Based on the common characteristic of severe acute respiratory syndrome (SARS) and highly pathogenic avian influenza and the mechanism of inflammation and fibrosis, it is speculated that there should exist a fundamental pathological rule that severe acute lung injury (ALI)-induced rapid pulmonary fibrosis is caused by various etiological factors, such as SARS coronavirus, H5N1-virus, or other unknown factors, and also by lipopolysaccharide (LPS), the most common etiological factor. The investigation employed intratracheally, and intraperitoneally and intratracheally applied LPS three-hit regimen, compared with bleomycin-induced chronic pulmonary fibrosis. Inflammatory damage and fibrosis were evaluated, and the molecular mechanism was analyzed according to Th1/Th2 balance, Sma- and MAD-related proteins (Smads) and signal transducer and activator of transcriptions (STATs) expression. The results suggested that rapid pulmonary fibrosis could be induced by ALI via LPS three-hits. The period from 3—7 days in the LPS group was the first rapid pulmonary fibrosis stage, whereas the second fast fibrosis stage occurred on days 14—21. Th2 cell polarization, Smad4 and Smad7 should be the crucial molecular mechanism of ALI-induced rapid fibrosis. The investigation was not only performed to establish a new rapid pulmonary fibrosis model, but also to provide the elicitation for mechanism of ALI changed into the rapid pulmonary fibrosis.
Objective:To explore the effect of Niupozhibao pellet(牛珀至宝微丸) on nitric oxide(NO) expression regulated by protein kinase C(PKC) induced by lipopolysaccharide.Methods:The septic shock model was induced by lipopolysaccharide and DGal.The serum NO was tested by Griess method.The expression of inducible nitric oxide synthase(iNOS) was measured by immunohistochemistry and Western blot.The activity of iNOS was assayed by isotope labeling.The effect of H7 and phorbol 12myristate 13acetate(PMA) on iNOS expression influenced by Niupozhibao pellet was observed.Results:Niupozhibao pellet could enhance the effect of H7 on iNOS expression and artery blood pressure and decreased NO level.The effect was counteracted by PMA.Conclusions:Niupozhibao pellet can regulate NO through PKC in septic shock.
To explore the effect of jianxin pinglu pill (JPP) on arrhythmia and aquaporin 4 (AQP4) in rats with myocardial ischemia/reperfusion (I/R) injury.The effects of JPP on arrhythmia, mortality and AQP4 on I/R injured rats model induced by blocking left coronary artery were observed using II lead of ECG, HE stain and AQP4 immunohistochemical stain.JPP showed significant effect in lowering the arrhythmia occurrence and mortality, reducing myocardial ischemic edema and injury, strengthening AQP4 expression in myocardial tissue.JPP has the effect of preventing I/R induced arrhythmia, it might be related with its action in up-regulating AQP4 expression level in myocardium and reducing the intracellular edema.
Objective:To observe the effect of Niupozhibao(NPZB) pellet on the expression of aquaporins in LPS-induced acute lung injury(ALI).Methods:LPS-induced shock models were established by applying the intravenous LPS 1.5 mg/kg and the intraperitoneal D-Galn 100mg/kg,and it was intervened with NPZB pellet.Then the lung injury was observed with HE staining,the pulmonary collagen fibers expression was detected with Van Gieson staining,and the expression of AQP-1 and AQP-5 was surveyed by immunohistochemical staining.Results:LPS induced severe ALI,the staining of collagen fibers in lung tissues increases dramatically,and the expression of AQP-1 and AQP-5 decreased.NPZB pellet lessened ALI and the expression of collagen fibers in lung tissues,and improve the expression of AQP-1 and AQP-5.Conclusion:The effect of NPZB pellet on LPS-induced ALI and fibrosis might be related to the regulation of AQP-1 and AQP-5 expression.
The variable stator vanes (VSV) is prone to happen joint faults such as wear and stagnation due to complex structure and a large number of kinematic pairs. Based on the circumferential spatial distribution characteristics of the joints around actuation ring, this paper establishes a single-stage ring torsional inertia-spring model that considers nonlinearity caused by joint faults. Refer to a real aero-engine's VSV structure, the system parameters of the model are obtained by simplifying, and then the motion differential equation of the system is obtained. The torsional vibration response of each vane of the system is obtained by the newmark$-\beta$ integral method. This paper also developed a diagnosis methodology based on the nonlinear output frequency response functions (NOFRFs), and established a second-order optimal weighted contribution rate indices Rm. Based on the torsional vibration response of each vane, the corresponding Rm is extracted. The asymmetry characteristic of the Rm around the single-stage actuation ring with fault to the excitation vane is established, and the influence of the excitation intensity and other factors on the asymmetry are analyzed. The results show that the diagnosis methodology proposed in this paper can effectively detect joint faults of VSV in aero-engine.
[ Objective ] To investigate the mechanism of Jianxin Pinglu Pill (JPP) in counteracting arrhythmia. [Methods] Ninety rats were randomized into 3 groups: model (normal saline), high-dose JPP (1.6 g/kg) and low-dose JPP (0.8g/kg) . The treatment lasted 7 days. Rat models with myocardial ischemia /repermsion were established by blocking left coronary artery. Ⅱ lead of electrocardiogram was recorded, connexin 43 (Cx 43) was detected by immunohistochemical assay. The effect of JPP on incidence of arrhythmia, death rate and the expression of connexin 43 in the myocardium was observed. [Results] JPP could decrease the incidence of arrhythmia and death rate obviously and enhance the expression of connexin 43 in myocardium. [ Conclusion ] JPP can counteract the damage of myocardial ischemia /reperfusion to enhance Cx 43 expression, and this is one of its possible mechanism.
Abstract In order to study the quantitative structure-activity relationship (QSAR) of the balance activity (p H ) for 30 benzodiazepinooxazole derivatives to male mice, the molecular electronegativity distance vector ( M D ) of above compounds was calculated by program according to molecular topological environment in this paper. The five-variable ( M 6, M 46 , M 25, M 63, M 70 ) QSAR model of p H for above compounds was constructed by using leaps-and-bounds regression method. The result demonstrates that the model is robustness and good prediction ability by using R cv 2 , F tests. The M 6, M 46 , M 25, M 63 and M 70 were used as the input neurons of artificial neural network (ANN), and a 5:3:1 network architecture was employed. A satisfying BP-pH model could be constructed with the back-propagation algorithm, with the correlation coefficient ( R 2 ) and the standard error( S D ) being 0.928 and 0.117, respectively, showing that the relationship between p H and these structural parameters has a good nonlinear correlation. Form the three parameters of the model, it is known that the dominant influence factors of increased balance activity are the microscopic fragments: –CH 3 , –CH 2 –, >C<, –NH–, –N< and –X in the molecules.
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