OBJECTIVE: To investigate the effects and mechanisms of arsenic trioxide (ATO) on human choroidal melanoma cell line OCM-1. METHODS: OCM-1 cells were cultured with 0.75 to 24.00 micromol/L arsenic trioxide for various durations, then cell viability was measured by MTT assay. The cell necrosis and apoptosis rates were observed by flow cytometry. The morphological changes of the cells were examined by electron microscopy. Glutathione peroxidase (GSH-Px) activities were tested. Mitochondrial membrane potential (MMP) was detected by confocal microscopy. RESULTS: Growth of OCM-1 cells was inhibited by ATO at concentrations of (1.5 to 24.0) micromol/L. However, there was no effect of 0.75 micromol/L ATO on the growth of OCM-1 cells. The inhibition showed both dose and time dependent effects (P < 0.05). The IC(50) was 16.8 micromol/L at 24 h. Flow cytometry analysis showed a positive correlation between the rate of cell necrosis and apoptosis and the concentration of ATO. The cell necrosis rates were higher than the cell apoptosis rates at various concentrations of ATO. OCM-1 cells cultured with ATO showed the classic morphologic characteristics of necrosis and apoptosis. GSH-Px activities and MMP decreased in a dose dependent manner. CONCLUSION: ATO inhibits the growth of OCM-1 cells. The mechanism of this effect is that ATO inhibits the GSH-Px activities, decreases the MMP and impairs mitochondrial energy synthesis, which induces necrosis and apoptosis of human choroidal melanoma COM-1 cells eventually.
The coordination of carbohydrate to metal ions is important because it may be involved in many biochemical processes. The synthesis and characterization of several novel lanthanide-erythritol complexes (TbCl3·1.5C4H10O4·H2O (TbE(I)), Pr(NO3)3·C4H10O4·2H2O (PrEN), Ce(NO3)3·C4H10O4·2H2O (CeEN), Y(NO3)3·C4H10O4·C2H5OH (YEN), Gd(NO3)3·C4H10O4·C2H5OH (GdEN)) and Tb(NO3)3·C4H10O4·C2H5OH (TbEN) are reported. The structures of these complexes in the solid state have been determined by X-ray diffraction. Erythritol is used as two bidentate ligands or as three hydroxyl group donor in these complexes. FTIR spectra indicate that two kinds of structures, with water and without water involved in the coordination sphere, were observed for lanthanide nitrate-erythritol complexes. FIR and THz spectra show the formation of metal ion-erythritol complexes. Luminescence spectra of Tb-erythritol complexes have the characteristics of the Tb ion.
To explore the correlation changes of syndrome and disease on pathology of gastric mucosa and expression of oncogene in chronic atrophic gastritis(CAG). The CAG model, which combined with syndrome and disease, were established. The model of insufficiency of the spleen, stagnation of the liver-qi and deficiency of the kidney separately were established with CAG, and correlative group were established as control. Pathological studies of every group were on the gastric mucoasa. The change of dynamics of cell proliferation was detected. Expression of PCNA was detected by immunoh is tochemical method. Expression of p53 oncogene was detected by in situ hybridization. Compared to the control group, every experimental group had atrophy of gastric mucosa, chronic inflamm ation, atypical hyperplasia, and the changes of dynam ics of cell proliferation. The expression of PCNA and P53 oncogene was higher. Am ong these groups, the changes in deficiency of the kidey were obvious. There have difference type of differentiation of symptoms and signs in CAG, in which deficiency of the kidney have essential correlation with the pathology of CAG.
Abstract Purpose Hypoxia induces abnormal expression of various long non-coding RNAs (LncRNAs) highly correlated with tumorigenesis. In this study, we identified CTD-2510F5.4, a hypoxia-induced LncRNA, based on microarray and TCGA analyses, and evaluated its impact on HCC prognosis, tumor microenvironment (TME), and drug efficacy. Methods We cultured Huh7 cells in a hypoxic chamber and detected CTD-2510F5.4 expression levels using RT-PCR analysis. Then we tested the effects of CTD-2510F5.4 overexpression on cell proliferation, invasion, and metastasis potential using CCK8, wound-healing, and transwell assays, respectively. We performed GO and Guilt-by-Association (GBA) correlation analysis to predict CTD-2510F5.4 functions. Besides, mutation signature, immune characteristics, and therapeutic response prediction between high- and low-CTD-2510F5.4 groups were further compared. Results Our results showed that CTD-2510F5.4 expression markedly increased under hypoxia and significantly promoted HCC cell proliferation, invasion, and metastasis. Functional enrichment analyses revealed that CTD-2510F5.4 is involved in cell proliferation and various tumor-related signaling pathways, including cell cycle, E2F targets, G2M checkpoint, and MYC targets V1. Patients with high CTD-2510F5.4 expression rates are preferentially associated with worse prognosis, higher TP53 mutation rates, higher infiltration by immune-suppressive regulatory cells, expressed immune checkpoints at elevated levels, and higher TIDE scores. The half-maximal inhibitory concentration (IC50) indicated that patients with low CTD-2510F5.4 expression are more responsive to immunotherapy and antiangiogenic targeted therapy, whereas those with high CTD-2510F5.4 expression are more sensitive to chemotherapy. Conclusion Our findings suggest that CTD-2510F5.4 could be a valuable biomarker for guiding the personalized treatment of HCC patients.
[Objective] The paper was to explore the microscopic structure of rabbit hair.[Method] Single rabbit hair with typical features was selected to observe its microscopic structure from tip to root,and its fiber diameter was also measured.[Result] The rabbit hair tip was constituted by scale layer and cortical layer,without medullary layer;the middle part was generally constituted by scale layer,cortical layer and medullary layer;the root had no medullary layer,and the scale layer was wheatear-shaped.This was the property of rabbit hair,which could be used for comparative studies with other animal fiber and species identification.Rabbit hair had developed medullary layer,and fiber diameter was positively related to column number of medullary cavity.The hair generally was single column,and coarse hair was multi-column.Single rabbit hair was the finest in the tip,coarse in the middle and tapering in the root.The diameter difference of various parts was large,the external growth characteristics was spindle-shaped.[Conclusion] Using biological microscope method to identify different animal fur and product species is more objective and simple.
To investigate the inhibitory effects of DEK/insulin‑like growth factor II mRNA binding protein 3 (IMP3) on epithelial‑mesenchymal transition (EMT) in colorectal carcinoma cells. SW620 and SW480 cell lines were selected. DEK‑interfering lentivirus was transfected to knockdown DEK expression. Subsequently, MTT assays and flow cytometry were utilized to measure cell viability, and apoptosis, respectively. Cell invasion was detected using a Transwell assay. Quantitative polymerase chain reaction and western blot analysis were used to detect the expression of E‑cadherin, vimentin, and matrix metalloproteinase (MMP)‑9. Compared with the blank control, cells transfected with DEK‑interfering lentivirus demonstrated a remarkable reduction in cell viability (P<0.05). The apoptotic rate in the DEK‑interfering lentivirus group was significantly enhanced compared with the blank control group (P<0.05). In the DEK‑interfering lentivirus group, the expression of E‑cadherin was significantly elevated (P<0.05), while the expression of vimentin and MMP‑9 were significantly reduced in both cell lines (P<0.05). The results of the present study demonstrated that EMT of colorectal carcinoma cells was partially mediated by DEK, which likely affected the invasive ability of colorectal carcinoma cells. In addition, cell proliferation and apoptosis were susceptible to DEK silencing. The current study has provided experimental evidence for the treatment of colorectal carcinoma using DEK silencing.
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