Objective To investigate the clinical significance of the expression of sphingolipids kinase-1 ( SPHK1 ) in neuroblastoma and its relationship with the clinical staging and prognosis of neuroblastoma. Methods The expression of SPHK1 in consecutive 142 paraffin-embedded sections of neuroblastoma from Jan. 1995 to Oct. 2009 was observed by immunohistological staining. The relationship between the expression of SPHK1 with the clinical staging and prognosis of neuroblastoma was analyzed. Results Low expression of SPHK1 was found in 69 cases of neuroblastoma and high expression in 73 cases. The expression of SPHK1 had a significant relationship with INSS staging (P ＜0.01 ) and survival (P ＜0.01 ) of patients with neuroblastoma. Conclusion There is a significant relationship between the expression of SPHK1 with the clinical staging and prognosis of neuroblastoma. SPHK1 can be used as a tumor marker of neuroblastoma.
Key words:
Sphingolipids kinase-1; Neuroblastoma; Expression
Ochratoxin A (OTA), a mycotoxin, is a potent nephrotoxin in humans and animals. Selenium (Se) is an essential micronutrient for humans and animals, and plays a key role in antioxidant defense. To date, little is known about the effect of Se on OTA-induced nephrotoxicity. In this study, the protective effects of selenomethionine against OTA-induced nephrotoxicity were investigated using the porcine kidney 15 (PK15) cells as a model. The results showed that OTA induced nephrotoxicity in a dose-dependent manner. Se at 0.5, 1, 2 and 4 μM had significant protective effects against OTA-induced nephrotoxicity. Furthermore, selenomethionine enhanced the activity and mRNA and protein expression of glutathione peroxidase 1 (GPx1), mRNA expression of GPx4, and mRNA expression of thioredoxin reductase 1 in the presence and absence of OTA. Among them, promoting effect of selenomethionine on GPx1 was maximal. Knock-down of GPx1 by using a GPx1-specific siRNA eliminated the protective effects of selenomethionine against OTA-induced nephrotoxicity. The results suggest that selenomethionine alleviates OTA-induced nephrotoxicity by improving selenoenzyme expression in PK15 cells. Therefore, selenomethionine supplementation may be an attractive strategy for protecting humans and animals from the risk of kidney damage induced by OTA.
Sam68 is a novel marker for aggressive neuroblastoma [Corrigendum] Zhao X, Li Z, He B, et al. Onco Targets Ther. 2013;6:1751–1760. In the author affiliation section on the first page, the affiliations were shown incorrectly. Read the original article
Background: Neuroblastoma (NB) is the most common solid extracranial tumor in children. However, the molecular mechanism and progression of NB is largely unknown, and unfortunately, the prognosis is poor. Src-associated in mitosis with a molecular weight of 68 kDa (Sam68) is associated with carcinogenesis and neurogenesis. The present study aimed to investigate the clinical and prognostic significance of Sam68 in NB. Methods: The expression of Sam68 in immortalized normal epithelial cells, NB cell lines, and in four cases of paired NB tissue and adjacent normal tissue from the same patient was examined using Western blotting, reverse transcription-polymerase chain reaction (PCR) and real-time reverse transcription-PCR. The proliferation of NB cells was determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Furthermore, Sam68 protein expression was analyzed in 90 NB cases characterized as clinicopathological using immunohistochemistry. Statistical analyses were applied to evaluate the diagnostic value and associations of Sam68 with clinical parameters. Results: Western blotting and reverse transcription-PCR showed that the expression level of Sam68 was markedly higher in NB cell lines than in the immortalized normal epithelial cells at both messenger RNA and protein levels. The MTT assay revealed that Sam68 expression supported proliferation of NB cells. Sam68 expression levels were significantly up-regulated in tumor tissues in comparison to the matched adjacent normal tissues from the same patient. Sam68 protein level was positively correlated with clinical stage ( P <0.001), tumor histology ( P <0.001), and distant metastasis ( P =0.029). Patients with higher Sam68 expression had shorter overall survival time, whereas those with lower tumor Sam68 expression had longer survival time. Conclusion: Our results suggest that Sam68 expression is associated with neuroblastoma progression and may represent a novel and valuable predictor for prognostic evaluation of neuroblastoma patients. Keywords: Sam68, biomarker, prognosis, neuroblastoma Corrigendum for this paper has been published
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