A multi-functional luminol-reduced Pt@Au hybrid flower-like nanocomposite (luminol-Pt@AuNF) which not only acts as an efficient signal probe but also constitutes a pseudobienzyme amplifying system with choline oxidase (ChOx) was firstly synthesized and applied to the construction of a solid-state luminol electrochemiluminescence (ECL) immunosensor for cardiac troponin I (cTnI) detection.

Luminol

Choline oxidase

Electrochemiluminescence

Choline

10.1039/c4cc05269b

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Citations (45)

Electrochemiluminescence Immunosensor Based on Platinum Nanoparticles for the Determination of Apolipoprotein A1

Journal of Electrochemistry (2016)

Ni Liao Zhuo Ying Ruo Yuan

Electrochemiluminescence

Platinum nanoparticles

10.13208/j.electrochem.150914

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Monitoring response to neoadjuvant therapy for breast cancer in all treatment phases using an ultrasound deep learning model

Research Square (Research Square) (2023)

Jingwen Zhang Jingwen Deng Jin Huang Liye Mei Ni Liao

Abstract Purpose: The present study investigated whether deep learning models (DLMs) could replace traditional ultrasound measurement models for predicting pathological responses to neoadjuvant chemotherapy (NAC) for breast cancer. Methods: Data from 57 patients (443 ultrasound images) who underwent NAC followed by surgery were analyzed. A DLM was developed for accurate breast tumor ultrasound image segmentation. The predictive abilities of the DLM, manual segmentation model (MSM), and two traditional measurement models (longest axis model [LAM] and dual-axis model [DAM]) for pathological complete response (pCR) were compared using tumor size ratios and receiver operating characteristic curves. Results: The average intersection over the union value of the DLM was 0.8087. MSM showed the best performance with an area under the curve (AUC) of 0.840; DLM performance was slightly weaker with an AUC of 0.756. The AUCs of the two traditional models were 0.778 for LAM and 0.796 for DAM. There was no significant difference in AUC values of the predictive ability of the four models. Moreover, no significant difference in AUC values of ultrasound prediction was noted between each NAC cycle (p<0.05). Conclusion: Patients in the pCR group had a significantly better response than those in the non-pCR group, and ultrasonography was predictive of pCR in the early stages of NAC. DLMs can replace traditional measurements for predicting pCR.

Area under curve

10.21203/rs.3.rs-3015100/v1

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Metal-organic Frameworks (MOF)-based Novel Electrochemiluminescence Biosensing Platform for Quantification of H₂O₂ Releasing from Tumor Cells

Acta Chimica Sinica (2021)

Ni Liao Xia Zhong Wenbin Liang Ruo Yuan Ying Zhuo

a 梁文斌 a 袁若 a 卓颖 * ,a ( a 西南大学化学化工学院发光分析与分子传感教育部重点实验室重庆 400715) ( b 攀枝花学院生物与化学工程学院攀枝花 617000) 摘要本工作借助金属-有机框架(MOF)结构, 通过配位作用实现对电化学发光(ECL)分子的空间有序组装, 获得了高性能 ECL 新材料, 发展了孔道限域效应和微纳空间 ECL 分子有序组装的协同增强 ECL 新策略.具体而言, 以非共平面 ECL 分子 1,1,2,2-四(4-羧基苯)乙烯(H4TCPE)为配体采用一步水热法合成了具有优异 ECL 性能的新型 MOF 材料 (Zr-TCPE).Zr-TCPE 通过将发光配体 H4TCPE 固定在 MOF 多孔材料上, 一方面其多孔结构可以促进限域空间内物质和电子的传递, 加快电化学反应的效率; 同时, MOF 结构的配位键合作用限制了发光基团的自由转动与振动以及分子间的紧密堆积, 从而有效减少了非辐射弛豫和内滤效应.和 H4TCPE 的微纳晶体相比, Zr-TCPE 的 ECL 强度提高了 3.46 倍, ECL 效率提高了 17.44 倍. 采用 Zr-TCPE 构建了 ECL 生物传感平台, 成功实现了在药物刺激下肿瘤细胞释放过氧化氢(H2O2)的原位监测, 对临床检测肿瘤细胞具有指导意义.

Electrochemiluminescence

10.6023/a21050223

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A supersandwich electrochemiluminescence immunosensor based on mimic-intramolecular interaction for sensitive detection of proteins

The Analyst (2014)

Yingning He Yaqin Chai Ruo Yuan Haijun Wang Lijuan Bai

An electrochemiluminescence (ECL) immunoassay protocol was developed based on mimic-intramolecular interaction for sensitive detection of prostate specific antigen (PSA). It was constructed by integrating the ECL luminophore (tris(4,4′-dicarboxylicacid-2,2′-bipyridyl)-ruthenium(II)dichloride (Ru(dcbpy)32+)) and coreactant (histidine) into the supersandwich DNA structure. This strategy was more effective in amplifying the ECL signal by shortening the electronic transmission distance, improving the ECL luminous stability and enhancing the ECL luminous efficiency. The ECL matrices denoted as MWCNTs@PDA–AuNPs were fabricated through spontaneous oxidative polymerization of dopamine (DA) on multiwalled carbon nanotubes (MWCNTs) and reducing HAuCl4 to produce gold nanoparticles (AuNPs) by DA simultaneously. Then, the prepared matrices were applied to bind capture antibodies. Moreover, supersandwich Ab2 bioconjugate was designed using a PAMAM dendrimer to immobilize the detection antibody and supersandwich DNA structure. The PAMAM dendrimer, with a plurality of secondary and tertiary amine groups, not only facilitated high-density immobilization of the detection antibody and supersandwich DNA structure, but also greatly amplified the ECL signal of Ru(dcbpy)32+. The supersandwich DNA structure contained multiple Ru(dcbpy)32+ and histidine, further amplifying the ECL signal. The proposed supersandwich immunosensor showed high sensitivity with a detection limit of 4.2 fg mL−1 and a wide linear range of 0.01 pg mL−1–40.00 ng mL−1. With the excellent stability, satisfying precision and reproducibility, the proposed immunosensor indicates promising practicability for clinical diagnosis.

Electrochemiluminescence

Luminophore

Bioconjugation

10.1039/c4an01002g

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Citations (15)

Hollow platinum decorated Fe3O4 nanoparticles as peroxidase mimetic couple with glucose oxidase for pseudobienzyme electrochemical immunosensor

Sensors and Actuators B Chemical (2013)

Zhehan Yang Yaqin Chai Ruo Yuan Ying Zhuo Ya Li

Glucose oxidase

Bovine serum albumin

Biocompatibility

Platinum nanoparticles

10.1016/j.snb.2013.11.010

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Citations (46)

Expression and significance of nitric oxide synthase in mandibular distraction osteogenesis of canine

Nuo Zhou Ni Liao Shanliang Wei Feixin Liang

Objective To investigate the expression and significance of nitric oxide synthase(NOS)during mandibular distraction osteogenesis and bone trauma healing, to futher study the mechanism of distraction osteogenesis. Methods Twenty-eight adult dogs were randomly divided into DO group(12 dogs), acutely lengthening group(12 dogs) and control group(4 dogs). Immunohistochemical examination were carried out to test the expression of NOS during the sixth day in distraction period, the second and eighth week of consolidation. Results In DO group infiltration of inflammatory cell was found in the distraction gap, more red blood cells(RBC) leak out around vascellum and matrix in the sixth day in distraction period. The expression of local iNOS(inducible NOS) and eNOS(endothelinal NOS) in DO gruoup and acutely lengthening group was higher than that in the control group(P0.05), the expression of local iNOS and eNOS in acutely lengthening group was lower than that in DO group(P0.05)in the early of distraction period and the consolidation. The expression of local iNOS and eNOS was no statistic difference between three groups(P0.05) in the eighth week of consolidation. Conclusion NOS as a sensitive index of tissue trauma are highly expressed, and RBC was found leaking out in the early of distraction, indicating that micro-trauma to some extent may occurr during DO procedure, the micro-trauma may be one of the significant factors which increasing regeneration of bone during DO.

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DNA Structure Transition-Induced Affinity Switch for Biosensing Based on the Strong Electrochemiluminescence Platform from Organic Microcrystals

Analytical Chemistry (2020)

Ni Liao Jia‐Li Liu Yaqin Chai Ruo Yuan Ying Zhuo

Here we described an effective recognition strategy using the target-triggered DNA structure transition as an affinity switch for nucleic acid detection based on the strong electrochemiluminescence (ECL) platform of 9,10-diphenylanthracene (DPA) doped perylene (Pe) microcrystals (DPA@Pe MCs). Specifically, the target-triggered rolling-circle amplification (RCA) could generate a long, single-stranded DNA with repeated G-quadruplex units, which would hinder the access of quenching probes due to the steric hindrance effects offered by the DNA structure transition. Using this effective recognition strategy, an ECL biosensor with ultrasensitive and accurate characteristics was proposed to detect microRNA-21, which showed an excellent linear response from 10 aM to 1 pM with the detection limit down to 4.14 aM. The DNA structure transition-induced affinity switch strategy offered a potential applications in clinical diagnosis analysis.

Electrochemiluminescence

Rolling circle replication

10.1021/acs.analchem.9b05433

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Citations (21)

統計解析に基づく指静脈の個性に関する研究【Powered by NICT】

IEEE Conference Proceedings (2016)

Yapeng Ye Zheng He Ni Liao Liu Shilei Wenxin Li

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