Xuelian Zhang

China-Japan Friendship Hospital

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Guibo Sun

Chinese Academy of Medical Sciences & Peking Union Medical College

Xiaobo Sun

Chinese Academy of Medical Sciences & Peking Union Medical College

Chenyang Zhang

Chinese Academy of Medical Sciences & Peking Union Medical College

Bin Zhang

State Administration of Traditional Chinese Medicine of the People's Republic of China

Weijie Xie

Peking University Shenzhen Hospital

Yun Luo

Chinese Academy of Medical Sciences & Peking Union Medical College

Ruiying Wang

Chinese Academy of Medical Sciences & Peking Union Medical College

Xi Dong

Chinese Academy of Medical Sciences & Peking Union Medical College

Ziru Dai

Chinese Academy of Medical Sciences & Peking Union Medical College

Shan Lu

Chinese Academy of Medical Sciences & Peking Union Medical College

Cooperative Institutions

Chinese Academy of Medical Sciences & Peking Union Medical College

Fudan University

Chinese Academy of Sciences

Shanghai Jiao Tong University

Peking University

Capital Medical University

Ministry of Education of the People's Republic of China

Southern Medical University

Huashan Hospital

Sun Yat-sen University

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Distribution of the natural history stages of pregnant women with chronic hepatitis B virus infection and its association with pregnancy complications and adverse pregnancy outcomes

Journal of Hepatology (2023)

Xuelian Zhang Yunfei Gao Jinna Li Xueru Yin Yaohua Hao

10.1016/s0168-8278(23)03214-2

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[An improved extraction and nonradioactive thin-layer chromatography detection method of mycolic acid].

PubMed (2023)

Siyue Xu Yuchang DI Mingzhe Chi Youwei Hu Xiao Zhang

Mycolic acids (MAs), i.e. 2-alkyl, 3-hydroxy long-chain fatty acids, are the hallmark of the cell envelope of Mycobacterium tuberculosis and are related with antibiotic resistance and host immune escape. Nowadays, they've become hot target of new anti-tuberculosis drugs. There are two main methods to detect MAs, 14C metabolic labeling thin-layer chromatography (TLC) and liquid chromatograph mass spectrometer (LC-MS). However, the user qualification of 14C or the lack of standards for LC-MS hampered the easy use of this method. TLC is a common way to analyze chemical substance and can be used to analyze MAs. In this study, we used tetrabutylammonium hydroxide and methyl iodide to hydrolyze and formylate MAs from mycobacterium cell wall. Subsequently, we used diethyl ether to extract methyl mycolate. By this method, we can easily extract and analyze MA in regular biological labs. The results demonstrated that this method could be used to compare MAs of different mycobacterium in different growth phases, MAs of mycobacteria treated by anti-tuberculosis drugs or MAs of mycobacterium mutants. Therefore, we can use this method as an initial validation for the changes of MAs in researches such as new drug screening without using radioisotope or when the standards are not available.

Mycolic acid

10.13345/j.cjb.220932

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Genetic Variants Associated with Lipid Profiles in Chinese Patients with Type 2 Diabetes

PLoS ONE (2015)

Xiaomu Kong Qi Zhao Xiaoyan Xing Bo Zhang Xuelian Zhang

Dyslipidemia is a strong risk factor for cardiovascular disease among patients with type 2 diabetes (T2D). The aim of this study was to identify lipid-related genetic variants in T2D patients of Han Chinese ancestry. Among 4,908 Chinese T2D patients who were not taking lipid-lowering medications, single nucleotide polymorphisms (SNPs) in seven genes previously found to be associated with lipid traits in genome-wide association studies conducted in populations of European ancestry (ABCA1, GCKR, BAZ1B, TOMM40, DOCK7, HNF1A, and HNF4A) were genotyped. After adjusting for multiple covariates, SNPs in ABCA1, GCKR, BAZ1B, TOMM40, and HNF1A were identified as significantly associated with triglyceride levels in T2D patients (P < 0.05). The associations between the SNPs in ABCA1 (rs3890182), GCKR (rs780094), and BAZ1B (rs2240466) remained significant even after correction for multiple testing (P = 8.85×10−3, 7.88×10−7, and 2.03×10−6, respectively). BAZ1B (rs2240466) also was associated with the total cholesterol level (P = 4.75×10−2). In addition, SNP rs157580 in TOMM40 was associated with the low-density lipoprotein cholesterol level (P = 6.94×10−3). Our findings confirm that lipid-related genetic loci are associated with lipid profiles in Chinese patients with type 2 diabetes.

Genome-wide Association Study

SNP

Dyslipidemia

HNF1A

Genetic Association

10.1371/journal.pone.0135145

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Construction of transferring vector of Marek^s disease virus vaccine strain CVI988 containing the virulent glycoprotein B and green fluorescent protein gene

Nongye shengwu jishu xuebao (2002)

Xuelian Zhang Weixing Fan Wei Rong Chen Pu-yan

Marek's disease

Strain (injury)

Source

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Ganoderma lucidum polysaccharides ameliorate lipopolysaccharide-induced acute pneumonia via inhibiting NRP1-mediated inflammation

Pharmaceutical Biology (2022)

Xuelian Zhang Daoshun Wu Yu Tian Xiangdong Chen Jin Lan

Context Ganoderma lucidum polysaccharides (GLP), from Ganoderma lucidum (Leyss. ex Fr.) Karst. (Ganodermataceae), are reported to have anti-inflammatory effects, including anti-neuroinflammation and anti-colitis. Nevertheless, the role of GLP in acute pneumonia is unknown.Objective To explore the protective role of GLP against LPS-induced acute pneumonia and investigate possible mechanisms.Materials and methods GLP were extracted and used for high-performance liquid chromatography (HPLC) analysis after acid hydrolysis and PMP derivatization. Sixty C57BL/6N male mice were randomly divided into six groups: Sham, Model, LPS + GLP (25, 50 and 100 mg/kg/d administered intragastrically for two weeks) and LPS + dexamethasone (6 mg/kg/d injected intraperitoneally for one week). Acute pneumonia mouse models were established by intratracheal injection of LPS. Haematoxylin and eosin (H&E) staining was examined to evaluate lung lesions. ELISA and quantitative real-time PCR were employed to assess inflammatory factors expression. Western blots were carried out to measure Neuropilin-1 expression and proteins related to apoptosis and autophagy.Results GLP suppressed inflammatory cell infiltration. In BALF, cell counts were 1.1 × 106 (model) and 7.1 × 105 (100 mg/kg). Release of GM-CSF and IL-6 was reduced with GLP (25, 50 and 100 mg/kg) treatment. The expression of genes IL-1β, IL-6, TNF-α and Saa3 was reduced. GLP treatment also suppressed the activation of Neuropilin-1 (NRP1), upregulated the levels of Bcl2/Bax and LC3 and led to downregulation of the ratio C-Caspase 3/Caspase 3 and P62 expression.Discussion and conclusions GLP could protect against LPS-induced acute pneumonia through multiple mechanisms: blocking the infiltration of inflammatory cells, inhibiting cytokine secretion, suppressing NRP1 activation and regulating pneumonocyte apoptosis and autophagy.

Proinflammatory cytokine

10.1080/13880209.2022.2142615

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Construction and Biological Characteristics of ClpC and ClpX Knock-down Strains in Mycobacterium smegmatis

Zhongguo shengwu gongcheng zazhi (2021)

Jia-cheng Bai Ming-zhe Chi Yawen Hu Meng Hao Xuelian Zhang

Mycobacterium smegmatis

Gene knockin

10.13523/j.cb.2103030

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Correlation of ACR and TcPO2 in diabetic kidney disease patients: A pilot study

Journal of Diabetes (2023)

Sun Jin Yang Huang Lan‐Hua Li Hao Hu Yuanyuan Liu

Abstract Objective Transcutaneous oxygen pressure (TcPO2) is used to assess microcirculation clinically; however, it is not widely available especially in rural hospital. The study was designed to explore potential alternatively biomarkers to assess microcirculation in diabetic kidney disease (DKD). Methods A total of 404 patients from Xuzhou first hospital were recruited according to the case records system. Patients were grouped via the ratio of albuminuria and creatinine (ACR; <30 mg/g, 30−300 mg/g, >300 mg/g). Biomarkers in different ACR groups were compared by analysis of variance. Correlation analysis was determined by Pearson or Spearman analysis and binary logistic regression. The receiver operating characteristics (ROC) curve was performed to elucidate the prediction effect of ACR on TcPO2. Results A total of 404 diabetic patients were recruited with 248 patients diagnosed as DKD and 156 non‐DKDs. Age and cystatin C were significantly higher in the ACR3 group compared with those in the ACR1 group, whereas glomerular filtration rate, low‐density lipoprotein cholesterol, and TcPO2 were markedly decreased in the ACR3 group ( p < .05). Frequency of low TcPO2 (<40 mm Hg) was markedly increased as increment of ACR stages with 30.2% in the ACR3 group ( p < .01). There was a negative correlation between TcPO2 and age, ACR, chronic kidney disease (CKD), fast blood glucose, diabetes mellitus (DM) duration, and diabetic neuropathy. Further, binary logistic regression showed ACR was an independent influence factor for low TcPO2. After adjusting for age, gender, hypertension, DM duration, body mass index, glycated hemoglobin, diabetic neuropathy, and CKD, ACR was still an independent influence factor for TcPO2 (odds ratio = 2.464, p < .01). The area under the ROC curve was 0.768 (95% confidence interval: 0.700–0.836, p < .001) for ACR. The analysis of ROC curves revealed a best cutoff for ACR was 75.25 mg/g and yielded a sensitivity of 71.7% and a specificity of 71.7%. Conclusions ACR could be used as an alternative biomarker for assessing microcirculation in DKD patients.

Glycated hemoglobin

Albuminuria

10.1111/1753-0407.13385

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Diketopiperazine-Type Alkaloids from a Deep-Sea-Derived Aspergillus puniceus Fungus and Their Effects on Liver X Receptor α

Journal of Natural Products (2019)

Liang Xiao Xuelian Zhang Xinhua Lu Zhihui Zheng Xuan Ma

Eight new diketopiperazine-type alkaloids including four oxepin-containing diketopiperazine-type alkaloids, oxepinamides H-K (1-4), and four 4-quinazolinone alkaloids, puniceloids A-D (5-8), together with two known analogues (9 and 10), were isolated from the culture broth extracts of the deep-sea-derived fungus Aspergillus puniceus SCSIO z021. Their structures were elucidated by spectroscopic analyses, and their absolute configurations were determined by Marfey's method along with comparison of their specific rotations and ECD spectra. The absolute configurations of 4 and 5 were further confirmed by a single-crystal X-ray diffraction analysis. Compounds 1-8 showed significant transcriptional activation of liver X receptor α with EC50 values of 1.7-50 μM, and 7 and 8 were the most potent agonists.

10.1021/acs.jnatprod.9b00055

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Cerebral function imaging of acupuncture treatment for stroke: A protocol for systematic review and meta-analysis

Research Square (Research Square) (2021)

Yihao Zhou Jing Shi Gan Huang Zhilin Huang Sifeng Feng

Abstract Background: Stroke is one of the most common causes of death and is the main cause of persistent and acquired disability in adults worldwide. Acupuncture is recommended as an alternative and complementary strategy for stroke treatment by the World Health Organization as it can significantly improve patients' quality of life. However, the central nervous system (CNS) mechanism of acupuncture treatment of stroke is unclear. The aim of this study is explore the effective pathway and action mechanism of acupuncture treatment for stroke on the CNS. Methods: The following databases will be searched by electronic methods: PubMed; Medline; Embase; Cochrane Library; Chinese National Knowledge Infrastructure; VIP Database; Wan-fang Data; Chinese Biomedical Database. All of them will be retrieved from the establishment date of the electronic database to December 2020, all included studies will be evaluated risk of bias by the Cochrane Handbook. Spatial coordinates of the Montreal Neurological Institute of activated brain regions will be the primary outcome. The systematic review will be conducted with the use of SDM v5.141 software for voxel meta-analysis in this study. Results: This study will obtain the correlation between the activated brain regions of acupuncture treatment for stroke. Conclusion: This study will explore the effective pathway and action mechanism of acupuncture, and provide a reliable scientific basis for the treatment of stroke by acupuncture. PROSPERO registration number: CRD42021231329.

Stroke

10.21203/rs.3.rs-344710/v1

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PRESERVATION OF THE CARDIAC FUNCTION IN INFARCTED RAT HEARTS BY THE TRANSPLANTATION OF ADIPOSE-DERIVED STEM CELLS WITH INJECTABLE FIBRIN SCAFFOLDS

Journal of the American College of Cardiology (2011)

Yi Fei Tong Xuelian Zhang

10.1016/s0735-1097(11)60384-7

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Citations (2)