Donglin Guo

Hunan University of Arts and Science

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Yongjun Shu

Harbin Normal University

Changhong Guo

Guangdong University of Technology

Yan Bai

Zhejiang A & F University

Xueqi Zhang

Qinghai University

Manman Li

Northwest A&F University

Chao Chen

Harbin Normal University

Jilin Li

Second Affiliated Hospital of Shantou University Medical College

Changhong Guo

Harbin Normal University

Ning Wang

University of Sheffield

Huanhuan Yang

Northeast Agricultural University

Cooperative Institutions

Harbin Normal University

Northeast Agricultural University

Education Department of Heilongjiang Province

Wuhan University

Chinese Academy of Medical Sciences & Peking Union Medical College

University of Science and Technology of China

Tongji University

Shanghai University

Ministry of Agriculture and Rural Affairs

Northeast Forestry University

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A Pan-Transcriptome Analysis Indicates Efficient Downregulation of the FIB Genes Plays a Critical Role in the Response of Alfalfa to Cold Stress

Plants (2022)

Xueqi Zhang Huanhuan Yang Manman Li Yan Bai Chao Chen

Alfalfa (Medicago sativa L.) is a perennial forage legume that is widely distributed throughout the world, and cold stress is an important environmental factor limiting the growth and production of alfalfa in cold regions. However, little is known of the molecular mechanisms regarding cold tolerance in alfalfa. Here, we conducted physiological metabolism assays and pan-transcriptome sequencing on eight cultivars of alfalfa under cold stress conditions. The results of the RNA-seq analysis showed that the genes are “oxidoreductase activity” and “transcription regulator activity”, suggesting that genes with such functions are more likely to play important roles in the response to cold stress by alfalfa. In addition, to identify specific gene modules and hub genes in response to alfalfa cold stress, we applied weighted gene co-expression network (WGCNA) analyses to the RNA-seq data. Our results indicate that the modules of genes that focus on the ATPase complex, ribosome biogenesis, are more likely to be involved in the alfalfa response to cold stress. It is important to note that we identified two fibronectin (FIB) genes as hub genes in alfalfa in response to cold stress and that they negatively regulate alfalfa response to chilling stress, and it is possible that dormant alfalfa is more effective at down-regulating FIB expression and therefore more resistant to cold stress.

RNA-Seq

10.3390/plants11223148

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Citations (12)

Comprehensive Identification of the β-Amylase (BAM) Gene Family in Response to Cold Stress in White Clover

Plants (2024)

Manman Li Xiuhua Chen Wangqi Huang Kaiyue Wu Yan Bai

White clover (

White (mutation)

Identification

Cold stress

10.3390/plants13020154

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Citations (3)

Time-course RNA-seq analysis provides an improved understanding of genetic regulation in response to cold stress from white clover (Trifolium repens L.)

Biotechnology & Biotechnological Equipment (2022)

Xueqi Zhang Huanhuan Yang Manman Li Chao Chen Yan Bai

White clover (Trifolium repens L.) is an important legume forage, which is widely distributed in cool-season regions. Therefore, cold stress is a major environmental factor limiting its growth and production, while little is known about the its cold tolerance at molecular level. In the present study, we performed time-course RNA-seq analysis under cold stress. RNA-seq results suggested that genes associated with "oxidoreductase activity" and "transcription regulator activity" are more likely related to the response of white clover to cold stress. To identify the specific gene modules and the hub genes of white clover in response to cold stress, we applied weighted gene co-expression network analyses (WGCNA) to transcriptome data. We also found that gene modules that focus on protein kinase activity, DNA-binding transcription factor activity and oxidoreductase activity, are more likely to be involved in the response of white clover to cold stress. Especially, we identified several AP2/ERF TF genes and CDPK genes as pivotal genes in white clover in response to cold stress, which would provide helpful insights into the molecular mechanisms underlying the response of white clover to cold stress.

Trifolium repens

RNA-Seq

White (mutation)

Transcription

Cold stress

10.1080/13102818.2022.2108339

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Citations (9)

MICRODISSECTION OF CHROMOSOME 1B OF TRITICUM SPELTA VAR. DUHAMELIANCUM AND AMPLIFICATION OF THE CHROMOSOME DNA

Zhiwu yanjiu (2002)

Donglin Guo Wang Tong-cang Ning Wang Shuhong Xu Jilin Li

Microdissection

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Genome-Wide Analysis of Oligopeptide Transporters Gene Family in <em>Medicago truncatula</em>

Renze Liu Hongyu Shan Yongjun Shu Changhong Guo Donglin Guo

Oligopeptide transporters (OPTs) are a group of membrane localized proteins that have a broad range of substrate transport capabilities and contribute to numerous biological processes. However, limited information has been reported on OPTs in higher plants. In this study, a comprehensive analysis of the OPT gene family in Medicago truncatula was performed. A total of 26 OPT genes (MtOPT01-MtOPT26) have been identified in the Medicago truncatula genome. Phylogenetic analyses indicated that MtOPTs consisted of two distinct subgroups, 12 MtOPTs belonged to the peptide transport subgroup (PT-OPT) based on their predicted amino acid sequences containing the two highly conserved motifs (NPG and KIPPR) and 14 MtOPTs belonged to yellow stripe subgroup (YS-OPT). The MtOPTs distributed on each of 8 chromosomes in Medicago truncatula. Sequence analysis verified that MtOPTs significant similar to those in other plants. The copy number of MtOPTs was low and the multiply of MtOPTs was simple relatively. Gene structure analysis showed that most of the MtOPTs have various numbers of introns. The multiple of MtOPTs might play different biological roles which were supported by the fact that MtOPTs have a distinct tissue-specific expression pattern. The data obtained in this study will help to better understand the complexity of the MtOPTs gene family and provide new evidence for the function and evolution of the OPT gene family in higher plants.

Medicago truncatula

Oligopeptide

10.20944/preprints201609.0048.v1

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Citations (1)

Application of music therapy during the COVID-19

Advances in Social Behavior Research (2024)

Donglin Guo

The sudden attack of the new crown pneumonia epidemic, the rapid spread of the virus, has caused a severe impact on the psychology of medical staff, patients and ordinary people. In order to reduce the negative impact of the epidemic on the people's psychology, China has begun to provide professional music mental health services for different groups of people to reduce the psychological harm caused by the epidemic. Chinese music therapists actively participated in epidemic prevention operations and launched a number of "fighting the epidemic-psychological assistance for music therapy" programs. According to people's emotional and psychological adjustment needs during the epidemic, the project carried out music psychological public welfare activities for various key groups. Activities include music listening, music relaxation, music imagination, music painting, song discussion, song lyrics, body rhythm, etc., from listening and experiencing music to participating music interaction and other rich group music psychological activities. Through music experience and interaction, the activity relieves negative emotions such as anxiety and depression, increases positive and pleasant emotions, promotes communication and interaction among participants, reduces loneliness caused by isolation, and promotes family harmony.

Music Therapy

Lyrics

10.54254/2753-7102/6/2024049

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MICRODISSECTION AND AMPLIFICATION IN VITRO OF CHROMOSOME 1R IN RYE (Secale cereale L.) AND DETERMINATION OF THE AMPLIFIED PRODUCTS

Zhiwu yanjiu (2000)

Wei Ji-cheng Rui Shi Changhong Guo Donglin Guo MA Xu-jun

The chromosome 1R of rye was microdissected and isolate d under reverse -microscope with a manipulator from the cells of root tip at mitotic metaphase. Single chromosome 1R was then transferred into 0.5 ml centrifuge tube. After the treatment with Protease K, the DNA of chromosome 1R was digested with restric tion enzyme Sau3A, then the Sau3A linker-adaptor was ligated to the ends of the DNA fragments, which was subsequently amplified by means of linker-adapter polym erase chain reaction (LA-PCR) with one of chains of the linker-adapter as primer . By dot blotting with biotinlatyled rDNA of wheat and genome DNA of rye as prob es, it was confirmed that the amplified products by LA-PCR are derived from the DNA of chromosome 1R in rye. This work would facilitated to construct the sub-ge nomic DNA library of chromosome 1R and to screen the specific probes of chromoso me 1R in rye.

Adapter (computing)

Secale

Primer (cosmetics)

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Citations (1)

Application of music therapy during the COVID-19

Advances in Social Behavior Research (2024)

Donglin Guo

Music Therapy

Lyrics

10.54254/2753-7102/7/2024049