Sung‐Myung Kang

Research Field

A novel angiotensin I-converting enzyme (ACE) inhibitory peptide from a marine Chlorella ellipsoidea and its antihypertensive effect in spontaneously hypertensive rats

Process Biochemistry (2012)

Seok‐Chun Ko Nalae Kang Eun-A. Kim Min Kang Seung‐Hong Lee

Pepsin

IC50

Proteolytic enzymes

Proteolysis

Bromelain

10.1016/j.procbio.2012.07.015

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Citations (169)

Quercitrin protects against oxidative stress-induced injury in lung fibroblast cells via up-regulation of Bcl-xL

Journal of Functional Foods (2012)

Young-Min Ham Weon‐Jong Yoon Soo-Yeong Park Gwan-Pil Song Yong‐Hwan Jung

The cytoprotective effect of quercitrin (QR) against oxidative stress induced cell damage by hydrogen peroxide (H2O2) in Chinese hamster lung fibroblast (V79-4) cells was investigated. QR evidenced a scavenging effect of 1,1-diphenyl-2-picrylhydrazyl (DPPH), superoxide, hydroxyl radicals and on intracellular ROS, and thus prevented lipid peroxidation. As a result, QR reduced H2O2-induced cell death and apoptosis in V79-4 cells. Moreover, H2O2 induced the cleavage of caspase-3, -9, and poly-ADP-ribose polymerase (PARP) and a reduction in Bcl-xL levels, whereas pretreatment with QR significantly inhibited caspase-3, -9, and PARP cleavage and the reduction in Bcl-xL levels, and ultimately ameliorated H2O2-induced apoptosis. Taken together, these results indicate that the treatment of V79-4 cells with QR can block H2O2-induced apoptosis via the regulation of Bcl-xL. QR may be exploited as a biopreservative in food applications or as a health supplement to alleviate oxidative stress.

10.1016/j.jff.2011.12.001

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Citations (34)

Hepatoprotective effects of dieckol-rich phlorotannins from Ecklonia cava, a brown seaweed, against ethanol induced liver damage in BALB/c mice

Food and Chemical Toxicology (2012)

Min‐Cheol Kang Ginnae Ahn Xiudong Yang Kil‐Nam Kim Sung‐Myung Kang

Malondialdehyde

Transaminase

Hepatotoxin

Aspartate transaminase

10.1016/j.fct.2012.03.078

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Citations (56)

[P8-356] Purification and characterization of a novel antioxidant protein from enzyme-assistant extracts of Styela clava

한국식품영양과학회 산업심포지움발표집 (2010)

Seok‐Chun Ko Sung‐Myung Kang W.A.J.P. Wijesinghe You‐Jin Jeon

Source

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The JNK/NFκB pathway is required to activate murine lymphocytes induced by a sulfated polysaccharide from Ecklonia cava

Biochimica et Biophysica Acta (BBA) - General Subjects (2012)

Ginnae Ahn So Jin Bing Sung‐Myung Kang WonWoo Lee Seung‐Hong Lee

IκBα

10.1016/j.bbagen.2012.12.008

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Citations (14)

Induction of Apoptosis by Citrus grandis Osbeck Peel (CGP) Extract in HL60 Cells

Journal of the Korean Society of Food Science and Nutrition (2009)

Jae-Seok Hyon Sung‐Myung Kang Areum-Daseul Kim Myung-Cheol Oh Chang-Kyung Oh

당유자 과피(GGP) 80% 에탄올 추출물을 4종의 암세포에(피부암, 대장암, 유방암 및 혈액암) 처리하여 증식 억제 활성을 측정한 결과, 혈액암 HL60 세포에서 높은 증식 억제 활성을 보였다. 이에 CGP 추출물이 HL60 세포에 대한 apoptosis 유도에 따른 세포 증식 억제 활성을 조사하였다. Apoptosis 유도의 첫 단계인 막 투과성을 측정한 결과, confocal image와 flow cytometry에서 CGP를 처리하였을 때 탈분극 현상에 따른 막 투과성이 증가하였고 세포내 핵을 hoechst 33342를 이용하여 염색하였을 때 apoptosis가 일어났을 때 나타나는 전형적인 형태의 apoptotic body가 농도 의존적으로 증가하는 것을 확인할 수 있었으며 flow cytometry를 통하여 세포 주기를 측정하였을 때 DNA-hypodiploid 형태의 sub-G1가 CGP 농도 의존적으로 증가하는 것을 확인할 수 있었다. Apoptosis 유도 기전을 western blot으로 측정한 결과를 보면, CGP 추출물을 혈액암 HL60 세포에 처리하였을 때 Bcl family의 anti-apoptotic Bcl-2 단백질의 감소와 pro-apoptotic Bax 단백질의 증가로 인하여 하위기전인 caspase-3가 활성화되었으며, 이 활성화로 인하여 apoptosis 유도에 직접적으로 관여하는 PARP 단백질을 활성화시키면서 apoptosis를 유도하였다. 따라서 당유자 과피는 항암과 관련되어진 기능성식품 및 소재 개발 원료로서 개발이 가능하리라고 사료된다.

HL60

10.3746/jkfn.2009.38.10.1317

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Citations (2)

[P8-288] High molecular weight fraction obtained from Sargassum coreanum enzymatic extract induces apoptosis for anticancer activity

한국식품영양과학회 산업심포지움발표집 (2007)

Sung‐Myung Kang Soo‐Jin Heo Kil‐Nam Kim Ki-Wan Lee You‐Jin Jeon

Sargassum

Fraction (chemistry)

Source

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