【Objective】To investigate the feasibility and safety of Laparoscopic modified Sugiura procedure by dissecting secondary braches of splenic pedicle in patients suffering from portal hypertension.【Methods】15 patients suffering from portal hypertension underwent Laparoscopic modified Sugiura procedure by dissecting secondary braches of splenic pedicle.【Results】Laparoscopic modified Sugiura procedure by dissecting secondary braches of splenic pedicle were performed safely with no conversion to open surgery and no severe postoperative complication.Mean surgical time was 256.5 minutes.Mean blood loss 193 mL and mean postoperative hospital stay was 10.2 days.【Conclusions】Laparoscopic modified Sugiura procedure by dissecting secondary braches of splenic pedicle in patients suffering from portal hypertension is safe and feasible.
// Sun Yong 1 , Yu Yabin 1 , Zhou Bing 1 , Zhu Chuanrong 1 , Gu Dianhua 1 , Zhang Jianhuai 1 , Yuan Weidong 1 , Wang Shuming 1 and Liu Ling 1 1 Department of Hepatobiliary and Pancreatic Surgery, Huai'an First People's Hospital, Nanjing Medical University, Huai'an, Jiangsu 223300, People's Republic of China Correspondence to: Liu Ling, email: liulingdoc_2002@aliyun.com Keywords: PDAC, DGCR5, miR-320a, lncRNA Received: March 13, 2017 Accepted: April 17, 2017 Published: June 06, 2017 ABSTRACT Pancreatic ductal adenocarcinoma (PDAC) is one of the most aggressive and lethal malignancies. Long non-coding microRNAs (lncRNAs) are a newly discovered type of regulatory molecule with both diagnostic and prognostic value, but the role of lncRNA in PDAC has not been well investigated until now. Here, we present evidence that shows that the lncRNA DGCR5 is significantly reduced in PDAC tissues as well as in PDAC cell lines and that the downregulation of DGCR5 predicts poor prognosis. Ectopic expression of DGCR5 inhibits the proliferation and migration, and promotes 5-FU resistances of PDAC cells. Further experiments demonstrated that DGCR5 and miR-320a regulate each other in a reciprocal manner and that DGCR5 reverses the inhibition of PDCD4 by miR-320a, which is involved in the regulation of the PDAC cell phenotype and response to 5-FU. Our findings provide novel information about the functions of lncRNAs in PDAC, some of which might be beneficial to the precise diagnosis, prognosis and individualized therapy of patients with PDAC in the future.
Hepatocellular carcinoma (HCC) is one of the most common malignancies in the world, and the prognosis for the HCC remains very poor. Although dys-regulation of CIZ1 (Cip1 interacting zinc finger protein 1) has been observed in various cancer types, its expression and functions in HCC remain unknown. In this study, the mRNA level of CIZ1 in the HCC tissues were examined using real-time polymerase chain reaction, and the effects of CIZ1 on the growth, migration, and metastasis of HCC cells were examined by crystal violet assay, Boyden chamber assay, and in vivo image system, respectively. In addition, the molecular mechanisms were investigated by luciferase assay. Upregulation of CIZ1 in the clinical HCC samples was observed. Forced expression of CIZ1 promoted the growth and migration of HCC cells, while knocking down the expression of CIZ1 inhibited the growth, migration, and metastasis of HCC cells. Molecular mechanism studies revealed that CIZ1 activated YAP/TAZ signaling in HCC cells. Taken together, our study demonstrated the oncogenic roles of CIZ1 in HCC cells and CIZ1 might be a promising therapeutic target for HCC.
Circulating (serous or plasmic) long non-coding RNA (lncRNA) as biomarkers for predicting the diagnosis or prognosis of human disease have been well documented. Due to the sensibility or specificity limitation of Alpha Fetoprotein (AFP), a cluster lncRNAs were revealed as fingerprints for hepatocellular carcinoma (HCC). In this study, we enrolled all the reported circulating lncRNAs in HCC as candidate targets and examined in an independent cohort.The candidate lncRNAs were determined by qRT-PCR divided into training and validation sets. The risk score analysis was employed to evaluate the potential diagnosis ability of the lncRNAs independently or combining with AFP value. The receiver operating characteristic curve (ROC) was applied for presentation of sensibility or specificity.Among the ten candidate circulating lncRNA, LINC00152, RP11-160H22.5, XLOC014172 and LOC149086 were screened with significant difference in training set. Further investigation in validation set indicated LINC00152, RP11-160H22.5 and XLOC014172 might be the fingerprints for HCC comparing with chronic hepatitis (CH) patients or healthy controls. The risk score analysis revealed the combination of three lncRNAs with AFP could distinguish the HCC from either CH or healthy control with the area under curve value (AUC) of 0.986 and 0.985, respectively.The three lncRNAs may act as novel biomarkers for acting as fingerprint in HCC combining with AFP.
CD97 as a member of the EGF-TM7 family with adhesive properties plays an important role in tumor aggressiveness by binding its cellular ligand CD55, which is a complement regulatory protein expressed by cells to protect them from bystander complement attack. Previous studies have shown that CD97 and CD55 both play important roles in tumor dedifferentiation, migration, invasiveness, and metastasis. The aim of this study was to investigate CD97 and CD55 expression in primary gallbladder carcinoma (GBC) and their prognostic significance.Immunohistochemistry was used to investigate the expression of CD97 and CD55 proteins in 138 patients with GBC.CD97 and CD55 were absent or only weakly expressed in the normal epithelium of the gallbladder but in 69.6% (96/138) and 65.2% (90/138) of GBC, respectively, remarkably at the invasive front of the tumors. In addition, CD97 and CD55 expressions were both significantly associated with high histologic grade (both P = 0.009), advanced pathologic T stage (P = 0.01 and 0.009, resp.) and clinical stage (both P = 0.009), and positive venous/lymphatic invasion (both P = 0.009). Multivariate analyses showed that CD97 (hazard ratio, 3.236; P = 0.02) and CD55 (hazard ratio, 3.209; P = 0.02) expressions and clinical stage (hazard ratio, 3.918; P = 0.01) were independent risk factor for overall survival.Our results provide convincing evidence for the first time that the expressions of CD97 and CD55 are both upregulated in human GBC. The expression levels of CD97 and CD55 in GBC were associated with the severity of the tumor. Furthermore, CD97 and CD55 expressions were independent poor prognostic factors for overall survival in patients with GBC.
Objective To investigate the factors that related to the metastasis of portal lymph node in the patients with primary liver cancer,the patterns of lymphatic invasion and the influence on prognostic of the patients received lymphadenectomy.Methods 113 patients with primary liver cancer underwent the resection of portal lymph node before the conventional hepatectomy.Incidence rate,location,coincidence of pre-and postoperative diagnosis and the influence on prognosis of node metastasis were analyzed.Results The metastasis of portal lymph node existed in 18 cases(15.93%)among 113 cases of primary liver cancer,including 10 cases of hepatocelluar carcinoma,7 cases of cholangiocellular carcinoma,and 1 cases of mixed hepatoma.Lymph nodes near periportal,bile duct,and common hepatic artery were all removed.The median number of resected nodes was 3.8 ± 1.6.The median survival time of the patients with the metastasis of portal lymph node was 13 monthes;while the median survival time of the patients without the metastasis was 25 monthes.Their 2-year survival rate was 17.12% and 49.83% for patients with and without portal node metastasis,and there is significant difference between them.Conclusion Lymph node status is one of the most important prognostic factors for the patients of carcinoma.The resection of portal lymph node is safe,and should be routinely applied in patientswith cholangiocellular carcinoma,especially in the patients without chronic disease.
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