Saori Itoh

Gifu Pharmaceutical University

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Co-Authors

Hiroshi Mori

The Graduate University for Advanced Studies, SOKENDAI

Nobuko Ohishi

Furukawa Electric (Japan)

Shin‐ichiro Yokoyama

University of Palermo

Kunio Yagi

Institute of Applied Biochemistry

Tohru Suzuki

National Institute of Animal Health

Kenshiro Shiraishi

Teikyo University

Kuni Ohtomo

International University of Health and Welfare

Akihiro Haga

Tokushima University

Keiichi Nakagawa

The University of Tokyo

Shuichi Shiraishi

Gifu Pharmaceutical University

Cooperative Institutions

High Energy Accelerator Research Organization

The University of Tokyo

European Organization for Nuclear Research

Tohoku University

A. Alikhanyan National Laboratory

Kyoto University

Nagoya University

The Graduate University for Advanced Studies, SOKENDAI

Osaka University

SLAC National Accelerator Laboratory

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New Rapid Enzyme-Linked Immunosorbent Assay to Detect Antibodies against Bacterial Surface Antigens Using Filtration Plates.

Biological and Pharmaceutical Bulletin (2002)

Saori Itoh Masako Kariya Keiji Nagano Shin‐ichiro Yokoyama Toshio Fukao

An easy and rapid ELISA system, Filtration ELISA, to detect antibodies against bacterial cell surface antigens was developed using a 96-well filtration plate fitted with a 0.22microm membrane (MultiScreen-GV, Millipore). Bacterial whole cells were used as antigens without fixing the cells with formalin etc. The whole cell antigens were washed by vacuum filtration through a filter and resuspended in washing buffer. Assay reactions could be done in the wells without losing the solution. The technique was established using antisera of mice immunized with Escherichia coli, and then evaluated by assaying antibodies to Shiga-toxin producing E. coli O157:H7 (STEC), Staphylococcus aureus and Lactobacillus acidophilus in fecal extracts of 157 children who had eaten school lunches contaminated with STEC in comparison with 25 age-matched control children. The lunch group showed significantly higher IgA antibody titers against STEC than the control group (p<0.0005), but not against L. acidphilus. The results indicate that Filtration ELISA is a quantitative and specific technique for measuring antibodies against antigens on the surface of bacteria without extracting antigens from the bacteria. This technique is widely applicable to the assay of antibodies in various samples including serum and fecal extract against various kinds of bacteria.

Filtration (mathematics)

Antibody titer

10.1248/bpb.25.986

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Citations (30)

Nucleotide Sequence Analysis of Shiga(-like) Toxins from an Enterohemorrhagic Escherichia coli Isolated from the Gifu, Japan, Outbreak.

Journal of Clinical Biochemistry and Nutrition (2000)

Shin‐ichiro Yokoyama Tohru Suzuki Keiichi Kawai Nobuko Ohishi Kunio Yagi

Two Shiga toxin (Stx) genes (stx-1 and stx-2) were cloned and sequenced from a strain of enterohemorrhagic Escherichia coli, serotype O157:H7, a clinical isolate from the Gifu City, Japan, outbreak in 1996 (GPU96MM). The 1, 257 by of stx-1 and 1, 321 by of stx-2 genes were analyzed, and each gene contained two open reading frames (ORFs), 948 or 960 by of A and 270 by of B subunits, respectively. Although the nucleotide sequences of stx-1 ORFs of this isolate had several nucleotide alterations, the deduced amino acid sequence was identical to that of stx-1 from E. coli (O157:H7) isolated from Canada and stx from Shigella dysenteriae type 1. The nucleotide sequences of the stx-2 ORFs of this strain were identical to those of stx-2 from two E. coli (O157:H7) strains, KNIH317 isolated from Korea and 933.

ORFS

Strain (injury)

10.3164/jcbn.28.53

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Citations (2)

Construction of deletion mutants of Shiga(-like) toxin genes(stx-1 and/or stx-2) on enterohemorrhagic Escherichia coli(O157 : H7).

岐阜藥科大學紀要 = The annual proceedings of Gifu College of Pharmacy (2002)

Yokoyama Shin-ichiro Tohru Suzuki Shuichi Shiraishi Nobuko Ohishi Kunio Yagi

Shiga-like toxin

STX2

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Citations (0)

GLD Detector Outline Document: Version 1.2

Koh Abe M. Thomson N. Graf Yoshio Ishizawa Xinmin Zhang

The GLD is a detector for the experiment at the International Linear Collider (ILC). It consists of a large calorimeter and a gaseous central tracker placed in a moderate magnetic field, both electro-magnetic and hadron calorimeters being placed inside the magnetic coil to have enough hermeticity and good jet energy resolution. The outline of the GLD is presented.

Calorimeter (particle physics)

International Linear Collider

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Sexual hybridization of Mating-incompetent Sake Yeasts by Isolation of Mating-competent Mutants

JOURNAL OF THE BREWING SOCIETY OF JAPAN (1999)

Yoko Okuno Ken-Ichi YOKOHARA Saori Itoh Haruhiko Sugino Mamoru TAKASE

Yeast strains used for sake brewing are, in general, difficult to sporulate and incompetent at mating with others.We isolated eight mating-competent mutants from strain Kyokai no.7 and three other strains for ginjo-shu making with the aid of pNN63 and pNN64 plasmids.These plasmids were collstructed by NAKAZAWA et al., (J.Ferment.Bioeng., 78: 6-11, 1994) for the detection of the mating competency of the host cells.With these mating-competent mutants and three haploid strains used in genetic studies, we constructed 21 putative hybrids by crossing, and found that 8 of the 21 isolates are putative triploids and the remaining 13 are tetraploids.To see their hybridity, the putative hybrids were examined for their electrophoretic karyotypes and sporulation abilities.We found that 17 of the 21 isolates are hybrids.The sake brewing characteristics of the hybrid clones were examined on a small scale artificial sake mash, then by 1 kg pilot brewing using five selected isolates.The results suggested that some of the hybrids are superior for sake brewing with regard to their taste and aroma, but none were superior to Kvokai no.7 and no.901 with regard to mash fermentability.

Strain (injury)

10.6013/jbrewsocjapan1988.94.575

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Nucleotide sequence analysis of Shiga(-like) toxins from an exterohemorrhagic Escherichia coli isolated from the gifu, Japan, outbreak.

The annual proceedings of Gifu College of Pharmacy (2002)

Shin‐ichiro Yokoyama Tohru Suzuki Keiichi Kawai Nobuko Ohishi Kunio Yagi

Sequence (biology)

Source

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Quality assurance of volumetric modulated arc therapy using Elekta Synergy

Acta Oncologica (2009)

Akihiro Haga Keiichi Nakagawa Kenshiro Shiraishi Saori Itoh Atsuro Terahara

10.1080/02841860903081905

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Citations (3)

First report on prostate displacements immediately before and after treatment relative to the position during VMAT delivery

Acta Oncologica (2009)

Keiichi Nakagawa Kenshiro Shiraishi Satoshi Kida Akihiro Haga Kentaro Yamamoto

Position (finance)

10.1080/02841860903101190

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Citations (0)

Construction of Deletion Mutants of Shiga (-Like) Toxin Genes (stx-1 and/or stx-2) on Enterohemorrhagic Escherichia coli (O157: H7).

Journal of Clinical Biochemistry and Nutrition (2001)

Shin‐ichiro Yokoyama Tohru Suzuki Shuichi Shiraishi Nobuko Ohishi Kunio Yagi

We constructed isogenic Shiga (-like) toxin (Stx-1 and/or Stx-2) gene-deletion mutants of enterohemorrhagic Escherichia coli (EHEC) GPU96MM (O157:H7). A vector with temperature-sensitive replication origin was used for the construction. The parts of stx-1 and stx-2 on the GPU96MM genome were replaced with kanamycin and chloramphenicol resistance genes, respectively. The mutants deficient in stx-1, stx-2, and both of them were named GPU993, GPU994, and GPU995, respectively. Each mutation was confirmed by the polymerase chain reaction, enzyme-linked immunosorbent assay using antibodies to B subunits of Stx-1 and Stx-2, and the cytotoxic activity of the bacterial culture supernatants toward HeLa cells was detected for GPU96MM and the mutants except for GPU995. These results indicate that GPU993 and GPU994 lack productivity for the respective toxins and GPU995, for both of them.

Kanamycin

10.3164/jcbn.30.33

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Citations (10)