Objective To observe the plasma vasoactive intestinal polypeptide(VIP) concentration in infants with cytomegalovirus(CMV) hepatitis and to explore the correlation between plasam VIP level and liver injury,cholestasis or biliary atresia. Methods Sixty-two infants with CMV hepatitis in Nanjing children′s hospital from Aug.2008 to May 2009 were divided into 4 groups,in which hepatits group contained 13 cases(male 8 cases,female 5 cases),cholestasis group contained 15 cases(male 8 cases,female 7 cases),hepatitis and cholestasis group contained 20 cases(male 11 cases,female 9 cases);and biliary atresia group contained 14 cases(male 7 cases,female 7 cases).Healthy control group contained 14 cases(male 8 cases,female 6 cases).Breast milk jaundice group contained 15 cases(male 7 cases,female 8 cases).The plasma VIP concentrations of infants with CMV hepatitis,and breast milk jaundice,healthy infants(healthy control group)were measured by using radioimmunoassay. Results VIP concentration in plasma of infants in hepatitis group [(61.75±8.30) ng/L] was higher than that in healthy control group [(41.83±9.30) ng/L P 0.05];VIP concentration in plasma of infants in cholestasis group [(63.73±3.54) ng/L] was obviously higher than that in healthy control group(P 0.01).And there was no significant difference between breast milk jaundice group and healthy control group [(35.16±10.14) ng/L P 0.05].The concentration of plasma VIP in hepatitis and cholestasis group[(53.62±4.00) ng/L] was significantly higher than that in healthy control group(P 0.05);The plasma VIP concentration in biliary atresia group[(20.27±9.00) ng/L] was significantly less than that in healthy control group(P 0.01). Conclusions Increased plasma VIP level is the result of liver injury and also the reason of promoting the formation of cholestasis in infants with CMV hepatitis.Increased plasma VIP level of infants with biliary atresia possibly reduces hepatobiliary injury by inhibiting hepatobiliary autoimmune responses,which shows the potential therapeutic value.
Objective To explore the preventive and therapeutic effects and mechanism of curcumin on gastric stress ulcer in rats.Methods Forty male SD rats were divided into 4 groups randomly: normal control group,ulcer model group,curcumin-treated group and omeprazole-treated group.Water immersion-restraint stress model was performed,and the gastric mucosa ulcer index(UI),gastric juice pH and H+,K+-ATPase activity were measured.The structural changes in gastric mucosa were observed by histopathologically.The results were analyzed with the Software of SPSS 13.0.Results Compared with normal control group,the UI(35.63±3.34) and H+,K+-ATPase acti-vity(9.45±0.34) mmol/(g·h) increased significantly(Pa0.01),and pH value(1.35±0.09) decreased significantly(P0.01) in ulcer model group.Gastric mucosal hemorrhage and ulcer were observed under light microscope in ulcer model group.Compared with ulcer mo-del group,the UI(15.13±2.42) and H+,K+-ATPase activity(8.13±0.59) mmol/(g·h) decreased significantly(Pa0.01),and pH value(1.81±0.23) increased significantly(P0.01) in curcumin-treated group.No gastric ulcer but lower-grade injury were observed.The UI,pH value,H+,K+-ATPase activity and structural changes in gastric mucosa of curcumin-treated group were no significant deviation compared with omeprazole-treated group(Pa0.05).The gastric juice pH value and H+,K+-ATPase activity demonstrated negative correlation(r=-0.884 P0.01).Conclusions Curcumin can obviously reduce gastric mucosal injury,inhibit H+,K+-ATPase activity,reduce gastric acid secretion and it has a good preventive and therapeutic effect on gastric ulcer.
Objective
To investigate Helicobacter pyloric (Hp) infection in children who underwent gastroscopy in Nanjing area.
Methods
From Jan.2001 to Dec.2010, 2 990 pediatric patients with gastrointestinal symptoms who underwent endoscopy and Hp rapid urease test of gastric mucosa specimens.
Results
There were 2 990 patients who underwent gastroscopy during the 10 years including 1 718 cases of male and 1 272 cases of female.The rate of Hp infection was 53.8%(924/1 718 cases) and 52.4%(667/1 272 cases) in male and female, respectively.There was no significant difference (χ2=0.532, P>0.05). From Jan.2001 to Dec.2005, 611 patients were tested and the rate of Hp infection was 66.9%(409/611 cases). From Jan.2006 to Dec.2010, 2 379 patients were tested and the rate of Hp infection was 49.7%.There was significant difference(χ2=58.13, P=0.01) between the 2 groups.The rate of Hp infection were 49.6%, 48.1%, 55.2%, 60.0% in 0-6 years group, >6-9 years group, >9-12 years group, and>12 years group, respectively.There was significant difference among the 4 different age groups(χ2=23.66, P<0.01). The rate of Hp infection in nodular gastritis(61.4%) was higher than that in chronic superficial gastritis (50.6%). There was significant difference between the 2 groups(χ2=7.42, P<0.01). The rate of Hp infection in allergic purpura (46.2%)was higher compared with the children with normal gastroscopy results(15.9%). There was significant dif-ference between the 2 groups(χ2=10.19, P<0.01).
Conclusions
There is gradually downward trend by year in the rate of Hp infection of children who underwent gastroscopy in Nanjing area by year.The rate of Hp infection is increa-sing with the age after the age of 6 years.Hp infection may play a role in the nodular gastritis and allergic purpura.
Key words:
Gastroscopy; Helicobacter pylori; Child
Objective
To explore the clinical manifestations and endoscopic characteristics of eosinophilic gastroenteritis(EG) with massive ascites as the initial symptom and accumulate the experiences of EG for early diagnosis and treatment.
Methods
Clinical characteristics, laboratory examination, endoscopic features and treatment methods of 6 cases of pediatric patients with EG from Nanjing Children's Hospital Affiliated to Nanjing Medical University from May 2009 to Dec.2012 were analyzed.
Results
The main clinical manifestations of 6 cases with EG serous type were abdominal distension, ascites.Peripheral blood eosinophil(EOS) counts was increased significantly(5.1×109/L-20.6×109/L). A large number of EOS were found in ascitic fluid of 6 patients.Endoscopic manifestations was gastric mucosal hyperemia and edema in the all of 6 patients, and furthermore, gastric mucosa erosion in 2 cases, esophagus mucosa rough edema in 1 case, duodenal mucosal hyperemia and edema in 3 cases, duodenal nodular hyperplasia in 1 case.The mucosa pathological examination of 6 cases showed eosinophilia.Symptoms in patients were relieved after avoiding suspicious food and oral corticosteroids treatment after 1 week.
Conclusions
EG serous type patients in children are massive ascites as the initial symptom.Ascitic fluid and peripheral blood EOS counts are increased significantly and endoscopic feature is nonspecific, mucosal hyperemia, edema, erosions, nodular hyperplasia.EOS infiltration is observed in the gastrointestinal mucosa propria by multipoint biopsy.Therapeutic effect of diet therapy and the treatment of glucocorticoid is good.
Key words:
Eosinophilic gastroenteritis; Ascites; Child
Objective To investigate the clinical effect of urine gas chromatography-mass spectrometry(GC-MS) analysis in the dia-gnosis of inherited metabolic liver disease in children.Methods Fifty-six cases of inpatients,with the clinical manifestations of jaundice,clinical manifestation and growth retardation blood test showed a high conjugated bilirubin accompany with hyperammonemia,hypoglycaemia,metabolic acidosis and hyperlipidemia.All 56 cases undergone the analysis of urine GC-MS,and the data were analyzed by software of inherited metabolic liver disease.Results Ten cases were highly suspected for Citrin deficiency,and 3 cases were confirmed for Citrin deficiency among those 6 cases undergone genetic analysis;but the other 4 cases were found no jaundice after 1 month medium chain triglycerides-enriched and lactose-free formula theatment.Three cases were highly suspected for galactosemias,and 2 cases of them were confirmed by blood test,the jaundice disappeared after 3 months with lactose-free formula treatment.Another included 1 case of tyrosinemias,and 2 cases of disorders of lipoprotein and lipid metabolism.Conclusions Urine GC-MS analysis is a convenient,simply and no-injury algorithms for the screening of inherited metabolic liver disease in children.The clinical manifestation of cholestasis,feeding difficulty,growth retardation,hepatosplenomegaly,hypoglycaemia,anemia and aminoacidemia may hint for inherited metabolic liver disease in children.
Cross-linked polyimide aerogels are viable approach to higher temperature, flexible insulation for inflatable decelerators. Results indicate that the all-polyimide aerogels are as strong or stronger than polymer reinforced silica aerogels at the same density. Currently, examining use of carbon nanofiber and clay nanoparticles to improve performance. Flexible, polyimide aerogels have potential utility in other applications such as space suits, habitats, shelter applications, etc. where low dusting is desired
Objective To determine plasma somatostatin(SST)concentration in infants with cytomegalovirus(CMV)hepatitis,and investigate the correlation between plasma SST and liver injuries,cholestasis,biliary atresia.Methods Sixty-two infants with CMV hepatitis and 15 children with breast milk jaundice admitted in Nanjing Children's Hospital from August 2008 to May 2009 were recruited,and 14 healthy infants were studied as control.The patients were clinically diagnosed with CMV hepatitis based on the following conditions:CMV-DNA level reached diagnostic value in serum or urine,serum CMV-IgM was positive,and antibodies of hepatitis A,B,C,D,and hepatitis E virus was negative.Serum alanine aminotransferase(ALT)level and serum direct bilirubin level(DB)were higher than those of healthy infants.The patients were divided into 4 groups:hepatitis group(n = 13,including 8 male and 5 female),cholestasis group(n = 15,including 8 male and 7 female),hepatitis and cholestasis group(n = 20,including 11 male and 9 female),and biliary atresia group(n = 14,including 7 male and 7 female).Plasma SST concentration was measured by radioimmunoassay.Results Plasma SST concentration was higher in hepatitis group(55.52 ± 4.32 ng/L),and lower in cholestasis group(13.29 ± 3.54 ng/L)than that in normal control group(25.40 ± 4.23 ng/L)(P 0.01,P 0.05,respectively).The difference between breast milk jaundice group and normal control group was not significant(24.10 ± 7.90 ng/L vs 25.40 ± 4.23 ng/L,P 0.05).The concentration of plasma SST was significantly higher in hepatitis and cholestasis group(49.24 ± 10.41 ng/L),but lower in biliary atresia group(18.19 ± 4.03 ng/L),compared with normal control group(P 0.01,P 0.05,respectively).Conclusions This study suggested that elevated plasma SST level might reflect liver injury,and decreased plasma SST level may be closely correlated with increased serum DB level.
Objective To determine the changes of interleukin-12(IL-12) and IL-12 mRNA in gastric mucosa of children with helicobacer pylori (Hp) infection,and to study the effects of Hp infection on the expression levels of IL-12 and IL-12 mRNA,and to evaluate its possible roles in the pathogenesis of gastric mucosal inflammation in Hp related gastroduodenal diseases.Methods Biopsy specimens were taken from the antral mucosa on endoscopy in patients with or without Hp infection, which were diagnosed by urease test and Giemsa staining. The expression levels of IL-12 and IL-12 mRNA in gastric mucosa were determined by ELISA and RT-PCR.Results Inflammation of gastric antral mucosa was more severe in Hp-positive mucosa .The expression levels of IL-12 and IL-12 mRNA in Hp-positive mucosa were (66.42±15.15) ng/g and (59.21±15.03)%,which were more than those in (Hp-negative )(22.22±8.79) ng/g and (17.94±7.39)%(P0.01).Conclusions The expression levels of IL-12 and IL-12 mRNA are higher in Hp-positive gastritis. IL-12 may play a pivotal role in the Hp associated gastric disease.
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