Naoko Takahashi

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Trichothecene biosynthesis in different fungal genera: resistance mechanisms, pathway enzymes, and their product applications.

JSM Mycotoxins (2020)

Naoko Takahashi Kosuke Matsui Tamotsu Suzuki Kazuki Sadamatsu Hayato Azuhata

トリコテセン系カビ毒は,Fusarium,Trichothecium,Trichoderma,Myrothecium属等の糸状菌によって生産され,食や環境を通じて人々の健康を脅かす.これらのトリコテセンは,これまでその構造からA型(type A)からD型(type D)に分類されてきた.しかし,生合成経路の見地から見れば,trichodieneから連続的酸化を経て,isotrichodiolの環化によって生じるd-typeトリコテセンとisotrichotriolの環化によって生じるt-typeトリコテセンに分類する方が合理的とも言える.我々は,トリコテセン生産菌の遺伝子組換え株を用い,天然型・非天然型のトリコテセンを生産・精製・同定してきた.本稿では,これまでの研究で得られた知見について,ここに報告する.

10.2520/myco.70-2-3

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Citations (4)

[Transient ischemic dilatation of the left ventricle observed on dipyridamole-stressed thallium-201 scintigraphy].

PubMed (1993)

Takuji Toyama B Caner Nobuharu Tamaki Kazuki Yamamoto Tetsuo Nakashima

To assess clinical significance of transient ischemic dilatation of the left ventricle (TID) on dipyridamole-stressed 201Tl myocardial scintigraphy, 110 patients suspected with coronary artery disease, including 13 with normal coronary, 48 with single vessel disease, 30 with 2 vessel disease, and 19 with 3 vessel disease were analyzed. TID was visually and quantitatively assessed calculating the ratio of the area within the left ventricular region on the initial and delayed image (TID ratio). TID was observed in 3 of 1 vessel disease (6%), 5 of 2 vessel disease (17%), and 8 of 3 vessel disease (42%). None of normal coronary artery showed TID. In patients with multivessel disease (MVD), TID was observed more frequently (27%) than in patients with 1 vessel disease (6%) (p < 0.025). Furthermore, the TID ratio tended to be higher (1.15 +/- 0.11) than those with single vessel disease (1.03 +/- 0.03). To identify patients with MVD, TID had a sensitivity of 27% and a specificity of 95%. ECG changes were observed more often in patients with TID than those without TID. Five patients showing TID underwent coronary artery bypass graft surgery, TID disappeared postoperatively in each patient. In conclusion, TID on dipyridamole 201Tl scan seems to be a specific although not sensitive marker for detecting MVD.

Dipyridamole

Thallium

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Citations (6)

デオキシニバレノール生合成経路の少量中間体7-hydroxyisotrichoderminと8-hydroxyisotrichoderminのトリコテセン高感受性酵母を用いた毒性検証

JSM Mycotoxins (2015)

Kamata Kentaro Akira Tanaka Kazuyuki Maeda Takushima Ryo Hiroki Sato

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Haloparvum alkalitolerans sp. nov., alkali-tolerant haloarchaeon isolated from commercial salt

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY (2016)

Yusuke Kondo Hiroaki Minegishi Akinobu Echigo Yasuhiro Shimane Masahiro Kamekura

A Gram-stain-negative, rod-pleomorphic, aerobic, halophilic archaeon, strain MK62-1T, was isolated from commercial salt made from seawater in the Philippines. Strain MK62-1T was able to grow at 2.1-4.7 M NaCl (with optimum at 2.1-2.6 M NaCl), pH 6.5-9.5 (optimum, pH 7.0-7.5) and 20-55 °C (optimum, 45-50 °C). Based on the orthologous 16S rRNA gene sequence, the closest relative was Haloparvum sedimenti JCM 30891T with 99.2 % similarity. The RNA polymerase subunit B' gene sequence also showed the highest similarity (97.4 %) to that of Haloparvum sedimenti DYS4T. The DNA G+C content of MK62-1T was 70.1 mol%, while that of Haloparvum sedimenti JCM 30891T was 69.5 mol% by the HPLC method. The levels of DNA-DNA relatedness between MK62-1T and Haloparvum sedimenti JCM 30891T were 60.6 and 60.8 % (reciprocally). The major polar lipids of the isolate were C20C20 archaeol derivatives of phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester and phosphatidylglycerol sulfate. Based on the phenotypic and phylogenetic analyses, it is proposed that the isolate represents species within the genus Haloparvum, for which the name Haloparvum alkalitolerans sp. nov. is proposed. The type strain is MK62-1T (=JCM 30442T =KCTC 4214T).

Phosphatidylglycerol

Haloarchaea

Strain (injury)

10.1099/ijsem.0.001513

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Citations (12)

Geranylgeranylacetone protects cultured guinea pig gastric mucosal cells from indomethacin.

Digestive Diseases and Sciences (2000)

Wataru Tomisato Naoko Takahashi Chiho Komoto Kazuhito Rokutan Tomofusa Tsuchiya

Cytoprotection

Antiulcer drug

10.1023/a:1005597902470

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Citations (40)

Cloning and characterization of genes specifically expressed during infection stages in the rice blast fungus

FEMS Microbiology Letters (2003)

Shinpei Banno Makoto Kimura Takeshi Tokai Shin Kasahara Arisa Higa-Nishiyama

We isolated promoters of 12 genes from the rice blast fungus based on the sequences of randomly selected expressed sequence tags (ESTs) (appressorium formation stage cDNA library of Magnaporthe available from GenBank). These promoters (and the 5' coding regions if any) were fused in frame with egfp, and their expression patterns were examined under the epifluorescence microscope. Among them, two turned out to be specifically active in structures necessary for infection, viz. a promoter of adenylate cyclase interacting protein 1-like gene expressed in conidia, germ tubes, and appressoria, and a promoter of putative membrane-associated or secreted protein gene specifically expressed in appressoria. Although targeted knockout mutants of either gene failed to show detectable phenotypic alterations under laboratory conditions, these ESTs should be useful for identification of genes expressed during infection stages.

Appressorium

Magnaporthe

Cloning (programming)

10.1016/s0378-1097(03)00307-0

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Citations (21)

Effects of oral intake, symptom severity, and sex difference on cancer patient prognosis

Supportive Care in Cancer (2025)

Tetsuya Ito Emi Tomizawa Yuki Yano Dai Akiyama Haruko Konishi

Performance status

Pain medicine

Medical record

10.1007/s00520-024-09124-8

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REG1A expression is an independent factor predictive of poor prognosis in patients with non-small cell lung cancer

Lung Cancer (2007)

Yoshihiro Minamiya Hideki Kawai Hajime Saito Manabu Ito Yukiko Hosono

10.1016/j.lungcan.2007.09.012

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Citations (23)

Inhibition of the 53BP2S‐mediated apoptosis by nuclear factor κB and Bcl‐2 family proteins

Genes to Cells (2005)

Naoko Takahashi Shinya Kobayashi Shinichi Kajino Kenichi Imai Keisuke Tomoda

The p53 binding protein 2 (53BP2) has been identified independently as the interacting protein to p53, Bcl‐2, and p65 subunit of nuclear factor κB (NF‐κB). It was demonstrated that over‐expression of 53BP2 (renamed as 53BP2S) induces apoptotic cell death. In this study we explored the effect of NF‐κB activation elicited by a physiological NF‐κB inducer, interleukin‐1β (IL‐1β), and anti‐apoptotic Bcl‐2 family proteins on the 53BP2S‐mediated apoptosis. We found that both NF‐κB activation and Bcl‐2 family proteins could prevent the 53BP2S‐mediated depression of mitochondrial transmembrane potential, activation of caspase‐9, cleavage of poly ADP ribose polymerase (PARP), and cell death. These observations suggested that 53BP2S/Bbp and its directly or indirectly interacting proteins might play crucial roles in the regulation of apoptosis and contribute to carcinogenesis. It is also suggested that 53BP2S/Bbp induces apoptosis through the mitochondrial death pathway presumably by counteracting the actions of anti‐apoptotic Bcl‐2 family proteins. The regulatory network of the 53BP2S‐mediated apoptosis cascade including its interacting proteins is discussed.

Bcl-2 Family

10.1111/j.1365-2443.2005.00878.x

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Citations (20)

Cloning of genes encoding animal cell growth factors and the expression of these genes in transgenic plants

Jūigaku kenkyū/Japanese journal of veterinary research (2001)

Naoko Takahashi

Cloning (programming)

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Citations (0)