In order to make the thermo-hygrometer calibrator more intellectualized, an automatic indication recognition algorithm of dual-pointer meter is proposed. Firstly, Hough circle transform algorithm is used to segment the image and intercept the dial; secondly, the color feature of the image is used to detect the datum point and correct the image; then, the image of pointer is extracted by the improved Zhang thinning algorithm and progressive probabilistic Hough transform algorithm; finally, the algorithm is evaluated by the images of meter captured in an experimental platform of the thermo-hygrometer calibrator. The results show that the algorithm proposed in this paper can recognize the indication of the meter with high accuracy and robustness.
The disease symptom, the pathogenesis and control measures of stem nematode disease in sweet potato were introduced. The research progress at home and abroad of resistance breeding for stem nematode disease in sweet potato was summarized from the traidtional and molecular of disease-resistant breeding. It was pointed out that resistant varieties was the most economic and effective method of controlling the disease.
Griffithsin (GRFT) is a highly effective, broad-spectrum, safe, and stable viral inhibitor used to suppress a variety of viruses. However, little information is available on whether GRFT can prevent plant viral diseases. In this study, we constructed a GRFT overexpression vector containing the sweetpotato storage cell signal peptide and generated exogenous GRFT overexpression lines through genetic transformation. The transgenic plants showed notable resistance to sweetpotato virus disease in the virus nursery. To verify the antiplant virus function of GRFT, transient expression in tobacco leaves showed that GRFT inhibited the sweetpotato leaf curl virus (SPLCV). The replication of SPLCV was entirely inhibited when the concentration of GRFT reached a certain level. The results of pulldown and BIFC assays showed that GRFT did not interact with the six components of SPLCV. In addition, the mutated GRFT D/A without the binding ability of carbohydrate and anticoronavirus function, in which three aspartate residues at carbohydrate binding sites were all mutated to alanine, also inhibited SPLCV. Quantitative reverse-transcription PCR analyses showed that the tobacco antiviral-related genes HIN1 , ICS1 , WRKY40 , and PR10 were overexpressed after GRFT/GRFT D/A injection. Furthermore, HIN1 , ICS1 , and PR10 were more highly expressed in the leaves injected with GRFT D/A . The results suggest that sweetpotato is able to express GRFT exogenously as a bioreactor. Moreover, exogenous GRFT expression inhibits plant viruses by promoting the expression of plant antiviral genes.
Nine sweet potato varieties with different flesh colors were cultivated under uniform environmental conditions with potassium (K) fertilizer treatments at levels of 0, 22.5, and 45 kg/ha. The structural and functional properties of the starches were subsequently analyzed. The soluble sugar content in the dry root tuber increased, with higher K levels in most varieties. Amylose content decreased in Sushu16 but increased in Ningzishu1, with no significant differences observed in other varieties across different K levels. Elevated K levels had no effect on starch protein content, crystalline type, or gelatinization enthalpy. The impact of K fertilizer on starch thermal and pasting properties varied among the varieties. PLSR and PLS-DA analyses revealed that genotype background was the primary factor influencing starch properties. This research will provide a reference for the improvement of sweet potato production quality and efficiency and a scientific basis for the cultivation and utilization of sweet potato root tubers.
Sweetpotato [Ipomoea batatas (L.) Lam.], a food crop with both nutritional and medicinal uses, plays essential roles in food security and health-promoting. Chlorogenic acid (CGA), a polyphenol displaying several bioactivities, is distributed in all edible parts of sweetpotato. However, little is known about the specific metabolism of CGA in sweetpotato. In this study, IbPAL1, which encodes an endoplasmic reticulum-localized phenylalanine ammonia lyase (PAL), was isolated and characterized in sweetpotato. CGA accumulation was positively associated with the expression pattern of IbPAL1 in a tissue-specific manner, as further demonstrated by overexpression of IbPAL1. Overexpression of IbPAL1 promoted CGA accumulation and biosynthetic pathway genes expression in leaves, stimulated secondary xylem cell expansion in stems, and inhibited storage root formation. Our results support a potential role for IbPAL1 in sweetpotato CGA biosynthesis and establish a theoretical foundation for detailed mechanism research and nutrient improvement in sweetpotato breeding programs.
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