Significant variations in leaf colours, pigment contents, and main taste compounds in young shoots from albino tea plants (Camellia sinensis) influence tea flavour. However, the seasonal metabolic pattern and molecular regulatory mechanism of these metabolites remain largely elusive. Herein, we conducted morphological, biochemical, metabolomic and transcriptomic analyses between an albino tea cultivar 'Zhonghuang 3' ('ZH3') and a green strain 'Tai cha 15' ('TC15') at four-time points (April 12, May 31, July 14, and August 17) to elucidate dynamic changes in these compounds and predict the relationships among transcription factors (TFs), target genes (TGs), and metabolite abundance. Generally, leaf colours and pigment contents were significantly lighter and lower, respectively, in 'ZH3' than in 'TC15' from spring to summer, but were subsequently similar. Compared to 'TC15', 'ZH3' had a lower and broader phenol/ammonia ratio as well as stable caffeine content and showed more significantly different metabolites and differentially expressed genes. The relationship between pigments, main taste compounds, and their biosynthetic genes, as well as TFs and their TGs, had genetic specificity. These results suggested that the biosynthesis of these compounds was probably both season- and variety-dependent. In total, 12 models of the TF-TG-metabolite regulatory network were proposed to uncover the biosynthetic and regulatory mechanisms of these metabolites in tea plants. A high correlation was observed between some structural genes and TFs with the accumulation of these metabolites. These findings provide novel insights into the regulatory mechanisms underlying accumulation of pigments and main taste compounds in tea plants.
Abstract According to the planting mode of broccoli and the growth characteristics of broccoli, a kind of high-gap agricultural chassis with structural reliability and strong stability was designed for broccoli picking. Firstly, the dynamics and kinematics of the chassis were analyzed, and then we analyzed the stability and completed the finite element analysis of the key components. Finally, the simulation and field test were carried out. Thus, the main performance parameters of the chassis are determined. The overall power, driving power consumption, maximum endurance time, maximum driving distance, maximum power storage, and maximum battery power were 5.959kw, 1.84kw, 3 h, 21 km, 5.63kwh, and 6.552kw respectively. The results of the slope climbing and ridge driving test were better than those of the simulation test. The actual slope climbing effect of the chassis accords with the calculation of theoretical value, and it can climb the soil slope of 25°. These findings proved the stability of the high-gap agricultural chassis in the field and indicated that the chassis can be applied to small fields in hilly and mountainous regions.
A gold nanoparticle (GNP) probe-based assay (GNPA) modified from the bio-barcode assay (BCA) was developed for ultrasensitive detection of ricin, a potential biothreat agent. In the GNPA, a chain of ricin was captured by a GNP probe coated with polyclonal antibodies and single-stranded signal DNA. A magnetic microparticle (MMP) probe coated with ricin A chain monoclonal antibody was then added to form an immuno-complex. After being magnetically separated, the immuno-complex containing the single-stranded signal DNA was characterized by PCR and real-time PCR. A detection limit of 10−2 fg/ml was determined for the ricin A chain; this is eight orders of magnitude more sensitive than that achieved with an ELISA and two orders more sensitive than that obtained with the BCA. The coefficients of variation (CV) of the intra- and inter-assay values ranged from 3.82–6.46%. The results here show that this novel assay is an ultrasensitive method for detection of ricin proteins and may be suitable for the ultrasensitive detection of other proteins.
Analyses of three B19V 1a/3b recombinant sequences. The left part of each panel was the results of bootscan analysis. At the right part of each panel were the neighbour-joining trees established on the basis of the fragments between breakpoints, as indicated by a bootscan plot of the sequence. (JPG 4401Â kb)
Xenotransplantation from animals has been considered to be a preferable approach to alleviate the shortage of human allografts. Pigs are the most suitable candidate because of the anatomical and physiological similarities shared with humans as well as ethical concerns. However, it may be associated with the risk of transmission of infectious porcine pathogens. Porcine endogenous retroviruses (PERVs) are of particular concern because they have been shown to infect human cells in vitro. To date, researches on the molecular characteristics and potential pathogenicity of PERV are still tenuous. In this report, an infectious replication competent clone of PERV from Wuzhishan pigs (WZSPs) in China was generated and characterized. This infectious clone will contribute to studies on PERV virology and control of PERV in xenotransplantation using Chinese miniature pigs.The proviral DNA of PERV from WZSPs was amplified in two overlapping halves. Then the two fragments were isolated, subcloned and fused to generate pBluescriptαSK+-WZS-PERV recombinant clones. Screened with RT-PCR, a molecular clone of PERV designated as WZS-PERV(2) was selected. Its infectivity and replication competency were characterized in HEK293 cells by PCR, real-time fluorescent quantitative RT-PCR, western blot, indirect immunofluorescence assay as well as sequence analysis.The ability of WZS-PERV(2) to infect human cells and produce infectious virions were shown after transfection of the clone into HEK293 cells and infection of PERV derived from this recombinant clone. The expression of Gag proteins were detected in HEK293 cells infected with the virus derived from the clone by the indirect immunofluorescence assay and western blot. The results of sequences analysis and comparison combined with the PCR based genotyping result demonstrated that the WZS-PERV(2) belonged to PERV-A subgroup. Compared with a previous proviral DNA clone of PERV (PERV-WZSP), G to A hypermutation occurred in the env gene of WZS-PERV(2) was found, whereas APOBEC proteins have the potential to inhibit the replication of a variety of retroviruses through a cDNA cytosine deamination mechanism, so we presumed these G to A hypermutation might be the contribution of porcine APOBEC3F.Altogether, an infectious replication competent clone of PERV from Chinese miniature pigs (WZSPs) termed WZS-PERV(2) was generated, characterized and sequenced.
The end-effector is an important part of the broccoli harvesting robot. Aiming at the physical characteristics of a large broccoli head and thick stem, a spherical cutting tool broccoli harvesting end-effector was designed in this study. First, the physical characteristics of broccoli were tested, and physical parameters such as the broccoli head diameter and stem diameter of broccoli were measured. The maximum cutting force of broccoli stems under different cutting angles was tested. Second, according to the physical characteristics and harvesting process of broccoli, the end-effector was designed, and the mathematical model of kinematics and dynamics was established. Based on the results of dynamic analysis, the end-effector rod was optimized, and the unilateral width of the slider was 40 mm, the length of the connecting rod was 120 mm, and the length of the crank was 42 mm. The mechanism needed an external driving force of 140.54 N to cut the broccoli stem. Therefore, a 32 mm cylinder with a load rate of 50% was selected as the power source. Finally, the feasibility of the broccoli harvesting end-effector was verified by the harvesting test. Experiments showed that the overall harvesting success rate of the end-effector is 93.3%, and the smoothness rate of the stem section is 83.3%. The harvesting performance of the broccoli end-effector was verified. This lays a foundation for agricultural robots to harvest broccoli. Keywords: harvesting robot, broccoli, end-effector, dynamic analysis, mechanism optimization DOI: 10.25165/j.ijabe.20241701.8110 Citation: Zhao X, Xu G J, Zhang P F, Yu G H, Xu Y D. Design and experimental study of the end-effector for broccoli harvesting. Int J Agric & Biol Eng, 2024; 17(1): 137-144.
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