Qiong Yan

University of Science and Technology Beijing

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Jian‐Yong Wu

Hong Kong Polytechnic University

Zongding Hu

Tianjin University

Janet Ng

Kaiser Permanente

Ming Shi

Sun Yat-sen University

Rui Liu

Sichuan University

Ren Xiang Tan

Nanjing University

Changhe Zhang

Chongqing University of Technology

Wai-Keung Cheuk

Xiao Wei Du

University of Science and Technology Beijing

Xiao Jie Geng

Hefei University of Technology

Cooperative Institutions

Hong Kong Polytechnic University

Nanjing University of Chinese Medicine

Kaiser Permanente

Kaiser Permanente Washington Health Research Institute

Western Infirmary

Guizhou University

State Key Laboratory of Food Science and Technology

Nanchang University

Jiangsu Province Hospital

First Affiliated Hospital Zhejiang University

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Modeling of tanshinone synthesis and phase distribution under the combined effect of elicitation and in situ adsorption in Salvia miltiorrhiza hairy root cultures

Biotechnology Letters (2010)

Qiong Yan Jian‐Yong Wu Rui Liu

Salvia miltiorrhiza

10.1007/s10529-010-0484-5

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Citations (16)

Research on Influence of Span on Surrounding Rock Quality of Tunnels

Advanced materials research (2014)

Xiao Jie Geng Xiao Wei Du Qiong Yan

The influence of tunnel’s span on rock mass quality is getting more and more recognition along with increasing large span tunnels’ construction. As national standard of China, BQ qualification is generally applied. Based on the measured data and numerical experiment, the relationship between surrounding rock displacement and quality evaluation is studied, and span effect correction factor is established. The modified rock quality index can provide the basis of guiding the design and construction of tunnels.

10.4028/www.scientific.net/amr.919-921.869

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Citations (1)

Efficient production and recovery of diterpenoid tanshinones in Salvia miltiorrhiza hairy root cultures with in situ adsorption, elicitation and semi-continuous operation

Journal of Biotechnology (2005)

Qiong Yan Zongding Hu Ren Xiang Tan Jian‐Yong Wu

Salvia miltiorrhiza

Hairy Root Culture

Fibrous root system

10.1016/j.jbiotec.2005.04.020

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Citations (94)

Enhancement of Tanshinone Production inSalvia miltiorrhizaHairy Root Culture by Ag⁺Elicitation and Nutrient Feeding

Planta Medica (2004)

Changhe Zhang Qiong Yan Wai-Keung Cheuk Jian‐Yong Wu

Ag+ was employed as an abiotic elicitor to stimulate the secondary metabolite production in hairy root culture of Salvia miltiorrhiza, a valuable herbal plant. The addition of Ag2S2O3 at 15 - 40 μM to the culture between 12 and 22 days post inoculation resulted in more than 2-fold increase in the yields of the three diterpenoid tanshinones, tanshinone I, tanshinone IIA and cryptotanshinone. This stimulating effect was dependent on the Ag+ dose, the day of Ag+ addition to the culture and the tanshinone species, while the total yield of the three tanshinones (TT) was mainly dependent on the Ag+ dose, with the highest being attained at 30 μM. The Ag+ treatment also caused a dose-dependent inhibition of hairy root growth. Sucrose feeding or medium renewal before the addition of Ag+ to the culture effectively prevented the growth inhibition, and significantly increased the biomass concentration and volumetric tanshinone yield. With combined medium renewal and Ag+ treatment, in particular, the TT yield was increased 6.6-fold relative to that of the control (55.7 mg/L versus 7.3 mg/L). The elicitor function of Ag+ in the hairy root culture was validated by its ability to induce the characteristic elicitor responses of plants, the increase in cross-cell membrane ion fluxes and the production of reactive oxygen species.

Salvia miltiorrhiza

Hairy Root Culture

Secondary metabolite

Angelica sinensis

10.1055/s-2004-815492

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Citations (97)

Analysis of Influence of Flat-flat Technological Parameters on the Creasing Process of Corrugated Board

Journal of Wuhan University of Technology-Mater Sci Ed (2008)

Qiong Yan

Parametric design

Linearity

Basis (linear algebra)

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Elicitor-induced rosmarinic acid accumulation and secondary metabolism enzyme activities in Salvia miltiorrhiza hairy roots

Plant Science (2006)

Qiong Yan Ming Shi Janet Ng Jian‐Yong Wu

Rosmarinic acid

Salvia miltiorrhiza

Phenylalanine ammonia-lyase

Tyrosine aminotransferase

Secondary metabolism

Hairy Root Culture

10.1016/j.plantsci.2005.12.004

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Citations (191)

Influence of biotic and abiotic elicitors on production of tanshinones in Salvia miltiorrhiza hairy root culture

Zhongcaoyao (2006)

Qiong Yan Zongding Hu

Objective To compare influence of several biotic elicitors(yeast extract,(oligogalactu-)ronides,and fungal elicitor) and abiotic elicitors(Ag~+,Co~(2+),and α-amino isobutyric acid) on tanshinone production in the hairy root culture of Salvia miltiorrhiza.Methods The contents of three tanshinones were(analyzed) by high performance liquid chromatography.Results Biotic elicitors increased effectively the yield of tanshinones and there were difference among different elicitors' effects.The highest content of tanshinones was achieved by fungal elicitor at 100 μg/mL,which was(0.42 mg/g),4.7-fold of the control.The elicitation effects of abiotic elicitors were relatively lower than those of biotic elicitors,with Co~(2+) at 50 μmol/L giving the highest content of tanshinone(1.75 mg/g,4.1-fold of the control) among abiotic elicitors.Biotic and abiotic elicitors showed the selectivity of elicitation.All biotic elicitors notably increased the crypotanshinone content and all abiotic elicitors selectively enhanced the tanshinone I content.And in most cases,the elicitors added did not cause any obviously inhibitory effect on the hairy root growth. Conclusion Adding different biotic and abiotic elicitors may selectively promote tanshinone accumulation and not affect the hairy root growth in the hairy root cultures of S.miltiorrhiza.

Salvia miltiorrhiza

Plant Physiology

Hairy Root Culture

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Citations (5)