Ni‐Guang Xiao

Hunan Provincial People's Hospital

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Bing Zhang

University of Southern California

Han‐Chun Gao

Center for Excellence in Molecular Plant Sciences

Zhiping Xie

National Institute for Viral Disease Control and Prevention

Zhaojun Duan

National Institute for Viral Disease Control and Prevention

Li-Li Zhong

Hunan Provincial People's Hospital

Zhaojun Duan

National Institute for Viral Disease Control and Prevention

Xiao-Fang Ding

Hunan Normal University

Qionghua Zhou

Southeast University

Yunde Hou

National Institute for Viral Disease Control and Prevention

Saizhen Zeng

Hunan Provincial People's Hospital

Cooperative Institutions

Hunan Provincial People's Hospital

Hunan Normal University

Chinese Center For Disease Control and Prevention

Lanzhou University

National Institute for Viral Disease Control and Prevention

Chinese Academy of Medical Sciences & Peking Union Medical College

Huazhong University of Science and Technology

Shanghai Jiao Tong University

Chinese Academy of Sciences

Capital Medical University

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Novel Human Bocavirus in Children with Acute Respiratory Tract Infection

Emerging infectious diseases (2010)

Jingrong Song Yu Jin Zhiping Xie Han‐Chun Gao Ni‐Guang Xiao

Abstract Human bocavirus (HBoV) and HBoV2, two human bocavirus species, were found in 18 and 10 of 235 nasopharyngeal aspirates, respectively, from children hospitalized with acute respiratory tract infection. Our results suggest that, like HBoV, HBoV2 is distributed worldwide and may be associated with respiratory and enteric diseases.

Human bocavirus

Respiratory tract

10.3201/eid1602.090553

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Citations (51)

[Detection of respiratory pathogens and clinical features in 225 children with acute exacerbation of bronchial asthma].

PubMed (2020)

Tian Xie Li-Li Zhong Han Huang Xiaojuan Lin Ni‐Guang Xiao

To investigate the respiratory pathogens and clinical features in children with acute exacerbation of bronchial asthma.

Rhinovirus

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Citations (3)

[Viral etiology and risk factors for severe community-acquired pneumonia in children].

PubMed (2012)

Xiao-Fang Ding Bing Zhang Li-Li Zhong Ni‐Guang Xiao Qionghua Zhou

To study the virus spectrum of severe community-acquired pneumonia (CAP) and risk factors for the disease in children.Respiratory secretion specimens were collected from 1096 children hospitalized with CAP from June 2007 to November 2008, including 100 cases of severe CAP. Respiratory viruses were detected by PCR, nest-PCR or RT-PCR. Clinical data on the children were analyzed by univariate and multivariate logistic regression analysis for examining risk factors for severe CAP.Viral pathogens were isolated from 82 (82%) of the 100 cases with severe CAP. RSV was the most common (37%), followed by HBoV (25%) and HRV (18%). Mixed infection was noted in 32 cases (32%). The presence of underlying diseases (OR=6.623, P<0.01) and RSV infection (OR=1.672, P<0.05) were risk factors for severe CAP in children, while age was a protective factor (OR=0.475, P<0.01).RSV is the most frequent viral pathogen in children with severe CAP. The presence of underlying diseases and RSV infection may be risk factors for severe CAP, while age is a protective factor.

Etiology

Univariate analysis

Viral Pneumonia

Protective factor

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Citations (7)

[Establishment and clinical application of TapMan real-time RT-PCR method for detection of HHV-6].

PubMed (2013)

Qianqian Chen Bing Zhang Zhi-Ping Xie Jinsong Li Han‐Chun Gao

To establish a rapid, sensitive and specific real-time PCR method for detection of Human Herpesvirus-6 (HHV-6).According to the reference, a pair of primers and a probe were designed located in U65-66 gene and to set up the standards. We established a real-time RT-PCR method for detection of HHV-6, and to verify the specificity, sensitivity, reproducibility.The correlation coefficient was 0.999, E = 97.9%, the coefficient of variation values of Ct were 0.61% and 3.13% in real-time PCR assay for inter and intra assay, respectively. The results of all viruses were negative except of HHV-6 for the assay. The quantitative detection limit of the assay was 3 x 10(0) copies/microl.The real-time PCR assay is highly specific, sensitive and reproducible, which can be used to quatitative detecting clinical samples.

Coefficient of variation

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An analysis on viral prevalence of acute lower respiratory tract infection in children

Zhongguo yishi zazhi (2011)

Juan Wang Ni‐Guang Xiao Qionghua Zhou Rongfang Zhang Li-Li Zhong

Objective To investigate the viral prevalence of acute lower respiratory tract infection (ALRTI)in children. Methods Totally 1165 children with clinical diagnosis of ALRTI during the period from August 2007 to September 2008 were involved in our study. The nasopharyngeal aspirate specimen was collected from each patient. RT-PCRs were performed to detect common respiratory tract viruses including respiratory syncytial virus (RSV), rhinovirus (HRV), parainfluenza virus (PIV, type 1 -3 ), influzenza virus type A and B (IFA,IFB), and PCR was used to detect adenovirus (ADV). Results 783 patients were identified to have at least one kind of viral pathogens and the overall positive rate was 67.2%. The most common virus was RSV (27%), followed by HRV ( 17.4% ) and PIV3 ( 13. 9% ). The peak infection seasons were winter and autumn. The etiological spectrum of ALRTI varied in different age groups. Two or more viruses were identified in 284 out of 783 cases ( 36. 3% ). The mixed infection rate was high in infants under 1 year old (63.7%) while it decreased to 8. 5% in children older than 3 years of age. Conclusion RSV, HRV and PIV3 were the most predominant pathogens in children less than 1 year old. The peak infection seasons were winter and autumn. The infection rate and mixed infection rate in infants under 1 year old were highest. The most common style was RSV and HRV mixed infection. Key words: Respiratory tract infections/EP/VI; Viruses

Rhinovirus

Respiratory tract

Etiology

Viral infection

10.3760/cma.j.issn.1008-1372.2011.01.009

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Prevalence of human rhinovirus in children admitted to hospital with acute lower respiratory tract infections in Changsha, China

Journal of Medical Virology (2014)

Saizhen Zeng Ni‐Guang Xiao Zhiping Xie Guang‐Cheng Xie Lili Zhong

Abstract Human rhinovirus (HRV) is a causative agent of acute respiratory tract infections. This study analyzed the prevalence and clinical characteristics of three HRV groups (HRV‐A, ‐B, and ‐C) among 1,165 children aged 14 years or younger who were hospitalized with acute lower respiratory tract infection in China. PCR or reverse transcription‐PCR was performed to detect 14 respiratory viruses in nasopharyngeal aspirates collected from September 2007 to August 2008 in Changsha, China. HRV was detected in 202 (17.3%) of the 1,165 children; 25.3% of the HRV‐positive children were 13–36 months of age (χ 2 = 22.803, P = 0.000). HRV was detected year round and peaked between September and December. Fifty‐three percent of the HRV‐positive samples were also positive for other respiratory viruses; respiratory syncytial virus (RSV) was the most common secondary virus. Phylogenetic analysis using the VP4/VP2 region grouped the HRV‐positive strains as follows: 101 HRV‐A (50.0%), 21 HRV‐B (10.4%), and 80 HRV‐C (39.6%). HRV‐A infections occurred predominantly in spring and autumn, and the peak prevalence of HRV‐C was in early winter and late autumn. HRV‐B infections were less common in spring (χ 2 = 31.914, P = 0.000). No significant difference in clinical severity or presentation was found between patients with HRV single infection and HRV co‐detections. Furthermore, the clinical characterizations did not differ among the three HRV species. These results suggest that HRV‐C is an important viral agent along with HRV‐A and HRV‐B and that among hospitalized children with acute lower respiratory tract infection in China, the three HRV genotypes have similar clinical characteristics. J. Med. Virol. 86:1983–1989, 2014 . © 2014 Wiley Periodicals, Inc.

Rhinovirus

Respiratory tract

10.1002/jmv.23861

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Citations (20)

Prevalence of human metapneumovirus in children with acute lower respiratory infection in Changsha, China

Journal of Medical Virology (2013)

Ni‐Guang Xiao Bing Zhang Zhiping Xie Qionghua Zhou Rongfang Zhang

Human metapneumovirus (hMPV) causes acute respiratory infections in children. The prevalence and clinical characteristics of hMPV were determined in nasopharyngeal aspirates of children in Changsha, China. Reverse transcription-polymerase chain reaction (RT-PCR) or PCR was employed to screen for both hMPV and other common respiratory viruses in 1,165 nasopharyngeal aspirate specimens collected from children with lower respiratory tract infections from September 2007 to August 2008. All PCR products were sequenced, and demographic and clinical data were collected from all patients. Seventy-six of 1,165 (6.5%) specimens were positive for hMPV, of which 85.5% (65/76) occurred in the winter and spring seasons. The hMPV coinfection rate was 57.9% (44/76), and human bocavirus was the most common virus detected in conjunction with hMPV. Phylogenetic analysis revealed that 94.7% of the hMPV detected were of subgroup A2, 5.3% were subgroup B2, and none belonged to either the A1 or B1 subgroups. No significant differences were found in terms of the frequency of diagnosis and clinical signs between either the co- and mono-infection groups, or between patients with and without underlying diseases. It was concluded that hMPV is an important viral pathogen in pediatric patients with lower respiratory tract infections in Changsha. Only hMPV genotypes A2 and B2 were co-circulating in this locality; human bocavirus was the most common coinfecting virus, and coinfection did not affect disease severity.

Human bocavirus

Metapneumovirus

10.1002/jmv.23501

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Citations (14)

[Viral etiology in children with acute lower respiratory tract infections plus platelet disorders in Changsha, China: an analysis of 255 cases].

PubMed (2014)

Hua Liu Xiangling He Bing Zhang Ni‐Guang Xiao Yalan You

To investigate the viral etiology in hospitalized children with acute lower respiratory tract infections (ALRTI) plus platelet disorders.A total of 255 children with ALRTI plus platelet disorders and 442 children with ALRTI and normal platelets, all of whom were hospitalized between March 2010 and February 2011, were included in the study. Their nasopharyngeal aspirate samples were collected, and RT-PCR or PCR was performed to detect 14 viruses.Of 255 ALRTI patients with platelet disorders, thrombocytosis was found in 253 cases (99.2%) and thrombocytopenia in 2 cases (0.8%). Among ALRTI patients with platelet disorders, 173 (67.8%) were infected with at least one virus, with human rhinovirus as the most common one, followed by parainfluenza virus type 3 (PIV3) and respiratory syncytial virus (RSV). The detection rate of PIV3 in the abnormal platelet group was significantly higher than in the normal platelet group (P<0.05). In contrast, the detection rate of influenza virus B (IFVB) in the abonormal platelet group was significantly lower than in the normal platelet group (P<0.05). The age distribution showed significant difference between the abnormal and normal platelet groups (P<0.01). Platelet disorders were mainly found in children under one year of age (P<0.01).Thrombocytosis is often found in children with ALRTI caused by viruses, especially PIV3, but infection with IFVB seldom causes platelet disorders. Hospitalized children with ALRTI under one year tend to develop platelet disorders.

Thrombocytosis

Human bocavirus

Etiology

Rhinovirus

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Citations (1)

[Relationship between viral load of human bocavirus and clinical characteristics in children with acute lower respiratory tract infection].

PubMed (2017)

Xiao-Fang Ding Bing Zhang Li-Li Zhong Le‐Yun Xie Ni‐Guang Xiao

To investigate the prevalence of human bocavirus (HBoV) in children with acute lower respiratory tract infection and to explore the relationship between the viral load of HBoV and the clinical characteristics of acute lower respiratory tract infection in children.A total of 1 554 nasopharyngeal aspirates from children who were hospitalized due to acute lower respiratory tract infection between March 2011 and March 2014 were collected. Quantitative real-time PCR was used to detect 12 RNA and 2 DNA viruses, adenovirus (ADV) and HBoV, and to measure the viral load of HBoV in HBoV-positive children. A comprehensive analysis was performed with reference to clinical symptoms and indicators.In the 1 554 specimens, 1 212 (77.99%) were positive for viruses, and 275 (17.70%) were HBoV-positive. In HBoV-positive cases, 94.9% were aged <3 years, and there were more males than females. In the 275 HBoV-positive cases, 45 (16.36%) had single infection, and 230 (83.64%) had mixed infection. There was no significant difference in viral load between children with single infection and mixed infection (P>0.05). The patients with fever had a significantly higher viral load than those without fever (P<0.05). The children with wheezing had a significantly higher viral load than those without wheezing (P<0.05). There was no significant difference in viral load between children with mild, moderate, and severe acute lower respiratory tract infection (P>0.05).HBoV is one of the important pathogens of acute lower respiratory tract infection in children. Children with a higher viral load of HBoV are more likely to experience symptoms such as fever and wheezing. However, the severity of disease and mixed infection are not significantly related to viral load.

Human bocavirus

Respiratory tract

Viral infection

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Citations (4)

Prevalence and Clinical and Molecular Characterization of Human Metapneumovirus in Children With Acute Respiratory Infection in China

The Pediatric Infectious Disease Journal (2010)

Ni‐Guang Xiao Zhiping Xie Bing Zhang Xin‐hui Yuan Jingrong Song

Human metapneumovirus (HMPV), a newly discovered paramyxovirus, has been associated with acute respiratory tract infections (ARTIs). However, the prevalence and molecular characteristics of HMPV in China are still unclear.A total of 661 nasopharyngeal aspirates (NPA) specimens were collected from 661 children with ARTIs between December 2006 and November 2008. Specimens were screened for HMPV by reverse transcription-polymerase reaction. All positive amplification products were confirmed by sequencing.HMPV was detected in 45 patients (6.80%) of the 661 children. The HMPV-infected patients were from 29 days to 9 years of age. A high incidence of HMPV infection (84.4%) was observed during the winter-spring season. Of the 45 HMPV-positive patients, 25 (55.6%) were co-infected with other respiratory viruses, and respiratory syncytial virus (RSV) was the most common additional respiratory virus. The most common clinical diagnosis was bronchopneumonia (57.8%) and cough (88.9%) was the most common clinical symptom. Phylogenetic analysis of the F gene revealed that 80% of the HMPV detected were A2, 2.2% were A1, and 17.8% were B1. Statistical analyses showed that sex, ages, seasons, and severity of the disease did not correlate with HMPV genotype (P = 0.986, 0.347, 0.660, 0.252), but viral coinfection with HMPV increased hospitalization rates (P = 0.005).HMPV was frequently detected in the pediatric patients with ARTI in China. RSV was the most common coinfection virus and coinfection increased hospitalization rates. All HMPV subgroups except B2 cocirculated and there was no association found between HMPV genotypes and severity of disease.

Metapneumovirus

10.1097/inf.0b013e3181b56009

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Citations (27)