Haishan Zhu

Nanchang Hangkong University

Author Statistics

Papers

Citation

H-Index

i-10 index

Research Trends

Author Order

Document Type

Co-Authors

Minghua Deng

Yunnan Agricultural University

Jinfen Wen

Kunming University of Science and Technology

Junheng Lv

Yunnan Agricultural University

Kai Zhao

Shanxi Agricultural University

Jinlong Huo

Fujian Agriculture and Forestry University

Xuexiao Zou

Hunan Agricultural University

Zhuqing Zhang

Shandong University of Technology

Yanling Yue

Yunnan Agricultural University

Zhengan Yang

Yunnan Agricultural University

Shurui Jiang

Yunnan Agricultural University

Cooperative Institutions

Yunnan Agricultural University

Microsoft (United States)

Chinese Academy of Sciences

Microsoft (Finland)

Microsoft Research (United Kingdom)

Nanchang Hangkong University

Zero to Three

Nanjing Institute of Vegetable Science

Hunan Academy of Agricultural Sciences

University of Chinese Academy of Sciences

Author Statistics

Papers

Citation

H-Index

i-10 index

Research Field

Study of Uneven Distribution in Parallel Petroleum Processing Pipes

Volume 3: Operations, Monitoring, and Maintenance; Materials and Joining (2016)

Renwei Liu Haishan Zhu Qingping Li Yufei Wan Kaifeng Fan

The aim of this article is to investigate uneven distribution of oil-gas two phase flow in parallel petroleum processing pipelines. On-site analysis on BZ35-2 central platform A and SZ36-1 central platform N/O (two typical platforms in China’s Bohai Bay) shows that uneven distribution is originated mainly by two sources: flow rate difference and dryness difference. A 3-dimensional numerical model of two-phase flow in T-junction before parallel processing units was built. Flow and dryness distribution under different operating conditions were simulated. It is demonstrated that unevenness of flow rate grows worse as the total flow rate increases or operating pressure difference between parallel units becomes larger. Moreover, unevenness of dryness is mainly caused by phase split in a tee. It can be concluded that the phase split will be more obvious when parallel units are located at different heights or gas volume fraction of feed stream and inlet flow rate is small. Besides, flow rate distribution has an effect on dryness distribution. There is a specific flow ratio that will cause the most serious phase split. Finally, according to the conclusions, modification scheme for BZ35-2 central platform A piping layout was proposed. And this work may provide some guidance for process design and practical operation of parallel units.

Dryness

Multiphase flow

10.1115/ipc2016-64262

Cite

Citations (0)

Flower transcriptome dynamics during nectary development in pepper (Capsicum annuum L.)

Figshare (2021)

Minghua Deng Kai Zhao Junheng Lv Jinlong Huo Zhuqing Zhang

Abstract The measurement of gene expression can provide important information about gene function and the molecular basis for developmental processes. We analyzed the transcriptomes at three different developmental stages of pepper flower [sporogenous cell division, stage (B1); pollen mother cell meiosis, stage (B2); and open flower (B3)]. In the cDNA libraries for B1, B2, and B3: 82718, 77061, and 91491 unigenes were assembled, respectively. A total of 34,445 unigene sequences and 128 pathways were annotated by KEGG pathway analysis. Several genes associated with nectar biosynthesis and nectary development were identified, and 8,955, 12,182, and 23,667 DEGs were identified in the B2 vs B1, B3 vs B1, and B3 vs. B2 comparisons. DEGs were involved in various metabolic processes, including flower development, nectar biosynthesis, and nectary development. According to the RNA-seq data, all 13 selected DEGs showed similar expression patterns after q-PCR analysis. Sucrose-phosphatase, galactinol-sucrose galactosyltransferase, and sucrose synthase played very important roles in nectar biosynthesis, and CRABS CLAW could potentially be involved in mediating nectary development. A significant number of simple sequence repeat and single nucleotide polymorphism markers were predicted in the Capsicum annuum sequences. The new results provide valuable genetic information about flower development in pepper.

Capsicum annuum

10.6084/m9.figshare.14270471

Cite

Citations (0)

cDNA cloning, sequence characterization and expression analyses of a novel gene-TPI from pepper cytoplasmic male sterility (CMS)

Journal of Medicinal Plants Research (2012)

Minghua Deng Jinfen Wen Jinlong Huo Haishan Zhu Xiongze Dai

The complete coding sequence (CDS) of the gene triose phosphate isomerase (TPI) was amplified using the reverse transcriptase polymerase chain reaction based on the conserved sequence information of some Solanaceae plants and known highly homologous pepper ESTs. The results showed the pepper TPI gene encodes a protein of 254 amino acids that has high homology with the proteins of six other species, Solanum tuberosum(95%), Arabidopsis thaliana (87%), Ricinus communis, Vitis vinifera and Oryza sativa(87%) and Zea mays (85%). The pepper TPI gene was expressed in root, stem, leaf, flower, pericarp, placenta and seed. At the abortion stages, the ATP and NADH pool in male-sterile line were distinctly lower than those in the maintainer; The activities and expression levels of TPI in anthers of sterile line were obviously reduced, while levels in F1hybrid and maintainers kept normal, which indicated that stable transcripts of TPI are beneficial to keep the energy metabolism with a normal level. But when the restorer gene was transferred into CMS line, activities and expression level of TPI could be regulated by the restorer gene and kept stably. The restorer gene likely plays an important role in keeping the balance of the energy metabolism with a normal level during the microspore development. Key words: Pepper (Capsicum annuum L.), triose phosphate isomerase, cloning, characterization, cytoplasmic male sterility.

Cytoplasmic male sterility

Chromoplast

10.5897/jmpr11.1703

Cite

Citations (8)

Construction of a cDNA library from Phytophthora infestans infected tomato leaves and sequence analysis of the Expressed Sequenced Tags

Zhongguo Nongye Daxue xuebao (2010)

Yang Ting-ting Kai Zhao Haishan Zhu

A cDNA library was constructed to investigate late blight disease-resistant mechanism and initially obtained some disease resistant related Expressed Sequenced Tags(ESTs) of tomato(Lycopersicon esculentum Mill.) leaves infected by late blight disease(Phytophthora infestans) using SMART(Switching mechanism at 5′ end of RNA transcript) technique.The library had a high titer of 2.3×109 pfu/ml with a recombination rate of 96%.The inserted cDNA fragments ranged from 0.1～2.0 kb by PCR detection.After differential screening on the cDNA library,107 ESTs were identified and their GenBank accession numbers were GT742146-GT742150 and GT865998-GT866099.BLAST analysis showed that:75 ESTs presented significant similarity to the genes identified from other species.It was found that 42.7% of the ESTs involved in energy metabolism,10.7% in cellular life activities and signal conduction,9.3% in gene transcription and translation and 25.3% of the ESTs was involved in resistance.32 ESTs with low similarity or no match to the known genes,and they may contain some unidentified functions.The cDNA library enjoyed good quality and rich information,and the information generated in this study had established a basis for screening candidate genes involved in tomato disease-resistant.

Phytophthora infestans

Lycopersicon

Source

Cite

Citations (0)

Integrative analysis of the transcriptome and metabolome reveals the response mechanism to tomato spotted wilt virus

Horticultural Plant Journal (2022)

Junheng Lv Minghua Deng Zuosen Li Haishan Zhu Ziran Wang

Tomato spotted wilt virus (TSWV) is an important virus that has rapidly spread throughout the world. TSWV seriously hinders the production of tomato (Solanum lycopersicum) and other plants. In order to discover more new genes and metabolites related to TSWV resistance in tomato plants, the genes and metabolites related to the resistance of tomato plants inoculated with TSWV were identified and studied herein. The tomato TSWV-resistance line YNAU335 (335) and TSWV-susceptible lines NO5 and 96172I (961) were used as the transcriptome and metabolome research materials. Transcriptomic and metabolomic techniques were used to analyze the gene and metabolite response mechanisms to TSWV inoculation. A total of 3 566, 2 951, and 2 674 differentially expressed genes (DEGs) were identified in lines 335, NO5, and 961, respectively. Meanwhile, 208, 228, and 273 differentially accumulated metabolites (DAMs) were identified in lines 335, NO5, and 961, respectively. In line 335, the number of DEGs was the highest, but the number of DAMs was lowest. Furthermore, 903 DEGs and 94 DAMs were common to the response to TSWV in the three inbred lines. The 903 DEGs and 94 DAMs were mainly enriched in the plant hormone signal transduction and flavonoid synthesis pathways. In addition, many nucleotide-binding site-leucine-rich repeat genes and transcription factors were found that might be involved in the TSWV response. These results provide new insights into TSWV resistance mechanisms.

Metabolome

10.1016/j.hpj.2022.12.008

Cite

Citations (11)

Pushing the Limits of Narrow Precision Inferencing at Cloud Scale with Microsoft Floating Point

Neural Information Processing Systems (2020)

Bita Darvish Rouhani Daniel Lo Ritchie Zhao Ming Liu Jeremy Fowers

In this paper, we explore the limits of Microsoft Floating Point (MSFP), a new class of datatypes developed for production cloud-scale inferencing on custom hardware. Through the co-evolution of hardware design and algorithms, MSFP achieves accuracy comparable to or better than industry standards Bfloat16 and INT8 at 3x and 4x lower cost, respectively. MSFP incurs negligible impact to accuracy (

Double-precision floating-point format

Source

Cite

Citations (44)

Flower transcriptome dynamics during nectary development in pepper (Capsicum annuum L.)

Genetics and Molecular Biology (2020)

Minghua Deng Kai Zhao Junheng Lv Jinlong Huo Zhuqing Zhang

The measurement of gene expression can provide important information about gene function and the molecular basis for developmental processes. We analyzed the transcriptomes at three different developmental stages of pepper flower [sporogenous cell division, stage (B1); pollen mother cell meiosis, stage (B2); and open flower (B3)]. In the cDNA libraries for B1, B2, and B3: 82718, 77061, and 91491 unigenes were assembled, respectively. A total of 34,445 unigene sequences and 128 pathways were annotated by KEGG pathway analysis. Several genes associated with nectar biosynthesis and nectary development were identified, and 8,955, 12,182, and 23,667 DEGs were identified in the B2 vs B1, B3 vs B1, and B3 vs. B2 comparisons. DEGs were involved in various metabolic processes, including flower development, nectar biosynthesis, and nectary development. According to the RNA-seq data, all 13 selected DEGs showed similar expression patterns after q-PCR analysis. Sucrose-phosphatase, galactinol-sucrose galactosyltransferase, and sucrose synthase played very important roles in nectar biosynthesis, and CRABS CLAW could potentially be involved in mediating nectary development. A significant number of simple sequence repeat and single nucleotide polymorphism markers were predicted in the Capsicum annuum sequences. The new results provide valuable genetic information about flower development in pepper.

KEGG

10.1590/1678-4685-gmb-2018-0267

Cite

Citations (5)

Orf165 is associated with cytoplasmic male sterility in pepper

Figshare (2022)

Jinfen Wen Kai Zhao Junheng Lv Jinlong Huo Zi-ran Wang

Abstract Cytoplasmic male sterility (CMS) is a maternally inherited trait that derives from the inability to produce functional pollen in higher plants. CMS results from recombination of the mitochondrial genome. However, understanding of the molecular mechanism of CMS in pepper is limited. In this study, comparative transcriptomic analyses were performed using a near-isogenic CMS line 14A (CMS-14A) and a maintainer line 14B (ML-14B) as experimental materials. A total of 17,349 differentially expressed genes were detected between CMS-14A and ML-14B at the PMC meiosis stage. Among them, six unigenes associated with CMS and 108 unigenes involved in energy metabolism were identified. The gene orf165 was found in CMS-14A. When orf165 was introduced into ML-14B, almost 30% of transgenic plants were CMS. In addition, orf165 expression in transgenic CMS plants resulted in abnormal function of some genes involved in energy metabolism. When orf165 in transgenic CMS plant was silenced, the resulted orf165-silenced plant was male fertile and the expression patterns of some genes associated with energy metabolism were similar to ML-14B. Thus, we confirmed that orf165 influenced CMS in pepper.

Cytoplasmic male sterility

10.6084/m9.figshare.19920832

Cite

Citations (0)

Ethylene-Inducible AP2/ERF Transcription Factor Involved in the Capsaicinoid Biosynthesis in Capsicum

Frontiers in Plant Science (2022)

Jinfen Wen Junheng Lv Kai Zhao Xiang Zhang Zuosen Li

Ethylene is very important in the process of plant development and regulates the biosynthesis of many secondary metabolites. In these regulatory mechanisms, transcription factors (TFs) that mediate ethylene signals play a very important role. Capsaicinoids (CAPs) are only synthesized and accumulated in Capsicum species, causing their fruit to have a special pungent taste, which can protect against attack from herbivores and pathogens. In this study, we identified the TF CcERF2, which is induced by ethylene, and demonstrated its regulatory effect on CAPs biosynthesis. Transcriptome sequencing analysis revealed that the expression patterns of CcERF2 and multiple genes associated with CAPs biosynthesis were basically the same. The spatiotemporal expression results showed CcERF2 was preferentially expressed in the placenta of the spicy fruit. Ethylene can induce the expression of CcERF2 and CAPs biosynthesis genes (CBGs). CcERF2 gene silencing and 1-methylcyclopropene (1-MCP) and pyrazinamide (PZA) treatments caused a decrease in expression of CBGs and a sharp decrease in content of CAPs. The results indicated that CcERF2 was indeed involved in the regulation of structural genes of the CAPs biosynthetic pathway.

10.3389/fpls.2022.832669

Cite

Citations (14)

Mapping and functional characterization of the tomato spotted wilt virus resistance gene SlCHS3 in Solanum lycopersicum

Molecular Breeding (2022)

Junheng Lv Minghua Deng Shurui Jiang Haishan Zhu Zuosen Li

Candidate gene

Tospovirus

10.1007/s11032-022-01325-5

Cite

Citations (4)