation via a nasal cannula were administered during surgery. None of the patients required additional narcotics, vasopressors, or conversion to general anesthesia. The maximum pain score was 2 on an 11-point numerical rating scale. Two patients required one dose of celecoxib 8 h postoperatively and none reported nausea or emesis.This case series demonstrated that combined two-site TPVB and small-volume IBPB with sedation can be used as an alternative anesthetic modality for MRM, providing good postoperative analgesia.
Effects of nitrogen and sulphur [N3.75S0.35 (N 3.75 mmol/L, S 0.35mmol/L), N3.75S4.2(N 3.75 mmol/L, S 4.2mmol/L), N7.5S1.4(N 7.5 mmol/L, S 1.4mmol/L), N22.5S0.35(N 22.5 mmol/L, S 0.35mmol/L)] on the growth and quality of in bunching onion (Allium fistulosum L. var. caespeitosum Makino) were studied in hydroponics, in which the solution was Hoagland's. The results showed that the growth of bunching onion in N7.5S1.4 treatment was better than in other treatments. With nitrogen increasing, the growth of bunching onion enhanced at the same sulphur level, and the growth of bunching onion enhanced by sulphur increase at the same nitrogen level. Concentrations of chlorophyll a, b, total chlorophyll and carotenoid in N7.5S1.4 treatment were highest. With nitrogen and sulphur increasing, concentration of soluble protein and nitrate in bunching onion increased. While concentration of soluble sugar decreased with nitrogen increased.
Melioidosis is a life-threatening bacterial infection caused by Burkholderia pseudomallei. Some antibiotics used to treat melioidosis can induce filamentation in B. pseudomallei. Despite studies on the mechanism of virulence of the bacteria, the properties of B. pseudomallei filaments and their impact on virulence have not been investigated before. To understand the characteristics of antibiotic-induced filaments, we performed in vitro assays to compare several aspects of virulence between normal, nonfilamentous and filamentous B. pseudomallei. Normal, nonfilamentous B. pseudomallei could cause the lysis of monocytic cells, while filaments induced by sublethal concentrations of ceftazidime, ofloxacin, or trimethoprim show decreased lysis of monocytic cells, especially after prolonged antibiotic exposure. The motility of the filamentous bacteria was reduced compared to that of nonfilamentous bacteria. However, the filamentation was reversible when the antibiotics were removed, and the revertant bacteria recovered their motility and ability to lyse monocytic cells. Meanwhile, antibiotic resistance developed in revertant bacteria exposed to ceftazidime at the MIC. Our study highlights the danger of letting antibiotic concentration drop to the MIC or sub-MICs during antibiotic treatment of melioidosis. This could potentially give rise to a temporary reduction of bacterial virulence, only to result in bacteria that are equally virulent but more resistant to antibiotics, should the antibiotics be reduced or removed.
Effect of ammonium and nitrate ratio (0:100, 25:75, 50:50 and 75:25) on nutritional quality of Chinese kale (Brassica alboglabra Bailey) with 3 cultivars were studied in hydroponics. The results indicated that, the Vitamin C content in product organ (leaf and stalk) of Chinese kale was decreased by the enhancement of ammonium in nutrient solution, and the decrease was not significant for 25% enhancement of ammonium. 25% ammonium enhancement increased the soluble sugar content in product organ significantly, compared with other treatments. Free amino acids and protein content of Chinese kale increased gradually with the increasing of ammonium proportion in nutrient solution. Integrated nutritional quality of Chinese kale in 25% ammonium enhancement treatment was better than others under hydroponics condition.
In view of the dynamics of the dam safety monitoring data, the sensitivity to time and space, and the nonlinearity, it has been proposed to use the firefly algorithm to search to determine the delay order and the number of hidden layer units and combine them with nonlinear autoregressive algorithms. The algorithms are combined to obtain the FA-NAR algorithm dam deformation prediction model, which is compared with the traditional BP algorithm prediction results, combined with the Xiaolangdi dam deformation monitoring data for prediction, and the dam deformation data predicted by the dynamic neural network have a better convergence effect and a more accurate prediction result. It provides a certain reference basis for perfecting dam safety monitoring.
Fish diversity, an important indicator of the health of aquatic ecosystems, is declining sharply due to water pollution, overfishing, climate change, and species invasion. Effective surveying and monitoring are prerequisites for protecting fish diversity. In this study, eDNA (environmental DNA）metabarcoding and ground cages were used to survey the fish diversity of the Chaobai and Beiyun Rivers in Beijing. Based on the two methods, the results showed that 40 species, belonging to 35 genera, 18 families, and six orders, were identified. The richness of fish identified by eDNA metabarcoding was higher than that captured by ground cages in both rivers. All fish captured by the ground cage method were recognized by eDNA metabarcoding, except Squalidus wolterstorffi and Saurogobio dabryi, which were captured only in ground cages. The correlation of relative abundance between the two methods was affected by the properties of the rivers, such as the flow rate. The relative abundance detected by eDNA metabarcoding was positively correlated with that of the ground cages in the Beiyun River, while no correlation was observed for the Chaobai River. Our results also suggest that the Chaobai River has higher fish diversity and a different population structure than the Beiyun River. The primary fish species of the Beiyun River are mainly pollution resistant. This results show that eDNA metabarcoding as a new survey tool has great application prospects. It provides certain theoretical data and methodological references for the protection and management of river fish diversity.
Human kinesin family member 11 (KIF11) plays a vital role in regulating the cell cycle and is implicated in the tumorigenesis and progression of various cancers, but its role in endometrial cancer (EC) is still unclear. Our current research explored the prognostic value, biological function and targeting strategy of KIF11 in EC through approaches including bioinformatics, machine learning and experimental studies. The GSE17025 dataset from the GEO database was analyzed via the limma package to identify differentially expressed genes (DEGs) in EC. Functional enrichment analysis of the DEGs was conducted using Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses. DEGs were further screened for hub genes through protein–protein interaction (PPI) network analysis and machine learning. The role of the hub gene KIF11 in EC was analyzed using clinical data from the TCGA database. The expression of KIF11 in EC was subsequently validated in clinical samples. In vitro experiments were utilized to evaluate the effects of KIF11 on biological functions such as proliferation, migration, apoptosis, and the cell cycle in endometrial cancer cells. A total of 877 DEGs, which are widely involved in important biological processes such as cell division, tubulin binding, and the cell cycle, were identified. Through PPI network analysis and machine learning, KIF11 was selected as the hub gene for subsequent analysis and experimental validation. An analysis of TCGA data revealed that KIF11 is highly expressed in EC and is associated with tumor grade, stage, and a low survival rate. The overexpression of KIF11 in tumor tissues was further confirmed in EC patient samples. KIF11 knockdown had inhibitory effects on cell proliferation, migration and invasion. Flow cytometry analysis revealed that KIF11 knockdown induced G2/M phase arrest and promoted apoptosis in EC cells. Our study demonstrated that KIF11 was upregulated in EC and was strongly associated with a poor prognosis. Notably, we found that reduced KIF11 expression inhibited EC cell proliferation, migration and invasion. KIF11 knockdown caused more EC cells to arrest in the G2/M phase and undergo apoptosis. The findings of our study emphasized that KIF11 may be a promising prognostic biomarker and therapeutic target for EC patients.
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