Huizi Ouyang

First Teaching Hospital of Tianjin University of Traditional Chinese Medicine

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Jun He

Tianjin University of Traditional Chinese Medicine

Yanxu Chang

Tianjin University of Traditional Chinese Medicine

Guangzhe Yao

Tianjin University of Traditional Chinese Medicine

Xuhua Huang

Tianjin University of Traditional Chinese Medicine

Yameng Zhu

Nanjing Tech University

Wenjuan Ma

Nanjing Forestry University

Zhenguo Lv

First Teaching Hospital of Tianjin University of Traditional Chinese Medicine

Jiayuan Shen

Tianjin University of Traditional Chinese Medicine

Xiunan Cao

Tianjin University of Traditional Chinese Medicine

Yuqi Fan

Wenzhou Medical University

Cooperative Institutions

Tianjin University of Traditional Chinese Medicine

Chinese Academy of Sciences

First Teaching Hospital of Tianjin University of Traditional Chinese Medicine

Chinese Academy of Medical Sciences & Peking Union Medical College

Tianjin haihe hospital

Sun Yat-sen University

Fudan University

Tianjin University

Wenzhou Medical University

Shanghai Jiao Tong University

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SET7/9 in complex with inhibitor (R)-(3-(3-cyanophenyl)-1-oxo-1-(pyrrolidin-1-yl)propan-2-yl)-1,2,3,4-tetrahydroisoquinoline-6- sulfonamide and S-adenosylmethionine

J.R. Walker Huizi Ouyang A. Dong Paul V. Fish Angus Cook

Sulfonamide

Tetrahydroisoquinoline

10.2210/pdb4e47/pdb

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Development of a HPLC-MS/MS Method to Determine the 13 Elements of Semen Cuscutae and Application to a Pharmacokinetic Study in Rats

Evidence-based Complementary and Alternative Medicine (2019)

Jiayuan Shen Jia Qi Xuhua Huang Guangzhe Yao Wenjuan Ma

This study developed a method for simultaneous determination of 13 elements of Semen Cuscutae (quercitrin, quercetin, hyperoside, caffeic acid, chlorogenic acid, luteolin, apigenin, kaempferol, isoquercitrin, cryptochlorogenic acid, isorhamnetin-3-O-glucoside, astragalin, and rutin) in rat plasma using high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) in the negative MRM mode. The analytes were analyzed with CORTECS®C18 column (4.6 × 150 mm, 2.7 μm) with mobile phases consisting of 0.1% formic acid in water (A) and acetonitrile (B). The intra- and interday precision of the target compounds were expressed as relative standard deviation (RSD) in the range of 0.5%-10.4%, and the accuracy of the target compounds was expressed as relative error (RE) not exceeding ±14.5% for all analytes. In the meantime, the extraction recovery of the target compounds in plasma samples ranged from 87.4% to 106.2% and matrix effect from 81.0% to 115.5%. The established method was successfully accomplished for the pharmacokinetic study of the analytes in rat plasma samples following oral administration of Semen Cuscutae extract, and the pharmacokinetic parameters of seven compounds were obtained.

Hyperoside

Chlorogenic Acid

Astragalin

Isorhamnetin

Quercitrin

10.1155/2019/6518528

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Citations (16)

Comparative analysis of chemical components in fruits of Chebulae Fructus and its pulp based on chromatographic technology coupled with multivariate chemometric methods

Journal of Pharmaceutical and Biomedical Analysis (2023)

Guangzhe Yao Xinxin Miao Minglei Ge Mengxuan Wu Yu Bai

Chemometrics

10.1016/j.jpba.2023.115735

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Study on excretion of stilbene glycoside (THSG) and its β-cyclodextrin inclusion

China Journal of Chinese Materia Medica (2010)

Xiaoliang Ren Huizi Ouyang Guifang Wang Min Zhao Ai‐Di Qi

The excretion characteristics of stilbene glycoside (THSG) and its beta-cyclodextrin inclusion in bile, urine and feces after oral administration to rats were studied. Bile for 24 h, urine and feces for 72 h were collected. The content of THSG was determined by HPLC-UV. The established HPLC-UV method was available for the analysis of THSG in excreta and corresponded to the requirement of biological sample analysis. After given THSG and its beta-cyclodextrin inclusion, the amount of prototype THSG in feces were 3.27% and 0.61%, meanwhile THSG in bile were 0.20% and 0.18%, respectively. Only a little THSG was found in urine. The result showed that beta-cyclodextrin inclusion reduced the fecal excretion of THSG. However, the characteristic of urinary and biliary excretion wasn't changed.

Inclusion compound

10.4268/cjcmm20101927

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Comparison of the chemical profile differences of Aster tataricus between raw and processed products by metabolomics coupled with chemometrics methods

Journal of Separation Science (2021)

Songrui Wang Zixiang Xue Xuhua Huang Wenjuan Ma Dongyue Yang

Aster tataricus, a traditional Chinese herb, has been used to treat cough and asthma for many years. Its raw and processed products have different pharmacological effects in clinical applications. To explore the chemical profile differences of components in A. tataricus processed with different methods, metabolomics methods based on ultra-high-performance liquid chromatography coupled with quadrupole time of flight mass spectrometry and gas chromatography-mass spectrometry were developed. Chemometrics strategy was applied to filter and screen the candidate compounds. The accuracy of differential markers was validated by back propagation neural network. The established methods showed that raw A. tataricus, honey-processed A. tataricus, vinegar-processed A. tataricus, and steamed A. tataricus were clearly divided into four groups, suggesting that the components were closely related to the processing methods. A total of 64 nonvolatile and 43 volatile compounds were identified in A. tataricus, and 22 nonvolatile and 12 volatile differential constituents were selected to distinguish the raw and processed A. tataricus. This study demonstrated that the metabolomics methods coupled with chemometrics were a comprehensive strategy to analyze the chemical profile differences and provided a reliable reference for quality evaluation of A. tataricus.

Chemometrics

10.1002/jssc.202100315

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Citations (10)

A validated LC–MS/MS assay for the simultaneous determination of periplocin and its two metabolites, periplocymarin and periplogenin in rat plasma: Application to a pharmacokinetic study

Journal of Pharmaceutical and Biomedical Analysis (2015)

Jun He Bo Fang TU Ya-ru John Teye Azietaku Ting Dou

Quantitative Analysis

10.1016/j.jpba.2015.06.008

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Citations (22)

A validated LC–MS/MS method for determination of periplogenin in rat plasma and its application in pharmacokinetic study

Journal of Chromatography B (2015)

Bo Fang Ting Dou Xingrui Wang Paul Owusu Donkor Huizi Ouyang

10.1016/j.jchromb.2015.03.017

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Citations (12)

UPLC-MS/MS Method for the Simultaneous Quantification of Eight Compounds in Rat Plasma and Its Application to a Pharmacokinetic Study after Oral Administration of Veratrum (Veratrum nigrum L.) Extract

BioMed Research International (2020)

Yuqi Fan Lulu Zhao Xuhua Huang Jiayuan Shen Wei Wang

Veratrum nigrum L. is a well-known traditional Chinese medicine with a lot of pharmacological activities including antihypertensive, anticancer, and antifungal effects. In the current experiment, a rapid and sensitive UPLC-MS/MS method that takes only 7 min run time has been established and validated for simultaneous determination of eight bioactive compounds including cyclopamine, jervine, veratramine, polydatin, quercetin, apigenin, resveratrol, and veratrosine in rat plasma. The chromatographic separation of analytes and internal standard was performed on a Phenyl-Hexyl column (

2.1 \times 100 mm

, 1.7 μm) with the mobile phase consisting of water (0.1% formic acid) and acetonitrile at a flow rate of 0.3 mL/min. An electrospray ionization (ESI) source was used to detect the samples in both positive and negative ion modes. The intra- and interday precisions of the compounds were less than 9.5% and the accuracy ranged from -10.8% to 10.4%. The extraction recoveries of the compounds were in the range of

85.1 \pm 1.5 %

102.6 \pm 8.0 %

, and the matrix effect ranged from

91.2 \pm 4.5 %

113.8 \pm 1.5 %

. According to the results of the stability test, the eight compounds have good stability under various conditions and the relative standard deviation (RSD) less than 13.2%. The pharmacokinetic parameters of the eight compounds in rat plasma after oral administration of Veratrum nigrum L. extract were successfully determined by the established UPLC-MS/MS method.

10.1155/2020/8883277

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Citations (5)

Pharmacokinetic comparison of 15 active compositions in rat plasma after oral administration of raw and honey‐processed Aster tataricus extracts

Journal of Separation Science (2020)

Dongyue Yang Songrui Wang Xuhua Huang Wenjuan Ma Zixiang Xue

Abstract A sensitive ultra‐high‐performance liquid chromatography–tandem mass spectrometry method was developed and validated to clarify pharmacokinetic properties of 15 compounds (quercetin, isorhamnetin, chlorogenic acid, isoquercitrin, caffeic acid, scopoletin, 7‐hydroxycoumarin, shionone, ferulic acid, kaempferol‐7‐O‐β‐ d ‐glucopyranoside, methyl caffeate, luteolin, kaempferol, epifriedelinol, and protocatechuic acid) in raw and honey‐processed Aster tataricus . Separation was carried out on an ACQUITY UPLC ® BEH C 18 column (2.1 × 100 mm, 1.7 μm) using a gradient elution with mobile phase constituting 0.1% formic acid‐water and 0.05% formic acid‐methanol. Quantitative analysis was performed using multiple reaction monitoring detection in both positive and negative ionization modes. Calibration curves showed good linearity ( r 2 > 0.991) over the corresponding concentration range. The intra‐ and interday precisions were within 10.1%, and accuracy ranged from −11.4 to 12.4%. The extraction recoveries and matrix effects were 78.1–100.0% and 81.1–113.7%, respectively. The analytes were stable under four storage conditions with relative standard deviations less than 12.6%. The validated method was successfully applied to compare the pharmacokinetic behaviors of raw and honey‐processed Aster tataricus for the first time. The results indicated that the areas under the curve (AUCs) of shionone, ferulic acid, and protocatechuic acid in honey‐processed A. tataricus group were significantly lower than that of raw A. tataricus group.

Chlorogenic Acid

Isorhamnetin

Protocatechuic acid

10.1002/jssc.202001020

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Citations (9)

Spectrum‐Effect Relationship Between Fingerprints and Anti‐Inflammatory and Antitussive Activities of Raw and Processed Mume Fructus Extracts

Biomedical Chromatography (2025)

Zhenguo Lv Wenhan Lin Omachi Daniel Ogaji Yang Liu Shuting Zhou

ABSTRACT Mume Fructus (MF), a representative substance in the field of medicine‐food homology, has been extensively utilized in clinical treatments and daily diets for its raw and processed forms. This study aimed to establish the spectrum‐effect relationship between ultra–high‐performance liquid chromatography coupled with quadrupole tandem time‐of‐flight mass spectrometry (UHPLC‐Q‐TOF‐MS/MS) fingerprints and anti‐inflammatory and antitussive activities of raw and processed MF extracts. In UHPLC‐Q‐TOF‐MS/MS fingerprints, a total of 21 common peaks were identified. Bioactivity assays demonstrated that the raw and processed MF extracts exhibited varying degrees of anti‐inflammatory and antitussive effects. Subsequently, the relevant pharmacologically active ingredients were screened by grey relation analysis and partial least squares regression. The results showed that nine components were associated with anti‐inflammatory effects, namely, citric acid, isochlorogenic acid B, isochlorogenic acid A, isochlorogenic acid C, fumaric acid, gallic acid, neochlorogenic acid, chlorogenic acid, and ononin. Additionally, three components were linked to antitussive activity: amygdalin, syringic acid, and succinic acid, respectively. This work developed a model combining UHPLC‐Q‐TOF‐MS/MS fingerprints with anti‐inflammatory and antitussive activities to study the spectrum‐effect relationship of raw and processed MF extracts. The findings provide a reference for the discovery of bioactive components and contribute to the clinical rationalization of medications.

Syringic acid

Chlorogenic Acid

Succinic acid

Anti-inflammatory

10.1002/bmc.70037

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