Hong Yao

China Pharmaceutical University

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Shengtao Xu

Hubei University of Technology

Pijun Zhou

China Pharmaceutical University

Chen He

St. Jude Children's Research Hospital

Yiping Duan

China Pharmaceutical University

Wenyi Zhong

China Pharmaceutical University

Baixue Zhang

China Pharmaceutical University

Xiaoming Wang

Guizhou University

Juntao Sun

Xuchang University

Hongxin Wang

Jiangnan University

Fei Xiong

Southeast University

Cooperative Institutions

Jiangnan University

State Key Laboratory of Food Science and Technology

China Pharmaceutical University

Chinese Academy of Sciences

Sun Yat-sen University

Soochow University

Sichuan University

Nanjing Medical University

First Affiliated Hospital of Soochow University

Nanjing University

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The immunogenicity of an engineered chimeric C-terminal peptide of beta human chorionic gonadotropin

Zhonghua weishengwuxue he mianyixue zazhi (2000)

Jian Wu Jianhua Yan Hong Yao Rong Zhang

Objective To enhance the immunogenicity of the COOH terminal peptide of beta human chorionic gonadotropin(βhCG CTP) using molecular engineering. Methods Based on the analysis of protein epitope and principles of immune recognition, a chimerical peptide comprised of the beta 8 and beta 9 epitopes of hCG and a “promiscuous ” T cell epitope from the fusion protein of measles virus (MVF) was devised, synthesized and then purified. Rabbits were immunized with the chimerical peptide. The COOH terminal peptide of beta human chorionic gonadotropin coupled to diphtheria toxoid provided by WHO was served as control. Antibody titers were determined by enzyme linked immunosorbent assay (ELISA). Results The synthesized chimerical peptide elicited a much higher antibody response against intact native human chorionic gonadotropin than the control. Conclusion The synthesized chimerical peptide is more immunogenic and there fore it is possible to enhance the immunogenicity of beta hCG CTP via molecular engineering, which may be a promising approach for the making of an efficient vaccine for human fertility control.

Human chorionic gonadotropin

Peptide vaccine

Antigenicity

Toxoid

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Purification and Characterization of Lipase Produced by Aspergillus oryzae CJLU-31 Isolated from Waste Cooking Oily Soil

American Journal of Food Technology (2012)

Jing Zhou Weiwen Chen Zheng-Bao Jia Guangrong Huang Hong Yao

Aspergillus oryzae

10.3923/ajft.2012.596.608

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Citations (15)

Cloning and expression of a thermostable β-1,3-1,4-glucanase from Bacillus amyloliquefaciens ATCC 23350

Annals of Microbiology (2011)

Juntao Sun Hongxin Wang Wenping Lv Chaoyang Ma Zaixiang Lou

Bacillus amyloliquefaciens

Glucanase

Cloning (programming)

Bacillaceae

10.1007/s13213-011-0366-7

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Citations (12)

Cloning and Sequence Analysis of β-1,3-1,4-Glucanase Gene

Journal of Food Science and Biotechnology (2011)

Hong Yao

Based on the sequence alignment of Bacillus amyloliquefaciens,two pairs of primers were designed and synthesized in this study.Using the total DNA of Bacillus amyloliquefaciens as template,the gene of β-1,3-1,4-glucanase(bgl) was amplified by PCR,which has about 750 bp in length.The gene was cloned into pTG19-T Easy vector,and the recombinant plasmid was identified by PCR,restriction enzyme analysis and sequencing.The homology analysis revealed that the nucleotide sequences of the cloned β-1,3-1,4-glucanase gene similar to the B.amyloliquefacines(M15674),B.subtilis(D00518) and B.licheniformis(AY365256)were 99%,95% and 94%,respectively.

Bacillus amyloliquefaciens

Glucanase

Cloning (programming)

Bacillus licheniformis

Cloning vector

Homology

Restriction site

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Inhibitory effect of magnetic Fe<sub>3</sub>O<sub>4</sub> nanoparticles coloaded with homoharringtonine on human leukemia cells in vivo and in vitro

International Journal of Nanomedicine (2016)

Suning Chen Mei‐Yu Chen Fei Xiong Liang Ma Hong Yao

Homoharringtonine (HHT), a natural cephalotaxine alkaloid, has been used in the People's Republic of China for treatment of leukemia for >3 decades. Here, we employed magnetic Fe3O4 nanoparticles (MNP-Fe3O4) to improve the therapeutic effect of HHT and investigated its biological effects. Within a certain range of concentrations, the HHT-MNP-Fe3O4 showed a more enhanced inhibitory effect on the selected myeloid leukemia cell lines than HHT alone. Compared with HHT, HHT-MNP-Fe3O4 could induce more extensive apoptosis in leukemia cells, which also showed more pronounced cell arrests at G0/G1 phase. HHT-MNP-Fe3O4 enhanced antitumor activity by downregulating myeloid cell leukemia-1, which could inhibit the activation of caspase-3 and poly-ADP-ribose polymerase. In vivo experiments using tumor-bearing animal models showed that the mean tumor volume with HHT-MNP-Fe3O4 was significantly smaller than that with HHT alone (193±26 mm3 versus 457±100 mm3, P<0.05), while the mean weight was 0.67±0.03 g versus 1.42±0.56 g (P<0.05). Immunohistochemical study showed fewer myeloid cell leukemia-1-stained cells in mice treated with HHT-MNP-Fe3O4 than with the controls. These findings provide a more efficient delivery system for HHT in the treatment of hematological malignancy.

Homoharringtonine

10.2147/ijn.s105543

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Citations (17)

Design, synthesis, and biological evaluation of novel HPK1 inhibitors possessing 3-cyano-quinoline moiety

Bioorganic Chemistry (2024)

Long Chen Baixue Zhang Pijun Zhou Yiping Duan Chen He

Moiety

Quinoline

10.1016/j.bioorg.2024.107814

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Citations (0)