Peiyue Dong

Northwest A&F University

Author Statistics

Papers

Citation

H-Index

i-10 index

Research Trends

Author Order

Document Type

Co-Authors

Gongshe Yang

Northwest A&F University

Shiduo Sun

Northwest A&F University

Zhenyu Zhang

First Affiliated Hospital of Henan University

Yin Mai

Northwest A&F University

Xiaomin Ning

Yangzhou University

Yanjie Li

Peking University

Shuai Liu

Zhengzhou University

Yang Qiu

Qilu Hospital of Shandong University

Guiyan Chu

Northwest A&F University

Hao Yang

Shanghai Jiao Tong University

Cooperative Institutions

Northwest A&F University

Sun Yat-sen University

Sichuan University

Dalian Medical University

Agriculture and Forestry University

Ministry of Agriculture and Rural Affairs

Hebei Medical University

West China Hospital of Sichuan University

First Affiliated Hospital of Zhengzhou University

Shandong University

Author Statistics

Papers

Citation

H-Index

i-10 index

Research Field

[Over-expressed MiR-103 promotes porcine adipocyte differentiation].

PubMed (2012)

Meihang Li Yang Qiu Shuai Liu Peiyue Dong Xiaomin Ning

To clarify the function of miR-103 in the differentiation of porcine preadipocyte, we carried out real-time PCR to detect the expression pattern of miR-103 during adipogenesis, and clarified its expression tendency through cell differentiation. Then we used adenovirus that overexpressed miR-103 to infect porcine preadipocyte. Subsequently, mRNA and protein expression of adipogenesis marker--PPARgamma and aP2 was analyzed by real-time PCR and Western blotting. At last, Oil-Red O staining was used to detect lipids accumulation in the 8th day after adipogenic inducement. The expression of miR-103 increased during adipocyte differentiation; compared with the control, the preadipocyte infected by pAd-miR-103 had an elevated expression level of adipocyte marker gene PPARgamma, aP2, and obvious lipid droplet was seen in the 8th day after adipogenic inducement. These results showed that miR-103 can enhance adipogenesis in primary cultured porcine adipocytes.

Oil Red O

Lipid droplet

Source

Cite

Citations (8)

miR-191 May Regulate Pig Preadipocyte Differentiation by Targeting The Transcription Factor C/EBPβ

ACTA AGRONOMICA SINICA (2013)

Shuai Liu Xiaomin Ning Meihang Li Yang Qiu Yanjie Li

10.3724/sp.j.1206.2012.00267

Cite

Citations (1)

BMP and activin membrane-bound inhibitor (BAMBI) inhibits the adipogenesis of porcine preadipocytes through Wnt/β-catenin signaling pathway

Biochemistry and Cell Biology (2014)

Yin Mai Zhenyu Zhang Hao Yang Peiyue Dong Guiyan Chu

The process of differentiation from preadipocytes to adipocytes contributes to adipose tissue expansion in obesity. Blocking adipogenesis may be conducive to the etiology of obesity-related diseases. BMP and activin membrane-bound inhibitor (BAMBI) is a transmembrane protein, which was identified as a target of β-catenin in colorectal and hepatocellular tumor cells. However, whether BAMBI affects adipogenesis by Wnt/β-catenin signaling remains to be explored. In this study, we distinguish BAMBI as an inhibitor of preadipocytes differentiation. We found that BAMBI was downregulated during preadipocytes differentiation. Knockdown of BAMBI increased adipogenesis and blocked Wnt/β-catenin signaling by repressing β-catenin accumulation. In BAMBI overexpression cells, lipid accumulation was reduced by promoting nuclear translocation of β-catenin. Lithium chloride (LiCl) is an activator of Wnt/β-catenin signaling, which is an inhibitor of glycogen synthetase kinase-3 (GSK-3), maintaining the stability of β-catenin in cytosolic. We showed BAMBI strengthened the anti-adipogenic effects of LiCl. In addition, the results indicated that BAMBI was upregulated by β-catenin. These observations illuminated that BAMBI inhibits adipogenesis by a feedback loop (BAMBI→β-catenin nuclear translocation→BAMBI), which forms with Wnt/β-catenin signaling.

Beta-catenin

10.1139/bcb-2014-0011

Cite

Citations (36)

Inhibition of the pre-adipocyte differentiation in rat (Rattus norvegicus) caused by over-expression of miR-128a.

Journal of Pharmaceutical and Biomedical Sciences (2013)

Yang Qiu Shuai Liu Xiaomin Ning Peiyue Dong Cheng Jia

Source

Cite

Citations (0)

MiR-15a/b promote adipogenesis in porcine pre-adipocyte via repressing FoxO1

Acta Biochimica et Biophysica Sinica (2014)

Peiyue Dong Yin Mai Zhenyu Zhang Lin Mi Guofang Wu

Abstract Diabetes and many other metabolism syndromes are closely related to obesity. To reveal the underlying mechanism of fat deposition, an increasing number of studies are focusing on the functions of miRNAs during adipocytes development. Previous studies have proved that miR-15a/b play important roles in multiple physiological processes; however, their functions during adipogenesis remain unclear. To reveal this, we detected the expression profiles of miR-15a/b during adipogenesis in porcine pre-adipocyte, and found that their expression levels increased in the early stage of adipocyte differentiation and dropped after day 4. Moreover, over-expression of miR-15a/b in porcine pre-adipocytes promoted adipocyte differentiation and lipid accumulation. Target genes of miR-15a/b were predicted and examined, which revealed that Forkhead box protein O1 (FoxO1) is the target gene of miR-15a/b. The inhibition of FoxO1 expression level caused by miR-15a/b over-expression had a positive effect on adipogenesis. Thus, we conclude that miR-15a/b promote adipogenesis in porcine pre-adipocyte via repressing FoxO1.

FOXO1

10.1093/abbs/gmu043

Cite

Citations (40)

[BAMBI inhibits porcine preadipocyte differentiation by facilitating ERK1/2 phosphorylation].

PubMed (2014)

Yin Mai Zhenyu Zhang Peiyue Dong Hao Yang Gongshe Yang

To study the role of BAMBI in adipogenesis, we constructed lentivirus interfering vector targeting on porcine BAMBI, packaged and infected the porcine preadipocyte. The differentiation state of preadipocyte was detected by Oil Red O staining and Oil Red O extraction assay and the expression levels of adipogenic marker genes were detected by Real-time qPCR and Werstern bloting. Results show that BAMBI expression was significant decreased after lentivirus infection, which was repressed more than 60% by shRNA2. Moreover, knockdown BAMBI increased the lipid accumulation of porcine preadipocyte and improved the expression of PPARγ (peroxisome proliferator-activated receptorγ) and ap2 (adipocyte protein 2). In summary, these data indicated that BAMBI inhibited adipocyte differentiation by facilitating the phosphorylation of ERK1/2.

Oil Red O

Source

Cite

Citations (2)

CTSB promotes porcine preadipocytes differentiation by degrading fibronectin and attenuating the Wnt/β-catenin signaling pathway

Molecular and Cellular Biochemistry (2014)

Zhenyu Zhang Yin Mai Hao Yang Peiyue Dong Xueli Zheng

10.1007/s11010-014-2111-6

Cite

Citations (15)