Pingping Lv

Ningbo University

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Jian‐Zhong Sheng

Women's Hospital, School of Medicine, Zhejiang University

Chun Feng

Huazhong University of Science and Technology

Guolian Ding

Maternal and Child Health Hospital of Xinjiang Uygur Autonomous Region

Hefeng Huang

Women's Hospital, School of Medicine, Zhejiang University

Dan Zhang

Women's Hospital, School of Medicine, Zhejiang University

He‐Feng Huang

Zhejiang University

Min Jin

Union Hospital

Gufeng Xu

Beijing United Family Hospital

Y. Ying

Women's Hospital, School of Medicine, Zhejiang University

M. Ye

Chinese Academy of Agricultural Sciences

Cooperative Institutions

Zhejiang University

125

Women's Hospital, School of Medicine, Zhejiang University

Chinese Academy of Sciences

Shanghai Jiao Tong University

Ministry of Education of the People's Republic of China

Chinese Academy of Medical Sciences & Peking Union Medical College

University of Chinese Academy of Sciences

Second Affiliated Hospital of Zhejiang University

Fudan University

International Peace Maternity & Child Health Hospital

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Active Ingredients of Schisandra chinensis Fruit Oil and their Effect on Propionibacterium acnes-Induced Inflammation in HaCaT Cells

Frontiers in Bioscience-Landmark (2023)

Pingping Lv Huirong Zhang Meiling Tai Biao Che Dan Yu

Propionibacterium acnes causes upregulation of inflammatory factors, such as cycloxygenase-2, prostaglandin E2, interleukin-1β, and tumor necrosis factor-alpha, increased levels of reactive oxygen species (ROS) and inward flow of calcium ions. This causes increased levels of the antimicrobial peptide LL-37 and inflammation of the skin, leading to redness, swelling, itching and other symptoms. Schisandra chinensis fruit oil (SCO) is rich in lignan active ingredients with various antioxidant and anti-inflammatory properties.In this study, SCO is obtained by supercritical CO2 fluid extraction. SCO's anti-inflammatory actions were investigated using P. acnes-induced inflammation HaCaT cells model. A method based on reversed-phase high-pressure liquid chromatography with a diode array detector was developed and validated for the simultaneous detection of five lignan components. Levels of inflammatory factors and LL-37 were measured by ELISA kit and western blot respectively. Ca2+ and ROS levels detected by flow cytometry.The experimental results show that the contents of schisanol A, schisanol B, schisanin A, schisanin B, and schisanin C were 33.89 ± 0.24, 14.89 ± 0.45, 8.92 ± 0.02, 29.14 ± 0.67, and 4.74 ± 0.09 mg/g, respectively. Studies have demonstrated that SCO can alleviate skin inflammation by inhibiting the COX-2/PGE2 and NF-κB signalling pathway. In addition, SCO can inhibit ROS production, significantly block inward Ca2+ flow, alleviate cell damage, and modulate the content of the antimicrobial peptide LL-37.In summary, our study elucidated the anti-inflammatory activity of SCO in a cell model and provided a scientific basis for its application as a raw material in skin care.

HaCaT

Croton oil

ABTS

10.31083/j.fbl2808177

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Pediatric and Adult Obesity Concern Female Health: A Mendelian Randomization Study

Research Square (Research Square) (2021)

Yi‐Shang Yan Zihao Qu Pingping Lv Hefeng Huang

Abstract Purpose: Adulthood and childhood obesity are associated with female reproductive endocrinology and gynecological cancers. However, the causality of such association is yet to be identified. Independent of inverse bias and confounding, mendelian randomization is like a random control trial where genetic groups are settled during meiosis, which could be effective to examine the causality. Methods : We carried out several Mendelian randomization trials based on combined genetic scores of 75 adult-associated and 15 child-associated BMI single nucleotide polymorphisms (SNPs), diseases databases of several gynecological cancers and reproductive diseases from UK Biobank with 194,153 participants, using traditional inverse-variance weighted (IVW) method, Weighted Median (WM) approach, MR-Egger regression and updated MR-PRESSO analysis. Results: Elevated adult-associated BMI (effect:1.003;95%CI:1.001,1.004) and child-associated BMI (effect:1.003;95%CI:1.001,1.004) genetic scores were related to higher risk of PCOS incidence by using traditional IVW method. Random IVW method showed a negative causal association between the child-associated BMI and subsequent endometriosis attack(effect:0.995;95%CI:0.991,0.999). Conclusions: Consistent with observational consequences, our findings suggested that childhood and adulthood obesity may play roles in the development of PCOS, and children obesity can elevate the possibility of PCOS but decrease the incidence of endometriosis in later life. More researches need to be conducted for further validation and potential mechanisms.

Mendelian Randomization

Causality

10.21203/rs.3.rs-390994/v1

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COX-2 inhibitor nimesulide protects rat heart against oxidative stress by improving endothelial function and enhancing NO production.

PubMed (2007)

Pingping Lv Ying Fan Wenliang Chen Yue‐Liang Shen Li Zhu

Since a cyclooxygenase 2 (COX-2) inhibitor can reduce infarct size and improve contractility in ischemic myocardium, the aim of the present study was to explore whether COX-2 inhibitor nimesulide could protect myocardial function against oxidative stress injury in rat hearts, and to investigate the underlying mechanisms. The isolated rat hearts perfused by Langendorff method were exposed to 140 mumol/L of H2O2, and the cardiac contractility was measured. Then, the responses of coronary arteries, precontracted with U-46619, to the endothelium-dependent vasodilator serotonin (5-HT) and the endothelium-independent vasodilator sodium nitroprusside (SNP) were evaluated. The results were as follows: (1) In hearts exposed to H2O2 for 20 min, the left ventricular developed pressure [LVDP, (54.8 +/- 4.0)%] and maximal rate of rise/fall of ventricular pressure [+/-dp/dt(max), (50.8 +/- 3.1)% and (46.2 +/- 2.9) %] were reduced compared with that in the control group (100%). After pretreatment with nimesulide (5 mumol/L) for 10 min before H2O2 perfusion, LVDP and +/-dp/dt(max) were enhanced to (79.9 +/- 2.8)%, (80.3 +/- 2.6)% and (81.4 +/- 2.6)%, respectively (P<0.01), and this was partially abolished by the nitric oxide synthase (NOS) inhibitor L-NAME [(60.2 +/- 2.1)%, (63.9 +/- 2.4)% and (63.1 +/- 2.9)%, respectively, P<0.01]. (2) The vasodilatation induced by 5-HT and SNP in H2O2-treated group was significantly less than that in the control group. Pretreatment with nimesulide for 10 min antagonized the decrease of endothelium-dependent vasodilatation in H2O2-treated group [(-22.2 +/- 4.2) % vs (-6.0 +/- 2.5) %, P<0.01], but had no effect on the decline of endothelium-independent vasodilatation [(-2.0 +/- 1.8)% vs (-7.0 +/- 3.5) %, P>0.05]. (3) Pretreatment with nimesulide for 10 min increased the NO production in H2O2-treated hearts [(2.63 +/- 0.40) vs (1.36 +/- 0.23) nmol/g protein, P<0.05], and this was inhibited by L-NAME. (4) Pretreatment with the selective COX-1 inhibitor piroxicam had no effect on LVDP and +/-dp/dt(max) in isolated hearts exposed to H2O2, but the left ventricular end diastolic pressure (LVEDP) was much higher than that in the group treated with H2O2 alone. Piroxicam did not influence the coronary resistance in H2O2-treated rat hearts. These data suggest that the COX-2 inhibitor nimesulide improves myocardial function in rat hearts suffering from oxidative stress, and this may be through an improvement in endothelium-dependent arterial relaxation and an enhancement of NO production in rat heart.

Nimesulide

Sodium nitroprusside

Contractility

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HID-1 is a Novel Player in the Regulation of Blood Glucose

Biophysical Journal (2014)

Wen Du Pingping Lv Dongwan Cheng Eli Song Tao Xu

10.1016/j.bpj.2013.11.238

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Sustained Endoplasmic Reticulum Stress as a Cofactor of Oxidative Stress in Decidual Cells from Patients with Early Pregnancy Loss

Endocrinology (2011)

Aixia Liu Weihua He Lijun Yin Pingping Lv Yu Zhang

Background: Oxidative stress (OS) is a common pathological background for different etiologies of early pregnancy loss (EPL). It has been suggested that elevated reactive oxygen species trigger endoplasmic reticulum (ER) stress by influencing ER function. However, it is unclear whether ER stress is associated with EPL. Objectives: The aim of the study was to determine whether and how ER stress occurs during the development of EPL. Approaches: Proteomic analysis was performed on decidua from women with EPL, and then ER stress markers, redox status, apoptotic features, and cell viability were analyzed in EPL decidual cells (DCs). Results: EPL decidua were characterized by decreased levels of glucose-regulated protein 78 (GPR78) and valosin-containing protein and burdened with ubiquitinated proteins. Evidence of ER stress-induced apoptosis in EPL DCs was demonstrated by extensive dilation of ER, morphological features of apoptosis, and activation of caspase-4 and caspase-12. Furthermore, H2O2 reduced the viabilities in both EPL and control DCs, whereas EPL DCs were more vulnerable to additional OS challenge than the controls as a result of failed induction of GRP78 expression. The cell survival percentages of DCs were dose-dependently reduced by H2O2 and could be reversed in the presence of vitamin E. This effect was partly mediated by reducing the amount of misfolded proteins rather than regulating GRP78 expression. Conclusions: The sum of these observations demonstrate for the first time that sustained ER stress occurs in EPL DCs and the potentially vicious relationship between ER stress and OS is likely to play an important role in the development of EPL.

Decidua

Decidual cells

10.1210/endo.152.1.9994

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A Novel F-SVM based on PSO for Gait Phase Recognition in Lower Limb Exoskeleton

2022 IEEE 17th Conference on Industrial Electronics and Applications (ICIEA) (2023)

Pingping Lv Chi Zhang Yi Feng Ting Yuan Shupei Li

To meet the requirements of lower limb exoskeleton robots for lightweight equipment and accurate gait phase detection under different speeds, the kinematics and dynamics information are investigated. In this paper, we propose a novel radius-margin-based supporting vector machine (SVM) model with particle swarm optimization algorithm (PSO) in feature space called PSO-F-SVM for gait phase detection. The proposed method considers both the maximization of margin and the minimization of radius and uses PSO to optimize the parameter in F-SVM, which can enhance the performance of the SVM classifier. Plantar pressure insoles with flexible and elastic sensors are designed for gait feature measurement. We conduct comparative experiments between the proposed method and other variants of SVM under four speeds. The experimental result indicates that the proposed method improves the accuracy by 2.68% compared with the standard SVM. Moreover, more than 98% accuracy can be obtained at four speeds, which demonstrates that the proposed method outperforms the other variants of SVM.

Maximization

Margin (machine learning)

Feature (linguistics)

Feature vector

10.1109/iciea58696.2023.10241537

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High Maternal Serum Estradiol Environment in the First Trimester Is Associated With the Increased Risk of Small-for-Gestational-Age Birth

The Journal of Clinical Endocrinology & Metabolism (2014)

Xiao Hu Chun Feng Xian-Hua Lin Zi-Xing Zhong Yimin Zhu

Context: There are increasing concerns that a disrupted endocrine environment may disturb the growth of the fetus. Assisted reproductive technology (ART) situates gamete/embryo in a supraphysiological estradiol (E2) environment and, thus, provides an ideal model to investigate this problem. Objective: Our objective was to investigate whether the maternal high-E2 environment in the first trimester increases the risks of low birth weight (LBW) and small-for-gestational-age (SGA) birth. Methods: In total, 8869 singletons born after fresh embryo transfer (ET) (n = 2610), frozen ET (n = 1039), and natural conception (NC) (n = 5220) and their mothers were included. Birth weight, LBW, SGA, and maternal serum E2 levels were investigated. Results: The mean serum E2 levels of women undergoing fresh ET at 4 and 8 weeks of gestation were significantly higher than those of the women undergoing frozen ET and the women with NC (P < .01). Serum E2 levels of women undergoing fresh ET at 4 and 8 weeks of gestation were positively correlated to those on the day of human chorionic gonadotropin (hCG) administration (r = 0.5 and r = 0.4, respectively; P < 0.01). The birth weight after fresh ET was significantly lower than that after frozen ET and NC (P < 0.01), with increased incidence of LBW and SGA (P < .05). Furthermore, in the fresh ET group, singletons of mothers with high E2 levels (≥10460 pmol/L on the day of hCG administration) had higher risks of LBW (P < .01) and SGA (P < .01) than those with low E2 levels, and maternal serum E2 level on the day of hCG administration negatively correlated with the birth weight (P < .01). Conclusions: The maternal high-E2 environment in the first trimester is correlated with increased risks of LBW and SGA. Evaluation of serum E2 before ET should be adopted to reduce the possibility of high E2 exposure to gamete/embryo.

Assisted Reproductive Technology

10.1210/jc.2013-3362

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Citations (106)

Requirements for confirmation of PCR-RFLP results of polymorphisms

International Journal of Reproductive BioMedicine (IJRM) (2019)

Chun Feng Pingping Lv Min Jin Jin‐Ming Shen Tianhe Huang

10.18502/ijrm.v17i3.4521

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COX‐2 inhibitor protects rat hearts against oxidative stress through endothelial function improvement and NO enhancement

The FASEB Journal (2007)

Pingping Lv Yingying Chen Li Zhu Linlin Wang Yue‐Liang Shen

The study was to explore whether cyclooxygenase 2 (COX-2) inhibitor could protect the myocardial function against oxidative stress injury in rat hearts and to investigate its mechanisms. Isolated rat hearts perfused by the Langendorff method were exposed to H2O2 (140¦ÌM/L), after that coronaries were precontracted with U-46619, and then the response to the endothelium-dependent vasodilator 5-HT and endothelium-independent SNP were evaluated. Exposure hearts to H2O2for 20 min, systolic function were reduced. Pretreatment with COX-2 inhibitor nimesulide (5¦ÌM/L) enhanced the LVDP and ¡ÀdP/dtmax, which could be abolished by NOS inhibitor L-NAME. The vasodilatation produced by 5-HT and SNP in H2O2group was significantly less than in that of control group. Pretreatment with nimesulide antagonized the decrease in endothelium-dependent vasodilatation, but had no effect on the decline in endothelium-independent vasodilatation. The concentration of 6-keto PGF1¦Á was no different among all groups. These data suggest that nimesulide could improve myocardial function in rat hearts suffered from oxidative stress. The mechanisms may be through endothelial function improvement and NO enhancement, but not via inhibiting COX-2 activity. (Project Supported by the National Natural Science Foundation of China (NO. 30400094) ).

Nimesulide

Endothelial Dysfunction

10.1096/fasebj.21.6.a1254

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Effect of lncRNA MALAT1 on the Granulosa Cell Proliferation and Pregnancy Outcome in Patients With PCOS

Frontiers in Endocrinology (2022)

Mixue Tu Yiqing Wu Feixia Wang Yun Huang Yu-Li Qian

Follicle arrest is one of the main characteristics of polycystic ovary syndrome (PCOS), the most common endocrinological disorder in reproductive-aged women. Increasing evidence proves that high anti-Mullerian hormone (AMH) levels may play an important role in follicular development. Long noncoding RNA (lncRNA) with a length of more than 200 nt is widely involved in the directional differentiation, growth, and development of cells, whereas whether lncRNA is involved in AMH's role in follicular development is unknown. In this study, we analyzed lncRNA expression in ovarian granulosa cells (GCs) collected from women with and without PCOS via high-throughput sequencing. The results showed that a total of 79 noncoding transcripts were differently expressed in GCs of PCOS patients, including upregulated lncRNA MALAT1. The upregulation of MALAT1 was further confirmed by RT-qPCR in GCs from a larger cohort of PCOS patients. Furthermore, knockdown MALAT1 can promote the proliferation of KGN cell in vitro. These data suggested a role for MALAT1 in the development of PCOS. Meanwhile, MALAT1 and phosphorylated SMAD 1/5 (Ser463/465) protein were upregulated in KGN cells after exogenous AMH stimulation, which identified AMH perhaps as a regulator for the expression of MALAT1. We also found that MALAT1 can predict clinical pregnancy outcome to a certain extent by ROC curve analysis (area: 0.771, p = 0.007, 95% CI: 0.617-0.925, sensitivity: 57.1%, specificity: 91.7%). Thus, our findings revealed a role of lncRNA MALAT1 in inhibiting granulosa cell proliferation and may be correlated with pregnancy outcome in PCOS.

Anti-Müllerian hormone

MALAT1

Ovarian Reserve

10.3389/fendo.2022.825431

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Citations (20)