Objective To investigate the mechanism of colorectal carcinogenesis furtherly.Methods Sixty specimens of colorectal cancers(colorectal cancer group) and 20 adenomas(adenoma group)were selected,another 44 normal mucosa(normal group)were taken from excisional margin of cancerous tissues,Immunohistochemical method was used to detect the expression of p53 up-regulated modulator of apoptosis(PUMA),cyclooxygenase-2(COX-2) and Caspase-3 in three groups,the method of correlation analysis of contingency table in numeration data was used to analyze the correlations among three indexs and their correlation with clinicopathologic features of colorectal cancer.Results The positive rates of PUMA,COX-2 and Caspase-3 in colorectal cancer group were 31.7%,78.3% and 66.7% respectively,which in adenoma group were 30.0%,75.0% and 70.0% respectively,and in normal group were 63.6%,84.1% and95.5% respectively.The positive rates of PUMA and Caspase-3 in adenoma group and colorectal cancer group were lower than that in normal group,all P0.01;The positive rates of COX-2 were also lower than that in normal group,P0.05.There was no significant difference among the positive rates of three indexs in colorectal cancer group and adenoma group.The expression of PUMA was correlated with tumor diameter of colorectal cancer,but was not with the other clinicopathologic features;the expression of Caspase-3 and COX-2 was not correlated with clinicopathologic parameters of colorectal cancer.A positive correlation was noted between the expression of PUMA and Caspase-3 in colorectal cancer.Conclusion The down regulation of PUMA and Caspase-3 expression in colorectal cancer may play certain roles in the early stage of colorectal carcinogenesis.
Objective: To determine the role of microsatellite alterations in carcinogenesis of colorectal carcinoma (CRC). Methods: Alterations of 10 microsatellite loci from 5 different chromosomes were detected in 92 colorectal cancers and their paired normal mucosa by PCR, denatured polyacrylamide gel electrophoresis and silver staining. Associations of microsatellite alterations with clinopathologic parameters were statistically clarifield. Results: Alterations of microsatellite were classified into microsatellite instability type I, type II and loss of heterozygosity (LOH). The carcinoma with ≧30% loci microsatellite alterations was defined as replication error(RER) positive tumors. Of 92 cases, 14 were RER+. Microsatellite alterations of P53(1) and D18S363 loci (64.29%) was most commonly identified in the RER+ tumors. RER+ were more commonly seen in poorly differentiated carcinomas and tended to occur in mucoid carcinomas. The type of microsatellite alterations varied in different histological types of CRC. Conclusions: Microsatellite alteration is a common molecular event in CRC. Different microsatellite loci showed various biologic significance. P53(1) and D18S363 should be essentially detected loci that can show the RER status of tumors.
Objective: To observe the expression of PUMA and caspase-3 in colorectal cancer and adenoma,and study the significance of PUMA and caspase-3 in carcinogenesis and development of colorectal carcinoma.Methods: Immunohistochemical method was used to detect the expression of PUMA and caspase-3 in 44 cases of normal colorectal mucosa,20 cases of colorectal adenoma and 60 cases of colorecral carcinoma,and analyzed their expressions and their correlation with clinical pathological parameters of colorectal carcinoma.Results: The expression of PUMA in normal colorectal mucosa tissues was higher than that in colorectal adenoma and colorectal cancer(P0.05),the positive rate of PUMA in normal colorectal mucosa tissues,colorectal adenoma tissues and colorectal cancers were 63.6%,30.0% and 31.7%,respectively;The expression of PUMA in colorectal cancinoma correlated with the tumor size(P0.05),but did not correlate with the other clinical pathological parameters(P0.05).The expression of caspase-3 in normal colorectal mucosa tissues was higher than that in colorectal adenoma and colorectal cancer(P0.05),the positive rate of caspase-3 in normal colorectal mucosa tissues,colorectal adenoma tissues and colorectal cancers were 95.5%,70.0% and 66.7%,respectively;The expression of caspase-3 in colorectal cancer did not correlate with clinical pathological parameters(P0.05).The expression of PUMA correlated with the expression of caspase-3 in colorectal carcinoma(r=0.329,P0.05).Conclusion: The down regulation of PUMA and caspase-3 may take part in the carcinogenesis of colorectal cancer.
Objective: To study the expression of Cyclin D1 and heat shock protein 70(HSP70) in hepatocellular carci-nomas. Methods:Tissue array was made from 145 patients with HCC, 78 patients with post-hepatitic cirrhosis, and 16 nor-mal controls. Immunohistochemical staining was performed on the slides. Staining index ( SI ) was used to assess the expres-sion of Cyclin D1 and HSP70. Results:The expression of Cyclin D1 and HSP70 were significantly higher in carcinoma (3.43 ±2.11 and 3.18 ± 2.13 ) than that in cirrhosis (1.51 ± 2.19 and 1.04 ± 1.85) and in normal control (0 and 0.75 ± 1.39) (P 0.01, respectively). The expression of Cyclin D1 was significantly related to the expression of HSP70 ( r = 0.219 , P 0.01). Conclusion:The excessive expressions of Cyclin D1 and HSP70 may play an important role in the course of carcinogenesis.
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