Film clips are widely used in emotion research due to their relatively high ecological validity. Although researchers have established various film clip sets for different cultures, the few that exist related to Chinese culture do not adequately address positive emotions. The main purposes of the present study were to establish a standardised database of Chinese emotional film clips that could elicit more categories of reported positive emotions compared to the existing databases and to expand the available film clips that can be used as neutral materials. Two experiments were conducted to construct the database. In experiment 1, 111 film clips were selected from more than one thousand Chinese movies for preliminary screening. After 315 participants viewed and evaluated these film clips, 39 excerpts were selected for further validation. In experiment 2, 147 participants watched and rated these 39 film clips, as well as another 8 excerpts chosen from the existing databases, to compare their validity. Eventually, 22 film excerpts that successfully evoked three positive emotions (joy, amusement, and tenderness), four negative emotions (moral disgust, anger, fear, and sadness), and neutrality formed the standardised database of Chinese emotional film clips.
<p>PDF file - 362K, Competitive binding assay (S1); TPL inhibits ErbB2 signaling in breast cancer cell lines (S2); ErbB2 knockdown inhibits ErbB2 signaling and results in growth inhibition in the MDA-MB-231 cell line (S3); TPL inhibited MDA-MB-231 breast cancer cell growth in a dose-dependent manner both in vitro and in vivo (S4); The effects of siRNA knockdown of EGFR or ErbB3 on the BT-474TraR cell line (S5).</p>
An increasing number of foreign companies want to enter the Chinese market. Online advertising is one of the indispensable means of the company's operation and marketing, and foreign companies poor ...
<p>PDF file - 150K, Relative binding affinity of anti-ErbB2 antibodies for the trastuzumab epitope on ErbB2 in BT-474 cells (S1); Relative binding affinity of anti-ErbB2 antibodies for the pertuzumab epitope on ErbB2 in BT-474 cells (S2); Binding affinities of recombinant anti-ErbB2 antibodies for ErbB2-ECD (S3); Pharmacokinetic parameters of recombinant anti-ErbB2 antibodies in mice (S4); ErbB receptor expression on breast cancer cell lines (S5).</p>
<p>PDF file - 362K, Competitive binding assay (S1); TPL inhibits ErbB2 signaling in breast cancer cell lines (S2); ErbB2 knockdown inhibits ErbB2 signaling and results in growth inhibition in the MDA-MB-231 cell line (S3); TPL inhibited MDA-MB-231 breast cancer cell growth in a dose-dependent manner both in vitro and in vivo (S4); The effects of siRNA knockdown of EGFR or ErbB3 on the BT-474TraR cell line (S5).</p>
Traditional Chinese medicines must be processed into decoction pieces before they can be used medicinally, and decoctions are the most common dosage form in TCM. Recent studies have shown that these decoctions are complex multiphase disperse systems containing supramolecular aggregates. These aggregates form through self-assembly interactions among chemical components du-ring the decocting process, in which hydrogen bonding, π-π interactions, van der Waals' forces, and hydrophobic interactions playing crucial roles. Exploring supramolecular aggregates in traditional Chinese medicine decoctions can help address the limitations of a single-component perspective in understanding the medicinal substances, thus becoming a research hotspot. This paper presents a comprehensive overview of the methods for the separation and purification, structure characterization, and self-assembly mechanism revealing of these aggregates and summarizes the self-assembly mechanisms and chemical components of these aggregates. By examining several examples of supramolecular aggregates in traditional Chinese medicine decoctions and assessing processing adjuvants, this paper proposes a new idea of analyzing the processing mechanism based on the formation of supramolecular aggregates. This idea combines the characteristics of traditional Chinese medicine processing with medicine compatibility principles to improve the understanding about the scientific connotation of processing with adjuvants.
Enhancing the dissolution, permeation and absorption of active components with low solubility and poor permeability is crucial for maximizing therapeutic efficacy and optimizing functionality. The objective of this study is to investigate the potential of natural polysaccharides as carriers to improve the biopharmaceutical properties of active components.In this study, we employed four representative flavonoids in Astragali Radix, namely Calycosin-7-O-β-D-glucoside (CAG), Ononin (ON), Calycosin (CA) and Formononetin (FMN), as a demonstration to evaluate the potential of Astragalus polysaccharides (APS) as carriers to improve the biopharmaceutical properties, sush as solubility, permeability, and absorption in vivo. In addition, the microstructure of the flavonoids-APS complexes was characterized, and the interaction mechanism between APS and flavonoids was investigated using multispectral technique and molecular dynamics simulation.The results showed that APS can self-assemble into aggregates with a porous structure and large surface area in aqueous solutions. These aggregates can be loaded with flavonoids through weak intermolecular interactions, such as hydrogen bonding, thereby improving their gastrointestinal stability, solubility, permeability and absorption in vivo.We discovered the self-assembly properties of APS and its potential as carriers. Compared with introducing external excipients, the utilization of natural polysaccharides in plants as carriers may have a unique advantage in enhancing dissolution, permeation and absorption.
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