Guangfu Yu

Shandong First Medical University

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Yu AiLian

China University of Geosciences

Dongming Zhuang

Tianjin University of Finance and Economics

Weifeng Shi

Nestlé (Germany)

Zhao Ying-hui

Northeast Agricultural University

Chao‐Dong Zhu

Chinese Academy of Sciences

Aishe Dun

Shandong First Medical University

Yanzhou Zhang

Harvard Stem Cell Institute

Huihui Wu

Jinan Central Hospital

Yuanyi Xu

Ningxia Medical University

Yunning Huang

The Fourth People's Hospital of Ningxia Hui Autonomous Region

Cooperative Institutions

Shandong First Medical University

Taishan Medical University

Chinese Academy of Sciences

Shanghai Jiao Tong University

Ningxia Medical University

Sun Yat-sen University

Shandong University

Capital Medical University

Chinese Academy of Agricultural Sciences

National Institute for Communicable Disease Control and Prevention

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Dextran sulfate inhibits proliferation and metastasis of human gastric cancer cells via miR-34c-5p

Heliyon (2024)

Yuan Zhao Qian Ma Wenwei Gao Zhaojun Li Guangfu Yu

Gastric cancer (GC) is a malignant tumor with a high global mortality rate that is currently difficult to treat. Dextran sulfate (DS), a safe anti-tumor agent, can effectively inhibit the malignant biological behavior of gastric cancer; however, its mechanism of action is not fully understood. Therefore, this study aimed at elucidate the potential mechanisms of action.

10.1016/j.heliyon.2024.e34859

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Citations (1)

[Differences in expression of Notch1, E-cadherin, vascular endothelial growth factor A and C, and chemokine receptor 4 gene in breast suspension mammosphere cells and normal adherent cells].

PubMed (2013)

Zhaohui Wang Chuanwei Li Yu AiLian Guangfu Yu Yinghui Zhao

Notch1是参与调控细胞分化的重要分子,其表达异常与许多肿瘤的转移密切相关.在乳腺癌发展过程中,Notch1分子可能通过增强乳腺癌干细胞迁徙活性促进其转移,如果证实这一机制,对于研制靶向于乳腺癌干细胞Notch1分子的药物,阻断其转移有重要意义。

VE-cadherin

10.3760/cma.j.issn.0529-5807.2013.05.011

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Targeting ADAR1 suppresses progression and peritoneal metastasis of gastric cancer through Wnt / β-catenin pathway

Journal of Cancer (2021)

Zhiyong Li Yunning Huang Yuanyi Xu Xiaofei Wang Honghong Wang

Objective: Peritoneal metastasis frequently occurs in advanced gastric cancer, which is typically not eligible for radical surgery. Here, this study observed the function and regulatory mechanism of ADAR1 in peritoneal metastasis of gastric cancer. Methods: ADAR1, CALR and β-catenin proteins were detected in normal mucosa, primary gastric cancer, metastatic lymph node and metastatic omentum tissues by immunohistochemistry, western blot, and immunofluorescence. After silencing ADAR1 by siADAR1, the effect and mechanism of ADAR1 on gastric cancer metastasis were observed in nude mouse models of gastric cancer with peritoneal metastasis as well as HGC-27 and AGS gastric cancer cells. Result: Our results showed that ADAR1 was significantly up-regulated in gastric cancer, metastatic lymph node and metastatic omentum tissues. Its up-regulation was significantly correlated to lymph node metastasis and peritoneal metastasis. Silencing ADAR1 significantly reduced the volume of peritoneal metastatic tumors and weakened oncogene CALR expression, Wnt / β-catenin pathway and epithelial-mesenchymal transition (EMT) process in vivo. Furthermore, ADAR1 knockdown distinctly suppressed cell viability, colony formation and migration of HGC-27 and AGS cells and ameliorated the effects of Wnt pathway activator on tumor progression. The similar findings were investigated when treated with ADAR1 inhibitor 8-Azaadenosine. Conclusion: Collectively, this study identified a novel oncogenic function of ADAR1 in peritoneal metastasis of gastric cancer via Wnt / β-catenin pathway. Hence, ADAR1 could be a novel marker and therapeutic target against gastric cancer metastasis.

10.7150/jca.61031

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Citations (16)

Deoxynivalenol impairs proliferation and induces apoptosis in primary murine osteoblasts

Toxicological & Environmental Chemistry Reviews (2018)

Huihui Wu Xiao Sun Zhaoqiang Zhang Dongming Zhuang Yu Wang

The effects of deoxynivalenol in different dose including 100, 200, 500, 1000, and 1500 µg/L on primary cranial osteoblasts from fetal mice were investigated. Fluorescence staining, flow cytometric measurement, 3-(4,5-dimethylthiazol 2-y1)-3,5-di-phenyltetrazolium bromide assay, quantitative PCR, and Western blot were used for the test. Mineralization and proliferation of osteoblasts decreased upon 100 µg/L and higher deoxynivalenol treatment and apoptosis of osteoblasts was increased upon 500, 1000, and 1500 µg/L deoxynivalenol treatment. Karyopyknosis, membrane breakage, and a decreased number of calcium nodes were also observed upon 500 µg/L deoxynivalenol treatment. The mRNA and protein levels of B-cell lymphoma-2-associated X protein were upregulated, B-cell lymphoma-2 protein downregulated with increasing concentrations of deoxynivalenol treatment and their ratio increased. Deoxynivalenol induces apoptosis of osteoblasts, suggesting a mechanism by which deoxynivalenol can affect murine skeletal development.

Cell counting

10.1080/02772248.2018.1458851

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Selection pressure on Haemagglutinin genes of H9N2 influenza viruses from different hosts

Virologica Sinica (2009)

Weifeng Shi Aishe Dun Zhong Zhang Yanzhou Zhang Guangfu Yu

Positive selection

Negative selection

10.1007/s12250-009-2988-5

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Identification and phylogenetic analysis of Nairobi sheep disease virus from Haemaphysalis longicornis ticks in Shandong Province, China

Ticks and Tick-borne Diseases (2024)

Yunxiao Wang Ruiling Zhang Xiurong Wang Xudong Zhang Zhong Zhang

Nairobi Sheep Disease (NSD) is a typical tick-borne syndrome characterized by severe hemorrhagic gastroenteritis, spontaneous abortion, and a high case fatality rate in small ruminants. The pathogenic agent, Nairobi sheep disease virus (NSDV), has also been associated with human infections, indicating its possible zoonotic potential. Prior to this study, NSDV has been detected from ticks collected in Jilin, Hubei, and Liaoning provinces in China. In the present study, a total of 343 ticks (Haemaphysalis longicornis) were collected in Shandong province, China in 2020, and pooled into 16 libraries. Analysis of the meta-transcriptomic sequencing data identified NSDV strains SDWL07, SDWL08, and SDWL16 from three pools. The SDWL07 and SDWL16 strains were detected from unfed ticks, while SDWL08 was detected from cattle-feeding ticks. Phylogenetic analyses showed higher sequence identities between the three strains and other Chinese NSDV strains than those from India and Kenya. Phylogenetic analyses also revealed that they clustered together and fell within the China lineage, suggesting no potential genetic reassortment among them. In summary, this is the first report of the identification of NSDV in Shandong province, highlighting the continually expanding endemic regions of this pathogen. Surveillance of NSDV should be intensified in China, especially in areas where H. longicornis is endemic.

Haemaphysalis longicornis

Reassortment

10.1016/j.ttbdis.2024.102375

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Reinforcement of innovation ability of graduate students and construction of a new teaching model of etiology.

Journal of Pathogen Biology (2009)

YU Ai-lian Zhong Zhang Guangfu Yu

To train innovation and creative thinking and practical ability of graduate students in etiology,the innovative training model based on selection,training and acquisition was established.

Etiology

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Aloe Polysaccharides Inhibit Influenza A Virus Infection—A Promising Natural Anti-flu Drug

Frontiers in Microbiology (2018)

Zhenhong Sun Cuilian Yu Wei Wang Guangfu Yu Tingting Zhang

Influenza A virus causes periodic outbreaks and seriously threatens human health. The drug-resistant mutants have shown an epidemic trend because of the abuse of chemical drugs. Aloe polysaccharides extracted from Aloe vera leaves have evident effects on the therapy of virus infection. However, the activity of APS in anti-influenza virus has yet to be investigated. Here, we refined polysaccharides from A. vera leaf. In vitro test revealed that APS could inhibit the replication of a H1N1 subtype influenza virus, and the most obvious inhibitory effect was observed in the viral adsorption period. Transmission electron microscopy indicated that APS directly interacted with influenza virus particles. Experiments on PR8 (H1N1) virus infection in mice demonstrated that APS considerably ameliorated the clinical symptoms and the lung damage of the infected mice, and significantly reduced the virus loads and mortality. Our findings provided a theoretical basis for the development of novel natural anti-influenza agents.

Aloe Vera

Antiviral drug

Virus quantification

10.3389/fmicb.2018.02338

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Citations (51)

Establishment of a model of bone deformity induced by deoxynivalenol in mice.

Journal of Pathogen Biology (2010)

Yu AiLian Zhu Xiao-ming Guangfu Yu Zhuang DongMing Xiaoxia Li

Objective To obtain a fetal mouse model of deoxynivalenol(DON) administration to detect skeletal malformations and embryotoxicity and provide an experimental platform for use in research on malformations induced by DON.Methods Pregnant mice were divided into a solvent control group(n=6) and DON experimental group(n=6).The experimental group was administered 2.5 mg/kg/day DON by intraperitoneal injection coinciding with days 7 to 10 of pregnancy;the total dose was 10 mg/kg.The solvent control group was injected with 30% propanediol.On day 18 of pregnancy,31 mouse fetuses were randomly selected from the experimental group and 63 were selected from the solvent control group.The soft tissue of the fetus was removed and bone and cartilage were double-stained.Development of bone was observed microscopically.Results There were multiple skeletal deformities in the experimental group.The most frequent malformations were sternal deformity(90%),thoracic deformity(90%),and limb deformities(54%).The contol group had natural development except for a few instances of sternal deformity(9%).Conclusion DON induced multiple bone deformities in mouse fetuses.Bone deformities induced by DON can serve as a model for research into congenital skeletal malformations.

Animal model

Intraperitoneal injection

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Medical undergraduates participating in teachers' scientific research

Chinese Journal of Medical Education (2010)

Xue-an Jing Zong Chuan-long Yongqi Fang Ping Wei Guangfu Yu

This paper discussed the practical effects for culturing hands-on ability, research ability and creative ability of medical undergraduates during their participation in research work undertaken by teachers. In this paper, we expounded the principle and main type that the students took part in the work,and investigated the students who took part in the work. Most students ( 80.0% ) thought this promoted their interest and approved their ability in research practice and consulting literature. Key words: Medical undergraduate; Scientific research; Exploration

10.3760/cma.j.issn.1673-677x.2010.03.032

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